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P07702 (LYS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
L-aminoadipate-semialdehyde dehydrogenase

EC=1.2.1.31
Alternative name(s):
Alpha-aminoadipate reductase
Short name=Alpha-AR
Gene names
Name:LYS2
Ordered Locus Names:YBR115C
ORF Names:YBR0910
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1392 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH.

Catalytic activity

(S)-2-amino-6-oxohexanoate + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H.

Cofactor

Binds 1 phosphopantetheine covalently.

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 1/3.

Miscellaneous

Present with 7430 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Contains 1 acyl carrier domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13921392L-aminoadipate-semialdehyde dehydrogenase
PRO_0000193153

Regions

Domain848 – 91770Acyl carrier

Amino acid modifications

Modified residue8801O-(pantetheine 4'-phosphoryl)serine

Sequences

Sequence LengthMass (Da)Tools
P07702 [UniParc].

Last modified March 1, 1992. Version 2.
Checksum: F0083A80BC6F7FB5

FASTA1,392155,346
        10         20         30         40         50         60 
MTNEKVWIEK LDNPTLSVLP HDFLRPQQEP YTKQATYSLQ LPQLDVPHDS FSNKYAVALS 

        70         80         90        100        110        120 
VWAALIYRVT GDDDIVLYIA NNKILRFNIQ PTWSFNELYS TINNELNKLN SIEANFSFDE 

       130        140        150        160        170        180 
LAEKIQSCQD LERTPQLFRL AFLENQDFKL DEFKHHLVDF ALNLDTSNNA HVLNLIYNSL 

       190        200        210        220        230        240 
LYSNERVTIV ADQFTQYLTA ALSDPSNCIT KISLITASSK DSLPDPTKNL GWCDFVGCIH 

       250        260        270        280        290        300 
DIFQDNAEAF PERTCVVETP TLNSDKSRSF TYRDINRTSN IVAHYLIKTG IKRGDVVMIY 

       310        320        330        340        350        360 
SSRGVDLMVC VMGVLKAGAT FSVIDPAYPP ARQTIYLGVA KPRGLIVIRA AGQLDQLVED 

       370        380        390        400        410        420 
YINDELEIVS RINSIAIQEN GTIEGGKLDN GEDVLAPYDH YKDTRTGVVV GPDSNPTLSF 

       430        440        450        460        470        480 
TSGSEGIPKG VLGRHFSLAY YFNWMSKRFN LTENDKFTML SGIAHDPIQR DMFTPLFLGA 

       490        500        510        520        530        540 
QLYVPTQDDI GTPGRLAEWM SKYGCTVTHL TPAMGQLLTA QATTPFPKLH HAFFVGDILT 

       550        560        570        580        590        600 
KRDCLRLQTL AENCRIVNMY GTTETQRAVS YFEVKSKNDD PNFLKKLKDV MPAGKGMLNV 

       610        620        630        640        650        660 
QLLVVNRNDR TQICGIGEIG EIYVRAGGLA EGYRGLPELN KEKFVNNWFV EKDHWNYLDK 

       670        680        690        700        710        720 
DNGEPWRQFW LGPRDRLYRT GDLGRYLPNG DCECCGRADD QVKIRGFRIE LGEIDTHISQ 

       730        740        750        760        770        780 
HPLVRENITL VRKNADNEPT LITFMVPRFD KPDDLSKFQS DVPKEVETDP IVKGLIGYHL 

       790        800        810        820        830        840 
LSKDIRTFLK KRLASYAMPS LIVVMDKLPL NPNGKVDKPK LQFPTPKQLN LVAENTVSET 

       850        860        870        880        890        900 
DDSQFTNVER EVRDLWLSIL PTKPASVSPD DSFFDLGGHS ILATKMIFTL KKKLQVDLPL 

       910        920        930        940        950        960 
GTIFKYPTIK AFAAEIDRIK SSGGSSQGEV VENVTANYAE DAKKLVETLP SSYPSREYFV 

       970        980        990       1000       1010       1020 
EPNSAEGKTT INVFVTGVTG FLGSYILADL LGRSPKNYSF KVFAHVRAKD EEAAFARLQK 

      1030       1040       1050       1060       1070       1080 
AGITYGTWNE KFASNIKVVL GDLSKSQFGL SDEKWMDLAN TVDIIIHNGA LVHWVYPYAK 

      1090       1100       1110       1120       1130       1140 
LRDPNVISTI NVMSLAAVGK PKFFDFVSST STLDTEYYFN LSDKLVSEGK PGILESDDLM 

      1150       1160       1170       1180       1190       1200 
NSASGLTGGY GQSKWAAEYI IRRAGERGLR GCIVRPGYVT GASANGSSNT DDFLLRFLKG 

      1210       1220       1230       1240       1250       1260 
SVQLGKIPDI ENSVNMVPVD HVARVVVATS LNPPKENELA VAQVTGHPRI LFKDYLYTLH 

      1270       1280       1290       1300       1310       1320 
DYGYDVEIES YSKWKKSLEA SVIDRNEENA LYPLLHMVLD NLPESTKAPE LDDRNAVASL 

      1330       1340       1350       1360       1370       1380 
KKDTAWTGVD WSNGIGVTPE EVGIYIAFLN KVGFLPPPTH NDKLPLPSIE LTQAQISLVA 

      1390 
SGAGARGSSA AA 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the LYS2 gene of Saccharomyces cerevisiae: homology to Bacillus brevis tyrocidine synthetase 1."
Morris M.E., Jinks-Robertson S.
Gene 98:141-145(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of a 70 kb region on the right arm of yeast chromosome II."
Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Construction of LYS2 cartridges for use in genetic manipulations of Saccharomyces cerevisiae."
Fleig U.N., Pridmore R.D., Philippsen P.
Gene 46:237-245(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-150 AND 1209-1392.
[6]"Molecular analysis of yeast chromosome II between CMD1 and LYS2: the excision repair gene RAD16 located in this region belongs to a novel group of double-finger proteins."
Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
Yeast 8:397-408(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1083-1392.
Strain: ATCC 204508 / S288c.
[7]"TKL2, a second transketolase gene of Saccharomyces cerevisiae. Cloning, sequence and deletion analysis of the gene."
Schaaff-Gerstenschlaeger I., Mannhaupt G., Vetter I., Zimmermann F.K., Feldmann H.
Eur. J. Biochem. 217:487-492(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-130.
Strain: ATCC 204508 / S288c.
[8]"Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5."
Ehmann D.E., Gehring A.M., Walsh C.T.
Biochemistry 38:6171-6177(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHOPANTETHEINYLATION AT SER-880, IDENTIFICATION BY MASS SPECTROMETRY.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M36287 Genomic DNA. Translation: AAA34747.1.
X66247 Genomic DNA. Translation: CAA46975.1.
X78993 Genomic DNA. Translation: CAA55617.1.
Z35984 Genomic DNA. Translation: CAA85072.1.
X73532 Genomic DNA. Translation: CAA51938.1.
BK006936 Genomic DNA. Translation: DAA07234.1.
PIRYGBYAD. JU0448.
RefSeqNP_009673.1. NM_001178463.1.

3D structure databases

ProteinModelPortalP07702.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32818. 22 interactions.
DIPDIP-6811N.
IntActP07702. 4 interactions.
MINTMINT-661149.
STRING4932.YBR115C.

Proteomic databases

PaxDbP07702.
PeptideAtlasP07702.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR115C; YBR115C; YBR115C.
GeneID852412.
KEGGsce:YBR115C.

Organism-specific databases

CYGDYBR115c.
SGDS000000319. LYS2.

Phylogenomic databases

eggNOGCOG3320.
GeneTreeENSGT00650000093222.
HOGENOMHOG000191209.
KOK00143.
OMADRFTMLS.
OrthoDBEOG74TX6Z.

Enzyme and pathway databases

BioCycYEAST:YBR115C-MONOMER.
UniPathwayUPA00033; UER00032.

Gene expression databases

GenevestigatorP07702.

Family and domain databases

Gene3D1.10.1200.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR010071. AA_adenyl_domain.
IPR009081. Acyl_carrier_prot-like.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR014397. L-NH2adipate-semiAld_DH_lsu.
IPR013120. Male_sterile_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR006162. PPantetheine_attach_site.
IPR010080. Thioester_reductase-like_dom.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
PF07993. NAD_binding_4. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
PIRSFPIRSF001617. Alpha-AR. 1 hit.
SUPFAMSSF47336. SSF47336. 1 hit.
TIGRFAMsTIGR01733. AA-adenyl-dom. 1 hit.
TIGR03443. alpha_am_amid. 1 hit.
TIGR01746. Thioester-redct. 1 hit.
PROSITEPS50075. ACP_DOMAIN. 1 hit.
PS00455. AMP_BINDING. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971264.

Entry information

Entry nameLYS2_YEAST
AccessionPrimary (citable) accession number: P07702
Secondary accession number(s): D6VQB4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: March 1, 1992
Last modified: April 16, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways