P07664 (SRYD_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serendipity locus protein delta

Gene names

Name:	Sry-delta
Synonyms:	Sry-d
ORF Names:	CG17958

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator that controls bicoid gene expression during oogenesis. Found in transcriptionally active cells. Binds to specific sites on polytene chromosomes of third instar larvae. Binds to the consensus DNA sequence 5'-YTAGAGATGGRAA-3'. Ref.9 Ref.10
Subunit structure	Binds DNA as a homodimer. N-terminal regions of the protein are required, in addition to the zinc fingers, for the specificity of chromatin-binding. Ref.9
Subcellular location	Nucleus Ref.6.
Tissue specificity	Predominantly localized to the sub- and supraoesophagal ganglia and the ventral nerve chord in the embryo, after dorsal closure. Ref.6
Developmental stage	Expressed both maternally and zygotically. Found in ovaries and abundant in early embryos. Also found in variable amounts at every stage of the life cycle. Ref.1 Ref.5 Ref.6
Sequence similarities	Contains 7 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anterior/posterior axis specification, embryo Inferred from mutant phenotype. Source: FlyBase oogenesis Inferred from genetic interaction. Source: FlyBase regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay Ref.6. Source: UniProtKB polytene chromosome interband Inferred from direct assay Ref.6. Source: UniProtKB
Molecular function	DNA binding Inferred from direct assay Ref.6. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Serendipity locus protein delta		PRO_0000047050
Regions
	Zinc finger	194 – 217	24	C2H2-type 1
	Zinc finger	223 – 245	23	C2H2-type 2
	Zinc finger	251 – 273	23	C2H2-type 3
	Zinc finger	279 – 301	23	C2H2-type 4
	Zinc finger	308 – 330	23	C2H2-type 5
	Zinc finger	337 – 359	23	C2H2-type 6
	Zinc finger	405 – 428	24	C2H2-type 7
	Motif	187 – 193	7	Nuclear localization signal Ref.7
	Compositional bias	96 – 172	77	Asp/Glu-rich (acidic)
Experimental info
	Sequence conflict	62	1	Missing in CAA26898. Ref.1
	Sequence conflict	272	1	M → S in CAA26898. Ref.1
	Sequence conflict	393	1	V → F Ref.1
	Sequence conflict	393	1	V → F Ref.5
	Sequence conflict	413	1	N → T Ref.1
	Sequence conflict	413	1	N → T Ref.5
	Sequence conflict	431 – 433	3	VLK → S Ref.1
	Sequence conflict	431 – 433	3	VLK → S Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P07664 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: 9F89FB7696ECF3F7	FASTA	433	50,028
	10 20 30 40 50 60 MDTCFFCGAV DLSDTGSSSS MRYETLSAKV PSSQKTVSLV LTHLANCIQT QLDLKPGARL 70 80 90 100 110 120 CPRCFQELSD YDTIMVNLMT TQKRLTTQLK GALKSEFEVP ESGEDILVEE VEIPQSDVET 130 140 150 160 170 180 DADAEADALF VELVKDQEES DTEIKREFVD EEEEEDDDDD DEFICEDVDV GDSEALYGKS 190 200 210 220 230 240 SDGEDRPTKK RVKQECTTCG KVYNSWYQLQ KHISEEHSKQ PNHICPICGV IRRDEEYLEL 250 260 270 280 290 300 HMNLHEGKTE KQCRYCPKSF SRPVNTLRHM RMHWDKKKYQ CEKCGLRFSQ DNLLYNHRLR 310 320 330 340 350 360 HEAEENPIIC SICNVSFKSR KTFNHHTLIH KENRPRHYCS VCPKSFTERY TLKMHMKTHE 370 380 390 400 410 420 GDVVYGVREE APADEQQVVE ELHVDVDESE AAVTVIMSDN DENSGFCLIC NTNFENKKEL 430 EHHLQFDHDV VLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and structure of the serendipity locus of Drosophila melanogaster. A densely transcribed region including a blastoderm-specific gene." Vincent A., Colot H.V., Rosbash M. J. Mol. Biol. 186:149-166(1985) [PubMed: 3935797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE. Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Drosophila maternal and embryo mRNAs transcribed from a single transcription unit use alternate combinations of exons." Vincent A., O'Connell P., Gray M.R., Rosbash M. EMBO J. 3:1003-1013(1984) [PubMed: 6329730] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 339-433, DEVELOPMENTAL STAGE.
[6]	"The closely related Drosophila sry beta and sry delta zinc finger proteins show differential embryonic expression and distinct patterns of binding sites on polytene chromosomes." Payre F., Noselli S., Lefrere V., Vincent A. Development 110:141-149(1990) [PubMed: 2081456] [Abstract] Cited for: DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[7]	"A Drosophila nuclear localisation signal included in an 18 amino acid fragment from the serendipity delta zinc finger protein." Noselli S., Vincent A. FEBS Lett. 280:167-170(1991) [PubMed: 1849091] [Abstract] Cited for: NUCLEAR LOCALIZATION SIGNAL.
[8]	"Genomic targets of the serendipity beta and delta zinc finger proteins and their respective DNA recognition sites." Payre F., Vincent A. EMBO J. 10:2533-2541(1991) [PubMed: 1868833] [Abstract] Cited for: DNA-BINDING SPECIFICITY.
[9]	"Two types of zinc fingers are required for dimerization of the serendipity delta transcriptional activator." Payre F., Buono P., Vanzo N., Vincent A. Mol. Cell. Biol. 17:3137-3145(1997) [PubMed: 9154812] [Abstract] Cited for: FUNCTION, DNA-BINDING, HOMODIMERIZATION.
[10]	"Transcriptional control of Drosophila bicoid by Serendipity delta: cooperative binding sites, promoter context, and co-evolution." Ruez C., Payre F., Vincent A. Mech. Dev. 78:125-134(1998) [PubMed: 9858707] [Abstract] Cited for: FUNCTION, DNA-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03121 Genomic DNA. Translation: CAA26898.1. AE014297 Genomic DNA. Translation: AAF57000.1. AY069691 mRNA. Translation: AAL39836.1. X00555 Genomic DNA. Translation: CAA25221.1.
PIR	C23351.
RefSeq	NP_524581.1. NM_079842.2.
UniGene	Dm.1914.
3D structure databases
ProteinModelPortal	P07664.
SMR	P07664. Positions 192-430.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07664. 1 interaction.
STRING	P07664.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0085558; FBpp0084924; FBgn0003512.
GeneID	43572.
KEGG	dme:Dmel_CG17958.
NMPDR	fig|7227.3.peg.15373.
Organism-specific databases
CTD	43572.
FlyBase	FBgn0003512. Sry-delta.
Phylogenomic databases
eggNOG	inNOG06561.
GeneTree	EMGT00050000001803.
InParanoid	P07664.
OMA	PIICSIC.
OrthoDB	EOG4BZKHZ.
PhylomeDB	P07664.
Gene expression databases
Bgee	P07664.
GermOnline	CG17958. Drosophila melanogaster.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 3 hits.
Pfam	PF00096. zf-C2H2. 4 hits. [Graphical view]
SMART	SM00355. ZnF_C2H2. 7 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 6 hits. PS50157. ZINC_FINGER_C2H2_2. 6 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	834622.

Entry information

Entry name

SRYD_DROME

Accession

Primary (citable) accession number: P07664
Secondary accession number(s): Q9VAB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	August 16, 2005
Last modified:	January 25, 2012
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project