Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidine phosphorylase

Gene

deoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The enzymes which catalyze the reversible phosphorolysis of pyrimidine nucleosides are involved in the degradation of these compounds and in their utilization as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis.

Catalytic activityi

Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.

Pathwayi: dTMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes dTMP from thymine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Thymidine phosphorylase (deoA)
  2. no protein annotated in this organism
This subpathway is part of the pathway dTMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dTMP from thymine, the pathway dTMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • pyrimidine nucleobase metabolic process Source: InterPro
  • pyrimidine nucleoside metabolic process Source: CAFA

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:DEOA-MONOMER
MetaCyc:DEOA-MONOMER
BRENDAi2.4.2.4 2026
UniPathwayiUPA00578; UER00638

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine phosphorylase (EC:2.4.2.4)
Alternative name(s):
TdRPase
Gene namesi
Name:deoA
Synonyms:tpp, ttg
Ordered Locus Names:b4382, JW4345
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10219 deoA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • membrane Source: UniProtKB

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3726
DrugBankiDB03462 Thymine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000590541 – 440Thymidine phosphorylaseAdd BLAST440

Proteomic databases

EPDiP07650
PaxDbiP07650
PRIDEiP07650

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi426277920 interactors.
DIPiDIP-9426N
IntActiP07650 2 interactors.
STRINGi316385.ECDH10B_4540

Chemistry databases

BindingDBiP07650

Structurei

Secondary structure

1440
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 12Combined sources9
Helixi19 – 30Combined sources12
Helixi36 – 49Combined sources14
Helixi53 – 64Combined sources12
Helixi73 – 75Combined sources3
Beta strandi81 – 86Combined sources6
Helixi94 – 104Combined sources11
Beta strandi108 – 112Combined sources5
Turni117 – 119Combined sources3
Helixi123 – 127Combined sources5
Helixi139 – 149Combined sources11
Beta strandi150 – 154Combined sources5
Helixi162 – 171Combined sources10
Turni172 – 175Combined sources4
Helixi180 – 192Combined sources13
Beta strandi197 – 206Combined sources10
Beta strandi209 – 213Combined sources5
Helixi214 – 230Combined sources17
Beta strandi234 – 241Combined sources8
Beta strandi246 – 248Combined sources3
Beta strandi250 – 252Combined sources3
Helixi253 – 263Combined sources11
Helixi270 – 286Combined sources17
Helixi293 – 305Combined sources13
Helixi308 – 319Combined sources12
Helixi326 – 333Combined sources8
Beta strandi338 – 343Combined sources6
Beta strandi349 – 354Combined sources6
Helixi356 – 365Combined sources10
Turni366 – 368Combined sources3
Beta strandi371 – 375Combined sources5
Beta strandi382 – 385Combined sources4
Beta strandi392 – 394Combined sources3
Beta strandi399 – 406Combined sources8
Helixi407 – 420Combined sources14
Beta strandi421 – 426Combined sources6
Beta strandi433 – 438Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AZYX-ray3.00A/B2-440[»]
1OTPX-ray2.80A2-440[»]
1TPTX-ray2.80A2-440[»]
2TPTX-ray2.60A2-440[»]
4EADX-ray1.50A2-440[»]
4EAFX-ray1.55A2-440[»]
4LHMX-ray1.52A2-440[»]
ProteinModelPortaliP07650
SMRiP07650
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07650

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CMW Bacteria
COG0213 LUCA
HOGENOMiHOG000047313
InParanoidiP07650
KOiK00758
OMAiVHSIGGV
PhylomeDBiP07650

Family and domain databases

Gene3Di3.40.1030.102 hits
3.90.1170.301 hit
HAMAPiMF_01628 Thymid_phosp, 1 hit
InterProiView protein in InterPro
IPR000312 Glycosyl_Trfase_fam3
IPR017459 Glycosyl_Trfase_fam3_N_dom
IPR036320 Glycosyl_Trfase_fam3_N_dom)sf
IPR035902 Nuc_phospho_transferase
IPR036566 PYNP-like_C_sf
IPR013102 PYNP_C
IPR018090 Pyrmidine_PPas_bac/euk
IPR017872 Pyrmidine_PPase_CS
IPR000053 Thymidine/pyrmidine_PPase
IPR013465 Thymidine_Pase
PANTHERiPTHR10515 PTHR10515, 1 hit
PfamiView protein in Pfam
PF02885 Glycos_trans_3N, 1 hit
PF00591 Glycos_transf_3, 1 hit
PF07831 PYNP_C, 1 hit
PIRSFiPIRSF000478 TP_PyNP, 1 hit
SMARTiView protein in SMART
SM00941 PYNP_C, 1 hit
SUPFAMiSSF47648 SSF47648, 1 hit
SSF52418 SSF52418, 1 hit
SSF54680 SSF54680, 1 hit
TIGRFAMsiTIGR02643 T_phosphoryl, 1 hit
TIGR02644 Y_phosphoryl, 1 hit
PROSITEiView protein in PROSITE
PS00647 THYMID_PHOSPHORYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

P07650-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLAQEIIRK KRDGHALSDE EIRFFINGIR DNTISEGQIA ALAMTIFFHD
60 70 80 90 100
MTMPERVSLT MAMRDSGTVL DWKSLHLNGP IVDKHSTGGV GDVTSLMLGP
110 120 130 140 150
MVAACGGYIP MISGRGLGHT GGTLDKLESI PGFDIFPDDN RFREIIKDVG
160 170 180 190 200
VAIIGQTSSL APADKRFYAT RDITATVDSI PLITASILAK KLAEGLDALV
210 220 230 240 250
MDVKVGSGAF MPTYELSEAL AEAIVGVANG AGVRTTALLT DMNQVLASSA
260 270 280 290 300
GNAVEVREAV QFLTGEYRNP RLFDVTMALC VEMLISGKLA KDDAEARAKL
310 320 330 340 350
QAVLDNGKAA EVFGRMVAAQ KGPTDFVENY AKYLPTAMLT KAVYADTEGF
360 370 380 390 400
VSEMDTRALG MAVVAMGGGR RQASDTIDYS VGFTDMARLG DQVDGQRPLA
410 420 430 440
VIHAKDENNW QEAAKAVKAA IKLADKAPES TPTVYRRISE
Length:440
Mass (Da):47,207
Last modified:February 1, 1995 - v3
Checksum:i1DB3083C9275D2ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97278.1
U00096 Genomic DNA Translation: AAC77335.1
AP009048 Genomic DNA Translation: BAE78371.1
X00742 Genomic DNA Translation: CAA25324.1
X03224 Genomic DNA Translation: CAA26975.1
PIRiS56606
RefSeqiNP_418799.1, NC_000913.3
WP_000477807.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77335; AAC77335; b4382
BAE78371; BAE78371; BAE78371
GeneIDi948901
KEGGiecj:JW4345
eco:b4382
PATRICifig|1411691.4.peg.2303

Similar proteinsi

Entry informationi

Entry nameiTYPH_ECOLI
AccessioniPrimary (citable) accession number: P07650
Secondary accession number(s): Q2M5T5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 173 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome