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Protein

Transcriptional repressor protein KorB

Gene

korB

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

In conjunction with KorA, inhibits the transcription of the kilA, trfA and korAB operons. Is also involved in the negative control of the kilB operon.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor protein KorB
Gene namesi
Name:korB
Encoded oniPlasmid IncP-alpha RK20 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Transcriptional repressor protein KorBPRO_0000178699Add
BLAST

Proteomic databases

PRIDEiP07674.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
trfBP030522EBI-6405482,EBI-6405477

Protein-protein interaction databases

DIPiDIP-17007N.
IntActiP07674. 1 interaction.

Structurei

Secondary structure

1
358
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi141 – 1488Combined sources
Helixi154 – 16613Combined sources
Helixi171 – 1788Combined sources
Helixi182 – 1898Combined sources
Helixi190 – 1923Combined sources
Helixi196 – 2038Combined sources
Helixi210 – 22213Combined sources
Helixi224 – 2329Combined sources
Helixi240 – 25112Combined sources
Beta strandi308 – 3136Combined sources
Beta strandi316 – 3205Combined sources
Beta strandi331 – 3366Combined sources
Turni337 – 3393Combined sources
Beta strandi342 – 3465Combined sources
Helixi347 – 3493Combined sources
Beta strandi351 – 3566Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IGQX-ray1.70A/B/C/D297-358[»]
1IGUX-ray2.20A/B297-358[»]
1R71X-ray2.20A/B/C/D117-294[»]
DisProtiDP00656.
ProteinModelPortaliP07674.
SMRiP07674. Positions 137-252, 299-358.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07674.

Family & Domainsi

Sequence similaritiesi

Belongs to the ParB family.Curated

Family and domain databases

Gene3Di2.30.30.150. 1 hit.
InterProiIPR010575. KorB_C.
IPR013741. KorB_domain.
IPR004437. ParB/RepB/Spo0J.
IPR003115. ParB/Sulfiredoxin_dom.
IPR008988. Transcriptional_repressor_C.
[Graphical view]
PfamiPF08535. KorB. 1 hit.
PF06613. KorB_C. 1 hit.
PF02195. ParBc. 1 hit.
[Graphical view]
SMARTiSM00470. ParB. 1 hit.
[Graphical view]
SUPFAMiSSF110849. SSF110849. 1 hit.
SSF50037. SSF50037. 1 hit.
TIGRFAMsiTIGR00180. parB_part. 1 hit.

Sequencei

Sequence statusi: Complete.

P07674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAAQAKTTK KNTAAAAQEA AGAAQPSGLG LDSIGDLSSL LDAPAASQGG
60 70 80 90 100
SGPIELDLDL IDEDPHQPRT ADNPGFSPES IAEIGATIKE RGVKSPISVR
110 120 130 140 150
ENQEQPGRYI INHGARRYRG SKWAGKKSIP AFIDNDYNEA DQVIENLQRN
160 170 180 190 200
ELTPREIADF IGRELAKGKK KGDIAKEIGK SPAFITQHVT LLDLPEKIAD
210 220 230 240 250
AFNTGRVRDV TVVNELVTAF KKRPEEVEAW LDDDTQEITR GTVKLLREFL
260 270 280 290 300
DEKGRDPNTV DAFNGQTDAE RDAEAGDGQD GEDGDQDGKD AKEKGAKEPD
310 320 330 340 350
PDKLKKAIVQ VEHDERPARL ILNRRPPAEG YAWLKYEDDG QEFEANLADV

KLVALIEG
Length:358
Mass (Da):39,011
Last modified:April 1, 1988 - v1
Checksum:i52F49477363F2279
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00448 Genomic DNA. Translation: CAA68503.1.
X03962 Genomic DNA. Translation: CAA27598.1.
X06543 Genomic DNA. Translation: CAA29790.1.
PIRiA26837. RPECKB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00448 Genomic DNA. Translation: CAA68503.1.
X03962 Genomic DNA. Translation: CAA27598.1.
X06543 Genomic DNA. Translation: CAA29790.1.
PIRiA26837. RPECKB.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IGQX-ray1.70A/B/C/D297-358[»]
1IGUX-ray2.20A/B297-358[»]
1R71X-ray2.20A/B/C/D117-294[»]
DisProtiDP00656.
ProteinModelPortaliP07674.
SMRiP07674. Positions 137-252, 299-358.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-17007N.
IntActiP07674. 1 interaction.

Proteomic databases

PRIDEiP07674.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP07674.

Family and domain databases

Gene3Di2.30.30.150. 1 hit.
InterProiIPR010575. KorB_C.
IPR013741. KorB_domain.
IPR004437. ParB/RepB/Spo0J.
IPR003115. ParB/Sulfiredoxin_dom.
IPR008988. Transcriptional_repressor_C.
[Graphical view]
PfamiPF08535. KorB. 1 hit.
PF06613. KorB_C. 1 hit.
PF02195. ParBc. 1 hit.
[Graphical view]
SMARTiSM00470. ParB. 1 hit.
[Graphical view]
SUPFAMiSSF110849. SSF110849. 1 hit.
SSF50037. SSF50037. 1 hit.
TIGRFAMsiTIGR00180. parB_part. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the transcriptional repressor gene korB which plays a key role in regulation of the copy number of broad host range plasmid RK2."
    Theophilus B.D.B., Thomas C.M.
    Nucleic Acids Res. 15:7443-7450(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of korB, a replication control gene of broad host-range plasmid RK2."
    Kornacki J.A., Balderes P.J., Figurski D.H.
    J. Mol. Biol. 198:211-222(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The trfB region of broad host range plasmid RK2: the nucleotide sequence reveals incC and key regulatory gene trfB/korA/korD as overlapping genes."
    Thomas C.M., Smith C.A.
    Nucleic Acids Res. 14:4453-4469(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-114.
  4. "The korF region of broad-host-range plasmid RK2 encodes two polypeptides with transcriptional repressor activity."
    Jagura-Burdzy G., Ibbotson J.P., Thomas C.M.
    J. Bacteriol. 173:826-833(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 327-358.

Entry informationi

Entry nameiKORB2_ECOLX
AccessioniPrimary (citable) accession number: P07674
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: October 14, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.