Reviewed, UniProtKB/Swiss-Prot P07717 (DNLI_BPT3)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:

1.3

Organism

Enterobacteria phage T3 (Bacteriophage T3)

Taxonomic identifier

10759 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Autographivirinae › T7-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase, which is expressed in the early stage of lytic development, has been implicated in T7 DNA synthesis and genetic recombination. It may also play a role in T7 DNA repair.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	DNA ligase		PRO_0000059597
Regions
	Nucleotide binding	32 – 35	4	ATP By similarity
	Nucleotide binding	55 – 57	3	ATP By similarity
Sites
	Active site	34	1	N6-AMP-lysine intermediate By similarity
	Metal binding	223	1	Divalent metal cation By similarity
	Binding site	39	1	ATP By similarity
	Binding site	93	1	ATP By similarity
	Binding site	142	1	ATP By similarity
	Binding site	149	1	ATP By similarity
	Binding site	238	1	ATP By similarity
	Binding site	244	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07717-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 896D38E6AB80EE3B	FASTA	346	39,351
	10 20 30 40 50 60 MNIFNTNPFK AVSFVESAVK KALETSGYLI ADCKYDGVRG NIVVDNVAEA AWLSRVSKFI 70 80 90 100 110 120 PALEHLNGFD KRWQQLLNDD RCIFPDGFML DGELMVKGVD FNTGSGLLRT KWVKRDNMGF 130 140 150 160 170 180 HLTNVPTKLT PKGREVIDGK FEFHLDPKRL SVRLYAVMPI HIAESGEDYD VQNLLMPYHV 190 200 210 220 230 240 EAMRSLLVEY FPEIEWLIAE TYEVYDMDSL TELYEEKRAE GHEGLIVKDP QGIYKRGKKS 250 260 270 280 290 300 GWWKLKPECE ADGIIQGVNW GTEGLANEGK VIGFSVLLET GRLVDANNIS RALMDEFTSN 310 320 330 340 VKAHGEDFYN GWACQVNYME ATPDGSLRHP SFEKFRGTED NPQEKM

References

[1]

"Sequence of a conditionally essential region of bacteriophage T3, including the primary origin of DNA replication." Schmitt M.P., Beck P.J., Kearney C.A., Spence J.L., Digiovanni D., Condreay J.P., Molineux I.J. J. Mol. Biol. 193:479-495(1987) [PubMed: 3586029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Luria.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17255 Genomic DNA. Translation: CAA35125.1. X05031 Genomic DNA. Translation: CAA28700.1.
PIR	S09539.
RefSeq	NP_523305.1.
3D structure databases
HSSP	HSSP built from PDB template 1A0I based on UniProtKB P00969.
SMR	P07717. Positions 6-336.
ModBase	Search...
Genome annotation databases
GeneID	927441.
Enzyme and pathway databases
BRENDA	6.5.1.1. 164907.
Family and domain databases
InterPro	IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR016306. DNA_ligase_phage. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
PIRSF	PIRSF001600. DNA_ligase_phage_T3. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_BPT3

Accession

Primary (citable) accession number: P07717

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)