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P07702

- LYS2_YEAST

UniProt

P07702 - LYS2_YEAST

Protein

L-aminoadipate-semialdehyde dehydrogenase

Gene

LYS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 148 (01 Oct 2014)
      Sequence version 2 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH.

    Catalytic activityi

    (S)-2-amino-6-oxohexanoate + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H.

    Cofactori

    Binds 1 phosphopantetheine covalently.

    Pathwayi

    GO - Molecular functioni

    1. L-aminoadipate-semialdehyde dehydrogenase activity Source: SGD
    2. phosphopantetheine binding Source: InterPro

    GO - Biological processi

    1. lysine biosynthetic process via aminoadipic acid Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciYEAST:YBR115C-MONOMER.
    UniPathwayiUPA00033; UER00032.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-aminoadipate-semialdehyde dehydrogenase (EC:1.2.1.31)
    Alternative name(s):
    Alpha-aminoadipate reductase
    Short name:
    Alpha-AR
    Gene namesi
    Name:LYS2
    Ordered Locus Names:YBR115C
    ORF Names:YBR0910
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR115c.
    SGDiS000000319. LYS2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13921392L-aminoadipate-semialdehyde dehydrogenasePRO_0000193153Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei880 – 8801O-(pantetheine 4'-phosphoryl)serine1 PublicationPROSITE-ProRule annotation

    Keywords - PTMi

    Phosphopantetheine, Phosphoprotein

    Proteomic databases

    MaxQBiP07702.
    PaxDbiP07702.
    PeptideAtlasiP07702.

    Expressioni

    Gene expression databases

    GenevestigatoriP07702.

    Interactioni

    Protein-protein interaction databases

    BioGridi32818. 22 interactions.
    DIPiDIP-6811N.
    IntActiP07702. 4 interactions.
    MINTiMINT-661149.
    STRINGi4932.YBR115C.

    Structurei

    3D structure databases

    ProteinModelPortaliP07702.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini848 – 91770Acyl carrierPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 acyl carrier domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG3320.
    GeneTreeiENSGT00650000093222.
    HOGENOMiHOG000191209.
    KOiK00143.
    OMAiDRFTMLS.
    OrthoDBiEOG74TX6Z.

    Family and domain databases

    Gene3Di1.10.1200.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR010071. AA_adenyl_domain.
    IPR009081. Acyl_carrier_prot-like.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    IPR014397. L-NH2adipate-semiAld_DH_lsu.
    IPR013120. Male_sterile_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR006162. PPantetheine_attach_site.
    IPR010080. Thioester_reductase-like_dom.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    PF07993. NAD_binding_4. 1 hit.
    PF00550. PP-binding. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001617. Alpha-AR. 1 hit.
    SUPFAMiSSF47336. SSF47336. 1 hit.
    TIGRFAMsiTIGR01733. AA-adenyl-dom. 1 hit.
    TIGR03443. alpha_am_amid. 1 hit.
    TIGR01746. Thioester-redct. 1 hit.
    PROSITEiPS50075. ACP_DOMAIN. 1 hit.
    PS00455. AMP_BINDING. 1 hit.
    PS00012. PHOSPHOPANTETHEINE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07702-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNEKVWIEK LDNPTLSVLP HDFLRPQQEP YTKQATYSLQ LPQLDVPHDS     50
    FSNKYAVALS VWAALIYRVT GDDDIVLYIA NNKILRFNIQ PTWSFNELYS 100
    TINNELNKLN SIEANFSFDE LAEKIQSCQD LERTPQLFRL AFLENQDFKL 150
    DEFKHHLVDF ALNLDTSNNA HVLNLIYNSL LYSNERVTIV ADQFTQYLTA 200
    ALSDPSNCIT KISLITASSK DSLPDPTKNL GWCDFVGCIH DIFQDNAEAF 250
    PERTCVVETP TLNSDKSRSF TYRDINRTSN IVAHYLIKTG IKRGDVVMIY 300
    SSRGVDLMVC VMGVLKAGAT FSVIDPAYPP ARQTIYLGVA KPRGLIVIRA 350
    AGQLDQLVED YINDELEIVS RINSIAIQEN GTIEGGKLDN GEDVLAPYDH 400
    YKDTRTGVVV GPDSNPTLSF TSGSEGIPKG VLGRHFSLAY YFNWMSKRFN 450
    LTENDKFTML SGIAHDPIQR DMFTPLFLGA QLYVPTQDDI GTPGRLAEWM 500
    SKYGCTVTHL TPAMGQLLTA QATTPFPKLH HAFFVGDILT KRDCLRLQTL 550
    AENCRIVNMY GTTETQRAVS YFEVKSKNDD PNFLKKLKDV MPAGKGMLNV 600
    QLLVVNRNDR TQICGIGEIG EIYVRAGGLA EGYRGLPELN KEKFVNNWFV 650
    EKDHWNYLDK DNGEPWRQFW LGPRDRLYRT GDLGRYLPNG DCECCGRADD 700
    QVKIRGFRIE LGEIDTHISQ HPLVRENITL VRKNADNEPT LITFMVPRFD 750
    KPDDLSKFQS DVPKEVETDP IVKGLIGYHL LSKDIRTFLK KRLASYAMPS 800
    LIVVMDKLPL NPNGKVDKPK LQFPTPKQLN LVAENTVSET DDSQFTNVER 850
    EVRDLWLSIL PTKPASVSPD DSFFDLGGHS ILATKMIFTL KKKLQVDLPL 900
    GTIFKYPTIK AFAAEIDRIK SSGGSSQGEV VENVTANYAE DAKKLVETLP 950
    SSYPSREYFV EPNSAEGKTT INVFVTGVTG FLGSYILADL LGRSPKNYSF 1000
    KVFAHVRAKD EEAAFARLQK AGITYGTWNE KFASNIKVVL GDLSKSQFGL 1050
    SDEKWMDLAN TVDIIIHNGA LVHWVYPYAK LRDPNVISTI NVMSLAAVGK 1100
    PKFFDFVSST STLDTEYYFN LSDKLVSEGK PGILESDDLM NSASGLTGGY 1150
    GQSKWAAEYI IRRAGERGLR GCIVRPGYVT GASANGSSNT DDFLLRFLKG 1200
    SVQLGKIPDI ENSVNMVPVD HVARVVVATS LNPPKENELA VAQVTGHPRI 1250
    LFKDYLYTLH DYGYDVEIES YSKWKKSLEA SVIDRNEENA LYPLLHMVLD 1300
    NLPESTKAPE LDDRNAVASL KKDTAWTGVD WSNGIGVTPE EVGIYIAFLN 1350
    KVGFLPPPTH NDKLPLPSIE LTQAQISLVA SGAGARGSSA AA 1392
    Length:1,392
    Mass (Da):155,346
    Last modified:March 1, 1992 - v2
    Checksum:iF0083A80BC6F7FB5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36287 Genomic DNA. Translation: AAA34747.1.
    X66247 Genomic DNA. Translation: CAA46975.1.
    X78993 Genomic DNA. Translation: CAA55617.1.
    Z35984 Genomic DNA. Translation: CAA85072.1.
    X73532 Genomic DNA. Translation: CAA51938.1.
    BK006936 Genomic DNA. Translation: DAA07234.1.
    PIRiJU0448. YGBYAD.
    RefSeqiNP_009673.1. NM_001178463.1.

    Genome annotation databases

    EnsemblFungiiYBR115C; YBR115C; YBR115C.
    GeneIDi852412.
    KEGGisce:YBR115C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36287 Genomic DNA. Translation: AAA34747.1 .
    X66247 Genomic DNA. Translation: CAA46975.1 .
    X78993 Genomic DNA. Translation: CAA55617.1 .
    Z35984 Genomic DNA. Translation: CAA85072.1 .
    X73532 Genomic DNA. Translation: CAA51938.1 .
    BK006936 Genomic DNA. Translation: DAA07234.1 .
    PIRi JU0448. YGBYAD.
    RefSeqi NP_009673.1. NM_001178463.1.

    3D structure databases

    ProteinModelPortali P07702.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32818. 22 interactions.
    DIPi DIP-6811N.
    IntActi P07702. 4 interactions.
    MINTi MINT-661149.
    STRINGi 4932.YBR115C.

    Proteomic databases

    MaxQBi P07702.
    PaxDbi P07702.
    PeptideAtlasi P07702.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR115C ; YBR115C ; YBR115C .
    GeneIDi 852412.
    KEGGi sce:YBR115C.

    Organism-specific databases

    CYGDi YBR115c.
    SGDi S000000319. LYS2.

    Phylogenomic databases

    eggNOGi COG3320.
    GeneTreei ENSGT00650000093222.
    HOGENOMi HOG000191209.
    KOi K00143.
    OMAi DRFTMLS.
    OrthoDBi EOG74TX6Z.

    Enzyme and pathway databases

    UniPathwayi UPA00033 ; UER00032 .
    BioCyci YEAST:YBR115C-MONOMER.

    Miscellaneous databases

    NextBioi 971264.

    Gene expression databases

    Genevestigatori P07702.

    Family and domain databases

    Gene3Di 1.10.1200.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR010071. AA_adenyl_domain.
    IPR009081. Acyl_carrier_prot-like.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    IPR014397. L-NH2adipate-semiAld_DH_lsu.
    IPR013120. Male_sterile_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR006162. PPantetheine_attach_site.
    IPR010080. Thioester_reductase-like_dom.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    PF07993. NAD_binding_4. 1 hit.
    PF00550. PP-binding. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001617. Alpha-AR. 1 hit.
    SUPFAMi SSF47336. SSF47336. 1 hit.
    TIGRFAMsi TIGR01733. AA-adenyl-dom. 1 hit.
    TIGR03443. alpha_am_amid. 1 hit.
    TIGR01746. Thioester-redct. 1 hit.
    PROSITEi PS50075. ACP_DOMAIN. 1 hit.
    PS00455. AMP_BINDING. 1 hit.
    PS00012. PHOSPHOPANTETHEINE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the LYS2 gene of Saccharomyces cerevisiae: homology to Bacillus brevis tyrocidine synthetase 1."
      Morris M.E., Jinks-Robertson S.
      Gene 98:141-145(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
      Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
      Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Construction of LYS2 cartridges for use in genetic manipulations of Saccharomyces cerevisiae."
      Fleig U.N., Pridmore R.D., Philippsen P.
      Gene 46:237-245(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-150 AND 1209-1392.
    6. "Molecular analysis of yeast chromosome II between CMD1 and LYS2: the excision repair gene RAD16 located in this region belongs to a novel group of double-finger proteins."
      Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
      Yeast 8:397-408(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1083-1392.
      Strain: ATCC 204508 / S288c.
    7. "TKL2, a second transketolase gene of Saccharomyces cerevisiae. Cloning, sequence and deletion analysis of the gene."
      Schaaff-Gerstenschlaeger I., Mannhaupt G., Vetter I., Zimmermann F.K., Feldmann H.
      Eur. J. Biochem. 217:487-492(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-130.
      Strain: ATCC 204508 / S288c.
    8. "Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5."
      Ehmann D.E., Gehring A.M., Walsh C.T.
      Biochemistry 38:6171-6177(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHOPANTETHEINYLATION AT SER-880, IDENTIFICATION BY MASS SPECTROMETRY.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLYS2_YEAST
    AccessioniPrimary (citable) accession number: P07702
    Secondary accession number(s): D6VQB4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 148 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7430 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3