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Protein

Myelin-associated glycoprotein

Gene

Mag

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Sialic acidBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. protein kinase binding Source: RGD
  3. receptor binding Source: RGD

GO - Biological processi

  1. cell adhesion Source: UniProtKB-KW
  2. cellular response to mechanical stimulus Source: RGD
  3. negative regulation of neuron differentiation Source: RGD
  4. negative regulation of neuron projection development Source: RGD
  5. nervous system development Source: RGD
  6. positive regulation of astrocyte differentiation Source: RGD
  7. substantia nigra development Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Lectin

Enzyme and pathway databases

ReactomeiREACT_296486. Axonal growth inhibition (RHOA activation).
REACT_336327. Basigin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Myelin-associated glycoprotein
Alternative name(s):
Brain neuron cytoplasmic protein 3
Sialic acid-binding Ig-like lectin 4a
Short name:
Siglec-4a
Gene namesi
Name:Mag
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3035. Mag.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 516497ExtracellularSequence AnalysisAdd
BLAST
Transmembranei517 – 53620HelicalSequence AnalysisAdd
BLAST
Topological domaini537 – 62690CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
  3. mesaxon Source: RGD
  4. myelin sheath adaxonal region Source: RGD
  5. paranode region of axon Source: Ensembl
  6. plasma membrane Source: RGD
  7. Schmidt-Lanterman incisure Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 626607Myelin-associated glycoproteinPRO_0000014858Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi37 ↔ 1651 Publication
Disulfide bondi42 ↔ 1001 Publication
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi159 ↔ 2171 Publication
Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi261 ↔ 3051 Publication
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi332 – 3321N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi347 ↔ 3921 Publication
Glycosylationi406 – 4061N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi421 ↔ 4301 Publication
Disulfide bondi432 ↔ 4881 Publication
Glycosylationi450 – 4501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi454 – 4541N-linked (GlcNAc...)Sequence Analysis
Lipidationi531 – 5311S-palmitoyl cysteine1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP07722.
PRIDEiP07722.

PTM databases

PhosphoSiteiP07722.

Expressioni

Gene expression databases

ExpressionAtlasiP07722. baseline and differential.
GenevestigatoriP07722.

Interactioni

Subunit structurei

Binds to RTN4R.By similarity

Protein-protein interaction databases

BioGridi248059. 1 interaction.
MINTiMINT-237515.

Structurei

3D structure databases

ProteinModelPortaliP07722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 12099Ig-like V-typeAdd
BLAST
Domaini139 – 23799Ig-like C2-type 1Add
BLAST
Domaini241 – 32585Ig-like C2-type 2Add
BLAST
Domaini327 – 41286Ig-like C2-type 3Add
BLAST
Domaini413 – 50896Ig-like C2-type 4Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG290903.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000113464.
HOVERGENiHBG006317.
InParanoidiP07722.
KOiK06771.
OMAiCQASFPN.
PhylomeDBiP07722.
TreeFamiTF332441.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform L-MAG (identifier: P07722-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIFLTTLPLF WIMISASRGG HWGAWMPSSI SAFEGTCVSI PCRFDFPDEL
60 70 80 90 100
RPAVVHGVWY FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC
110 120 130 140 150
TLLLSTLSPE LGGKYYFRGD LGGYNQYTFS EHSVLDIINT PNIVVPPEVV
160 170 180 190 200
AGTEVEVSCM VPDNCPELRP ELSWLGHEGL GEPTVLGRLR EDEGTWVQVS
210 220 230 240 250
LLHFVPTREA NGHRLGCQAA FPNTTLQFEG YASLDVKYPP VIVEMNSSVE
260 270 280 290 300
AIEGSHVSLL CGADSNPPPL LTWMRDGMVL REAVAESLYL DLEEVTPAED
310 320 330 340 350
GIYACLAENA YGQDNRTVEL SVMYAPWKPT VNGTVVAVEG ETVSILCSTQ
360 370 380 390 400
SNPDPILTIF KEKQILATVI YESQLQLELP AVTPEDDGEY WCVAENQYGQ
410 420 430 440 450
RATAFNLSVE FAPIILLESH CAAARDTVQC LCVVKSNPEP SVAFELPSRN
460 470 480 490 500
VTVNETEREF VYSERSGLLL TSILTLRGQA QAPPRVICTS RNLYGTQSLE
510 520 530 540 550
LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK NVTESPSFSA
560 570 580 590 600
GDNPHVLYSP EFRISGAPDK YESEKRLGSE RRLLGLRGEP PELDLSYSHS
610 620
DLGKRPTKDS YTLTEELAEY AEIRVK
Length:626
Mass (Da):69,353
Last modified:April 1, 1988 - v1
Checksum:iE97998E280ECD635
GO
Isoform S-MAG (identifier: P07722-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     574-582: EKRLGSERR → REVSTRDCH
     583-626: Missing.

Show »
Length:582
Mass (Da):64,351
Checksum:i2A7A9A9FF76D48C4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti309 – 3102NA → KS (PubMed:6586369).Curated
Sequence conflicti309 – 3102NA → KS (PubMed:4020419).Curated
Sequence conflicti309 – 3102NA → KS (PubMed:6347394).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei574 – 5829EKRLGSERR → REVSTRDCH in isoform S-MAG. 1 PublicationVSP_002529
Alternative sequencei583 – 62644Missing in isoform S-MAG. 1 PublicationVSP_002530Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14871 mRNA. Translation: AAA41556.1.
M22357 mRNA. Translation: AAA41558.1.
M16800 mRNA. Translation: AAA41557.1.
X05301 mRNA. Translation: CAA28920.1.
M11721 mRNA. Translation: AAA42082.1.
M36702 mRNA. Translation: AAA40831.1.
V01544 Genomic DNA. Translation: CAA24786.1.
PIRiA29028. BNRT3.
RefSeqiNP_058886.1. NM_017190.4. [P07722-1]
XP_006228886.1. XM_006228824.2. [P07722-1]
XP_006228887.1. XM_006228825.2. [P07722-1]
XP_008757359.1. XM_008759137.1. [P07722-1]
XP_008757360.1. XM_008759138.1. [P07722-1]
UniGeneiRn.87331.

Genome annotation databases

EnsembliENSRNOT00000028544; ENSRNOP00000028544; ENSRNOG00000021023. [P07722-1]
GeneIDi29409.
KEGGirno:29409.
UCSCiRGD:3035. rat. [P07722-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14871 mRNA. Translation: AAA41556.1.
M22357 mRNA. Translation: AAA41558.1.
M16800 mRNA. Translation: AAA41557.1.
X05301 mRNA. Translation: CAA28920.1.
M11721 mRNA. Translation: AAA42082.1.
M36702 mRNA. Translation: AAA40831.1.
V01544 Genomic DNA. Translation: CAA24786.1.
PIRiA29028. BNRT3.
RefSeqiNP_058886.1. NM_017190.4. [P07722-1]
XP_006228886.1. XM_006228824.2. [P07722-1]
XP_006228887.1. XM_006228825.2. [P07722-1]
XP_008757359.1. XM_008759137.1. [P07722-1]
XP_008757360.1. XM_008759138.1. [P07722-1]
UniGeneiRn.87331.

3D structure databases

ProteinModelPortaliP07722.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248059. 1 interaction.
MINTiMINT-237515.

PTM databases

PhosphoSiteiP07722.

Proteomic databases

PaxDbiP07722.
PRIDEiP07722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028544; ENSRNOP00000028544; ENSRNOG00000021023. [P07722-1]
GeneIDi29409.
KEGGirno:29409.
UCSCiRGD:3035. rat. [P07722-1]

Organism-specific databases

CTDi4099.
RGDi3035. Mag.

Phylogenomic databases

eggNOGiNOG290903.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000113464.
HOVERGENiHBG006317.
InParanoidiP07722.
KOiK06771.
OMAiCQASFPN.
PhylomeDBiP07722.
TreeFamiTF332441.

Enzyme and pathway databases

ReactomeiREACT_296486. Axonal growth inhibition (RHOA activation).
REACT_336327. Basigin interactions.

Miscellaneous databases

NextBioi609068.
PROiP07722.

Gene expression databases

ExpressionAtlasiP07722. baseline and differential.
GenevestigatoriP07722.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Two forms of 1B236/myelin-associated glycoprotein, a cell adhesion molecule for postnatal neural development, are produced by alternative splicing."
    Lai C., Brow M.A., Nave K.-A., Noronha A.B., Quarles R.H., Bloom F.E., Milner R.J., Sutcliffe J.G.
    Proc. Natl. Acad. Sci. U.S.A. 84:4337-4341(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L-MAG AND S-MAG).
  2. "Molecular cloning and primary structure of myelin-associated glycoprotein."
    Arquint M., Roder J., Chia L.S., Down J., Wilkinson D., Bayley H., Braun P., Dunn R.
    Proc. Natl. Acad. Sci. U.S.A. 84:600-604(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM L-MAG).
  3. "The amino acid sequences of the myelin-associated glycoproteins: homology to the immunoglobulin gene superfamily."
    Salzer J.L., Holmes W.P., Colman D.R.
    J. Cell Biol. 104:957-965(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM L-MAG).
  4. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 76-98; 459-477 AND 492-508, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  5. "Cellular localization and function of the proteins encoded by brain-specific mRNAs."
    Sutcliffe J.G., Milner R.J., Bloom F.E.
    Cold Spring Harb. Symp. Quant. Biol. 48:477-484(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 309-626.
  6. "Immunocytochemical mapping of 1B236, a brain-specific neuronal polypeptide deduced from the sequence of a cloned mRNA."
    Bloom F.E., Battenberg E.L.F., Milner R.J., Sutcliffe J.G.
    J. Neurosci. 5:1781-1802(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 309-626.
    Tissue: Brain.
  7. "Identifying the protein products of brain-specific genes with antibodies to chemically synthesized peptides."
    Sutcliffe J.G., Milner R.J., Shinnick T.M., Bloom F.E.
    Cell 33:671-682(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 309-626.
  8. "The myelin-associated glycoproteins: membrane disposition, evidence of a novel disulfide linkage between immunoglobulin-like domains, and posttranslational palmitylation."
    Pedraza L., Owens G.C., Green L.A.D., Salzer J.L.
    J. Cell Biol. 111:2651-2661(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS, PALMITOYLATION AT CYS-531, PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiMAG_RAT
AccessioniPrimary (citable) accession number: P07722
Secondary accession number(s): P02685, P07723
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: April 1, 2015
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.