ID   LUXB_VIBHA              Reviewed;         324 AA.
AC   P07739;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   16-JUN-2009, entry version 63.
DE   RecName: Full=Alkanal monooxygenase beta chain;
DE            EC=1.14.14.3;
DE   AltName: Full=Bacterial luciferase beta chain;
GN   Name=luxB;
OS   Vibrio harveyi.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Vibrio.
OX   NCBI_TaxID=669;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=ATCC 33843 / 392 / MAV;
RX   MEDLINE=86168191; PubMed=3514602;
RA   Johnston T.C., Thompson R.B., Baldwin T.O.;
RT   "Nucleotide sequence of the luxB gene of Vibrio harveyi and the
RT   complete amino acid sequence of the beta subunit of bacterial
RT   luciferase.";
RL   J. Biol. Chem. 261:4805-4811(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-12.
RX   MEDLINE=85207595; PubMed=3997817;
RA   Cohn D.H., Mileham A.J., Simon M.I., Nealson K.H., Rausch S.K.,
RA   Bonam D., Baldwin T.O.;
RT   "Nucleotide sequence of the luxA gene of Vibrio harveyi and the
RT   complete amino acid sequence of the alpha subunit of bacterial
RT   luciferase.";
RL   J. Biol. Chem. 260:6139-6146(1985).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
RX   MEDLINE=95275854; PubMed=7756289; DOI=10.1021/bi00020a002;
RA   Fisher A.J., Raushel F.M., Baldwin T.O., Rayment I.;
RT   "Three-dimensional structure of bacterial luciferase from Vibrio
RT   harveyi at 2.4-A resolution.";
RL   Biochemistry 34:6581-6586(1995).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
RX   MEDLINE=96355591; PubMed=8703001; DOI=10.1074/jbc.271.36.21956;
RA   Fisher A.J., Thompson T.B., Thoden J.B., Baldwin T.O., Rayment I.;
RT   "The 1.5-A resolution crystal structure of bacterial luciferase in low
RT   salt conditions.";
RL   J. Biol. Chem. 271:21956-21968(1996).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX   MEDLINE=97172895; PubMed=9020763; DOI=10.1021/bi962511x;
RA   Tanner J.J., Miller M.D., Wilson K.S., Tu S.C., Krause K.L.;
RT   "Structure of bacterial luciferase beta 2 homodimer: implications for
RT   flavin binding.";
RL   Biochemistry 36:665-672(1997).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
RX   MEDLINE=97160473; PubMed=9007973;
RA   Thoden J.B., Holden H.M., Fisher A.J., Sinclair J.F., Wesenberg G.,
RA   Baldwin T.O., Rayment I.;
RT   "Structure of the beta 2 homodimer of bacterial luciferase from Vibrio
RT   harveyi: X-ray analysis of a kinetic protein folding trap.";
RL   Protein Sci. 6:13-23(1997).
CC   -!- FUNCTION: Light-emitting reaction in luminous bacteria. The
CC       specific role of the beta subunit is unknown, but it is absolutely
CC       required for bioluminescence activity.
CC   -!- CATALYTIC ACTIVITY: RCHO + reduced FMN + O(2) = RCOOH + FMN +
CC       H(2)O + light.
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC   -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase
CC       family.
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DR   EMBL; M10961; AAA88686.1; -; Genomic_DNA.
DR   PIR; A23866; A23866.
DR   PDB; 1BRL; X-ray; 2.40 A; B/D=1-324.
DR   PDB; 1BSL; X-ray; 1.95 A; A/B=1-324.
DR   PDB; 1LUC; X-ray; 1.50 A; B=1-324.
DR   PDB; 1XKJ; X-ray; 2.50 A; A/B=1-324.
DR   PDBsum; 1BRL; -.
DR   PDBsum; 1BSL; -.
DR   PDBsum; 1LUC; -.
DR   PDBsum; 1XKJ; -.
DR   BRENDA; 1.14.14.3; 351.
DR   GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:EC.
DR   GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR018235; Bacterial_luciferase_CS.
DR   InterPro; IPR011251; Luciferase-like.
DR   InterPro; IPR016048; Luciferase-like_sub.
DR   InterPro; IPR002103; Luciferase_bac.
DR   Gene3D; G3DSA:3.20.20.30; Luciferase_like; 1.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   PRINTS; PR00089; LUCIFERASE.
DR   PROSITE; PS00494; BACTERIAL_LUCIFERASE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Flavoprotein; FMN;
KW   Luminescence; Monooxygenase; Oxidoreductase; Photoprotein.
FT   CHAIN         1    324       Alkanal monooxygenase beta chain.
FT                                /FTId=PRO_0000220181.
FT   STRAND        2      7
FT   STRAND       13     15
FT   HELIX        17     32
FT   TURN         33     35
FT   STRAND       38     41
FT   STRAND       46     49
FT   HELIX        55     65
FT   STRAND       70     77
FT   HELIX        83     97
FT   STRAND      101    106
FT   HELIX       111    116
FT   HELIX       124    141
FT   STRAND      142    144
FT   STRAND      148    150
FT   STRAND      168    174
FT   HELIX       177    185
FT   STRAND      190    192
FT   HELIX       198    215
FT   STRAND      225    232
FT   HELIX       236    254
FT   HELIX       260    270
FT   STRAND      271    275
FT   HELIX       276    290
FT   STRAND      293    298
FT   HELIX       305    318
SQ   SEQUENCE   324 AA;  36345 MW;  974FF37653C052D3 CRC64;
     MKFGLFFLNF MNSKRSSDQV IEEMLDTAHY VDQLKFDTLA VYENHFSNNG VVGAPLTVAG
     FLLGMTKNAK VASLNHVITT HHPVRVAEEA CLLDQMSEGR FAFGFSDCEK SADMRFFNRP
     TDSQFQLFSE CHKIINDAFT TGYCHPNNDF YSFPKISVNP HAFTEGGPAQ FVNATSKEVV
     EWAAKLGLPL VFRWDDSNAQ RKEYAGLYHE VAQAHGVDVS QVRHKLTLLV NQNVDGEAAR
     AEARVYLEEF VRESYSNTDF EQKMGELLSE NAIGTYEEST QAARVAIECC GAADLLMSFE
     SMEDKAQQRA VIDVVNANIV KYHS
//