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P07659 (RPOL_BPT3) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase

EC=2.7.7.6
Gene names
Name:1
OrganismEnterobacteria phage T3 (Bacteriophage T3)
Taxonomic identifier10759 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7likevirus
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length884 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sequence similarities

Belongs to the phage and mitochondrial RNA polymerase family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
   Molecular functionNucleotidyltransferase
Transferase
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: InterPro

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 884884DNA-directed RNA polymerase
PRO_0000087748

Sites

Active site5381 By similarity
Active site6321 By similarity
Active site8131 By similarity

Sequences

Sequence LengthMass (Da)Tools
P07659 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: D589E02D879ADA45

FASTA88498,790
        10         20         30         40         50         60 
MNIIENIEKN DFSEIELAAI PFNTLADHYG SALAKEQLAL EHESYELGER RFLKMLERQA 

        70         80         90        100        110        120 
KAGEIADNAA AKPLLATLLP KLTTRIVEWL EEYASKKGRK PSAYAPLQLL KPEASAFITL 

       130        140        150        160        170        180 
KVILASLTST NMTTIQAAAG MLGKAIEDEA RFGRIRDLEA KHFKKHVEEQ LNKRHGQVYK 

       190        200        210        220        230        240 
KAFMQVVEAD MIGRGLLGGE AWSSWDKETT MHVGIRLIEM LIESTGLVEL QRHNAGNAGS 

       250        260        270        280        290        300 
DHEALQLAQE YVDVLAKRAG ALAGISPMFQ PCVVPPKPWV AITGGGYWAN GRRPLALVRT 

       310        320        330        340        350        360 
HSKKGLMRYE DVYMPEVYKA VNLAQNTAWK INKKVLAVVN EIVNWKNCPV ADIPSLERQE 

       370        380        390        400        410        420 
LPPKPDDIDT NEAALKEWKK AAAGIYRLDK ARVSRRISLE FMLEQANKFA SKKAIWFPYN 

       430        440        450        460        470        480 
MDWRGRVYAV PMFNPQGNDM TKGLLTLAKG KPIGEEGFYW LKIHGANCAG VDKVPFPERI 

       490        500        510        520        530        540 
AFIEKHVDDI LACAKDPINN TWWAEQDSPF CFLAFCFEYA GVTHHGLSYN CSLPLAFDGS 

       550        560        570        580        590        600 
CSGIQHFSAM LRDEVGGRAV NLLPSETVQD IYGIVAQKVN EILKQDAING TPNEMITVTD 

       610        620        630        640        650        660 
KDTGEISEKL KLGTSTLAQQ WLAYGVTRSV TKRSVMTLAY GSKEFGFRQQ VLDDTIQPAI 

       670        680        690        700        710        720 
DSGKGLMFTQ PNQAAGYMAK LIWDAVSVTV VAAVEAMNWL KSAAKLLAAE VKDKKTKEIL 

       730        740        750        760        770        780 
RHRCAVHWTT PDGFPVWQEY RKPLQKRLDM IFLGQFRLQP TINTLKDSGI DAHKQESGIA 

       790        800        810        820        830        840 
PNFVHSQDGS HLRMTVVYAH EKYGIESFAL IHDSFGTIPA DAGKLFKAVR ETMVITYENN 

       850        860        870        880 
DVLADFYSQF ADQLHETQLD KMPPLPKKGN LNLQDILKSD FAFA 

« Hide

References

[1]"Sequence and analysis of the gene for bacteriophage T3 RNA polymerase."
McGraw N.J., Bailey J.N., Cleaves G.R., Dembinski D.R., Gocke C.R., Joliffe L.K., Macwright R.S., McAllister W.T.
Nucleic Acids Res. 13:6753-6766(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Hausmann.
[2]"Sequence of a conditionally essential region of bacteriophage T3, including the primary origin of DNA replication."
Schmitt M.P., Beck P.J., Kearney C.A., Spence J.L., Digiovanni D., Condreay J.P., Molineux I.J.
J. Mol. Biol. 193:479-495(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 855-884.
Strain: Luria.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X05031 Genomic DNA. Translation: CAA28696.1.
X17255 Genomic DNA. Translation: CAA35121.1.
X02981 Genomic DNA. Translation: CAA26719.1.
PIRRNBPT3. A29060.
RefSeqNP_523301.1. NC_003298.1.

3D structure databases

ProteinModelPortalP07659.
SMRP07659. Positions 6-884.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL4854.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID927437.

Family and domain databases

Gene3D1.10.1320.10. 1 hit.
1.10.287.260. 1 hit.
InterProIPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
IPR029262. RPOL_N.
[Graphical view]
PANTHERPTHR10102. PTHR10102. 1 hit.
PfamPF00940. RNA_pol. 1 hit.
PF14700. RPOL_N. 1 hit.
[Graphical view]
PROSITEPS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRPOL_BPT3
AccessionPrimary (citable) accession number: P07659
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families