Reviewed, UniProtKB/Swiss-Prot P07703 (RPAC1_YEAST)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerases I and III subunit RPAC1
      Short name=RNA polymerases I and III subunit AC1
Alternative name(s):
    DNA-directed RNA polymerases I and III 40 kDa polypeptide
      Short name=AC40
      Short name=C40
    C37

Gene names

Name:	RPC40
Synonyms:	RPA5, RPC5
Ordered Locus Names:	YPR110C
ORF Names:	P8283.18

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.
Subunit structure	Component of the RNA polymerase I (Pol I) and RNA polymerase III (Pol III) complexes consisting of at least 13 and 17 subunits, respectively. Interacts with the RPC19/RPAC2 and RPC53/RPC4. Ref.5
Subcellular location	Nucleus › nucleolus Ref.8.
Miscellaneous	Present with 13000 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the archaeal rpoD/eukaryotic RPB3 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	DNA-directed RNA polymerase I complex Traceable author statement. Source: SGD DNA-directed RNA polymerase III complex Traceable author statement. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Traceable author statement. Source: SGD protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NET1	P47035	1	EBI-15831,EBI-25953
RPA1	P10964	1	EBI-15831,EBI-15730
RPC19	P28000	3	EBI-15831,EBI-15846

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	DNA-directed RNA polymerases I and III subunit RPAC1		PRO_0000132742
Amino acid modifications
	Modified residue	15	1	Phosphothreonine Ref.10
	Modified residue	16	1	Phosphothreonine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P07703-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 76A5D6784403E990	FASTA	335	37,687
	10 20 30 40 50 60 MSNIVGIEYN RVTNTTSTDF PGFSKDAENE WNVEKFKKDF EVNISSLDAR EANFDLINID 70 80 90 100 110 120 TSIANAFRRI MISEVPSVAA EYVYFFNNTS VIQDEVLAHR IGLVPLKVDP DMLTWVDSNL 130 140 150 160 170 180 PDDEKFTDEN TIVLSLNVKC TRNPDAPKGS TDPKELYNNA HVYARDLKFE PQGRQSTTFA 190 200 210 220 230 240 DCPVVPADPD ILLAKLRPGQ EISLKAHCIL GIGGDHAKFS PVSTASYRLL PQINILQPIK 250 260 270 280 290 300 GESARRFQKC FPPGVIGIDE GSDEAYVKDA RKDTVSREVL RYEEFADKVK LGRVRNHFIF 310 320 330 NVESAGAMTP EEIFFKSVRI LKNKAEYLKN CPITQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RPC40, a unique gene for a subunit shared between yeast RNA polymerases A and C." Mann C., Buhler J.-M., Treich I., Sentenac A. Cell 48:627-637(1987) [PubMed: 3815519] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Interactions between three common subunits of yeast RNA polymerases I and III." Lalo D., Carles C., Sentenac A., Thuriaux P. Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed: 8516295] [Abstract] Cited for: ASSOCIATION WITH AC19.
[4]	"The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation." Chedin S., Ferri M.L., Peyroche G., Andrau J.-C., Jourdain S., Lefebvre O., Werner M., Carles C., Sentenac A. Cold Spring Harb. Symp. Quant. Biol. 63:381-389(1998) [PubMed: 10384303] [Abstract] Cited for: REVIEW ON THE RNA POL III COMPLEX, PHOSPHORYLATION.
[5]	"A protein-protein interaction map of yeast RNA polymerase III." Flores A., Briand J.-F., Gadal O., Andrau J.-C., Rubbi L., Van Mullem V., Boschiero C., Goussot M., Marck C., Carles C., Thuriaux P., Sentenac A., Werner M. Proc. Natl. Acad. Sci. U.S.A. 96:7815-7820(1999) [PubMed: 10393904] [Abstract] Cited for: INTERACTION WITH C53.
[6]	"Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I." Fath S., Milkereit P., Peyroche G., Riva M., Carles C., Tschochner H. Proc. Natl. Acad. Sci. U.S.A. 98:14334-14339(2001) [PubMed: 11717393] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
[7]	"The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits." Peyroche G., Levillain E., Siaut M., Callebaut I., Schultz P., Sentenac A., Riva M., Carles C. Proc. Natl. Acad. Sci. U.S.A. 99:14670-14675(2002) [PubMed: 12407181] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-15 AND THR-16, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M15499 Genomic DNA. Translation: AAA34999.1. U32445 Genomic DNA. Translation: AAB68080.1.
PIR	A25968.
RefSeq	NP_015435.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-17N.
IntAct	P07703. 90 interactions.
STRING	P07703.
Proteomic databases
PeptideAtlas	P07703.
PRIDE	P07703.
Genome annotation databases
Ensembl	YPR110C; YPR110C; YPR110C; Saccharomyces cerevisiae. [Genome view]
GeneID	856226.
KEGG	sce:YPR110C.
NMPDR	fig|4932.3.peg.6572.
Organism-specific databases
CYGD	YPR110c.
SGD	S000006314. RPC40.
Phylogenomic databases
HOGENOM	HBG539019.
OMA	VSRECLR.
OrthoDB	EOG9S4QZN.
Gene expression databases
ArrayExpress	P07703.
Genevestigator	P07703.
GermOnline	YPR110C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001514. DNA-dir_RNA_pol_30-40kDasu_CS. IPR011261. DNA-dir_RNA_pol_dimersation. IPR011262. DNA-dir_RNA_pol_insert. IPR009025. DNA-dir_RNA_pol_RBP11-like. IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3. [Graphical view]
Gene3D	G3DSA:2.170.120.12. RNAP_insert. 1 hit.
Pfam	PF01000. RNA_pol_A_bac. 1 hit. PF01193. RNA_pol_L. 1 hit. [Graphical view]
SMART	SM00662. RPOLD. 1 hit. [Graphical view]
PROSITE	PS00446. RNA_POL_D_30KD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981464.

Entry information

Entry name

RPAC1_YEAST

Accession

Primary (citable) accession number: P07703

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	December 15, 2009
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)