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P07694

- GLNA3_PEA

UniProt

P07694 - GLNA3_PEA

Protein

Glutamine synthetase root isozyme A

Gene

GS3A

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase root isozyme A (EC:6.3.1.2)
    Alternative name(s):
    Cytosolic GS3 A
    Glutamate--ammonia ligase
    Gene namesi
    Name:GS3A
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 357357Glutamine synthetase root isozyme APRO_0000153189Add
    BLAST

    Proteomic databases

    PRIDEiP07694.

    Interactioni

    Subunit structurei

    Homooctamer.

    Structurei

    3D structure databases

    ProteinModelPortaliP07694.
    SMRiP07694. Positions 4-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07694-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSLSDLINF NLSDSTEKII AEYIWVGGSG IDIRSKARTL PGPVSDPAKL    50
    PKWNYDGSST NQAPGKDSEV ILYPQAIFKD PFRRGNNILV ICDVYTPAGE 100
    PLPTNKRYNA AKIFSHPDVA AEVPWYGIEQ EYTLLQKDIN WPLGWPIGGY 150
    PGKQGPYYCG IGADKAYGRD IVDAHYKACL FAGINISGIN GEVMPGQWEF 200
    QVGPSVGISA GDEIWAARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHA 250
    NFSTKSMREN GGYEVIKKAI EKLGLRHKEH IAAYGEGNER RLTGKHETAD 300
    INVFSWGVAN RGSSIRVGRD TEKDGKGYFE DRRPASNMDP YVVTSMIAET 350
    TILWKKP 357
    Length:357
    Mass (Da):39,288
    Last modified:April 1, 1988 - v1
    Checksum:iF50C83DBB6FADDEB
    GO

    Sequence cautioni

    The sequence AAB03492.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04763 mRNA. Translation: CAA28456.1.
    U28924 Genomic DNA. Translation: AAB03492.1. Sequence problems.
    X60832 Genomic DNA. Translation: CAA43223.1.
    PIRiB26171. AJPMQA.
    S62711.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04763 mRNA. Translation: CAA28456.1 .
    U28924 Genomic DNA. Translation: AAB03492.1 . Sequence problems.
    X60832 Genomic DNA. Translation: CAA43223.1 .
    PIRi B26171. AJPMQA.
    S62711.

    3D structure databases

    ProteinModelPortali P07694.
    SMRi P07694. Positions 4-355.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P07694.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules."
      Tingey S.V., Walker E.L., Coruzzi G.M.
      EMBO J. 6:1-9(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Sparkle.
    2. "Molecular evolution of duplicate copies of genes encoding cytosolic glutamine synthetase in Pisum sativum."
      Walker E.L., Weeden N.F., Taylor C.B., Green P., Coruzzi G.M.
      Plant Mol. Biol. 29:1111-1125(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Feltham First.
    3. "A promoter sequence involved in cell-specific expression of the pea glutamine synthetase GS3A gene in organs of transgenic tobacco and alfalfa."
      Brears T., Walker E.L., Coruzzi G.M.
      Plant J. 1:235-244(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
      Strain: cv. Sparkle.

    Entry informationi

    Entry nameiGLNA3_PEA
    AccessioniPrimary (citable) accession number: P07694
    Secondary accession number(s): Q43065
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In pea there are distinct isozymes in leaves, roots and nodules.
    Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3