P07727 (GLRA1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine receptor subunit alpha-1

Alternative name(s):
Glycine receptor 48 kDa subunit
Glycine receptor strychnine-binding subunit

Gene names

Name:	Glra1
Synonyms:	Glyr

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
Subunit structure	Pentamer composed of alpha and beta subunits.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Miscellaneous	The alpha subunit binds strychnine.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA1 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ionic channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	muscle contraction Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transmission of nerve impulse Inferred from sequence or structural similarity. Source: UniProtKB neuropeptide signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB protein heterooligomerization Inferred from direct assay. Source: RGD protein homooligomerization Inferred from direct assay. Source: RGD startle response Inferred from sequence or structural similarity. Source: UniProtKB synaptic transmission, glycinergic Inferred from direct assay. Source: RGD
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from direct assay. Source: MGI external side of plasma membrane Inferred from direct assay. Source: MGI integral to plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB membrane fraction Inferred from direct assay. Source: MGI postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	extracellular-glycine-gated chloride channel activity Inferred from sequence or structural similarity. Source: UniProtKB glycine binding Inferred from sequence or structural similarity. Source: UniProtKB receptor activity Inferred from electronic annotation. Source: UniProtKB-KW taurine binding Inferred from sequence or structural similarity. Source: UniProtKB transmitter-gated ion channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P07727-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: P07727-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     354-361: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Ref.5
	Chain	29 – 457	429	Glycine receptor subunit alpha-1		PRO_0000000414
Regions
	Topological domain	29 – 247	219	Extracellular Probable
	Transmembrane	248 – 274	27	Helical; Probable
	Transmembrane	281 – 298	18	Helical; Probable
	Transmembrane	313 – 336	24	Helical; Probable
	Topological domain	337 – 420	84	Cytoplasmic Probable
	Transmembrane	421 – 445	25	Helical; Probable
	Region	225 – 230	6	Strychnine-binding
Amino acid modifications
	Glycosylation	66	1	N-linked (GlcNAc...) Probable
	Disulfide bond	166 ↔ 180		By similarity
	Disulfide bond	226 ↔ 237		By similarity
Natural variations
	Alternative sequence	354 – 361	8	Missing in isoform b.		VSP_000081
Experimental info
	Sequence conflict	26	1	A → V in CAA68378. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform a (Long) [UniParc]. Last modified December 8, 2000. Version 4. Checksum: 0379C33A83C71FAA	FASTA	457	52,617
	10 20 30 40 50 60 MYSFNTLRFY LWETIVFFSL AASKEADAAR SAPKPMSPSD FLDKLMGRTS GYDARIRPNF 70 80 90 100 110 120 KGPPVNVSCN IFINSFGSIA ETTMDYRVNI FLRQQWNDPR LAYNEYPDDS LDLDPSMLDS 130 140 150 160 170 180 IWKPDLFFAN EKGAHFHEIT TDNKLLRISR NGNVLYSIRI TLTLACPMDL KNFPMDVQTC 190 200 210 220 230 240 IMQLESFGYT MNDLIFEWQE QGAVQVADGL TLPQFILKEE KDLRYCTKHY NTGKFTCIEA 250 260 270 280 290 300 RFHLERQMGY YLIQMYIPSL LIVILSWISF WINMDAAPAR VGLGITTVLT MTTQSSGSRA 310 320 330 340 350 360 SLPKVSYVKA IDIWMAVCLL FVFSALLEYA AVNFVSRQHK ELLRFRRKRR HHKSPMLNLF 370 380 390 400 410 420 QDDEGGEGRF NFSAYGMGPA CLQAKDGISV KGANNNNTTN PAPAPSKSPE EMRKLFIQRA 430 440 450 KKIDKISRIG FPMAFLIFNM FYWIIYKIVR REDVHNK
	Isoform b (Short) [UniParc]. Checksum: 3183E1304DA77B1E	FASTA	449	51,686
	10 20 30 40 50 60 MYSFNTLRFY LWETIVFFSL AASKEADAAR SAPKPMSPSD FLDKLMGRTS GYDARIRPNF 70 80 90 100 110 120 KGPPVNVSCN IFINSFGSIA ETTMDYRVNI FLRQQWNDPR LAYNEYPDDS LDLDPSMLDS 130 140 150 160 170 180 IWKPDLFFAN EKGAHFHEIT TDNKLLRISR NGNVLYSIRI TLTLACPMDL KNFPMDVQTC 190 200 210 220 230 240 IMQLESFGYT MNDLIFEWQE QGAVQVADGL TLPQFILKEE KDLRYCTKHY NTGKFTCIEA 250 260 270 280 290 300 RFHLERQMGY YLIQMYIPSL LIVILSWISF WINMDAAPAR VGLGITTVLT MTTQSSGSRA 310 320 330 340 350 360 SLPKVSYVKA IDIWMAVCLL FVFSALLEYA AVNFVSRQHK ELLRFRRKRR HHKDDEGGEG 370 380 390 400 410 420 RFNFSAYGMG PACLQAKDGI SVKGANNNNT TNPAPAPSKS PEEMRKLFIQ RAKKIDKISR 430 440 IGFPMAFLIF NMFYWIIYKI VRREDVHNK

References

[1]	"Functional properties of strychnine-sensitive glycine receptors expressed in Xenopus oocytes injected with a single mRNA." Akagi H., Hirai K., Hishinuma F. Neurosci. Res. 11:28-40(1991) [PubMed: 1716350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
[2]	"Alternative splicing generates two variants of the alpha 1 subunit of the inhibitory glycine receptor." Malosio M.L., Grenningloh G., Kuhse J., Schmieden V., Schmitt B., Prior P., Betz H. J. Biol. Chem. 266:2048-2053(1991) [PubMed: 1703526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), ALTERNATIVE SPLICING. Strain: Wistar. Tissue: Spinal cord.
[3]	"Openings of the rat recombinant alpha 1 homomeric glycine receptor as a function of the number of agonist molecules bound." Beato M., Groot-Kormelink P.J., Colquhoun D., Sivilotti L.G. J. Gen. Physiol. 119:443-466(2002) [PubMed: 11981023] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Strain: Sprague-Dawley. Tissue: Brain.
[4]	"Functional chloride channels by mammalian cell expression of rat glycine receptor subunit." Sontheimer H., Becker C.M., Pritchett D.B., Schofield P.R., Grenningloh G., Kettenmann H., Betz H., Seeburg P.H. Neuron 2:1491-1497(1989) [PubMed: 2483325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-30.
[5]	"The strychnine-binding subunit of the glycine receptor shows homology with nicotinic acetylcholine receptors." Grenningloh G., Rienitz A., Schmitt B., Methfessel C., Zensen M., Beyreuther K., Gundelfinger E.D., Betz H. Nature 328:215-220(1987) [PubMed: 3037383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 23-457 (ISOFORM B), PROTEIN SEQUENCE OF 29-49. Strain: Wistar.
[6]	"Localization of the strychnine binding site on the 48-kilodalton subunit of the glycine receptor." Ruiz-Gomez A., Morato E., Garcia-Calvo M., Valdivieso F., Mayor F. Jr. Biochemistry 29:7033-7040(1990) [PubMed: 2171639] [Abstract] Cited for: STRYCHNINE BINDING SITE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00833 mRNA. Translation: BAA00707.1. X55246 mRNA. Translation: CAA38987.1. M63915 mRNA. Translation: AAA63490.1. AJ310835 mRNA. Translation: CAC35979.1. Y00276 mRNA. Translation: CAA68378.1.
IPI	IPI00206855. IPI00324218.
PIR	JN0014. S20662.
RefSeq	NP_037265.1. NM_013133.1.
UniGene	Rn.10109.
3D structure databases
ProteinModelPortal	P07727.
SMR	P07727. Positions 277-337.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48879N.
STRING	P07727.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000063889; ENSRNOP00000063384; ENSRNOG00000013588.
GeneID	25674.
KEGG	rno:25674.
UCSC	NM_013133. rat.
Organism-specific databases
CTD	2741.
RGD	2704. Glra1.
Phylogenomic databases
eggNOG	maNOG06628.
HOVERGEN	HBG051707.
InParanoid	P07727.
OrthoDB	EOG4TF0KF.
Gene expression databases
ArrayExpress	P07727.
Genevestigator	P07727.
GermOnline	ENSRNOG00000013588. Rattus norvegicus.
Family and domain databases
InterPro	IPR006028. GABAA_rcpt. IPR008127. Glycine_rcpt_A. IPR008128. Glycine_rcpt_A1. IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
KO	K05193.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 2 hits. [Graphical view]
PRINTS	PR00253. GABAARECEPTR. PR01673. GLYRALPHA. PR01674. GLYRALPHA1. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607619.

Entry information

Entry name

GLRA1_RAT

Accession

Primary (citable) accession number: P07727
Secondary accession number(s): Q546L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	December 8, 2000
Last modified:	November 16, 2011
This is version 123 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program