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Protein

Epoxide hydrolase 1

Gene

Ephx1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. May play a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids.1 Publication

Catalytic activityi

Cis-stilbene oxide + H2O = (1R,2R)-1,2-diphenylethane-1,2-diol.1 Publication
1-(4-methoxyphenyl)-N-methyl-N-((3-methyloxetan-3-yl)methyl)methanamine + H2O = 2-((((4-methoxyphenyl)methyl)(methyl)amino)methyl)-2-methylpropane-1,3-diol.1 Publication

Kineticsi

  1. KM=87.5 µM for styrene 7,8-oxide1 Publication
  2. KM=6.8 µM for 9,10-epoxystearic acid1 Publication
  1. Vmax=365 nmol/min/mg enzyme with styrene 7,8-oxide as substrate1 Publication
  2. Vmax=10 nmol/min/mg enzyme with 9,10-epoxystearic acid as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei226Nucleophile1 Publication1
Active sitei374Proton donorBy similarity1
Active sitei431Proton acceptor1 Publication1

GO - Molecular functioni

  • cis-stilbene-oxide hydrolase activity Source: UniProtKB
  • epoxide hydrolase activity Source: RGD

GO - Biological processi

  • aromatic compound catabolic process Source: UniProtKB-KW
  • cellular response to glucocorticoid stimulus Source: RGD
  • cellular response to organic substance Source: RGD
  • diol biosynthetic process Source: RGD
  • epoxide metabolic process Source: UniProtKB
  • liver development Source: RGD
  • response to toxic substance Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
Biological processAromatic hydrocarbons catabolism, Detoxification

Enzyme and pathway databases

ReactomeiR-RNO-211945 Phase I - Functionalization of compounds
SABIO-RKP07687

Protein family/group databases

ESTHERiratno-hyep Epoxide_hydrolase
MEROPSiS33.971

Names & Taxonomyi

Protein namesi
Recommended name:
Epoxide hydrolase 1 (EC:3.3.2.91 Publication)
Alternative name(s):
Epoxide hydratase
Microsomal epoxide hydrolase
Short name:
mEHCurated
Gene namesi
Name:Ephx1
Synonyms:Eph-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi2557 Ephx1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini22 – 455Cytoplasmic1 PublicationAdd BLAST434

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi226D → G, N or S: Loss of catalytic activity and loss of covalent substrate binding. 1 Publication1
Mutagenesisi388E → A: Slight decrease in catalytic activity. 1 Publication1
Mutagenesisi404E → A: Loss of catalytic activity. 1 Publication1
Mutagenesisi404E → D: Increases catalytic activity. 1 Publication1
Mutagenesisi410E → A: Slight decrease in catalytic activity. 1 Publication1
Mutagenesisi431H → Q: Loss of catalytic activity and severe reduction in substrate binding. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2299

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000808591 – 455Epoxide hydrolase 1Add BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei295Dimethylated arginine1 Publication1
Modified residuei439N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

PaxDbiP07687
PRIDEiP07687

PTM databases

iPTMnetiP07687
PhosphoSitePlusiP07687

Expressioni

Gene expression databases

BgeeiENSRNOG00000003515
GenevisibleiP07687 RN

Interactioni

Protein-protein interaction databases

IntActiP07687, 1 interactor
STRINGi10116.ENSRNOP00000004780

Structurei

3D structure databases

ProteinModelPortaliP07687
SMRiP07687
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S33 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2565 Eukaryota
COG0596 LUCA
GeneTreeiENSGT00390000002210
HOGENOMiHOG000132894
HOVERGENiHBG002366
InParanoidiP07687
KOiK01253
OMAiFSEAPHQ
OrthoDBiEOG091G0B5A
PhylomeDBiP07687
TreeFamiTF313813

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000639 Epox_hydrolase-like
IPR010497 Epoxide_hydro_N
IPR016292 Epoxide_hydrolase
PfamiView protein in Pfam
PF06441 EHN, 1 hit
PIRSFiPIRSF001112 Epoxide_hydrolase, 1 hit
PRINTSiPR00412 EPOXHYDRLASE
SUPFAMiSSF53474 SSF53474, 1 hit

Sequencei

Sequence statusi: Complete.

P07687-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLELVLASL LGFVIYWFVS RDKEETLPLG DGWWGPGSKP SAKEDESIRP
60 70 80 90 100
FKVETSDEEI KDLHQRIDRF RASPPLEGSR FHYGFNSNYM KKVVSYWRNE
110 120 130 140 150
FDWRKQVEIL NQYPHFKTKI EGLDIHFIHV KPPQLPSGRT PKPLLMVHGW
160 170 180 190 200
PGSFYEFYKI IPLLTDPKSH GLSDEHVFEV ICPSIPGYGY SEASSKKGLN
210 220 230 240 250
SVATARIFYK LMTRLGFQKF YIQGGDWGSL ICTNMAQMVP NHVKGLHLNM
260 270 280 290 300
AFISRSFYTM TPLLGQRFGR FLGYTEKDIE LLYPYKEKVF YSIMRESGYL
310 320 330 340 350
HIQATKPDTV GCALNDSPVG LAAYILEKFS TWTKSEYREL EDGGLERKFS
360 370 380 390 400
LDDLLVNIMI YWTTGTIVSS QRYYKENLGQ GIMVHKHEGM KVFVPTGFSA
410 420 430 440 450
FPSELLHAPE KWVKVKYPKL ISYSYMERGG HFAAFEEPKL LAQDIRKFVS

LAELQ
Length:455
Mass (Da):52,582
Last modified:April 1, 1988 - v1
Checksum:i437A15BBCD6861FA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti348 – 389Missing in AAA41585 (PubMed:3032949).CuratedAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15345
, M15339, M15340, M15341, M15342, M15343, M15344 Genomic DNA Translation: AAA41585.1
M26125 mRNA Translation: AAA42350.1
BC061568 mRNA Translation: AAH61568.1
PIRiA26732 A26081
RefSeqiNP_001029262.1, NM_001034090.3
NP_036976.2, NM_012844.3
UniGeneiRn.3603

Genome annotation databases

EnsembliENSRNOT00000004780; ENSRNOP00000004780; ENSRNOG00000003515
ENSRNOT00000085279; ENSRNOP00000074206; ENSRNOG00000003515
GeneIDi25315
KEGGirno:25315
UCSCiRGD:2557 rat

Similar proteinsi

Entry informationi

Entry nameiHYEP_RAT
AccessioniPrimary (citable) accession number: P07687
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 23, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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