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P07722

- MAG_RAT

UniProt

P07722 - MAG_RAT

Protein

Myelin-associated glycoprotein

Gene

Mag

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Sialic acidBy similarity

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Lectin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myelin-associated glycoprotein
    Alternative name(s):
    Brain neuron cytoplasmic protein 3
    Sialic acid-binding Ig-like lectin 4a
    Short name:
    Siglec-4a
    Gene namesi
    Name:Mag
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi3035. Mag.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. paranode region of axon Source: Ensembl
    3. Schmidt-Lanterman incisure Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Add
    BLAST
    Chaini20 – 626607Myelin-associated glycoproteinPRO_0000014858Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi37 ↔ 1651 Publication
    Disulfide bondi42 ↔ 1001 Publication
    Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi159 ↔ 2171 Publication
    Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi261 ↔ 3051 Publication
    Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi332 – 3321N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi347 ↔ 3921 Publication
    Glycosylationi406 – 4061N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi421 ↔ 4301 Publication
    Disulfide bondi432 ↔ 4881 Publication
    Glycosylationi450 – 4501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi454 – 4541N-linked (GlcNAc...)Sequence Analysis
    Lipidationi531 – 5311S-palmitoyl cysteine1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP07722.
    PRIDEiP07722.

    PTM databases

    PhosphoSiteiP07722.

    Expressioni

    Gene expression databases

    ArrayExpressiP07722.
    GenevestigatoriP07722.

    Interactioni

    Subunit structurei

    Binds to RTN4R.By similarity

    Protein-protein interaction databases

    BioGridi248059. 1 interaction.
    MINTiMINT-237515.

    Structurei

    3D structure databases

    ProteinModelPortaliP07722.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 516497ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini537 – 62690CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei517 – 53620HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 12099Ig-like V-typeAdd
    BLAST
    Domaini139 – 23799Ig-like C2-type 1Add
    BLAST
    Domaini241 – 32585Ig-like C2-type 2Add
    BLAST
    Domaini327 – 41286Ig-like C2-type 3Add
    BLAST
    Domaini413 – 50896Ig-like C2-type 4Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG290903.
    GeneTreeiENSGT00720000108720.
    HOGENOMiHOG000113464.
    HOVERGENiHBG006317.
    InParanoidiP07722.
    KOiK06771.
    OMAiCQASFPN.
    PhylomeDBiP07722.
    TreeFamiTF332441.

    Family and domain databases

    Gene3Di2.60.40.10. 4 hits.
    InterProiIPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR013098. Ig_I-set.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    [Graphical view]
    PfamiPF08205. C2-set_2. 1 hit.
    PF07679. I-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 2 hits.
    SM00408. IGc2. 2 hits.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform L-MAG (identifier: P07722-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MIFLTTLPLF WIMISASRGG HWGAWMPSSI SAFEGTCVSI PCRFDFPDEL    50
    RPAVVHGVWY FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC 100
    TLLLSTLSPE LGGKYYFRGD LGGYNQYTFS EHSVLDIINT PNIVVPPEVV 150
    AGTEVEVSCM VPDNCPELRP ELSWLGHEGL GEPTVLGRLR EDEGTWVQVS 200
    LLHFVPTREA NGHRLGCQAA FPNTTLQFEG YASLDVKYPP VIVEMNSSVE 250
    AIEGSHVSLL CGADSNPPPL LTWMRDGMVL REAVAESLYL DLEEVTPAED 300
    GIYACLAENA YGQDNRTVEL SVMYAPWKPT VNGTVVAVEG ETVSILCSTQ 350
    SNPDPILTIF KEKQILATVI YESQLQLELP AVTPEDDGEY WCVAENQYGQ 400
    RATAFNLSVE FAPIILLESH CAAARDTVQC LCVVKSNPEP SVAFELPSRN 450
    VTVNETEREF VYSERSGLLL TSILTLRGQA QAPPRVICTS RNLYGTQSLE 500
    LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK NVTESPSFSA 550
    GDNPHVLYSP EFRISGAPDK YESEKRLGSE RRLLGLRGEP PELDLSYSHS 600
    DLGKRPTKDS YTLTEELAEY AEIRVK 626
    Length:626
    Mass (Da):69,353
    Last modified:April 1, 1988 - v1
    Checksum:iE97998E280ECD635
    GO
    Isoform S-MAG (identifier: P07722-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         574-582: EKRLGSERR → REVSTRDCH
         583-626: Missing.

    Show »
    Length:582
    Mass (Da):64,351
    Checksum:i2A7A9A9FF76D48C4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti309 – 3102NA → KS(PubMed:6586369)Curated
    Sequence conflicti309 – 3102NA → KS(PubMed:4020419)Curated
    Sequence conflicti309 – 3102NA → KS(PubMed:6347394)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei574 – 5829EKRLGSERR → REVSTRDCH in isoform S-MAG. 1 PublicationVSP_002529
    Alternative sequencei583 – 62644Missing in isoform S-MAG. 1 PublicationVSP_002530Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14871 mRNA. Translation: AAA41556.1.
    M22357 mRNA. Translation: AAA41558.1.
    M16800 mRNA. Translation: AAA41557.1.
    X05301 mRNA. Translation: CAA28920.1.
    M11721 mRNA. Translation: AAA42082.1.
    M36702 mRNA. Translation: AAA40831.1.
    V01544 Genomic DNA. Translation: CAA24786.1.
    PIRiA29028. BNRT3.
    RefSeqiNP_058886.1. NM_017190.4. [P07722-1]
    XP_006228885.1. XM_006228823.1. [P07722-1]
    XP_006228886.1. XM_006228824.1. [P07722-1]
    XP_006228887.1. XM_006228825.1. [P07722-1]
    XP_006228888.1. XM_006228826.1. [P07722-1]
    UniGeneiRn.87331.

    Genome annotation databases

    EnsembliENSRNOT00000028544; ENSRNOP00000028544; ENSRNOG00000021023. [P07722-1]
    GeneIDi29409.
    KEGGirno:29409.
    UCSCiRGD:3035. rat. [P07722-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14871 mRNA. Translation: AAA41556.1 .
    M22357 mRNA. Translation: AAA41558.1 .
    M16800 mRNA. Translation: AAA41557.1 .
    X05301 mRNA. Translation: CAA28920.1 .
    M11721 mRNA. Translation: AAA42082.1 .
    M36702 mRNA. Translation: AAA40831.1 .
    V01544 Genomic DNA. Translation: CAA24786.1 .
    PIRi A29028. BNRT3.
    RefSeqi NP_058886.1. NM_017190.4. [P07722-1 ]
    XP_006228885.1. XM_006228823.1. [P07722-1 ]
    XP_006228886.1. XM_006228824.1. [P07722-1 ]
    XP_006228887.1. XM_006228825.1. [P07722-1 ]
    XP_006228888.1. XM_006228826.1. [P07722-1 ]
    UniGenei Rn.87331.

    3D structure databases

    ProteinModelPortali P07722.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248059. 1 interaction.
    MINTi MINT-237515.

    PTM databases

    PhosphoSitei P07722.

    Proteomic databases

    PaxDbi P07722.
    PRIDEi P07722.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000028544 ; ENSRNOP00000028544 ; ENSRNOG00000021023 . [P07722-1 ]
    GeneIDi 29409.
    KEGGi rno:29409.
    UCSCi RGD:3035. rat. [P07722-1 ]

    Organism-specific databases

    CTDi 4099.
    RGDi 3035. Mag.

    Phylogenomic databases

    eggNOGi NOG290903.
    GeneTreei ENSGT00720000108720.
    HOGENOMi HOG000113464.
    HOVERGENi HBG006317.
    InParanoidi P07722.
    KOi K06771.
    OMAi CQASFPN.
    PhylomeDBi P07722.
    TreeFami TF332441.

    Miscellaneous databases

    NextBioi 609068.
    PROi P07722.

    Gene expression databases

    ArrayExpressi P07722.
    Genevestigatori P07722.

    Family and domain databases

    Gene3Di 2.60.40.10. 4 hits.
    InterProi IPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR013098. Ig_I-set.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    [Graphical view ]
    Pfami PF08205. C2-set_2. 1 hit.
    PF07679. I-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 2 hits.
    SM00408. IGc2. 2 hits.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two forms of 1B236/myelin-associated glycoprotein, a cell adhesion molecule for postnatal neural development, are produced by alternative splicing."
      Lai C., Brow M.A., Nave K.-A., Noronha A.B., Quarles R.H., Bloom F.E., Milner R.J., Sutcliffe J.G.
      Proc. Natl. Acad. Sci. U.S.A. 84:4337-4341(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L-MAG AND S-MAG).
    2. "Molecular cloning and primary structure of myelin-associated glycoprotein."
      Arquint M., Roder J., Chia L.S., Down J., Wilkinson D., Bayley H., Braun P., Dunn R.
      Proc. Natl. Acad. Sci. U.S.A. 84:600-604(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM L-MAG).
    3. "The amino acid sequences of the myelin-associated glycoproteins: homology to the immunoglobulin gene superfamily."
      Salzer J.L., Holmes W.P., Colman D.R.
      J. Cell Biol. 104:957-965(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM L-MAG).
    4. Lubec G., Kang S.U., Lubec S.
      Submitted (SEP-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 76-98; 459-477 AND 492-508, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    5. "Cellular localization and function of the proteins encoded by brain-specific mRNAs."
      Sutcliffe J.G., Milner R.J., Bloom F.E.
      Cold Spring Harb. Symp. Quant. Biol. 48:477-484(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 309-626.
    6. "Immunocytochemical mapping of 1B236, a brain-specific neuronal polypeptide deduced from the sequence of a cloned mRNA."
      Bloom F.E., Battenberg E.L.F., Milner R.J., Sutcliffe J.G.
      J. Neurosci. 5:1781-1802(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 309-626.
      Tissue: Brain.
    7. "Identifying the protein products of brain-specific genes with antibodies to chemically synthesized peptides."
      Sutcliffe J.G., Milner R.J., Shinnick T.M., Bloom F.E.
      Cell 33:671-682(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 309-626.
    8. "The myelin-associated glycoproteins: membrane disposition, evidence of a novel disulfide linkage between immunoglobulin-like domains, and posttranslational palmitylation."
      Pedraza L., Owens G.C., Green L.A.D., Salzer J.L.
      J. Cell Biol. 111:2651-2661(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS, PALMITOYLATION AT CYS-531, PARTIAL PROTEIN SEQUENCE.

    Entry informationi

    Entry nameiMAG_RAT
    AccessioniPrimary (citable) accession number: P07722
    Secondary accession number(s): P02685, P07723
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3