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Protein

Alkanal monooxygenase beta chain

Gene

luxB

Organism
Vibrio harveyi (Beneckea harveyi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.

Catalytic activityi

RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase, Photoprotein

Keywords - Biological processi

Luminescence

Keywords - Ligandi

Flavoprotein, FMN

Names & Taxonomyi

Protein namesi
Recommended name:
Alkanal monooxygenase beta chain (EC:1.14.14.3)
Alternative name(s):
Bacterial luciferase beta chain
Gene namesi
Name:luxB
OrganismiVibrio harveyi (Beneckea harveyi)
Taxonomic identifieri669 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Alkanal monooxygenase beta chainPRO_0000220181Add
BLAST

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.

Protein-protein interaction databases

MINTiMINT-1518842.
STRINGi338187.VIBHAR_06241.

Structurei

Secondary structure

1
324
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Beta strandi13 – 153Combined sources
Helixi17 – 3216Combined sources
Turni33 – 353Combined sources
Beta strandi38 – 414Combined sources
Beta strandi46 – 494Combined sources
Helixi55 – 6511Combined sources
Beta strandi67 – 7711Combined sources
Helixi78 – 803Combined sources
Helixi83 – 9614Combined sources
Beta strandi101 – 1066Combined sources
Helixi111 – 1166Combined sources
Helixi121 – 1233Combined sources
Helixi124 – 14118Combined sources
Beta strandi142 – 1443Combined sources
Beta strandi147 – 1504Combined sources
Beta strandi155 – 1573Combined sources
Beta strandi170 – 1734Combined sources
Helixi177 – 18610Combined sources
Beta strandi190 – 1923Combined sources
Helixi198 – 21417Combined sources
Helixi219 – 2213Combined sources
Beta strandi225 – 2328Combined sources
Helixi236 – 25419Combined sources
Turni255 – 2573Combined sources
Helixi260 – 27011Combined sources
Beta strandi271 – 2755Combined sources
Helixi276 – 29015Combined sources
Beta strandi293 – 2986Combined sources
Helixi305 – 31713Combined sources
Helixi319 – 3246Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BRLX-ray2.40B/D1-324[»]
1BSLX-ray1.95A/B1-324[»]
1LUCX-ray1.50B1-324[»]
1XKJX-ray2.50A/B1-324[»]
3FGCX-ray2.30B/D1-324[»]
ProteinModelPortaliP07739.
SMRiP07739. Positions 1-324.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07739.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.30. 2 hits.
InterProiIPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like_dom.
IPR002103. Luciferase_bac.
[Graphical view]
PfamiPF00296. Bac_luciferase. 1 hit.
[Graphical view]
PRINTSiPR00089. LUCIFERASE.
SUPFAMiSSF51679. SSF51679. 1 hit.
PROSITEiPS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFGLFFLNF MNSKRSSDQV IEEMLDTAHY VDQLKFDTLA VYENHFSNNG
60 70 80 90 100
VVGAPLTVAG FLLGMTKNAK VASLNHVITT HHPVRVAEEA CLLDQMSEGR
110 120 130 140 150
FAFGFSDCEK SADMRFFNRP TDSQFQLFSE CHKIINDAFT TGYCHPNNDF
160 170 180 190 200
YSFPKISVNP HAFTEGGPAQ FVNATSKEVV EWAAKLGLPL VFRWDDSNAQ
210 220 230 240 250
RKEYAGLYHE VAQAHGVDVS QVRHKLTLLV NQNVDGEAAR AEARVYLEEF
260 270 280 290 300
VRESYSNTDF EQKMGELLSE NAIGTYEEST QAARVAIECC GAADLLMSFE
310 320
SMEDKAQQRA VIDVVNANIV KYHS
Length:324
Mass (Da):36,345
Last modified:February 1, 1995 - v2
Checksum:i974FF37653C052D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10961 Genomic DNA. Translation: AAA88686.1.
PIRiA23866.
RefSeqiWP_010991820.1. NZ_LOSK01000002.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10961 Genomic DNA. Translation: AAA88686.1.
PIRiA23866.
RefSeqiWP_010991820.1. NZ_LOSK01000002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BRLX-ray2.40B/D1-324[»]
1BSLX-ray1.95A/B1-324[»]
1LUCX-ray1.50B1-324[»]
1XKJX-ray2.50A/B1-324[»]
3FGCX-ray2.30B/D1-324[»]
ProteinModelPortaliP07739.
SMRiP07739. Positions 1-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1518842.
STRINGi338187.VIBHAR_06241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP07739.

Family and domain databases

Gene3Di3.20.20.30. 2 hits.
InterProiIPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like_dom.
IPR002103. Luciferase_bac.
[Graphical view]
PfamiPF00296. Bac_luciferase. 1 hit.
[Graphical view]
PRINTSiPR00089. LUCIFERASE.
SUPFAMiSSF51679. SSF51679. 1 hit.
PROSITEiPS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the luxB gene of Vibrio harveyi and the complete amino acid sequence of the beta subunit of bacterial luciferase."
    Johnston T.C., Thompson R.B., Baldwin T.O.
    J. Biol. Chem. 261:4805-4811(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 33843 / 392 / MAV.
  2. "Nucleotide sequence of the luxA gene of Vibrio harveyi and the complete amino acid sequence of the alpha subunit of bacterial luciferase."
    Cohn D.H., Mileham A.J., Simon M.I., Nealson K.H., Rausch S.K., Bonam D., Baldwin T.O.
    J. Biol. Chem. 260:6139-6146(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-12.
  3. "Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4-A resolution."
    Fisher A.J., Raushel F.M., Baldwin T.O., Rayment I.
    Biochemistry 34:6581-6586(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
  4. "The 1.5-A resolution crystal structure of bacterial luciferase in low salt conditions."
    Fisher A.J., Thompson T.B., Thoden J.B., Baldwin T.O., Rayment I.
    J. Biol. Chem. 271:21956-21968(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
  5. "Structure of bacterial luciferase beta 2 homodimer: implications for flavin binding."
    Tanner J.J., Miller M.D., Wilson K.S., Tu S.C., Krause K.L.
    Biochemistry 36:665-672(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
  6. "Structure of the beta 2 homodimer of bacterial luciferase from Vibrio harveyi: X-ray analysis of a kinetic protein folding trap."
    Thoden J.B., Holden H.M., Fisher A.J., Sinclair J.F., Wesenberg G., Baldwin T.O., Rayment I.
    Protein Sci. 6:13-23(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).

Entry informationi

Entry nameiLUXB_VIBHA
AccessioniPrimary (citable) accession number: P07739
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.