P07659 (RPOL_BPT3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase
EC=2.7.7.6

Gene names

Name:

1

Organism

Enterobacteria phage T3 (Bacteriophage T3)

Taxonomic identifier

10759 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Autographivirinae › T7likevirus › unclassified T7-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	884 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Belongs to the phage and mitochondrial RNA polymerase family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 884	884	DNA-directed RNA polymerase		PRO_0000087748
Sites
	Active site	538	1	By similarity
	Active site	632	1	By similarity
	Active site	813	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07659 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: D589E02D879ADA45	FASTA	884	98,790
	10 20 30 40 50 60 MNIIENIEKN DFSEIELAAI PFNTLADHYG SALAKEQLAL EHESYELGER RFLKMLERQA 70 80 90 100 110 120 KAGEIADNAA AKPLLATLLP KLTTRIVEWL EEYASKKGRK PSAYAPLQLL KPEASAFITL 130 140 150 160 170 180 KVILASLTST NMTTIQAAAG MLGKAIEDEA RFGRIRDLEA KHFKKHVEEQ LNKRHGQVYK 190 200 210 220 230 240 KAFMQVVEAD MIGRGLLGGE AWSSWDKETT MHVGIRLIEM LIESTGLVEL QRHNAGNAGS 250 260 270 280 290 300 DHEALQLAQE YVDVLAKRAG ALAGISPMFQ PCVVPPKPWV AITGGGYWAN GRRPLALVRT 310 320 330 340 350 360 HSKKGLMRYE DVYMPEVYKA VNLAQNTAWK INKKVLAVVN EIVNWKNCPV ADIPSLERQE 370 380 390 400 410 420 LPPKPDDIDT NEAALKEWKK AAAGIYRLDK ARVSRRISLE FMLEQANKFA SKKAIWFPYN 430 440 450 460 470 480 MDWRGRVYAV PMFNPQGNDM TKGLLTLAKG KPIGEEGFYW LKIHGANCAG VDKVPFPERI 490 500 510 520 530 540 AFIEKHVDDI LACAKDPINN TWWAEQDSPF CFLAFCFEYA GVTHHGLSYN CSLPLAFDGS 550 560 570 580 590 600 CSGIQHFSAM LRDEVGGRAV NLLPSETVQD IYGIVAQKVN EILKQDAING TPNEMITVTD 610 620 630 640 650 660 KDTGEISEKL KLGTSTLAQQ WLAYGVTRSV TKRSVMTLAY GSKEFGFRQQ VLDDTIQPAI 670 680 690 700 710 720 DSGKGLMFTQ PNQAAGYMAK LIWDAVSVTV VAAVEAMNWL KSAAKLLAAE VKDKKTKEIL 730 740 750 760 770 780 RHRCAVHWTT PDGFPVWQEY RKPLQKRLDM IFLGQFRLQP TINTLKDSGI DAHKQESGIA 790 800 810 820 830 840 PNFVHSQDGS HLRMTVVYAH EKYGIESFAL IHDSFGTIPA DAGKLFKAVR ETMVITYENN 850 860 870 880 DVLADFYSQF ADQLHETQLD KMPPLPKKGN LNLQDILKSD FAFA

References

[1]	"Sequence and analysis of the gene for bacteriophage T3 RNA polymerase." McGraw N.J., Bailey J.N., Cleaves G.R., Dembinski D.R., Gocke C.R., Joliffe L.K., Macwright R.S., McAllister W.T. Nucleic Acids Res. 13:6753-6766(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Hausmann.
[2]	"Sequence of a conditionally essential region of bacteriophage T3, including the primary origin of DNA replication." Schmitt M.P., Beck P.J., Kearney C.A., Spence J.L., Digiovanni D., Condreay J.P., Molineux I.J. J. Mol. Biol. 193:479-495(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 855-884. Strain: Luria.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X05031 Genomic DNA. Translation: CAA28696.1. X17255 Genomic DNA. Translation: CAA35121.1. X02981 Genomic DNA. Translation: CAA26719.1.
PIR	RNBPT3. A29060.
RefSeq	NP_523301.1. NC_003298.1.
3D structure databases
ProteinModelPortal	P07659.
SMR	P07659. Positions 6-884.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	927437.
Phylogenomic databases
ProtClustDB	PHA0452.
Family and domain databases
Gene3D	1.10.287.260. 1 hit.
InterPro	IPR024075. DNA-dir_RNA_pol_helix_hairpin. IPR002092. DNA-dir_Rpol_phage-type. [Graphical view]
PANTHER	PTHR10102. PTHR10102. 1 hit.
Pfam	PF00940. RNA_pol. 1 hit. [Graphical view]
PROSITE	PS00900. RNA_POL_PHAGE_1. 1 hit. PS00489. RNA_POL_PHAGE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL4854.

Entry information

Entry name

RPOL_BPT3

Accession

Primary (citable) accession number: P07659

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program