Reviewed, UniProtKB/Swiss-Prot P07725 (CD8A_RAT)

Names and origin

Protein names

Recommended name:
    T-cell surface glycoprotein CD8 alpha chain
Alternative name(s):
    CD8 antigen 32 kDa chain
    OX-8 membrane antigen
    CD_antigen=CD8a

Gene names

Name:

Cd8a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Subunit structure	In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Immune response
Cellular component	Membrane
Domain	Immunoglobulin domain Signal Transmembrane
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	inflammatory response Non-traceable author statement. Source: RGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 236	210	T-cell surface glycoprotein CD8 alpha chain		PRO_0000014641
Regions
	Topological domain	27 – 189	163	Extracellular Potential
	Transmembrane	190 – 210	21	Potential
	Topological domain	211 – 236	26	Cytoplasmic Potential
	Domain	27 – 130	104	Ig-like V-type
Amino acid modifications
	Glycosylation	63	1	N-linked (GlcNAc...) Probable
	Glycosylation	144	1	O-linked (GalNAc...); partial Ref.3
	Glycosylation	148	1	O-linked (GalNAc...) Ref.3
	Glycosylation	152	1	O-linked (GalNAc...) Ref.3
	Glycosylation	158	1	O-linked (GalNAc...) Ref.3
	Glycosylation	160	1	O-linked (GalNAc...) Ref.3
	Disulfide bond	47 ↔ 119		Potential

Sequences

	Sequence		Length	Mass (Da)
	P07725-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: ADFAC54E4C99C1BE	FASTA	236	26,196
	10 20 30 40 50 60 MASRVICFLS LNLLLLDVIT RLQVSGQLQL SPKKVDAEIG QEVKLTCEVL RDTSQGCSWL 70 80 90 100 110 120 FRNSSSELLQ PTFIIYVSSS RSKLNDILDP NLFSARKENN KYILTLSKFS TKNQGYYFCS 130 140 150 160 170 180 ITSNSVMYFS PLVPVFQKVN SIITKPVTRA PTPVPPPTGT PRPLRPEACR PGASGSVEGM 190 200 210 220 230 GLGFACDIYI WAPLAGICAV LLLSLVITLI CCHRNRRRVC KCPRPLVKPR PSEKFV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes." Johnson P., Gagnon J., Barclay A.N., Williams A.F. EMBO J. 4:2539-2545(1985) [PubMed: 3932064] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
[3]	"Glycosylation sites identified by detection of glycosylated amino acids released from Edman degradation: the identification of Xaa-Pro-Xaa-Xaa as a motif for Thr-O-glycosylation." Gooley A.A., Classon B.J., Marschalek R., Williams K.L. Biochem. Biophys. Res. Commun. 178:1194-1200(1991) [PubMed: 1908233] [Abstract] Cited for: GLYCOSYLATION AT ASN-63; THR-144; THR-148; THR-152; THR-158 AND THR-160.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X03015 mRNA. Translation: CAA26798.1. BC088126 mRNA. Translation: AAH88126.1.
IPI	IPI00210541.
PIR	A24637.
RefSeq	NP_113726.1.
UniGene	Rn.10306
3D structure databases
HSSP	HSSP built from PDB template 1CD8 based on UniProtKB P01732.
ModBase	Search...
Protein-protein interaction databases
STRING	P07725.
Genome annotation databases
Ensembl	ENSRNOT00000009515; ENSRNOP00000009516; ENSRNOG00000007178; Rattus norvegicus. [Genome view]
GeneID	24930.
KEGG	rno:24930.
UCSC	NM_031538. rat.
Organism-specific databases
CTD	24930.
RGD	2316. Cd8a.
Phylogenomic databases
HOVERGEN	P07725.
Gene expression databases
ArrayExpress	P07725.
Genevestigator	P07725.
GermOnline	ENSRNOG00000007178. Rattus norvegicus.
Family and domain databases
InterPro	IPR015468. CD8_asu. IPR013151. Ig. IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003599. Ig_sub. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PANTHER	PTHR10441. CD8_alpha. 1 hit.
Pfam	PF00047. ig. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	604895.

Entry information

Entry name

CD8A_RAT

Accession

Primary (citable) accession number: P07725

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	October 13, 2009
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)