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P07736

- RNU1_USTSP

UniProt

P07736 - RNU1_USTSP

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Protein

Guanyl-specific ribonuclease U1

Gene
N/A
Organism
Ustilago sphaerogena (Smut fungus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei37 – 371By similarity
Active sitei57 – 571Proton acceptorBy similarity
Active sitei92 – 921Proton donorBy similarity

GO - Molecular functioni

  1. endoribonuclease activity Source: InterPro
  2. ribonuclease T1 activity Source: UniProtKB-EC
  3. RNA binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Guanyl-specific ribonuclease U1 (EC:3.1.27.3)
Short name:
RNase U1
OrganismiUstilago sphaerogena (Smut fungus)
Taxonomic identifieri5271 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 105105Guanyl-specific ribonuclease U1PRO_0000137376Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11Pyrrolidone carboxylic acid
Disulfide bondi8 ↔ 1031 Publication
Disulfide bondi51 ↔ 871 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Structurei

3D structure databases

ProteinModelPortaliP07736.
SMRiP07736. Positions 32-104.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease N1/T1 family.Curated

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

P07736-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
QGGVSVNCGG TYYSSTQVNR AINNAKSGQY SSTGYPHTYN NYEGFDFSDY
60 70 80 90 100
CDGPYKEYPL KTSSSGYTGG SPGADRVVYD SNDGTFCGAI THTGASGNNF

VQCSY
Length:105
Mass (Da):11,157
Last modified:February 1, 1994 - v2
Checksum:i6AB6C3117A4B727A
GO

Sequence databases

PIRiA41445.

Cross-referencesi

Sequence databases

PIRi A41445.

3D structure databases

ProteinModelPortali P07736.
SMRi P07736. Positions 32-104.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.450.30. 1 hit.
InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view ]
Pfami PF00545. Ribonuclease. 1 hit.
[Graphical view ]
SUPFAMi SSF53933. SSF53933. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The amino acid sequence of ribonuclease U1, a guanine-specific ribonuclease from the fungus Ustilago sphaerogena."
    Takahashi K., Hashimoto J.
    J. Biochem. 103:313-320(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.

Entry informationi

Entry nameiRNU1_USTSP
AccessioniPrimary (citable) accession number: P07736
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1994
Last modified: October 1, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3