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P07703

- RPAC1_YEAST

UniProt

P07703 - RPAC1_YEAST

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Protein

DNA-directed RNA polymerases I and III subunit RPAC1

Gene

RPC40

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I (Pol I) and III (Pol III) which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPC40 is part of the polymerase core and may function as a clamp element that moves to open and close the cleft.3 Publications

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. RNA polymerase I activity Source: UniProtKB

GO - Biological processi

  1. ribosome biogenesis Source: UniProtKB-KW
  2. transcription from RNA polymerase I promoter Source: UniProtKB
  3. tRNA transcription from RNA polymerase III promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-34250-MONOMER.
ReactomeiREACT_268966. RNA Polymerase III Transcription Termination.
REACT_269654. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
REACT_269997. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
REACT_270854. RNA Polymerase III Abortive And Retractive Initiation.
REACT_270979. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
REACT_271172. RNA Polymerase III Chain Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I and III subunit RPAC1
Short name:
RNA polymerases I and III subunit AC1
Alternative name(s):
C37
DNA-directed RNA polymerases I and III 40 kDa polypeptide
Short name:
AC40
Short name:
C40
Gene namesi
Name:RPC40
Synonyms:RPA5, RPC5
Ordered Locus Names:YPR110C
ORF Names:P8283.18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR110c.
SGDiS000006314. RPC40.

Subcellular locationi

Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. DNA-directed RNA polymerase I complex Source: UniProtKB
  2. DNA-directed RNA polymerase III complex Source: SGD
  3. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 335334DNA-directed RNA polymerases I and III subunit RPAC1PRO_0000132742Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei17 – 171Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP07703.
PaxDbiP07703.
PeptideAtlasiP07703.

Expressioni

Gene expression databases

GenevestigatoriP07703.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Component of the RNA polymerase III (Pol III) complex consisting of at least 17 subunits. Interacts with the RPC19/RPAC2 and RPC53/RPC4.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RPA190P109645EBI-15831,EBI-15730
RPC19P280003EBI-15831,EBI-15846

Protein-protein interaction databases

BioGridi36277. 184 interactions.
DIPiDIP-17N.
IntActiP07703. 51 interactions.
MINTiMINT-395916.
STRINGi4932.YPR110C.

Structurei

Secondary structure

1
335
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi33 – 397Combined sources
Beta strandi41 – 466Combined sources
Beta strandi49 – 5810Combined sources
Helixi61 – 7313Combined sources
Beta strandi77 – 8812Combined sources
Beta strandi90 – 923Combined sources
Helixi94 – 10310Combined sources
Helixi110 – 1123Combined sources
Beta strandi118 – 1203Combined sources
Helixi122 – 1254Combined sources
Turni128 – 1303Combined sources
Beta strandi132 – 1398Combined sources
Helixi153 – 1564Combined sources
Beta strandi157 – 1593Combined sources
Beta strandi161 – 1633Combined sources
Helixi164 – 1663Combined sources
Beta strandi168 – 1703Combined sources
Helixi175 – 1784Combined sources
Turni179 – 1813Combined sources
Beta strandi185 – 1884Combined sources
Beta strandi192 – 1954Combined sources
Beta strandi201 – 21111Combined sources
Turni213 – 2153Combined sources
Helixi217 – 2193Combined sources
Beta strandi222 – 23716Combined sources
Helixi242 – 2487Combined sources
Turni253 – 2553Combined sources
Beta strandi256 – 2583Combined sources
Turni260 – 2623Combined sources
Beta strandi264 – 2685Combined sources
Helixi270 – 2723Combined sources
Helixi278 – 2814Combined sources
Helixi283 – 2864Combined sources
Beta strandi289 – 30416Combined sources
Beta strandi306 – 3083Combined sources
Helixi310 – 32920Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4C2MX-ray2.80C/R1-335[»]
4C3HX-ray3.27C1-335[»]
4C3IX-ray3.0C1-335[»]
4C3JX-ray3.35C1-335[»]
ProteinModelPortaliP07703.
SMRiP07703. Positions 31-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0202.
GeneTreeiENSGT00550000075060.
HOGENOMiHOG000230845.
InParanoidiP07703.
KOiK03027.
OMAiGQWDSDE.
OrthoDBiEOG776T14.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
InterProiIPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
IPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR009025. RBP11-like_dimer.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
PROSITEiPS00446. RNA_POL_D_30KD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07703-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNIVGIEYN RVTNTTSTDF PGFSKDAENE WNVEKFKKDF EVNISSLDAR
60 70 80 90 100
EANFDLINID TSIANAFRRI MISEVPSVAA EYVYFFNNTS VIQDEVLAHR
110 120 130 140 150
IGLVPLKVDP DMLTWVDSNL PDDEKFTDEN TIVLSLNVKC TRNPDAPKGS
160 170 180 190 200
TDPKELYNNA HVYARDLKFE PQGRQSTTFA DCPVVPADPD ILLAKLRPGQ
210 220 230 240 250
EISLKAHCIL GIGGDHAKFS PVSTASYRLL PQINILQPIK GESARRFQKC
260 270 280 290 300
FPPGVIGIDE GSDEAYVKDA RKDTVSREVL RYEEFADKVK LGRVRNHFIF
310 320 330
NVESAGAMTP EEIFFKSVRI LKNKAEYLKN CPITQ
Length:335
Mass (Da):37,687
Last modified:April 1, 1988 - v1
Checksum:i76A5D6784403E990
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15499 Genomic DNA. Translation: AAA34999.1.
U32445 Genomic DNA. Translation: AAB68080.1.
BK006949 Genomic DNA. Translation: DAA11525.1.
PIRiA25968.
RefSeqiNP_015435.1. NM_001184207.1.

Genome annotation databases

EnsemblFungiiYPR110C; YPR110C; YPR110C.
GeneIDi856226.
KEGGisce:YPR110C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15499 Genomic DNA. Translation: AAA34999.1 .
U32445 Genomic DNA. Translation: AAB68080.1 .
BK006949 Genomic DNA. Translation: DAA11525.1 .
PIRi A25968.
RefSeqi NP_015435.1. NM_001184207.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4C2M X-ray 2.80 C/R 1-335 [» ]
4C3H X-ray 3.27 C 1-335 [» ]
4C3I X-ray 3.0 C 1-335 [» ]
4C3J X-ray 3.35 C 1-335 [» ]
ProteinModelPortali P07703.
SMRi P07703. Positions 31-335.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36277. 184 interactions.
DIPi DIP-17N.
IntActi P07703. 51 interactions.
MINTi MINT-395916.
STRINGi 4932.YPR110C.

Proteomic databases

MaxQBi P07703.
PaxDbi P07703.
PeptideAtlasi P07703.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR110C ; YPR110C ; YPR110C .
GeneIDi 856226.
KEGGi sce:YPR110C.

Organism-specific databases

CYGDi YPR110c.
SGDi S000006314. RPC40.

Phylogenomic databases

eggNOGi COG0202.
GeneTreei ENSGT00550000075060.
HOGENOMi HOG000230845.
InParanoidi P07703.
KOi K03027.
OMAi GQWDSDE.
OrthoDBi EOG776T14.

Enzyme and pathway databases

BioCyci YEAST:G3O-34250-MONOMER.
Reactomei REACT_268966. RNA Polymerase III Transcription Termination.
REACT_269654. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
REACT_269997. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
REACT_270854. RNA Polymerase III Abortive And Retractive Initiation.
REACT_270979. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
REACT_271172. RNA Polymerase III Chain Elongation.

Miscellaneous databases

NextBioi 981464.
PROi P07703.

Gene expression databases

Genevestigatori P07703.

Family and domain databases

Gene3Di 2.170.120.12. 1 hit.
InterProi IPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
IPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR009025. RBP11-like_dimer.
[Graphical view ]
Pfami PF01000. RNA_pol_A_bac. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view ]
SMARTi SM00662. RPOLD. 1 hit.
[Graphical view ]
SUPFAMi SSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
PROSITEi PS00446. RNA_POL_D_30KD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "RPC40, a unique gene for a subunit shared between yeast RNA polymerases A and C."
    Mann C., Buhler J.-M., Treich I., Sentenac A.
    Cell 48:627-637(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Interactions between three common subunits of yeast RNA polymerases I and III."
    Lalo D., Carles C., Sentenac A., Thuriaux P.
    Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: ASSOCIATION WITH AC19.
  5. "The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation."
    Chedin S., Ferri M.L., Peyroche G., Andrau J.-C., Jourdain S., Lefebvre O., Werner M., Carles C., Sentenac A.
    Cold Spring Harb. Symp. Quant. Biol. 63:381-389(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON THE RNA POL III COMPLEX, PHOSPHORYLATION.
  6. Cited for: INTERACTION WITH C53.
  7. "Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I."
    Fath S., Milkereit P., Peyroche G., Riva M., Carles C., Tschochner H.
    Proc. Natl. Acad. Sci. U.S.A. 98:14334-14339(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
  8. "The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits."
    Peyroche G., Levillain E., Siaut M., Callebaut I., Schultz P., Sentenac A., Riva M., Carles C.
    Proc. Natl. Acad. Sci. U.S.A. 99:14670-14675(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  14. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.00 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
  15. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
  16. "RNA polymerase I structure and transcription regulation."
    Engel C., Sainsbury S., Cheung A.C., Kostrewa D., Cramer P.
    Nature 502:650-655(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.

Entry informationi

Entry nameiRPAC1_YEAST
AccessioniPrimary (citable) accession number: P07703
Secondary accession number(s): D6W4A9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 26, 2014
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3