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Protein

Thymidine phosphorylase

Gene

deoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The enzymes which catalyze the reversible phosphorolysis of pyrimidine nucleosides are involved in the degradation of these compounds and in their utilization as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis.

Catalytic activityi

Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.

Pathwayi: dTMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes dTMP from thymine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Thymidine phosphorylase (deoA)
  2. no protein annotated in this organism
This subpathway is part of the pathway dTMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dTMP from thymine, the pathway dTMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:DEOA-MONOMER.
ECOL316407:JW4345-MONOMER.
MetaCyc:DEOA-MONOMER.
BRENDAi2.4.2.4. 2026.
UniPathwayiUPA00578; UER00638.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine phosphorylase (EC:2.4.2.4)
Alternative name(s):
TdRPase
Gene namesi
Name:deoA
Synonyms:tpp, ttg
Ordered Locus Names:b4382, JW4345
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10219. deoA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • membrane Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3726.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000590541 – 440Thymidine phosphorylaseAdd BLAST440

Proteomic databases

EPDiP07650.
PaxDbiP07650.
PRIDEiP07650.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4262779. 9 interactors.
DIPiDIP-9426N.
IntActiP07650. 2 interactors.
STRINGi511145.b4382.

Chemistry databases

BindingDBiP07650.

Structurei

Secondary structure

1440
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 12Combined sources9
Helixi19 – 30Combined sources12
Helixi36 – 49Combined sources14
Helixi53 – 64Combined sources12
Helixi73 – 75Combined sources3
Beta strandi81 – 86Combined sources6
Helixi94 – 104Combined sources11
Beta strandi108 – 112Combined sources5
Turni117 – 119Combined sources3
Helixi123 – 127Combined sources5
Helixi139 – 149Combined sources11
Beta strandi150 – 154Combined sources5
Helixi162 – 171Combined sources10
Turni172 – 175Combined sources4
Helixi180 – 192Combined sources13
Beta strandi197 – 206Combined sources10
Beta strandi209 – 213Combined sources5
Helixi214 – 230Combined sources17
Beta strandi234 – 241Combined sources8
Beta strandi246 – 248Combined sources3
Beta strandi250 – 252Combined sources3
Helixi253 – 263Combined sources11
Helixi270 – 286Combined sources17
Helixi293 – 305Combined sources13
Helixi308 – 319Combined sources12
Helixi326 – 333Combined sources8
Beta strandi338 – 343Combined sources6
Beta strandi349 – 354Combined sources6
Helixi356 – 365Combined sources10
Turni366 – 368Combined sources3
Beta strandi371 – 375Combined sources5
Beta strandi382 – 385Combined sources4
Beta strandi392 – 394Combined sources3
Beta strandi399 – 406Combined sources8
Helixi407 – 420Combined sources14
Beta strandi421 – 426Combined sources6
Beta strandi433 – 438Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AZYX-ray3.00A/B2-440[»]
1OTPX-ray2.80A2-440[»]
1TPTX-ray2.80A2-440[»]
2TPTX-ray2.60A2-440[»]
4EADX-ray1.50A2-440[»]
4EAFX-ray1.55A2-440[»]
4LHMX-ray1.52A2-440[»]
ProteinModelPortaliP07650.
SMRiP07650.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07650.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CMW. Bacteria.
COG0213. LUCA.
HOGENOMiHOG000047313.
InParanoidiP07650.
KOiK00758.
OMAiIGNRLEV.
PhylomeDBiP07650.

Family and domain databases

Gene3Di3.40.1030.10. 1 hit.
3.90.1170.30. 1 hit.
HAMAPiMF_01628. Thymid_phosp. 1 hit.
InterProiIPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR013102. PYNP_C.
IPR018090. Pyrmidine_PPas_bac/euk.
IPR017872. Pyrmidine_PPase_CS.
IPR000053. Thymidine/pyrmidine_PPase.
IPR013465. Thymidine_Pase.
[Graphical view]
PANTHERiPTHR10515. PTHR10515. 1 hit.
PfamiPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000478. TP_PyNP. 1 hit.
SMARTiSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMiSSF47648. SSF47648. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsiTIGR02643. T_phosphoryl. 1 hit.
TIGR02644. Y_phosphoryl. 1 hit.
PROSITEiPS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07650-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLAQEIIRK KRDGHALSDE EIRFFINGIR DNTISEGQIA ALAMTIFFHD
60 70 80 90 100
MTMPERVSLT MAMRDSGTVL DWKSLHLNGP IVDKHSTGGV GDVTSLMLGP
110 120 130 140 150
MVAACGGYIP MISGRGLGHT GGTLDKLESI PGFDIFPDDN RFREIIKDVG
160 170 180 190 200
VAIIGQTSSL APADKRFYAT RDITATVDSI PLITASILAK KLAEGLDALV
210 220 230 240 250
MDVKVGSGAF MPTYELSEAL AEAIVGVANG AGVRTTALLT DMNQVLASSA
260 270 280 290 300
GNAVEVREAV QFLTGEYRNP RLFDVTMALC VEMLISGKLA KDDAEARAKL
310 320 330 340 350
QAVLDNGKAA EVFGRMVAAQ KGPTDFVENY AKYLPTAMLT KAVYADTEGF
360 370 380 390 400
VSEMDTRALG MAVVAMGGGR RQASDTIDYS VGFTDMARLG DQVDGQRPLA
410 420 430 440
VIHAKDENNW QEAAKAVKAA IKLADKAPES TPTVYRRISE
Length:440
Mass (Da):47,207
Last modified:February 1, 1995 - v3
Checksum:i1DB3083C9275D2ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97278.1.
U00096 Genomic DNA. Translation: AAC77335.1.
AP009048 Genomic DNA. Translation: BAE78371.1.
X00742 Genomic DNA. Translation: CAA25324.1.
X03224 Genomic DNA. Translation: CAA26975.1.
PIRiS56606.
RefSeqiNP_418799.1. NC_000913.3.
WP_000477807.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77335; AAC77335; b4382.
BAE78371; BAE78371; BAE78371.
GeneIDi948901.
KEGGiecj:JW4345.
eco:b4382.
PATRICi32124380. VBIEscCol129921_4530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97278.1.
U00096 Genomic DNA. Translation: AAC77335.1.
AP009048 Genomic DNA. Translation: BAE78371.1.
X00742 Genomic DNA. Translation: CAA25324.1.
X03224 Genomic DNA. Translation: CAA26975.1.
PIRiS56606.
RefSeqiNP_418799.1. NC_000913.3.
WP_000477807.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AZYX-ray3.00A/B2-440[»]
1OTPX-ray2.80A2-440[»]
1TPTX-ray2.80A2-440[»]
2TPTX-ray2.60A2-440[»]
4EADX-ray1.50A2-440[»]
4EAFX-ray1.55A2-440[»]
4LHMX-ray1.52A2-440[»]
ProteinModelPortaliP07650.
SMRiP07650.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262779. 9 interactors.
DIPiDIP-9426N.
IntActiP07650. 2 interactors.
STRINGi511145.b4382.

Chemistry databases

BindingDBiP07650.
ChEMBLiCHEMBL3726.

Proteomic databases

EPDiP07650.
PaxDbiP07650.
PRIDEiP07650.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77335; AAC77335; b4382.
BAE78371; BAE78371; BAE78371.
GeneIDi948901.
KEGGiecj:JW4345.
eco:b4382.
PATRICi32124380. VBIEscCol129921_4530.

Organism-specific databases

EchoBASEiEB0215.
EcoGeneiEG10219. deoA.

Phylogenomic databases

eggNOGiENOG4105CMW. Bacteria.
COG0213. LUCA.
HOGENOMiHOG000047313.
InParanoidiP07650.
KOiK00758.
OMAiIGNRLEV.
PhylomeDBiP07650.

Enzyme and pathway databases

UniPathwayiUPA00578; UER00638.
BioCyciEcoCyc:DEOA-MONOMER.
ECOL316407:JW4345-MONOMER.
MetaCyc:DEOA-MONOMER.
BRENDAi2.4.2.4. 2026.

Miscellaneous databases

EvolutionaryTraceiP07650.
PROiP07650.

Family and domain databases

Gene3Di3.40.1030.10. 1 hit.
3.90.1170.30. 1 hit.
HAMAPiMF_01628. Thymid_phosp. 1 hit.
InterProiIPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR013102. PYNP_C.
IPR018090. Pyrmidine_PPas_bac/euk.
IPR017872. Pyrmidine_PPase_CS.
IPR000053. Thymidine/pyrmidine_PPase.
IPR013465. Thymidine_Pase.
[Graphical view]
PANTHERiPTHR10515. PTHR10515. 1 hit.
PfamiPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000478. TP_PyNP. 1 hit.
SMARTiSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMiSSF47648. SSF47648. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsiTIGR02643. T_phosphoryl. 1 hit.
TIGR02644. Y_phosphoryl. 1 hit.
PROSITEiPS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYPH_ECOLI
AccessioniPrimary (citable) accession number: P07650
Secondary accession number(s): Q2M5T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 162 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.