P07689 (RBS3_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribulose bisphosphate carboxylase small chain 3A, chloroplastic
Short name=RuBisCO small subunit 3A
EC=4.1.1.39

Gene names

Name:

RBCS-3A

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
Subunit structure	8 large chains + 8 small chains.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the RuBisCO small chain family.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation Photorespiration Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	photorespiration Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 57

57

Chloroplast By similarity

Chain

58 – 180

123

Ribulose bisphosphate carboxylase small chain 3A, chloroplastic

PRO_0000031542

Secondary structure

1

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180

Helix Strand Turn

Details...

Beta strand

63 – 65

3

Turn

71 – 74

4

Helix

80 – 92

13

Beta strand

96 – 104

9

Beta strand

125 – 128

4

Helix

138 – 150

13

Beta strand

154 – 161

8

Beta strand

163 – 165

3

Beta strand

167 – 175

9

Sequences

	Sequence		Length	Mass (Da)
	P07689 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 33DAD53A45C0CFE7	FASTA	180	20,231
	10 20 30 40 50 60 MASMISSSAV TTVSRASTVQ SAAVAPFGGL KSMTGFPVKK VNTDITSITS NGGRVKCMQV 70 80 90 100 110 120 WPPIGKKKFE TLSYLPPLTR DQLLKEVEYL LRKGWVPCLE FELEKGFVYR EHNKSPGYYD 130 140 150 160 170 180 GRYWTMWKLP MFGTTDASQV LKELDEVVAA YPQAFVRIIG FDNVRQVQCI SFIAHTPESY

References

[1]	"Expression dynamics of the pea rbcS multigene family and organ distribution of the transcripts." Fluhr R., Moses P., Morelli G., Coruzzi G., Chua N.-H. EMBO J. 5:2063-2071(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Progress No. 9.
[2]	"Synthesis of the small subunit of ribulose-bisphosphate carboxylase from genes cloned into plasmids containing the SP6 promoter." Anderson S., Smith S.M. Biochem. J. 240:709-715(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Identification and characterization of cryptic polyadenylation sites in the 3' region of a pea ribulose-1,5-bisphosphate carboxylase small subunit gene." Hunt A.G. DNA 7:329-336(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 154-180.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X04333 Genomic DNA. Translation: CAA27864.1.
M21375 Genomic DNA. Translation: AAA33683.2.

PIR

RKPMS3. A27874.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4HHH	X-ray	2.20	S/T/U/V	58-180	[»]

ProteinModelPortal

P07689.

SMR

P07689. Positions 58-180.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.190.10. 1 hit.

InterPro

IPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
IPR024680. RuBisCO_ssu_N.
[Graphical view]

Pfam

PF12338. RbcS. 1 hit.
PF00101. RuBisCO_small. 1 hit.
[Graphical view]

PRINTS

PR00152. RUBISCOSMALL.

SUPFAM

SSF55239. RuBisCO_small. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RBS3_PEA

Accession

Primary (citable) accession number: P07689
Secondary accession number(s): P12467

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program