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P07703 (RPAC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerases I and III subunit RPAC1

Short name=RNA polymerases I and III subunit AC1
Alternative name(s):
C37
DNA-directed RNA polymerases I and III 40 kDa polypeptide
Short name=AC40
Short name=C40
Gene names
Name:RPC40
Synonyms:RPA5, RPC5
Ordered Locus Names:YPR110C
ORF Names:P8283.18
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I (Pol I) and III (Pol III) which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPC40 is part of the polymerase core and may function as a clamp element that moves to open and close the cleft. Ref.14 Ref.15 Ref.16

Subunit structure

Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Component of the RNA polymerase III (Pol III) complex consisting of at least 17 subunits. Interacts with the RPC19/RPAC2 and RPC53/RPC4. Ref.6 Ref.7 Ref.8 Ref.14 Ref.15 Ref.16

Subcellular location

Nucleusnucleolus Ref.9.

Miscellaneous

Present with 13000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RPA190P109645EBI-15831,EBI-15730
RPC19P280003EBI-15831,EBI-15846

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.13
Chain2 – 335334DNA-directed RNA polymerases I and III subunit RPAC1
PRO_0000132742

Amino acid modifications

Modified residue21N-acetylserine Ref.13
Modified residue171Phosphoserine Ref.12

Secondary structure

..................................................................... 335
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07703 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 76A5D6784403E990

FASTA33537,687
        10         20         30         40         50         60 
MSNIVGIEYN RVTNTTSTDF PGFSKDAENE WNVEKFKKDF EVNISSLDAR EANFDLINID 

        70         80         90        100        110        120 
TSIANAFRRI MISEVPSVAA EYVYFFNNTS VIQDEVLAHR IGLVPLKVDP DMLTWVDSNL 

       130        140        150        160        170        180 
PDDEKFTDEN TIVLSLNVKC TRNPDAPKGS TDPKELYNNA HVYARDLKFE PQGRQSTTFA 

       190        200        210        220        230        240 
DCPVVPADPD ILLAKLRPGQ EISLKAHCIL GIGGDHAKFS PVSTASYRLL PQINILQPIK 

       250        260        270        280        290        300 
GESARRFQKC FPPGVIGIDE GSDEAYVKDA RKDTVSREVL RYEEFADKVK LGRVRNHFIF 

       310        320        330 
NVESAGAMTP EEIFFKSVRI LKNKAEYLKN CPITQ 

« Hide

References

« Hide 'large scale' references
[1]"RPC40, a unique gene for a subunit shared between yeast RNA polymerases A and C."
Mann C., Buhler J.-M., Treich I., Sentenac A.
Cell 48:627-637(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Interactions between three common subunits of yeast RNA polymerases I and III."
Lalo D., Carles C., Sentenac A., Thuriaux P.
Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSOCIATION WITH AC19.
[5]"The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation."
Chedin S., Ferri M.L., Peyroche G., Andrau J.-C., Jourdain S., Lefebvre O., Werner M., Carles C., Sentenac A.
Cold Spring Harb. Symp. Quant. Biol. 63:381-389(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON THE RNA POL III COMPLEX, PHOSPHORYLATION.
[6]"A protein-protein interaction map of yeast RNA polymerase III."
Flores A., Briand J.-F., Gadal O., Andrau J.-C., Rubbi L., Van Mullem V., Boschiero C., Goussot M., Marck C., Carles C., Thuriaux P., Sentenac A., Werner M.
Proc. Natl. Acad. Sci. U.S.A. 96:7815-7820(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH C53.
[7]"Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I."
Fath S., Milkereit P., Peyroche G., Riva M., Carles C., Tschochner H.
Proc. Natl. Acad. Sci. U.S.A. 98:14334-14339(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
[8]"The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits."
Peyroche G., Levillain E., Siaut M., Callebaut I., Schultz P., Sentenac A., Riva M., Carles C.
Proc. Natl. Acad. Sci. U.S.A. 99:14670-14675(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[14]"Functional architecture of RNA polymerase I."
Kuhn C.D., Geiger S.R., Baumli S., Gartmann M., Gerber J., Jennebach S., Mielke T., Tschochner H., Beckmann R., Cramer P.
Cell 131:1260-1272(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.00 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
[15]"Crystal structure of the 14-subunit RNA polymerase I."
Fernandez-Tornero C., Moreno-Morcillo M., Rashid U.J., Taylor N.M., Ruiz F.M., Gruene T., Legrand P., Steuerwald U., Muller C.W.
Nature 502:644-649(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
[16]"RNA polymerase I structure and transcription regulation."
Engel C., Sainsbury S., Cheung A.C., Kostrewa D., Cramer P.
Nature 502:650-655(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M15499 Genomic DNA. Translation: AAA34999.1.
U32445 Genomic DNA. Translation: AAB68080.1.
BK006949 Genomic DNA. Translation: DAA11525.1.
PIRA25968.
RefSeqNP_015435.1. NM_001184207.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4C2MX-ray2.80C/R1-335[»]
4C3HX-ray3.27C1-335[»]
4C3IX-ray3.0C1-335[»]
4C3JX-ray3.35C1-335[»]
ProteinModelPortalP07703.
SMRP07703. Positions 31-335.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36277. 184 interactions.
DIPDIP-17N.
IntActP07703. 51 interactions.
MINTMINT-395916.
STRING4932.YPR110C.

Proteomic databases

MaxQBP07703.
PaxDbP07703.
PeptideAtlasP07703.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR110C; YPR110C; YPR110C.
GeneID856226.
KEGGsce:YPR110C.

Organism-specific databases

CYGDYPR110c.
SGDS000006314. RPC40.

Phylogenomic databases

eggNOGCOG0202.
GeneTreeENSGT00550000075060.
HOGENOMHOG000230845.
KOK03027.
OMAGQWDSDE.
OrthoDBEOG776T14.

Enzyme and pathway databases

BioCycYEAST:G3O-34250-MONOMER.

Gene expression databases

GenevestigatorP07703.

Family and domain databases

Gene3D2.170.120.12. 1 hit.
InterProIPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
IPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR009025. RBP11-like_dimer.
[Graphical view]
PfamPF01000. RNA_pol_A_bac. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
SMARTSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
PROSITEPS00446. RNA_POL_D_30KD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981464.
PROP07703.

Entry information

Entry nameRPAC1_YEAST
AccessionPrimary (citable) accession number: P07703
Secondary accession number(s): D6W4A9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 11, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references