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P07694 (GLNA3_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase root isozyme A

EC=6.3.1.2
Alternative name(s):
Cytosolic GS3 A
Glutamate--ammonia ligase
Gene names
Name:GS3A
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Cytoplasm.

Miscellaneous

In pea there are distinct isozymes in leaves, roots and nodules.

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Sequence similarities

Belongs to the glutamine synthetase family.

Sequence caution

The sequence AAB03492.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processNitrogen fixation
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Glutamine synthetase root isozyme A
PRO_0000153189

Sequences

Sequence LengthMass (Da)Tools
P07694 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: F50C83DBB6FADDEB

FASTA35739,288
        10         20         30         40         50         60 
MSSLSDLINF NLSDSTEKII AEYIWVGGSG IDIRSKARTL PGPVSDPAKL PKWNYDGSST 

        70         80         90        100        110        120 
NQAPGKDSEV ILYPQAIFKD PFRRGNNILV ICDVYTPAGE PLPTNKRYNA AKIFSHPDVA 

       130        140        150        160        170        180 
AEVPWYGIEQ EYTLLQKDIN WPLGWPIGGY PGKQGPYYCG IGADKAYGRD IVDAHYKACL 

       190        200        210        220        230        240 
FAGINISGIN GEVMPGQWEF QVGPSVGISA GDEIWAARYI LERITEIAGV VVSFDPKPIP 

       250        260        270        280        290        300 
GDWNGAGAHA NFSTKSMREN GGYEVIKKAI EKLGLRHKEH IAAYGEGNER RLTGKHETAD 

       310        320        330        340        350 
INVFSWGVAN RGSSIRVGRD TEKDGKGYFE DRRPASNMDP YVVTSMIAET TILWKKP 

« Hide

References

[1]"Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules."
Tingey S.V., Walker E.L., Coruzzi G.M.
EMBO J. 6:1-9(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Sparkle.
[2]"Molecular evolution of duplicate copies of genes encoding cytosolic glutamine synthetase in Pisum sativum."
Walker E.L., Weeden N.F., Taylor C.B., Green P., Coruzzi G.M.
Plant Mol. Biol. 29:1111-1125(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Feltham First.
[3]"A promoter sequence involved in cell-specific expression of the pea glutamine synthetase GS3A gene in organs of transgenic tobacco and alfalfa."
Brears T., Walker E.L., Coruzzi G.M.
Plant J. 1:235-244(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
Strain: cv. Sparkle.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04763 mRNA. Translation: CAA28456.1.
U28924 Genomic DNA. Translation: AAB03492.1. Sequence problems.
X60832 Genomic DNA. Translation: CAA43223.1.
PIRAJPMQA. B26171.
S62711.

3D structure databases

ProteinModelPortalP07694.
SMRP07694. Positions 4-355.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP07694.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA3_PEA
AccessionPrimary (citable) accession number: P07694
Secondary accession number(s): Q43065
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families