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Protein

Glucoamylase 1

Gene
N/A
Organism
Rhizopus oryzae (Mucormycosis agent) (Rhizopus arrhizus var. delemar)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei279SubstrateBy similarity1
Active sitei336Proton acceptorPROSITE-ProRule annotation1
Active sitei339Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

BRENDAi3.2.1.3. 5365.

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.
GH15. Glycoside Hydrolase Family 15.
mycoCLAPiGLA15E_RHIOR.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase 1 (EC:3.2.1.3)
Short name:
Gluc 1
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Cleaved into the following 2 chains:
Glucoamylase 2
Short name:
Gluc 2
Glucoamylase 3
Short name:
Gluc 3
OrganismiRhizopus oryzae (Mucormycosis agent) (Rhizopus arrhizus var. delemar)
Taxonomic identifieri64495 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMucoromycotinaMucoralesMucorineaeRhizopodaceaeRhizopus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4449.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Add BLAST25
ChainiPRO_000000147226 – 604Glucoamylase 1Add BLAST579
ChainiPRO_0000001473116 – 604Glucoamylase 3Add BLAST489
ChainiPRO_0000001474159 – 604Glucoamylase 2Add BLAST446

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi122N-linked (GlcNAc...)Sequence analysis1
Glycosylationi167N-linked (GlcNAc...)Sequence analysis1
Glycosylationi230N-linked (GlcNAc...)Sequence analysis1
Glycosylationi236N-linked (GlcNAc...)Sequence analysis1
Glycosylationi564N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Interactioni

Chemistry databases

BindingDBiP07683.

Structurei

Secondary structure

1604
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 44Combined sources15
Beta strandi46 – 52Combined sources7
Beta strandi55 – 57Combined sources3
Beta strandi59 – 67Combined sources9
Beta strandi78 – 80Combined sources3
Beta strandi82 – 86Combined sources5
Beta strandi93 – 99Combined sources7
Beta strandi102 – 115Combined sources14
Beta strandi117 – 120Combined sources4
Beta strandi122 – 125Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DJMNMR-A26-131[»]
ProteinModelPortaliP07683.
SMRiP07683.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07683.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 130CBM21PROSITE-ProRule annotationAdd BLAST105

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 115Adsorption to raw starchAdd BLAST90
Regioni116 – 604Starch degradationAdd BLAST489

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated
Contains 1 CBM21 (carbohydrate binding type-21) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005036. CBM21_dom.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15/PHK.
[Graphical view]
PfamiPF03370. CBM_21. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS51159. CBM21. 1 hit.
PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07683-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLFNLPLKV SFFLVLSYFS LLVSAASIPS SASVQLDSYN YDGSTFSGKI
60 70 80 90 100
YVKNIAYSKK VTVIYADGSD NWNNNGNTIA ASYSAPISGS NYEYWTFSAS
110 120 130 140 150
INGIKEFYIK YEVSGKTYYD NNNSANYQVS TSKPTTTTAT ATTTTAPSTS
160 170 180 190 200
TTTPPSRSEP ATFPTGNSTI SSWIKKQEGI SRFAMLRNIN PPGSATGFIA
210 220 230 240 250
ASLSTAGPDY YYAWTRDAAL TSNVIVYEYN TTLSGNKTIL NVLKDYVTFS
260 270 280 290 300
VKTQSTSTVC NCLGEPKFNP DASGYTGAWG RPQNDGPAER ATTFILFADS
310 320 330 340 350
YLTQTKDASY VTGTLKPAIF KDLDYVVNVW SNGCFDLWEE VNGVHFYTLM
360 370 380 390 400
VMRKGLLLGA DFAKRNGDST RASTYSSTAS TIANKISSFW VSSNNWIQVS
410 420 430 440 450
QSVTGGVSKK GLDVSTLLAA NLGSVDDGFF TPGSEKILAT AVAVEDSFAS
460 470 480 490 500
LYPINKNLPS YLGNSIGRYP EDTYNGNGNS QGNSWFLAVT GYAELYYRAI
510 520 530 540 550
KEWIGNGGVT VSSISLPFFK KFDSSATSGK KYTVGTSDFN NLAQNIALAA
560 570 580 590 600
DRFLSTVQLH AHNNGSLAEE FDRTTGLSTG ARDLTWSHAS LITASYAKAG

APAA
Length:604
Mass (Da):65,162
Last modified:November 1, 1995 - v2
Checksum:i78421F1AAA93ADB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00049 Genomic DNA. Translation: BAA00033.1.
PIRiJP0001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00049 Genomic DNA. Translation: BAA00033.1.
PIRiJP0001.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DJMNMR-A26-131[»]
ProteinModelPortaliP07683.
SMRiP07683.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP07683.
ChEMBLiCHEMBL4449.

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.
GH15. Glycoside Hydrolase Family 15.
mycoCLAPiGLA15E_RHIOR.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.2.1.3. 5365.

Miscellaneous databases

EvolutionaryTraceiP07683.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005036. CBM21_dom.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15/PHK.
[Graphical view]
PfamiPF03370. CBM_21. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS51159. CBM21. 1 hit.
PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMYG_RHIOR
AccessioniPrimary (citable) accession number: P07683
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Rhizopus glucoamylase exists in multiple forms, Gluc 1, Gluc 2, and Gluc 3, all of which hydrolyze gelatinized starch at similar rates, but only the largest one (Gluc 1) is able to adsorb raw starch.
Glucoamylase 3 may be 110-604 instead of 116-104.

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.