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P07736

- RNU1_USTSP

UniProt

P07736 - RNU1_USTSP

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Protein
Guanyl-specific ribonuclease U1
Gene
N/A
Organism
Ustilago sphaerogena (Smut fungus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei37 – 371 By similarity
Active sitei57 – 571Proton acceptor By similarity
Active sitei92 – 921Proton donor By similarity

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. endoribonuclease activity Source: InterPro
  3. ribonuclease T1 activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanyl-specific ribonuclease U1 (EC:3.1.27.3)
    Short name:
    RNase U1
    OrganismiUstilago sphaerogena (Smut fungus)
    Taxonomic identifieri5271 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 105105Guanyl-specific ribonuclease U1
    PRO_0000137376Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11Pyrrolidone carboxylic acid
    Disulfide bondi8 ↔ 1031 Publication
    Disulfide bondi51 ↔ 871 Publication

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Structurei

    3D structure databases

    ProteinModelPortaliP07736.
    SMRiP07736. Positions 32-104.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.10.450.30. 1 hit.
    InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view]
    PfamiPF00545. Ribonuclease. 1 hit.
    [Graphical view]
    SUPFAMiSSF53933. SSF53933. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P07736-1 [UniParc]FASTAAdd to Basket

    « Hide

    QGGVSVNCGG TYYSSTQVNR AINNAKSGQY SSTGYPHTYN NYEGFDFSDY    50
    CDGPYKEYPL KTSSSGYTGG SPGADRVVYD SNDGTFCGAI THTGASGNNF 100
    VQCSY 105
    Length:105
    Mass (Da):11,157
    Last modified:February 1, 1994 - v2
    Checksum:i6AB6C3117A4B727A
    GO

    Sequence databases

    PIRiA41445.

    Cross-referencesi

    Sequence databases

    PIRi A41445.

    3D structure databases

    ProteinModelPortali P07736.
    SMRi P07736. Positions 32-104.
    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.10.450.30. 1 hit.
    InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view ]
    Pfami PF00545. Ribonuclease. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53933. SSF53933. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The amino acid sequence of ribonuclease U1, a guanine-specific ribonuclease from the fungus Ustilago sphaerogena."
      Takahashi K., Hashimoto J.
      J. Biochem. 103:313-320(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.

    Entry informationi

    Entry nameiRNU1_USTSP
    AccessioniPrimary (citable) accession number: P07736
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: February 1, 1994
    Last modified: April 16, 2014
    This is version 62 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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