P07736 (RNU1_USTSP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Guanyl-specific ribonuclease U1 Short name=RNase U1 EC=3.1.27.3
Organism	Ustilago sphaerogena (Smut fungus)
Taxonomic identifier	5271 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.
Sequence similarities	Belongs to the ribonuclease N1/T1 family.

Ontologies

Keywords
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro endoribonuclease activity Inferred from electronic annotation. Source: InterPro ribonuclease T1 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 105	105	Guanyl-specific ribonuclease U1		PRO_0000137376
Sites
	Active site	37	1	By similarity
	Active site	57	1	Proton acceptor By similarity
	Active site	92	1	Proton donor By similarity
Amino acid modifications
	Modified residue	1	1	Pyrrolidone carboxylic acid
	Disulfide bond	8 ↔ 103		Ref.1
	Disulfide bond	51 ↔ 87		Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07736 [UniParc]. Last modified February 1, 1994. Version 2. Checksum: 6AB6C3117A4B727A	FASTA	105	11,157
	10 20 30 40 50 60 QGGVSVNCGG TYYSSTQVNR AINNAKSGQY SSTGYPHTYN NYEGFDFSDY CDGPYKEYPL 70 80 90 100 KTSSSGYTGG SPGADRVVYD SNDGTFCGAI THTGASGNNF VQCSY

References

[1]	"The amino acid sequence of ribonuclease U1, a guanine-specific ribonuclease from the fungus Ustilago sphaerogena." Takahashi K., Hashimoto J. J. Biochem. 103:313-320(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
PIR	A41445.
3D structure databases
ProteinModelPortal	P07736.
SMR	P07736. Positions 32-104.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.10.450.30. 1 hit.
InterPro	IPR000026. Gua-sp_ribonuclease_N1/T1. IPR016191. Ribonuclease/ribotoxin. [Graphical view]
Pfam	PF00545. Ribonuclease. 1 hit. [Graphical view]
SUPFAM	SSF53933. Ribonuclease/ribotoxin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNU1_USTSP

Accession

Primary (citable) accession number: P07736

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program