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P07702

- LYS2_YEAST

UniProt

P07702 - LYS2_YEAST

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Protein
L-aminoadipate-semialdehyde dehydrogenase
Gene
LYS2, YBR115C, YBR0910
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH.

Catalytic activityi

(S)-2-amino-6-oxohexanoate + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H.

Cofactori

Binds 1 phosphopantetheine covalently.

Pathwayi

GO - Molecular functioni

  1. L-aminoadipate-semialdehyde dehydrogenase activity Source: SGD
  2. phosphopantetheine binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. lysine biosynthetic process via aminoadipic acid Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciYEAST:YBR115C-MONOMER.
UniPathwayiUPA00033; UER00032.

Names & Taxonomyi

Protein namesi
Recommended name:
L-aminoadipate-semialdehyde dehydrogenase (EC:1.2.1.31)
Alternative name(s):
Alpha-aminoadipate reductase
Short name:
Alpha-AR
Gene namesi
Name:LYS2
Ordered Locus Names:YBR115C
ORF Names:YBR0910
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR115c.
SGDiS000000319. LYS2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13921392L-aminoadipate-semialdehyde dehydrogenase
PRO_0000193153Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei880 – 8801O-(pantetheine 4'-phosphoryl)serine

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

MaxQBiP07702.
PaxDbiP07702.
PeptideAtlasiP07702.

Expressioni

Gene expression databases

GenevestigatoriP07702.

Interactioni

Protein-protein interaction databases

BioGridi32818. 22 interactions.
DIPiDIP-6811N.
IntActiP07702. 4 interactions.
MINTiMINT-661149.
STRINGi4932.YBR115C.

Structurei

3D structure databases

ProteinModelPortaliP07702.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini848 – 91770Acyl carrier
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3320.
GeneTreeiENSGT00650000093222.
HOGENOMiHOG000191209.
KOiK00143.
OMAiDRFTMLS.
OrthoDBiEOG74TX6Z.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR010071. AA_adenyl_domain.
IPR009081. Acyl_carrier_prot-like.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR014397. L-NH2adipate-semiAld_DH_lsu.
IPR013120. Male_sterile_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR006162. PPantetheine_attach_site.
IPR010080. Thioester_reductase-like_dom.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
PF07993. NAD_binding_4. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
PIRSFiPIRSF001617. Alpha-AR. 1 hit.
SUPFAMiSSF47336. SSF47336. 1 hit.
TIGRFAMsiTIGR01733. AA-adenyl-dom. 1 hit.
TIGR03443. alpha_am_amid. 1 hit.
TIGR01746. Thioester-redct. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00455. AMP_BINDING. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07702-1 [UniParc]FASTAAdd to Basket

« Hide

MTNEKVWIEK LDNPTLSVLP HDFLRPQQEP YTKQATYSLQ LPQLDVPHDS     50
FSNKYAVALS VWAALIYRVT GDDDIVLYIA NNKILRFNIQ PTWSFNELYS 100
TINNELNKLN SIEANFSFDE LAEKIQSCQD LERTPQLFRL AFLENQDFKL 150
DEFKHHLVDF ALNLDTSNNA HVLNLIYNSL LYSNERVTIV ADQFTQYLTA 200
ALSDPSNCIT KISLITASSK DSLPDPTKNL GWCDFVGCIH DIFQDNAEAF 250
PERTCVVETP TLNSDKSRSF TYRDINRTSN IVAHYLIKTG IKRGDVVMIY 300
SSRGVDLMVC VMGVLKAGAT FSVIDPAYPP ARQTIYLGVA KPRGLIVIRA 350
AGQLDQLVED YINDELEIVS RINSIAIQEN GTIEGGKLDN GEDVLAPYDH 400
YKDTRTGVVV GPDSNPTLSF TSGSEGIPKG VLGRHFSLAY YFNWMSKRFN 450
LTENDKFTML SGIAHDPIQR DMFTPLFLGA QLYVPTQDDI GTPGRLAEWM 500
SKYGCTVTHL TPAMGQLLTA QATTPFPKLH HAFFVGDILT KRDCLRLQTL 550
AENCRIVNMY GTTETQRAVS YFEVKSKNDD PNFLKKLKDV MPAGKGMLNV 600
QLLVVNRNDR TQICGIGEIG EIYVRAGGLA EGYRGLPELN KEKFVNNWFV 650
EKDHWNYLDK DNGEPWRQFW LGPRDRLYRT GDLGRYLPNG DCECCGRADD 700
QVKIRGFRIE LGEIDTHISQ HPLVRENITL VRKNADNEPT LITFMVPRFD 750
KPDDLSKFQS DVPKEVETDP IVKGLIGYHL LSKDIRTFLK KRLASYAMPS 800
LIVVMDKLPL NPNGKVDKPK LQFPTPKQLN LVAENTVSET DDSQFTNVER 850
EVRDLWLSIL PTKPASVSPD DSFFDLGGHS ILATKMIFTL KKKLQVDLPL 900
GTIFKYPTIK AFAAEIDRIK SSGGSSQGEV VENVTANYAE DAKKLVETLP 950
SSYPSREYFV EPNSAEGKTT INVFVTGVTG FLGSYILADL LGRSPKNYSF 1000
KVFAHVRAKD EEAAFARLQK AGITYGTWNE KFASNIKVVL GDLSKSQFGL 1050
SDEKWMDLAN TVDIIIHNGA LVHWVYPYAK LRDPNVISTI NVMSLAAVGK 1100
PKFFDFVSST STLDTEYYFN LSDKLVSEGK PGILESDDLM NSASGLTGGY 1150
GQSKWAAEYI IRRAGERGLR GCIVRPGYVT GASANGSSNT DDFLLRFLKG 1200
SVQLGKIPDI ENSVNMVPVD HVARVVVATS LNPPKENELA VAQVTGHPRI 1250
LFKDYLYTLH DYGYDVEIES YSKWKKSLEA SVIDRNEENA LYPLLHMVLD 1300
NLPESTKAPE LDDRNAVASL KKDTAWTGVD WSNGIGVTPE EVGIYIAFLN 1350
KVGFLPPPTH NDKLPLPSIE LTQAQISLVA SGAGARGSSA AA 1392
Length:1,392
Mass (Da):155,346
Last modified:March 1, 1992 - v2
Checksum:iF0083A80BC6F7FB5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36287 Genomic DNA. Translation: AAA34747.1.
X66247 Genomic DNA. Translation: CAA46975.1.
X78993 Genomic DNA. Translation: CAA55617.1.
Z35984 Genomic DNA. Translation: CAA85072.1.
X73532 Genomic DNA. Translation: CAA51938.1.
BK006936 Genomic DNA. Translation: DAA07234.1.
PIRiJU0448. YGBYAD.
RefSeqiNP_009673.1. NM_001178463.1.

Genome annotation databases

EnsemblFungiiYBR115C; YBR115C; YBR115C.
GeneIDi852412.
KEGGisce:YBR115C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36287 Genomic DNA. Translation: AAA34747.1 .
X66247 Genomic DNA. Translation: CAA46975.1 .
X78993 Genomic DNA. Translation: CAA55617.1 .
Z35984 Genomic DNA. Translation: CAA85072.1 .
X73532 Genomic DNA. Translation: CAA51938.1 .
BK006936 Genomic DNA. Translation: DAA07234.1 .
PIRi JU0448. YGBYAD.
RefSeqi NP_009673.1. NM_001178463.1.

3D structure databases

ProteinModelPortali P07702.
ModBasei Search...

Protein-protein interaction databases

BioGridi 32818. 22 interactions.
DIPi DIP-6811N.
IntActi P07702. 4 interactions.
MINTi MINT-661149.
STRINGi 4932.YBR115C.

Proteomic databases

MaxQBi P07702.
PaxDbi P07702.
PeptideAtlasi P07702.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR115C ; YBR115C ; YBR115C .
GeneIDi 852412.
KEGGi sce:YBR115C.

Organism-specific databases

CYGDi YBR115c.
SGDi S000000319. LYS2.

Phylogenomic databases

eggNOGi COG3320.
GeneTreei ENSGT00650000093222.
HOGENOMi HOG000191209.
KOi K00143.
OMAi DRFTMLS.
OrthoDBi EOG74TX6Z.

Enzyme and pathway databases

UniPathwayi UPA00033 ; UER00032 .
BioCyci YEAST:YBR115C-MONOMER.

Miscellaneous databases

NextBioi 971264.

Gene expression databases

Genevestigatori P07702.

Family and domain databases

Gene3Di 1.10.1200.10. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR010071. AA_adenyl_domain.
IPR009081. Acyl_carrier_prot-like.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR014397. L-NH2adipate-semiAld_DH_lsu.
IPR013120. Male_sterile_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR006162. PPantetheine_attach_site.
IPR010080. Thioester_reductase-like_dom.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
PF07993. NAD_binding_4. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view ]
PIRSFi PIRSF001617. Alpha-AR. 1 hit.
SUPFAMi SSF47336. SSF47336. 1 hit.
TIGRFAMsi TIGR01733. AA-adenyl-dom. 1 hit.
TIGR03443. alpha_am_amid. 1 hit.
TIGR01746. Thioester-redct. 1 hit.
PROSITEi PS50075. ACP_DOMAIN. 1 hit.
PS00455. AMP_BINDING. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the LYS2 gene of Saccharomyces cerevisiae: homology to Bacillus brevis tyrocidine synthetase 1."
    Morris M.E., Jinks-Robertson S.
    Gene 98:141-145(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
    Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
    Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Construction of LYS2 cartridges for use in genetic manipulations of Saccharomyces cerevisiae."
    Fleig U.N., Pridmore R.D., Philippsen P.
    Gene 46:237-245(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-150 AND 1209-1392.
  6. "Molecular analysis of yeast chromosome II between CMD1 and LYS2: the excision repair gene RAD16 located in this region belongs to a novel group of double-finger proteins."
    Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
    Yeast 8:397-408(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1083-1392.
    Strain: ATCC 204508 / S288c.
  7. "TKL2, a second transketolase gene of Saccharomyces cerevisiae. Cloning, sequence and deletion analysis of the gene."
    Schaaff-Gerstenschlaeger I., Mannhaupt G., Vetter I., Zimmermann F.K., Feldmann H.
    Eur. J. Biochem. 217:487-492(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-130.
    Strain: ATCC 204508 / S288c.
  8. "Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5."
    Ehmann D.E., Gehring A.M., Walsh C.T.
    Biochemistry 38:6171-6177(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHOPANTETHEINYLATION AT SER-880, IDENTIFICATION BY MASS SPECTROMETRY.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLYS2_YEAST
AccessioniPrimary (citable) accession number: P07702
Secondary accession number(s): D6VQB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: March 1, 1992
Last modified: May 14, 2014
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7430 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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