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Protein

DNA ligase

Gene

1.3

Organism
Enterobacteria phage T3 (Bacteriophage T3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase, which is expressed in the early stage of lytic development, has been implicated in T7 DNA synthesis and genetic recombination. It may also play a role in T7 DNA repair.

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.PROSITE-ProRule annotation

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei34N6-AMP-lysine intermediatePROSITE-ProRule annotation1
Binding sitei39ATPBy similarity1
Binding sitei93ATPBy similarity1
Binding sitei142ATPBy similarity1
Binding sitei149ATPBy similarity1
Metal bindingi223Divalent metal cationBy similarity1
Binding sitei238ATPBy similarity1
Binding sitei244ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi32 – 35ATPBy similarity4
Nucleotide bindingi55 – 57ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processDNA damage, DNA recombination, DNA repair, DNA replication
LigandATP-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase (EC:6.5.1.1PROSITE-ProRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene namesi
Name:1.3
OrganismiEnterobacteria phage T3 (Bacteriophage T3)
Taxonomic identifieri10759 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000595971 – 346DNA ligaseAdd BLAST346

Structurei

3D structure databases

ProteinModelPortaliP07717.
SMRiP07717.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated

Phylogenomic databases

KOiK18951.

Family and domain databases

InterProiView protein in InterPro
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR016306. DNA_ligase_ATP-dep_T3.
IPR012340. NA-bd_OB-fold.
PfamiView protein in Pfam
PF01068. DNA_ligase_A_M. 1 hit.
PIRSFiPIRSF001600. DNA_ligase_phage_T3. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiView protein in PROSITE
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.

Sequencei

Sequence statusi: Complete.

P07717-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIFNTNPFK AVSFVESAVK KALETSGYLI ADCKYDGVRG NIVVDNVAEA
60 70 80 90 100
AWLSRVSKFI PALEHLNGFD KRWQQLLNDD RCIFPDGFML DGELMVKGVD
110 120 130 140 150
FNTGSGLLRT KWVKRDNMGF HLTNVPTKLT PKGREVIDGK FEFHLDPKRL
160 170 180 190 200
SVRLYAVMPI HIAESGEDYD VQNLLMPYHV EAMRSLLVEY FPEIEWLIAE
210 220 230 240 250
TYEVYDMDSL TELYEEKRAE GHEGLIVKDP QGIYKRGKKS GWWKLKPECE
260 270 280 290 300
ADGIIQGVNW GTEGLANEGK VIGFSVLLET GRLVDANNIS RALMDEFTSN
310 320 330 340
VKAHGEDFYN GWACQVNYME ATPDGSLRHP SFEKFRGTED NPQEKM
Length:346
Mass (Da):39,351
Last modified:April 1, 1988 - v1
Checksum:i896D38E6AB80EE3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17255 Genomic DNA. Translation: CAA35125.1.
X05031 Genomic DNA. Translation: CAA28700.1.
PIRiS09539.
RefSeqiNP_523305.1. NC_003298.1.

Genome annotation databases

GeneIDi927441.
KEGGivg:927441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17255 Genomic DNA. Translation: CAA35125.1.
X05031 Genomic DNA. Translation: CAA28700.1.
PIRiS09539.
RefSeqiNP_523305.1. NC_003298.1.

3D structure databases

ProteinModelPortaliP07717.
SMRiP07717.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi927441.
KEGGivg:927441.

Phylogenomic databases

KOiK18951.

Family and domain databases

InterProiView protein in InterPro
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR016306. DNA_ligase_ATP-dep_T3.
IPR012340. NA-bd_OB-fold.
PfamiView protein in Pfam
PF01068. DNA_ligase_A_M. 1 hit.
PIRSFiPIRSF001600. DNA_ligase_phage_T3. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiView protein in PROSITE
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDNLI_BPT3
AccessioniPrimary (citable) accession number: P07717
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: February 15, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.