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Protein

T-cell surface glycoprotein CD8 alpha chain

Gene

Cd8a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: Ensembl
  • protein kinase binding Source: RGD

GO - Biological processi

  • cell surface receptor signaling pathway Source: GO_Central
  • cytotoxic T cell differentiation Source: GO_Central
  • defense response to virus Source: Ensembl
  • inflammatory response Source: RGD
  • positive regulation of calcium-mediated signaling Source: Ensembl
  • T cell activation Source: RGD
  • T cell mediated immunity Source: GO_Central

Keywordsi

Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-RNO-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD8 alpha chain
Alternative name(s):
CD8 antigen 32 kDa chain
OX-8 membrane antigen
CD_antigen: CD8a
Gene namesi
Name:Cd8a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2316. Cd8a.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini27 – 189ExtracellularSequence analysisAdd BLAST163
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Topological domaini211 – 236CytoplasmicSequence analysisAdd BLAST26

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000001464127 – 236T-cell surface glycoprotein CD8 alpha chainAdd BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 119PROSITE-ProRule annotation
Glycosylationi63N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi144O-linked (GalNAc...) threonine; partial1 Publication1
Glycosylationi148O-linked (GalNAc...) threonine1 Publication1
Glycosylationi152O-linked (GalNAc...) threonine1 Publication1
Glycosylationi158O-linked (GalNAc...) threonine1 Publication1
Glycosylationi160O-linked (GalNAc...) threonine1 Publication1
Lipidationi211S-palmitoyl cysteineBy similarity1

Post-translational modificationi

Palmitoylated, but association with CD8B seems to be more important for the enrichment of CD8A in lipid rafts.By similarity
O-glycosylated.By similarity
Phosphorylated in cytotoxic T-lymphocytes (CTLs) following activation.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP07725.
PRIDEiP07725.

PTM databases

PhosphoSitePlusiP07725.

Expressioni

Gene expression databases

BgeeiENSRNOG00000007178.
GenevisibleiP07725. RN.

Interactioni

Subunit structurei

Forms disulfide-linked heterodimers with CD8B at the cell surface. Forms also homodimers in several cell types including NK-cells or peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner.By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: Ensembl
  • protein kinase binding Source: RGD

Protein-protein interaction databases

DIPiDIP-60779N.
STRINGi10116.ENSRNOP00000009516.

Structurei

3D structure databases

ProteinModelPortaliP07725.
SMRiP07725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 130Ig-like V-typeAdd BLAST104

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IWN3. Eukaryota.
ENOG410Z6EI. LUCA.
GeneTreeiENSGT00510000048935.
HOGENOMiHOG000004794.
HOVERGENiHBG008488.
InParanoidiP07725.
KOiK06458.
OMAiRVCKCPR.
OrthoDBiEOG091G0YIT.
PhylomeDBiP07725.
TreeFamiTF336070.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR015468. CD8_asu.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
PANTHERiPTHR10441. PTHR10441. 1 hit.
PfamiView protein in Pfam
PF07686. V-set. 1 hit.
SMARTiView protein in SMART
SM00409. IG. 1 hit.
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASRVICFLS LNLLLLDVIT RLQVSGQLQL SPKKVDAEIG QEVKLTCEVL
60 70 80 90 100
RDTSQGCSWL FRNSSSELLQ PTFIIYVSSS RSKLNDILDP NLFSARKENN
110 120 130 140 150
KYILTLSKFS TKNQGYYFCS ITSNSVMYFS PLVPVFQKVN SIITKPVTRA
160 170 180 190 200
PTPVPPPTGT PRPLRPEACR PGASGSVEGM GLGFACDIYI WAPLAGICAV
210 220 230
LLLSLVITLI CCHRNRRRVC KCPRPLVKPR PSEKFV
Length:236
Mass (Da):26,196
Last modified:April 1, 1988 - v1
Checksum:iADFAC54E4C99C1BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03015 mRNA. Translation: CAA26798.1.
BC088126 mRNA. Translation: AAH88126.1.
PIRiA24637.
RefSeqiNP_113726.1. NM_031538.2.
XP_006236692.1. XM_006236630.3.
XP_008761198.1. XM_008762976.2.
UniGeneiRn.10306.

Genome annotation databases

EnsembliENSRNOT00000009515; ENSRNOP00000009516; ENSRNOG00000007178.
GeneIDi24930.
KEGGirno:24930.
UCSCiRGD:2316. rat.

Similar proteinsi

Entry informationi

Entry nameiCD8A_RAT
AccessioniPrimary (citable) accession number: P07725
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: September 27, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome