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Protein

L-2-aminoadipate reductase

Gene

LYS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.1 Publication

Miscellaneous

Present with 7430 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(S)-2-amino-6-oxohexanoate + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H.1 Publication
(S)-2-amino-6-oxohexanoate + NADP+ + AMP + diphosphate = L-2-aminoadipate + NADPH + ATP.1 Publication

Cofactori

pantetheine 4'-phosphate1 PublicationNote: Binds 1 phosphopantetheine covalently.1 Publication

Kineticsi

kcat is 670 min(-1).1 Publication
  1. KM=620 µM for NADPH1 Publication

    Pathwayi: L-lysine biosynthesis via AAA pathway

    This protein is involved in step 1 of the subpathway that synthesizes L-lysine from L-alpha-aminoadipate (fungal route).
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. L-2-aminoadipate reductase (LYS2)
    2. Saccharopine dehydrogenase [NADP(+), L-glutamate-forming] (LYS9)
    3. Saccharopine dehydrogenase [NAD(+), L-lysine-forming] (LYS1)
    This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-lysine from L-alpha-aminoadipate (fungal route), the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

    GO - Molecular functioni

    GO - Biological processi

    • lysine biosynthetic process via aminoadipic acid Source: SGD

    Keywordsi

    Molecular functionOxidoreductase
    Biological processAmino-acid biosynthesis, Lysine biosynthesis
    LigandNADP

    Enzyme and pathway databases

    BioCyciMetaCyc:YBR115C-MONOMER
    YEAST:YBR115C-MONOMER
    ReactomeiR-SCE-2142700 Synthesis of Lipoxins (LX)
    R-SCE-70895 Branched-chain amino acid catabolism
    R-SCE-75105 Fatty acyl-CoA biosynthesis
    R-SCE-9018676 Biosynthesis of D-series resolvins
    R-SCE-9018896 Biosynthesis of E-series 18(S)-resolvins
    SABIO-RKiP07702
    UniPathwayiUPA00033; UER00032

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-2-aminoadipate reductase (EC:1.2.1.311 Publication, EC:1.2.1.951 Publication)
    Alternative name(s):
    Alpha-aminoadipate reductase1 Publication
    Short name:
    Alpha-AR
    L-aminoadipate-semialdehyde dehydrogenase
    Gene namesi
    Name:LYS2
    Ordered Locus Names:YBR115C
    ORF Names:YBR0910
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome II

    Organism-specific databases

    EuPathDBiFungiDB:YBR115C
    SGDiS000000319 LYS2

    Subcellular locationi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001931531 – 1392L-2-aminoadipate reductaseAdd BLAST1392

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Cross-linki541Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
    Modified residuei880O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1 Publication1
    Cross-linki1276Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

    Keywords - PTMi

    Isopeptide bond, Phosphopantetheine, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP07702
    PaxDbiP07702
    PRIDEiP07702

    PTM databases

    CarbonylDBiP07702
    iPTMnetiP07702

    Interactioni

    Protein-protein interaction databases

    BioGridi32818, 25 interactors
    DIPiDIP-6811N
    IntActiP07702, 4 interactors
    MINTiP07702
    STRINGi4932.YBR115C

    Structurei

    3D structure databases

    ProteinModelPortaliP07702
    SMRiP07702
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini843 – 920CarrierPROSITE-ProRule annotationAdd BLAST78

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00440000033811
    HOGENOMiHOG000191209
    InParanoidiP07702
    KOiK00143
    OMAiMIYAYRG
    OrthoDBiEOG092C07V5

    Family and domain databases

    CDDicd05235 SDR_e1, 1 hit
    Gene3Di1.10.1200.10, 1 hit
    InterProiView protein in InterPro
    IPR010071 AA_adenyl_domain
    IPR036736 ACP-like_sf
    IPR020845 AMP-binding_CS
    IPR000873 AMP-dep_Synth/Lig
    IPR014397 Lys2
    IPR013120 Male_sterile_NAD-bd
    IPR036291 NAD(P)-bd_dom_sf
    IPR020806 PKS_PP-bd
    IPR009081 PP-bd_ACP
    IPR006162 Ppantetheine_attach_site
    IPR010080 Thioester_reductase-like_dom
    PfamiView protein in Pfam
    PF00501 AMP-binding, 1 hit
    PF07993 NAD_binding_4, 1 hit
    PF00550 PP-binding, 1 hit
    SMARTiView protein in SMART
    SM00823 PKS_PP, 1 hit
    SUPFAMiSSF47336 SSF47336, 1 hit
    SSF51735 SSF51735, 1 hit
    TIGRFAMsiTIGR01733 AA-adenyl-dom, 1 hit
    TIGR03443 alpha_am_amid, 1 hit
    TIGR01746 Thioester-redct, 1 hit
    PROSITEiView protein in PROSITE
    PS00455 AMP_BINDING, 1 hit
    PS50075 CARRIER, 1 hit
    PS00012 PHOSPHOPANTETHEINE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P07702-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTNEKVWIEK LDNPTLSVLP HDFLRPQQEP YTKQATYSLQ LPQLDVPHDS
    60 70 80 90 100
    FSNKYAVALS VWAALIYRVT GDDDIVLYIA NNKILRFNIQ PTWSFNELYS
    110 120 130 140 150
    TINNELNKLN SIEANFSFDE LAEKIQSCQD LERTPQLFRL AFLENQDFKL
    160 170 180 190 200
    DEFKHHLVDF ALNLDTSNNA HVLNLIYNSL LYSNERVTIV ADQFTQYLTA
    210 220 230 240 250
    ALSDPSNCIT KISLITASSK DSLPDPTKNL GWCDFVGCIH DIFQDNAEAF
    260 270 280 290 300
    PERTCVVETP TLNSDKSRSF TYRDINRTSN IVAHYLIKTG IKRGDVVMIY
    310 320 330 340 350
    SSRGVDLMVC VMGVLKAGAT FSVIDPAYPP ARQTIYLGVA KPRGLIVIRA
    360 370 380 390 400
    AGQLDQLVED YINDELEIVS RINSIAIQEN GTIEGGKLDN GEDVLAPYDH
    410 420 430 440 450
    YKDTRTGVVV GPDSNPTLSF TSGSEGIPKG VLGRHFSLAY YFNWMSKRFN
    460 470 480 490 500
    LTENDKFTML SGIAHDPIQR DMFTPLFLGA QLYVPTQDDI GTPGRLAEWM
    510 520 530 540 550
    SKYGCTVTHL TPAMGQLLTA QATTPFPKLH HAFFVGDILT KRDCLRLQTL
    560 570 580 590 600
    AENCRIVNMY GTTETQRAVS YFEVKSKNDD PNFLKKLKDV MPAGKGMLNV
    610 620 630 640 650
    QLLVVNRNDR TQICGIGEIG EIYVRAGGLA EGYRGLPELN KEKFVNNWFV
    660 670 680 690 700
    EKDHWNYLDK DNGEPWRQFW LGPRDRLYRT GDLGRYLPNG DCECCGRADD
    710 720 730 740 750
    QVKIRGFRIE LGEIDTHISQ HPLVRENITL VRKNADNEPT LITFMVPRFD
    760 770 780 790 800
    KPDDLSKFQS DVPKEVETDP IVKGLIGYHL LSKDIRTFLK KRLASYAMPS
    810 820 830 840 850
    LIVVMDKLPL NPNGKVDKPK LQFPTPKQLN LVAENTVSET DDSQFTNVER
    860 870 880 890 900
    EVRDLWLSIL PTKPASVSPD DSFFDLGGHS ILATKMIFTL KKKLQVDLPL
    910 920 930 940 950
    GTIFKYPTIK AFAAEIDRIK SSGGSSQGEV VENVTANYAE DAKKLVETLP
    960 970 980 990 1000
    SSYPSREYFV EPNSAEGKTT INVFVTGVTG FLGSYILADL LGRSPKNYSF
    1010 1020 1030 1040 1050
    KVFAHVRAKD EEAAFARLQK AGITYGTWNE KFASNIKVVL GDLSKSQFGL
    1060 1070 1080 1090 1100
    SDEKWMDLAN TVDIIIHNGA LVHWVYPYAK LRDPNVISTI NVMSLAAVGK
    1110 1120 1130 1140 1150
    PKFFDFVSST STLDTEYYFN LSDKLVSEGK PGILESDDLM NSASGLTGGY
    1160 1170 1180 1190 1200
    GQSKWAAEYI IRRAGERGLR GCIVRPGYVT GASANGSSNT DDFLLRFLKG
    1210 1220 1230 1240 1250
    SVQLGKIPDI ENSVNMVPVD HVARVVVATS LNPPKENELA VAQVTGHPRI
    1260 1270 1280 1290 1300
    LFKDYLYTLH DYGYDVEIES YSKWKKSLEA SVIDRNEENA LYPLLHMVLD
    1310 1320 1330 1340 1350
    NLPESTKAPE LDDRNAVASL KKDTAWTGVD WSNGIGVTPE EVGIYIAFLN
    1360 1370 1380 1390
    KVGFLPPPTH NDKLPLPSIE LTQAQISLVA SGAGARGSSA AA
    Length:1,392
    Mass (Da):155,346
    Last modified:March 1, 1992 - v2
    Checksum:iF0083A80BC6F7FB5
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M36287 Genomic DNA Translation: AAA34747.1
    X66247 Genomic DNA Translation: CAA46975.1
    X78993 Genomic DNA Translation: CAA55617.1
    Z35984 Genomic DNA Translation: CAA85072.1
    X73532 Genomic DNA Translation: CAA51938.1
    BK006936 Genomic DNA Translation: DAA07234.1
    PIRiJU0448 YGBYAD
    RefSeqiNP_009673.1, NM_001178463.1

    Genome annotation databases

    EnsemblFungiiYBR115C; YBR115C; YBR115C
    GeneIDi852412
    KEGGisce:YBR115C

    Similar proteinsi

    Entry informationi

    Entry nameiLYS2_YEAST
    AccessioniPrimary (citable) accession number: P07702
    Secondary accession number(s): D6VQB4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: March 1, 1992
    Last modified: June 20, 2018
    This is version 182 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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