P07739 (LUXB_VIBHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkanal monooxygenase beta chain
EC=1.14.14.3

Alternative name(s):
Bacterial luciferase beta chain

Gene names

Name:

luxB

Organism

Vibrio harveyi (Beneckea harveyi)

Taxonomic identifier

669 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.
Catalytic activity	RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.
Subunit structure	Heterodimer of an alpha and a beta chain.
Sequence similarities	Belongs to the bacterial luciferase oxidoreductase family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanal monooxygenase (FMN-linked) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 324

324

Alkanal monooxygenase beta chain

PRO_0000220181

Secondary structure

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324

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Beta strand

13 – 15

3

Helix

17 – 32

16

Turn

33 – 35

3

Beta strand

38 – 41

4

Beta strand

46 – 49

4

Helix

55 – 65

11

Beta strand

70 – 77

8

Helix

83 – 97

15

Beta strand

101 – 106

6

Helix

111 – 116

6

Helix

124 – 141

18

Beta strand

142 – 144

3

Beta strand

148 – 150

3

Beta strand

168 – 174

7

Helix

177 – 185

9

Beta strand

190 – 192

3

Helix

198 – 215

18

Beta strand

225 – 232

8

Helix

236 – 254

19

Helix

260 – 270

11

Beta strand

271 – 275

5

Helix

276 – 290

15

Beta strand

293 – 298

6

Helix

305 – 318

14

Sequences

	Sequence		Length	Mass (Da)
	P07739 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 974FF37653C052D3	FASTA	324	36,345
	10 20 30 40 50 60 MKFGLFFLNF MNSKRSSDQV IEEMLDTAHY VDQLKFDTLA VYENHFSNNG VVGAPLTVAG 70 80 90 100 110 120 FLLGMTKNAK VASLNHVITT HHPVRVAEEA CLLDQMSEGR FAFGFSDCEK SADMRFFNRP 130 140 150 160 170 180 TDSQFQLFSE CHKIINDAFT TGYCHPNNDF YSFPKISVNP HAFTEGGPAQ FVNATSKEVV 190 200 210 220 230 240 EWAAKLGLPL VFRWDDSNAQ RKEYAGLYHE VAQAHGVDVS QVRHKLTLLV NQNVDGEAAR 250 260 270 280 290 300 AEARVYLEEF VRESYSNTDF EQKMGELLSE NAIGTYEEST QAARVAIECC GAADLLMSFE 310 320 SMEDKAQQRA VIDVVNANIV KYHS

References

[1]	"Nucleotide sequence of the luxB gene of Vibrio harveyi and the complete amino acid sequence of the beta subunit of bacterial luciferase." Johnston T.C., Thompson R.B., Baldwin T.O. J. Biol. Chem. 261:4805-4811(1986) [PubMed: 3514602] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 33843 / 392 / MAV.
[2]	"Nucleotide sequence of the luxA gene of Vibrio harveyi and the complete amino acid sequence of the alpha subunit of bacterial luciferase." Cohn D.H., Mileham A.J., Simon M.I., Nealson K.H., Rausch S.K., Bonam D., Baldwin T.O. J. Biol. Chem. 260:6139-6146(1985) [PubMed: 3997817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-12.
[3]	"Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4-A resolution." Fisher A.J., Raushel F.M., Baldwin T.O., Rayment I. Biochemistry 34:6581-6586(1995) [PubMed: 7756289] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
[4]	"The 1.5-A resolution crystal structure of bacterial luciferase in low salt conditions." Fisher A.J., Thompson T.B., Thoden J.B., Baldwin T.O., Rayment I. J. Biol. Chem. 271:21956-21968(1996) [PubMed: 8703001] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
[5]	"Structure of bacterial luciferase beta 2 homodimer: implications for flavin binding." Tanner J.J., Miller M.D., Wilson K.S., Tu S.C., Krause K.L. Biochemistry 36:665-672(1997) [PubMed: 9020763] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[6]	"Structure of the beta 2 homodimer of bacterial luciferase from Vibrio harveyi: X-ray analysis of a kinetic protein folding trap." Thoden J.B., Holden H.M., Fisher A.J., Sinclair J.F., Wesenberg G., Baldwin T.O., Rayment I. Protein Sci. 6:13-23(1997) [PubMed: 9007973] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M10961 Genomic DNA. Translation: AAA88686.1.

PIR

A23866.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BRL	X-ray	2.40	B/D	1-324	[»]
1BSL	X-ray	1.95	A/B	1-324	[»]
1LUC	X-ray	1.50	B	1-324	[»]
1XKJ	X-ray	2.50	A/B	1-324	[»]
3FGC	X-ray	2.30	B/D	1-324	[»]

ProteinModelPortal

P07739.

SMR

P07739. Positions 1-324.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like_dom.
IPR002103. Luciferase_bac.
[Graphical view]

Gene3D

G3DSA:3.20.20.30. Luciferase_like. 1 hit.

Pfam

PF00296. Bac_luciferase. 1 hit.
[Graphical view]

PRINTS

PR00089. LUCIFERASE.

SUPFAM

SSF51679. Luciferase_like. 1 hit.

PROSITE

PS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LUXB_VIBHA

Accession

Primary (citable) accession number: P07739

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	February 1, 1995
Last modified:	November 16, 2011
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program