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Protein

Choline O-acetyltransferase

Gene

ChAT

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.1 Publication

Caution

Catalytic activityi

Acetyl-CoA + choline = CoA + O-acetylcholine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei419Proton acceptor1 Publication1
Binding sitei534Coenzyme ABy similarity1
Binding sitei656Coenzyme ABy similarity1

GO - Molecular functioni

  • choline O-acetyltransferase activity Source: UniProtKB

GO - Biological processi

  • acetylcholine biosynthetic process Source: FlyBase
  • chemical synaptic transmission Source: UniProtKB
  • nervous system development Source: UniProtKB
  • neuromuscular synaptic transmission Source: FlyBase

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processNeurotransmitter biosynthesis

Enzyme and pathway databases

ReactomeiR-DME-1483191 Synthesis of PC
R-DME-264642 Acetylcholine Neurotransmitter Release Cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Choline O-acetyltransferase1 Publication (EC:2.3.1.6)
Short name:
CHOACTaseCurated
Short name:
Choline acetylase1 Publication
Cleaved into the following 3 chains:
Choline O-acetyltransferase 67 kDa chain1 Publication
Choline O-acetyltransferase 54 kDa chain1 Publication
Choline O-acetyltransferase 13 kDa chain1 Publication
Gene namesi
Name:ChAT1 PublicationImported
Synonyms:Cha1 Publication
ORF Names:CG12345Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0000303 ChAT

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi261H → L or Q: No effect. 1 Publication1
Mutagenesisi386H → L: Loss of activity. 1 Publication1
Mutagenesisi386H → Q: No effect. 1 Publication1
Mutagenesisi419H → L or Q: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000004423? – 721Choline O-acetyltransferase 67 kDa chain
ChainiPRO_0000004421? – 584Choline O-acetyltransferase 54 kDa chain
ChainiPRO_00000044201 – 721Choline O-acetyltransferaseAdd BLAST721
ChainiPRO_0000004422585 – 721Choline O-acetyltransferase 13 kDa chainAdd BLAST137

Post-translational modificationi

The N-terminus of choline O-acetyltransferase 67 kDa and 54 kDa chains are blocked.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei584 – 585Cleavage2

Proteomic databases

PaxDbiP07668
PRIDEiP07668

Expressioni

Gene expression databases

BgeeiFBgn0000303
GenevisibleiP07668 DM

Interactioni

Subunit structurei

The 54 kDa and 13 kDa chains exist as a heterodimer.

Protein-protein interaction databases

BioGridi672551 interactor.
DIPiDIP-20557N
IntActiP07668 3 interactors.
STRINGi7227.FBpp0088397

Structurei

3D structure databases

ProteinModelPortaliP07668
SMRiP07668
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni496 – 508Coenzyme A bindingBy similarityAdd BLAST13

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3717 Eukaryota
ENOG410XNZ9 LUCA
GeneTreeiENSGT00760000119220
InParanoidiP07668
KOiK00623
OrthoDBiEOG091G038F
PhylomeDBiP07668

Family and domain databases

InterProiView protein in InterPro
IPR000542 Carn_acyl_trans
PANTHERiPTHR22589 PTHR22589, 1 hit
PfamiView protein in Pfam
PF00755 Carn_acyltransf, 1 hit
PROSITEiView protein in PROSITE
PS00439 ACYLTRANSF_C_1, 1 hit
PS00440 ACYLTRANSF_C_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P07668-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASNEASTSA AGSGPESAAL FSKLRSFSIG SGPNSPQRVV SNLRGFLTHR
60 70 80 90 100
LSNITPSDTG WKDSILSIPK KWLSTAESVD EFGFPDTLPK VPVPALDETM
110 120 130 140 150
ADYIRALEPI TTPAQLERTK ELIRQFSAPQ GIGARLHQYL LDKREAEDNW
160 170 180 190 200
AYYYWLNEMY MDIRIPLPIN SNPGMVFPPR RFKTVHDVAH FAARLLDGIL
210 220 230 240 250
SHREMLDSGE LPLERAASRE KNQPLCMAQY YRLLGSCRRP GVKQDSQFLP
260 270 280 290 300
SRERLNDEDR HVVVICRNQM YCVVLQASDR GKLSESEIAS QILYVLSDAP
310 320 330 340 350
CLPAKPVPVG LLTAEPRSTW ARDREMLQED ERNQRNLELI ETAQVVLCLD
360 370 380 390 400
EPLAGNFNAR GFTGATPTVH RAGDRDETNM AHEMIHGGGS EYNSGNRWFD
410 420 430 440 450
KTMQLIICTD GTWGLCYEHS CSEGIAVVQL LEKIYKKIEE HPDEDNGLPQ
460 470 480 490 500
HHLPPPERLE WHVGPQLQLR FAQASKSVDK CIDDLDFYVY RYQSYGKTFI
510 520 530 540 550
KSCQVSPDVY IQLALQLAHY KLYGRLVATY ESASTRRFLH GRVDCIRAAS
560 570 580 590 600
TEALEWAKAM CQGEGANVPL ESDREDEEES RKVKFSIYSK DHLRELFRCA
610 620 630 640 650
VARQTEVMVK NILGNGIDIP LLGLREASIE VTGEMHELFK DESYIISQCF
660 670 680 690 700
LLSTSQVACS TDSFMGYGPV TPRGYGCSYN PHPEQIVFCV SAFYSCEDTS
710 720
ASRYAKSLQD SLDIMRDLLQ N
Length:721
Mass (Da):81,328
Last modified:December 1, 2000 - v3
Checksum:i1867F3B8421F994E
GO
Isoform B (identifier: P07668-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     583-589: Missing.

Note: No experimental confirmation available.
Show »
Length:714
Mass (Da):80,503
Checksum:iD1341DDD1AEC7C8F
GO
Isoform C (identifier: P07668-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-85: GWKDSILSIPKKWLSTAESVDEFGFP → MERLDSVDTKEMALNGRVCGRVWIP

Show »
Length:720
Mass (Da):81,266
Checksum:i3266AED3FA211FC1
GO

Sequence cautioni

The sequence AAM75026 differs from that shown. Reason: Frameshift at position 310.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01139760 – 85GWKDS…EFGFP → MERLDSVDTKEMALNGRVCG RVWIP in isoform C. CuratedAdd BLAST26
Alternative sequenceiVSP_008316583 – 589Missing in isoform B. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63724 mRNA Translation: AAA28406.2
AE014297 Genomic DNA Translation: AAF55588.5
AE014297 Genomic DNA Translation: AAS65177.2
AY128433 mRNA Translation: AAM75026.1 Frameshift.
PIRiA24889
A36526
RefSeqiNP_477004.5, NM_057656.3 [P07668-1]
NP_996239.2, NM_206517.2 [P07668-2]
UniGeneiDm.1244

Genome annotation databases

EnsemblMetazoaiFBtr0089367; FBpp0088397; FBgn0000303 [P07668-1]
FBtr0089368; FBpp0088977; FBgn0000303 [P07668-2]
GeneIDi42249
KEGGidme:Dmel_CG12345

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCLAT_DROME
AccessioniPrimary (citable) accession number: P07668
Secondary accession number(s): Q8MQR2, Q9TXC6, Q9VE41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: December 1, 2000
Last modified: March 28, 2018
This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome