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Protein

Glycine receptor subunit alpha-1

Gene

Glra1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:1716350, PubMed:11981023, PubMed:2483325, PubMed:3037383). Channel opening is also triggered by taurine and beta-alanine (PubMed:1716350). Channel characteristics depend on the subunit composition; heteropentameric channels are activated by lower glycine levels and display faster desensitization. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Channel activity is potentiated by ethanol (By similarity). Potentiation of channel activity by intoxicating levels ot ethanol contribute to the sedative effects of ethanol (By similarity).By similarity4 Publications

Enzyme regulationi

Inhibited by strychnine (PubMed:1716350, PubMed:2483325, PubMed:3037383). Inhibited by picrotoxin (PubMed:2483325).3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi220 – 2201ZincBy similarity
Metal bindingi222 – 2221ZincBy similarity
Metal bindingi243 – 2431ZincBy similarity
Sitei289 – 2891Important for obstruction of the ion pore in the closed conformationBy similarity

GO - Molecular functioni

  • extracellular-glycine-gated chloride channel activity Source: UniProtKB
  • extracellular-glycine-gated ion channel activity Source: RGD
  • glycine binding Source: UniProtKB
  • taurine binding Source: UniProtKB
  • transmitter-gated ion channel activity Source: InterPro
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • acrosome reaction Source: Ensembl
  • action potential Source: Ensembl
  • adult walking behavior Source: Ensembl
  • anion transport Source: RGD
  • cellular response to amino acid stimulus Source: UniProtKB
  • cellular response to ethanol Source: UniProtKB
  • cellular response to zinc ion Source: UniProtKB
  • chloride transport Source: UniProtKB
  • inhibitory postsynaptic potential Source: UniProtKB
  • ion transport Source: UniProtKB
  • muscle contraction Source: UniProtKB
  • negative regulation of transmission of nerve impulse Source: UniProtKB
  • neuromuscular process controlling posture Source: Ensembl
  • neuropeptide signaling pathway Source: UniProtKB
  • positive regulation of acrosome reaction Source: Ensembl
  • protein heterooligomerization Source: RGD
  • protein homooligomerization Source: RGD
  • regulation of respiratory gaseous exchange by neurological system process Source: Ensembl
  • response to alcohol Source: UniProtKB
  • righting reflex Source: Ensembl
  • startle response Source: UniProtKB
  • synaptic transmission, glycinergic Source: RGD
  • visual perception Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit alpha-1
Alternative name(s):
Glycine receptor 48 kDa subunit
Glycine receptor strychnine-binding subunit
Gene namesi
Name:Glra1
Synonyms:Glyr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2704. Glra1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 250222ExtracellularBy similarityAdd
BLAST
Transmembranei251 – 27222Helical; Name=1By similarityAdd
BLAST
Topological domaini273 – 2775CytoplasmicBy similarity
Transmembranei278 – 29821Helical; Name=2By similarityAdd
BLAST
Topological domaini299 – 30911ExtracellularBy similarityAdd
BLAST
Transmembranei310 – 33021Helical; Name=3By similarityAdd
BLAST
Topological domaini331 – 42595CytoplasmicBy similarityAdd
BLAST
Transmembranei426 – 44621Helical; Name=4By similarityAdd
BLAST
Topological domaini447 – 45711ExtracellularBy similarityAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • chloride channel complex Source: UniProtKB-KW
  • dendrite Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: MGI
  • external side of plasma membrane Source: MGI
  • inhibitory synapse Source: Ensembl
  • integral component of plasma membrane Source: UniProtKB
  • intracellular membrane-bounded organelle Source: UniProtKB
  • membrane Source: MGI
  • neuronal cell body Source: UniProtKB
  • neuron projection Source: UniProtKB
  • perikaryon Source: UniProtKB-SubCell
  • postsynaptic membrane Source: UniProtKB-SubCell
  • synapse Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3392921.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 28281 PublicationAdd
BLAST
Chaini29 – 457429Glycine receptor subunit alpha-1PRO_0000000414Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi66 – 661N-linked (GlcNAc...)Curated
Disulfide bondi166 ↔ 180By similarity
Disulfide bondi226 ↔ 237By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

PTM databases

iPTMnetiP07727.

Expressioni

Tissue specificityi

Detected in spinal cord (at protein level) (PubMed:2483325, PubMed:6286620). Detected in spinal cord and brain stem (PubMed:3037383).3 Publications

Gene expression databases

ExpressionAtlasiP07727. baseline and differential.

Interactioni

Subunit structurei

Homopentamer (in vitro). Interacts with GLRB to form heteropentameric channels; this is probably the predominant form in vivo. Heteropentamer composed of two GLRA1 and three GLRB. Heteropentamer composed of three GLRA1 and two GLRB. Both homopentamers and heteropentamers form functional ion channels, but their characteristics are subtly different.By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-48879N.

Chemistry

BindingDBiP07727.

Structurei

3D structure databases

ProteinModelPortaliP07727.
SMRiP07727. Positions 277-337.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni230 – 2356Strychnine-bindingBy similarity1 Publication

Domaini

The channel pore is formed by pentameric assembly of the second transmembrane domain from all five subunits. Channel opening is effected by an outward rotation of the transmembrane domains that increases the diameter of the pore.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiP07727.
KOiK05193.
PhylomeDBiP07727.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR008128. Glycine_rcpt_A1.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR01674. GLYRALPHA1.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P07727-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYSFNTLRFY LWETIVFFSL AASKEADAAR SAPKPMSPSD FLDKLMGRTS
60 70 80 90 100
GYDARIRPNF KGPPVNVSCN IFINSFGSIA ETTMDYRVNI FLRQQWNDPR
110 120 130 140 150
LAYNEYPDDS LDLDPSMLDS IWKPDLFFAN EKGAHFHEIT TDNKLLRISR
160 170 180 190 200
NGNVLYSIRI TLTLACPMDL KNFPMDVQTC IMQLESFGYT MNDLIFEWQE
210 220 230 240 250
QGAVQVADGL TLPQFILKEE KDLRYCTKHY NTGKFTCIEA RFHLERQMGY
260 270 280 290 300
YLIQMYIPSL LIVILSWISF WINMDAAPAR VGLGITTVLT MTTQSSGSRA
310 320 330 340 350
SLPKVSYVKA IDIWMAVCLL FVFSALLEYA AVNFVSRQHK ELLRFRRKRR
360 370 380 390 400
HHKSPMLNLF QDDEGGEGRF NFSAYGMGPA CLQAKDGISV KGANNNNTTN
410 420 430 440 450
PAPAPSKSPE EMRKLFIQRA KKIDKISRIG FPMAFLIFNM FYWIIYKIVR

REDVHNK
Length:457
Mass (Da):52,617
Last modified:December 8, 2000 - v4
Checksum:i0379C33A83C71FAA
GO
Isoform b (identifier: P07727-2) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     354-361: Missing.

Show »
Length:449
Mass (Da):51,686
Checksum:i3183E1304DA77B1E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261A → V in CAA68378 (PubMed:3037383).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei354 – 3618Missing in isoform b. 2 PublicationsVSP_000081

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00833 mRNA. Translation: BAA00707.1.
X55246 mRNA. Translation: CAA38987.1.
M63915 mRNA. Translation: AAA63490.1.
AJ310835 mRNA. Translation: CAC35979.1.
Y00276 mRNA. Translation: CAA68378.1.
PIRiJN0014.
S20662.
RefSeqiNP_037265.1. NM_013133.1. [P07727-2]
XP_006246440.1. XM_006246378.2. [P07727-1]
UniGeneiRn.10109.

Genome annotation databases

EnsembliENSRNOT00000063889; ENSRNOP00000063384; ENSRNOG00000013588. [P07727-2]
GeneIDi25674.
KEGGirno:25674.
UCSCiRGD:2704. rat. [P07727-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00833 mRNA. Translation: BAA00707.1.
X55246 mRNA. Translation: CAA38987.1.
M63915 mRNA. Translation: AAA63490.1.
AJ310835 mRNA. Translation: CAC35979.1.
Y00276 mRNA. Translation: CAA68378.1.
PIRiJN0014.
S20662.
RefSeqiNP_037265.1. NM_013133.1. [P07727-2]
XP_006246440.1. XM_006246378.2. [P07727-1]
UniGeneiRn.10109.

3D structure databases

ProteinModelPortaliP07727.
SMRiP07727. Positions 277-337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48879N.

Chemistry

BindingDBiP07727.
ChEMBLiCHEMBL3392921.

PTM databases

iPTMnetiP07727.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000063889; ENSRNOP00000063384; ENSRNOG00000013588. [P07727-2]
GeneIDi25674.
KEGGirno:25674.
UCSCiRGD:2704. rat. [P07727-1]

Organism-specific databases

CTDi2741.
RGDi2704. Glra1.

Phylogenomic databases

HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiP07727.
KOiK05193.
PhylomeDBiP07727.

Miscellaneous databases

NextBioi607619.
PROiP07727.

Gene expression databases

ExpressionAtlasiP07727. baseline and differential.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR008128. Glycine_rcpt_A1.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR01674. GLYRALPHA1.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Functional properties of strychnine-sensitive glycine receptors expressed in Xenopus oocytes injected with a single mRNA."
    Akagi H., Hirai K., Hishinuma F.
    Neurosci. Res. 11:28-40(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION.
  2. "Alternative splicing generates two variants of the alpha 1 subunit of the inhibitory glycine receptor."
    Malosio M.L., Grenningloh G., Kuhse J., Schmieden V., Schmitt B., Prior P., Betz H.
    J. Biol. Chem. 266:2048-2053(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), ALTERNATIVE SPLICING.
    Strain: Wistar.
    Tissue: Spinal cord.
  3. "Openings of the rat recombinant alpha 1 homomeric glycine receptor as a function of the number of agonist molecules bound."
    Beato M., Groot-Kormelink P.J., Colquhoun D., Sivilotti L.G.
    J. Gen. Physiol. 119:443-466(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  4. "Functional chloride channels by mammalian cell expression of rat glycine receptor subunit."
    Sontheimer H., Becker C.M., Pritchett D.B., Schofield P.R., Grenningloh G., Kettenmann H., Betz H., Seeburg P.H.
    Neuron 2:1491-1497(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-30, FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "The strychnine-binding subunit of the glycine receptor shows homology with nicotinic acetylcholine receptors."
    Grenningloh G., Rienitz A., Schmitt B., Methfessel C., Zensen M., Beyreuther K., Gundelfinger E.D., Betz H.
    Nature 328:215-220(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 23-457 (ISOFORM B), PROTEIN SEQUENCE OF 29-49, FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Wistar.
  6. "Purification by affinity chromatography of the glycine receptor of rat spinal cord."
    Pfeiffer F., Graham D., Betz H.
    J. Biol. Chem. 257:9389-9393(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, SUBUNIT, TISSUE SPECIFICITY.
  7. "Localization of the strychnine binding site on the 48-kilodalton subunit of the glycine receptor."
    Ruiz-Gomez A., Morato E., Garcia-Calvo M., Valdivieso F., Mayor F. Jr.
    Biochemistry 29:7033-7040(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRYCHNINE BINDING SITE.
  8. "Kinetic analysis of recombinant mammalian alpha(1) and alpha(1)beta glycine receptor channels."
    Mohammadi B., Krampfl K., Cetinkaya C., Moschref H., Grosskreutz J., Dengler R., Bufler J.
    Eur. Biophys. J. 32:529-536(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GLRB.

Entry informationi

Entry nameiGLRA1_RAT
AccessioniPrimary (citable) accession number: P07727
Secondary accession number(s): Q546L6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: December 8, 2000
Last modified: May 11, 2016
This is version 159 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The alpha subunit binds strychnine.3 Publications

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.