T-cell surface glycoprotein CD8 alpha chain precursor

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P07725 (CD8A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
T-cell surface glycoprotein CD8 alpha chain

Alternative name(s):
CD8 antigen 32 kDa chain
OX-8 membrane antigen
CD_antigen=CD8a

Gene names

Name:

Cd8a

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Subunit structure	In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Adaptive immunity Immunity
Cellular component	Membrane
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell activation Inferred from mutant phenotype PubMed 10458478. Source: RGD T cell mediated immunity Inferred from electronic annotation. Source: Compara cell surface receptor signaling pathway Inferred from electronic annotation. Source: Compara cytotoxic T cell differentiation Inferred from electronic annotation. Source: Compara defense response to virus Inferred from electronic annotation. Source: Compara inflammatory response Non-traceable author statement PubMed 11698471. Source: RGD positive regulation of calcium-mediated signaling Inferred from electronic annotation. Source: Compara
Cellular_component	external side of plasma membrane Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

Potential

Chain

27 – 236

210

T-cell surface glycoprotein CD8 alpha chain

PRO_0000014641

Regions

Topological domain

27 – 189

163

Extracellular Potential

Transmembrane

190 – 210

Helical; Potential

Topological domain

211 – 236

Cytoplasmic Potential

Domain

27 – 130

104

Ig-like V-type

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Probable

Glycosylation

144

O-linked (GalNAc...); partial Ref.3

Glycosylation

148

O-linked (GalNAc...) Ref.3

Glycosylation

152

O-linked (GalNAc...) Ref.3

Glycosylation

158

O-linked (GalNAc...) Ref.3

Glycosylation

160

O-linked (GalNAc...) Ref.3

Disulfide bond

47 ↔ 119

Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P07725 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: ADFAC54E4C99C1BE
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FASTA

236

26,196

        10         20         30         40         50         60 
MASRVICFLS LNLLLLDVIT RLQVSGQLQL SPKKVDAEIG QEVKLTCEVL RDTSQGCSWL 

        70         80         90        100        110        120 
FRNSSSELLQ PTFIIYVSSS RSKLNDILDP NLFSARKENN KYILTLSKFS TKNQGYYFCS 

       130        140        150        160        170        180 
ITSNSVMYFS PLVPVFQKVN SIITKPVTRA PTPVPPPTGT PRPLRPEACR PGASGSVEGM 

       190        200        210        220        230 
GLGFACDIYI WAPLAGICAV LLLSLVITLI CCHRNRRRVC KCPRPLVKPR PSEKFV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes." Johnson P., Gagnon J., Barclay A.N., Williams A.F. EMBO J. 4:2539-2545(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
[3]	"Glycosylation sites identified by detection of glycosylated amino acids released from Edman degradation: the identification of Xaa-Pro-Xaa-Xaa as a motif for Thr-O-glycosylation." Gooley A.A., Classon B.J., Marschalek R., Williams K.L. Biochem. Biophys. Res. Commun. 178:1194-1200(1991) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-63; THR-144; THR-148; THR-152; THR-158 AND THR-160.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03015 mRNA. Translation: CAA26798.1. BC088126 mRNA. Translation: AAH88126.1.
IPI	IPI00210541.
PIR	A24637.
RefSeq	NP_113726.1. NM_031538.2.
UniGene	Rn.10306.
3D structure databases
ProteinModelPortal	P07725.
SMR	P07725. Positions 27-140.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000009516.
PTM databases
PhosphoSite	P07725.
Proteomic databases
PRIDE	P07725.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000009515; ENSRNOP00000009516; ENSRNOG00000007178.
GeneID	24930.
KEGG	rno:24930.
UCSC	RGD:2316. rat.
Organism-specific databases
CTD	925.
RGD	2316. Cd8a.
Phylogenomic databases
eggNOG	NOG46810.
GeneTree	ENSGT00510000048935.
HOGENOM	HOG000004794.
HOVERGEN	HBG008488.
InParanoid	P07725.
KO	K06458.
OrthoDB	EOG4JDH84.
Gene expression databases
Genevestigator	P07725.
GermOnline	ENSRNOG00000007178. Rattus norvegicus.
Family and domain databases
Gene3D	2.60.40.10. 1 hit.
InterPro	IPR015468. CD8_asu. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013151. Immunoglobulin. [Graphical view]
PANTHER	PTHR10441. PTHR10441. 1 hit.
Pfam	PF00047. ig. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	604895.

Entry information

Entry name

CD8A_RAT

Accession

Primary (citable) accession number: P07725

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	April 3, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents