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P07687

- HYEP_RAT

UniProt

P07687 - HYEP_RAT

Protein

Epoxide hydrolase 1

Gene

Ephx1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.

    Catalytic activityi

    Cis-stilbene oxide + H2O = (+)-(1R,2R)-1,2-diphenylethane-1,2-diol.

    GO - Molecular functioni

    1. cis-stilbene-oxide hydrolase activity Source: UniProtKB-EC
    2. enzyme binding Source: RGD
    3. epoxide hydrolase activity Source: RGD

    GO - Biological processi

    1. aromatic compound catabolic process Source: UniProtKB-KW
    2. cellular response to glucocorticoid stimulus Source: RGD
    3. cellular response to organic substance Source: RGD
    4. liver development Source: RGD
    5. response to toxic substance Source: UniProtKB-KW
    6. xenobiotic metabolic process Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism, Detoxification

    Enzyme and pathway databases

    SABIO-RKP07687.

    Protein family/group databases

    MEROPSiS33.971.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epoxide hydrolase 1 (EC:3.3.2.9)
    Alternative name(s):
    Epoxide hydratase
    Microsomal epoxide hydrolase
    Gene namesi
    Name:Ephx1
    Synonyms:Eph-1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 13

    Organism-specific databases

    RGDi2557. Ephx1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. intracellular membrane-bounded organelle Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 455455Epoxide hydrolase 1PRO_0000080859Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei295 – 2951Omega-N-methylated arginine1 Publication
    Modified residuei439 – 4391N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Methylation

    Proteomic databases

    PaxDbiP07687.
    PRIDEiP07687.

    PTM databases

    PhosphoSiteiP07687.

    Expressioni

    Gene expression databases

    GenevestigatoriP07687.

    Interactioni

    Protein-protein interaction databases

    IntActiP07687. 1 interaction.
    MINTiMINT-4579972.
    STRINGi10116.ENSRNOP00000004780.

    Structurei

    3D structure databases

    ProteinModelPortaliP07687.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2 – 2019Helical; Signal-anchorSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S33 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0596.
    GeneTreeiENSGT00390000002210.
    HOGENOMiHOG000132894.
    HOVERGENiHBG002366.
    InParanoidiP07687.
    KOiK01253.
    OMAiHLNMALV.
    OrthoDBiEOG7B5WZH.
    PhylomeDBiP07687.
    TreeFamiTF313813.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000639. Epox_hydrolase-like.
    IPR010497. Epoxide_hydro_N.
    IPR016292. Epoxide_hydrolase.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    PF06441. EHN. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001112. Epoxide_hydrolase. 1 hit.
    PRINTSiPR00412. EPOXHYDRLASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P07687-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWLELVLASL LGFVIYWFVS RDKEETLPLG DGWWGPGSKP SAKEDESIRP    50
    FKVETSDEEI KDLHQRIDRF RASPPLEGSR FHYGFNSNYM KKVVSYWRNE 100
    FDWRKQVEIL NQYPHFKTKI EGLDIHFIHV KPPQLPSGRT PKPLLMVHGW 150
    PGSFYEFYKI IPLLTDPKSH GLSDEHVFEV ICPSIPGYGY SEASSKKGLN 200
    SVATARIFYK LMTRLGFQKF YIQGGDWGSL ICTNMAQMVP NHVKGLHLNM 250
    AFISRSFYTM TPLLGQRFGR FLGYTEKDIE LLYPYKEKVF YSIMRESGYL 300
    HIQATKPDTV GCALNDSPVG LAAYILEKFS TWTKSEYREL EDGGLERKFS 350
    LDDLLVNIMI YWTTGTIVSS QRYYKENLGQ GIMVHKHEGM KVFVPTGFSA 400
    FPSELLHAPE KWVKVKYPKL ISYSYMERGG HFAAFEEPKL LAQDIRKFVS 450
    LAELQ 455
    Length:455
    Mass (Da):52,582
    Last modified:April 1, 1988 - v1
    Checksum:i437A15BBCD6861FA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti348 – 38942Missing in AAA41585. (PubMed:3032949)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15345
    , M15339, M15340, M15341, M15342, M15343, M15344 Genomic DNA. Translation: AAA41585.1.
    M26125 mRNA. Translation: AAA42350.1.
    BC061568 mRNA. Translation: AAH61568.1.
    PIRiA26732. A26081.
    RefSeqiNP_001029262.1. NM_001034090.3.
    NP_036976.2. NM_012844.3.
    UniGeneiRn.3603.

    Genome annotation databases

    EnsembliENSRNOT00000004780; ENSRNOP00000004780; ENSRNOG00000003515.
    GeneIDi25315.
    KEGGirno:25315.
    UCSCiRGD:2557. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15345
    , M15339 , M15340 , M15341 , M15342 , M15343 , M15344 Genomic DNA. Translation: AAA41585.1 .
    M26125 mRNA. Translation: AAA42350.1 .
    BC061568 mRNA. Translation: AAH61568.1 .
    PIRi A26732. A26081.
    RefSeqi NP_001029262.1. NM_001034090.3.
    NP_036976.2. NM_012844.3.
    UniGenei Rn.3603.

    3D structure databases

    ProteinModelPortali P07687.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P07687. 1 interaction.
    MINTi MINT-4579972.
    STRINGi 10116.ENSRNOP00000004780.

    Chemistry

    ChEMBLi CHEMBL2299.

    Protein family/group databases

    MEROPSi S33.971.

    PTM databases

    PhosphoSitei P07687.

    Proteomic databases

    PaxDbi P07687.
    PRIDEi P07687.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000004780 ; ENSRNOP00000004780 ; ENSRNOG00000003515 .
    GeneIDi 25315.
    KEGGi rno:25315.
    UCSCi RGD:2557. rat.

    Organism-specific databases

    CTDi 2052.
    RGDi 2557. Ephx1.

    Phylogenomic databases

    eggNOGi COG0596.
    GeneTreei ENSGT00390000002210.
    HOGENOMi HOG000132894.
    HOVERGENi HBG002366.
    InParanoidi P07687.
    KOi K01253.
    OMAi HLNMALV.
    OrthoDBi EOG7B5WZH.
    PhylomeDBi P07687.
    TreeFami TF313813.

    Enzyme and pathway databases

    SABIO-RK P07687.

    Miscellaneous databases

    NextBioi 606139.
    PROi P07687.

    Gene expression databases

    Genevestigatori P07687.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000639. Epox_hydrolase-like.
    IPR010497. Epoxide_hydro_N.
    IPR016292. Epoxide_hydrolase.
    [Graphical view ]
    Pfami PF00561. Abhydrolase_1. 1 hit.
    PF06441. EHN. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001112. Epoxide_hydrolase. 1 hit.
    PRINTSi PR00412. EPOXHYDRLASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure and organization of the microsomal xenobiotic epoxide hydrolase gene."
      Falany C.N., McQuiddy P., Kasper C.B.
      J. Biol. Chem. 262:5924-5930(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complementary DNA and amino acid sequence of rat liver microsomal, xenobiotic epoxide hydrolase."
      Porter T.D., Beck T.W., Kasper C.B.
      Arch. Biochem. Biophys. 248:121-129(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pituitary.
    4. Cited for: METHYLATION AT ARG-295, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiHYEP_RAT
    AccessioniPrimary (citable) accession number: P07687
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3