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P07722 (MAG_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myelin-associated glycoprotein
Alternative name(s):
Brain neuron cytoplasmic protein 3
Sialic acid-binding Ig-like lectin 4a
Short name=Siglec-4a
Gene names
Name:Mag
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length626 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid By similarity.

Subunit structure

Binds to RTN4R By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Contains 4 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform L-MAG (identifier: P07722-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform S-MAG (identifier: P07722-2)

The sequence of this isoform differs from the canonical sequence as follows:
     574-582: EKRLGSERR → REVSTRDCH
     583-626: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919
Chain20 – 626607Myelin-associated glycoprotein
PRO_0000014858

Regions

Topological domain20 – 516497Extracellular Potential
Transmembrane517 – 53620Helical; Potential
Topological domain537 – 62690Cytoplasmic Potential
Domain22 – 12099Ig-like V-type
Domain139 – 23799Ig-like C2-type 1
Domain241 – 32585Ig-like C2-type 2
Domain327 – 41286Ig-like C2-type 3
Domain413 – 50896Ig-like C2-type 4

Sites

Binding site1181Sialic acid By similarity

Amino acid modifications

Lipidation5311S-palmitoyl cysteine Ref.8
Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation2231N-linked (GlcNAc...) Potential
Glycosylation2461N-linked (GlcNAc...) Potential
Glycosylation3151N-linked (GlcNAc...) Potential
Glycosylation3321N-linked (GlcNAc...) Potential
Glycosylation4061N-linked (GlcNAc...) Potential
Glycosylation4501N-linked (GlcNAc...) Potential
Glycosylation4541N-linked (GlcNAc...) Potential
Disulfide bond37 ↔ 165 Probable
Disulfide bond42 ↔ 100 Probable
Disulfide bond159 ↔ 217 Probable
Disulfide bond261 ↔ 305 Probable
Disulfide bond347 ↔ 392 Probable
Disulfide bond421 ↔ 430 Probable
Disulfide bond432 ↔ 488 Probable

Natural variations

Alternative sequence574 – 5829EKRLGSERR → REVSTRDCH in isoform S-MAG.
VSP_002529
Alternative sequence583 – 62644Missing in isoform S-MAG.
VSP_002530

Experimental info

Sequence conflict309 – 3102NA → KS Ref.5
Sequence conflict309 – 3102NA → KS Ref.6
Sequence conflict309 – 3102NA → KS Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform L-MAG [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: E97998E280ECD635

FASTA62669,353
        10         20         30         40         50         60 
MIFLTTLPLF WIMISASRGG HWGAWMPSSI SAFEGTCVSI PCRFDFPDEL RPAVVHGVWY 

        70         80         90        100        110        120 
FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC TLLLSTLSPE LGGKYYFRGD 

       130        140        150        160        170        180 
LGGYNQYTFS EHSVLDIINT PNIVVPPEVV AGTEVEVSCM VPDNCPELRP ELSWLGHEGL 

       190        200        210        220        230        240 
GEPTVLGRLR EDEGTWVQVS LLHFVPTREA NGHRLGCQAA FPNTTLQFEG YASLDVKYPP 

       250        260        270        280        290        300 
VIVEMNSSVE AIEGSHVSLL CGADSNPPPL LTWMRDGMVL REAVAESLYL DLEEVTPAED 

       310        320        330        340        350        360 
GIYACLAENA YGQDNRTVEL SVMYAPWKPT VNGTVVAVEG ETVSILCSTQ SNPDPILTIF 

       370        380        390        400        410        420 
KEKQILATVI YESQLQLELP AVTPEDDGEY WCVAENQYGQ RATAFNLSVE FAPIILLESH 

       430        440        450        460        470        480 
CAAARDTVQC LCVVKSNPEP SVAFELPSRN VTVNETEREF VYSERSGLLL TSILTLRGQA 

       490        500        510        520        530        540 
QAPPRVICTS RNLYGTQSLE LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK 

       550        560        570        580        590        600 
NVTESPSFSA GDNPHVLYSP EFRISGAPDK YESEKRLGSE RRLLGLRGEP PELDLSYSHS 

       610        620 
DLGKRPTKDS YTLTEELAEY AEIRVK 

« Hide

Isoform S-MAG [UniParc].

Checksum: 2A7A9A9FF76D48C4
Show »

FASTA58264,351

References

[1]"Two forms of 1B236/myelin-associated glycoprotein, a cell adhesion molecule for postnatal neural development, are produced by alternative splicing."
Lai C., Brow M.A., Nave K.-A., Noronha A.B., Quarles R.H., Bloom F.E., Milner R.J., Sutcliffe J.G.
Proc. Natl. Acad. Sci. U.S.A. 84:4337-4341(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L-MAG AND S-MAG).
[2]"Molecular cloning and primary structure of myelin-associated glycoprotein."
Arquint M., Roder J., Chia L.S., Down J., Wilkinson D., Bayley H., Braun P., Dunn R.
Proc. Natl. Acad. Sci. U.S.A. 84:600-604(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM L-MAG).
[3]"The amino acid sequences of the myelin-associated glycoproteins: homology to the immunoglobulin gene superfamily."
Salzer J.L., Holmes W.P., Colman D.R.
J. Cell Biol. 104:957-965(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM L-MAG).
[4]Lubec G., Kang S.U., Lubec S.
Submitted (SEP-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 76-98; 459-477 AND 492-508, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
[5]"Cellular localization and function of the proteins encoded by brain-specific mRNAs."
Sutcliffe J.G., Milner R.J., Bloom F.E.
Cold Spring Harb. Symp. Quant. Biol. 48:477-484(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 309-626.
[6]"Immunocytochemical mapping of 1B236, a brain-specific neuronal polypeptide deduced from the sequence of a cloned mRNA."
Bloom F.E., Battenberg E.L.F., Milner R.J., Sutcliffe J.G.
J. Neurosci. 5:1781-1802(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 309-626.
Tissue: Brain.
[7]"Identifying the protein products of brain-specific genes with antibodies to chemically synthesized peptides."
Sutcliffe J.G., Milner R.J., Shinnick T.M., Bloom F.E.
Cell 33:671-682(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 309-626.
[8]"The myelin-associated glycoproteins: membrane disposition, evidence of a novel disulfide linkage between immunoglobulin-like domains, and posttranslational palmitylation."
Pedraza L., Owens G.C., Green L.A.D., Salzer J.L.
J. Cell Biol. 111:2651-2661(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS, PALMITOYLATION AT CYS-531, PARTIAL PROTEIN SEQUENCE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M14871 mRNA. Translation: AAA41556.1.
M22357 mRNA. Translation: AAA41558.1.
M16800 mRNA. Translation: AAA41557.1.
X05301 mRNA. Translation: CAA28920.1.
M11721 mRNA. Translation: AAA42082.1.
M36702 mRNA. Translation: AAA40831.1.
V01544 Genomic DNA. Translation: CAA24786.1.
PIRBNRT3. A29028.
RefSeqNP_058886.1. NM_017190.4. [P07722-1]
XP_006228885.1. XM_006228823.1. [P07722-1]
XP_006228886.1. XM_006228824.1. [P07722-1]
XP_006228887.1. XM_006228825.1. [P07722-1]
XP_006228888.1. XM_006228826.1. [P07722-1]
UniGeneRn.87331.

3D structure databases

ProteinModelPortalP07722.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248059. 1 interaction.
MINTMINT-237515.

PTM databases

PhosphoSiteP07722.

Proteomic databases

PaxDbP07722.
PRIDEP07722.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000028544; ENSRNOP00000028544; ENSRNOG00000021023. [P07722-1]
GeneID29409.
KEGGrno:29409.
UCSCRGD:3035. rat. [P07722-1]

Organism-specific databases

CTD4099.
RGD3035. Mag.

Phylogenomic databases

eggNOGNOG290903.
GeneTreeENSGT00720000108720.
HOGENOMHOG000113464.
HOVERGENHBG006317.
InParanoidP07722.
KOK06771.
OMACQASFPN.
PhylomeDBP07722.
TreeFamTF332441.

Gene expression databases

ArrayExpressP07722.
GenevestigatorP07722.

Family and domain databases

Gene3D2.60.40.10. 4 hits.
InterProIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamPF08205. C2-set_2. 1 hit.
PF07679. I-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
PROSITEPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio609068.
PROP07722.

Entry information

Entry nameMAG_RAT
AccessionPrimary (citable) accession number: P07722
Secondary accession number(s): P02685, P07723
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: May 14, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families