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P07650

- TYPH_ECOLI

UniProt

P07650 - TYPH_ECOLI

Protein

Thymidine phosphorylase

Gene

deoA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 3 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    The enzymes which catalyze the reversible phosphorolysis of pyrimidine nucleosides are involved in the degradation of these compounds and in their utilization as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis.

    Catalytic activityi

    Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.

    Pathwayi

    GO - Molecular functioni

    1. phosphorylase activity Source: InterPro
    2. pyrimidine-nucleoside phosphorylase activity Source: InterPro
    3. thymidine phosphorylase activity Source: EcoCyc

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: EcoliWiki
    2. pyrimidine nucleobase metabolic process Source: InterPro
    3. pyrimidine nucleoside metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciEcoCyc:DEOA-MONOMER.
    ECOL316407:JW4345-MONOMER.
    MetaCyc:DEOA-MONOMER.
    UniPathwayiUPA00578; UER00638.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine phosphorylase (EC:2.4.2.4)
    Alternative name(s):
    TdRPase
    Gene namesi
    Name:deoA
    Synonyms:tpp, ttg
    Ordered Locus Names:b4382, JW4345
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10219. deoA.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 440440Thymidine phosphorylasePRO_0000059054Add
    BLAST

    Proteomic databases

    PaxDbiP07650.
    PRIDEiP07650.

    Expressioni

    Gene expression databases

    GenevestigatoriP07650.

    Interactioni

    Subunit structurei

    Homodimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ygjKP425921EBI-909674,EBI-1121018

    Protein-protein interaction databases

    DIPiDIP-9426N.
    IntActiP07650. 2 interactions.
    STRINGi511145.b4382.

    Structurei

    Secondary structure

    1
    440
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 129
    Helixi19 – 3012
    Helixi36 – 4914
    Helixi53 – 6412
    Helixi73 – 753
    Beta strandi81 – 866
    Helixi94 – 10411
    Beta strandi108 – 1125
    Turni117 – 1193
    Helixi123 – 1275
    Helixi139 – 14911
    Beta strandi150 – 1545
    Helixi162 – 17110
    Turni172 – 1754
    Helixi180 – 19213
    Beta strandi197 – 20610
    Beta strandi209 – 2135
    Helixi214 – 23017
    Beta strandi234 – 2418
    Beta strandi246 – 2483
    Beta strandi250 – 2523
    Helixi253 – 26311
    Helixi270 – 28617
    Helixi293 – 30513
    Helixi308 – 31912
    Helixi326 – 3338
    Beta strandi338 – 3436
    Beta strandi349 – 3546
    Helixi356 – 36510
    Turni366 – 3683
    Beta strandi371 – 3755
    Beta strandi382 – 3854
    Beta strandi392 – 3943
    Beta strandi399 – 4068
    Helixi407 – 42014
    Beta strandi421 – 4266
    Beta strandi433 – 4386

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AZYX-ray3.00A/B2-440[»]
    1OTPX-ray2.80A2-440[»]
    1TPTX-ray2.80A2-440[»]
    2TPTX-ray2.60A2-440[»]
    4EADX-ray1.50A2-440[»]
    4EAFX-ray1.55A2-440[»]
    4LHMX-ray1.52A2-440[»]
    ProteinModelPortaliP07650.
    SMRiP07650. Positions 1-440.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07650.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0213.
    HOGENOMiHOG000047313.
    KOiK00758.
    OMAiFINGVRD.
    OrthoDBiEOG61ZTGG.
    PhylomeDBiP07650.

    Family and domain databases

    Gene3Di3.40.1030.10. 1 hit.
    3.90.1170.30. 1 hit.
    HAMAPiMF_01628. Thymid_phosp.
    InterProiIPR000312. Glycosyl_Trfase_fam3.
    IPR017459. Glycosyl_Trfase_fam3_N_dom.
    IPR013102. PYNP_C.
    IPR018090. Pyrmidine_PPas_bac/euk.
    IPR000053. Pyrmidine_PPase.
    IPR017872. Pyrmidine_PPase_CS.
    IPR013465. Thymidine_Pase.
    [Graphical view]
    PANTHERiPTHR10515. PTHR10515. 1 hit.
    PfamiPF02885. Glycos_trans_3N. 1 hit.
    PF00591. Glycos_transf_3. 1 hit.
    PF07831. PYNP_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000478. TP_PyNP. 1 hit.
    SMARTiSM00941. PYNP_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF47648. SSF47648. 1 hit.
    SSF52418. SSF52418. 1 hit.
    SSF54680. SSF54680. 1 hit.
    TIGRFAMsiTIGR02643. T_phosphoryl. 1 hit.
    TIGR02644. Y_phosphoryl. 1 hit.
    PROSITEiPS00647. THYMID_PHOSPHORYLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07650-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLAQEIIRK KRDGHALSDE EIRFFINGIR DNTISEGQIA ALAMTIFFHD    50
    MTMPERVSLT MAMRDSGTVL DWKSLHLNGP IVDKHSTGGV GDVTSLMLGP 100
    MVAACGGYIP MISGRGLGHT GGTLDKLESI PGFDIFPDDN RFREIIKDVG 150
    VAIIGQTSSL APADKRFYAT RDITATVDSI PLITASILAK KLAEGLDALV 200
    MDVKVGSGAF MPTYELSEAL AEAIVGVANG AGVRTTALLT DMNQVLASSA 250
    GNAVEVREAV QFLTGEYRNP RLFDVTMALC VEMLISGKLA KDDAEARAKL 300
    QAVLDNGKAA EVFGRMVAAQ KGPTDFVENY AKYLPTAMLT KAVYADTEGF 350
    VSEMDTRALG MAVVAMGGGR RQASDTIDYS VGFTDMARLG DQVDGQRPLA 400
    VIHAKDENNW QEAAKAVKAA IKLADKAPES TPTVYRRISE 440
    Length:440
    Mass (Da):47,207
    Last modified:February 1, 1995 - v3
    Checksum:i1DB3083C9275D2ED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14003 Genomic DNA. Translation: AAA97278.1.
    U00096 Genomic DNA. Translation: AAC77335.1.
    AP009048 Genomic DNA. Translation: BAE78371.1.
    X00742 Genomic DNA. Translation: CAA25324.1.
    X03224 Genomic DNA. Translation: CAA26975.1.
    PIRiS56606.
    RefSeqiNP_418799.1. NC_000913.3.
    YP_492512.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77335; AAC77335; b4382.
    BAE78371; BAE78371; BAE78371.
    GeneIDi12933785.
    948901.
    KEGGiecj:Y75_p4266.
    eco:b4382.
    PATRICi32124380. VBIEscCol129921_4530.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14003 Genomic DNA. Translation: AAA97278.1 .
    U00096 Genomic DNA. Translation: AAC77335.1 .
    AP009048 Genomic DNA. Translation: BAE78371.1 .
    X00742 Genomic DNA. Translation: CAA25324.1 .
    X03224 Genomic DNA. Translation: CAA26975.1 .
    PIRi S56606.
    RefSeqi NP_418799.1. NC_000913.3.
    YP_492512.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AZY X-ray 3.00 A/B 2-440 [» ]
    1OTP X-ray 2.80 A 2-440 [» ]
    1TPT X-ray 2.80 A 2-440 [» ]
    2TPT X-ray 2.60 A 2-440 [» ]
    4EAD X-ray 1.50 A 2-440 [» ]
    4EAF X-ray 1.55 A 2-440 [» ]
    4LHM X-ray 1.52 A 2-440 [» ]
    ProteinModelPortali P07650.
    SMRi P07650. Positions 1-440.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9426N.
    IntActi P07650. 2 interactions.
    STRINGi 511145.b4382.

    Chemistry

    BindingDBi P07650.
    ChEMBLi CHEMBL3726.

    Proteomic databases

    PaxDbi P07650.
    PRIDEi P07650.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77335 ; AAC77335 ; b4382 .
    BAE78371 ; BAE78371 ; BAE78371 .
    GeneIDi 12933785.
    948901.
    KEGGi ecj:Y75_p4266.
    eco:b4382.
    PATRICi 32124380. VBIEscCol129921_4530.

    Organism-specific databases

    EchoBASEi EB0215.
    EcoGenei EG10219. deoA.

    Phylogenomic databases

    eggNOGi COG0213.
    HOGENOMi HOG000047313.
    KOi K00758.
    OMAi FINGVRD.
    OrthoDBi EOG61ZTGG.
    PhylomeDBi P07650.

    Enzyme and pathway databases

    UniPathwayi UPA00578 ; UER00638 .
    BioCyci EcoCyc:DEOA-MONOMER.
    ECOL316407:JW4345-MONOMER.
    MetaCyc:DEOA-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P07650.
    PROi P07650.

    Gene expression databases

    Genevestigatori P07650.

    Family and domain databases

    Gene3Di 3.40.1030.10. 1 hit.
    3.90.1170.30. 1 hit.
    HAMAPi MF_01628. Thymid_phosp.
    InterProi IPR000312. Glycosyl_Trfase_fam3.
    IPR017459. Glycosyl_Trfase_fam3_N_dom.
    IPR013102. PYNP_C.
    IPR018090. Pyrmidine_PPas_bac/euk.
    IPR000053. Pyrmidine_PPase.
    IPR017872. Pyrmidine_PPase_CS.
    IPR013465. Thymidine_Pase.
    [Graphical view ]
    PANTHERi PTHR10515. PTHR10515. 1 hit.
    Pfami PF02885. Glycos_trans_3N. 1 hit.
    PF00591. Glycos_transf_3. 1 hit.
    PF07831. PYNP_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000478. TP_PyNP. 1 hit.
    SMARTi SM00941. PYNP_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47648. SSF47648. 1 hit.
    SSF52418. SSF52418. 1 hit.
    SSF54680. SSF54680. 1 hit.
    TIGRFAMsi TIGR02643. T_phosphoryl. 1 hit.
    TIGR02644. Y_phosphoryl. 1 hit.
    PROSITEi PS00647. THYMID_PHOSPHORYLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
      Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "The internal regulated promoter of the deo operon of Escherichia coli K-12."
      Valentin-Hansen P., Hammer K., Larsen J.E.L., Svendsen I.
      Nucleic Acids Res. 12:5211-5224(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 377-440.
      Strain: K12.
    5. "Structure and function of the intercistronic regulatory deoC-deoA element of Escherichia coli K-12."
      Valentin-Hansen P., Hammer-Jespersen K., Boetius F., Svendsen I.
      EMBO J. 3:179-183(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18.
    6. "Three-dimensional structure of thymidine phosphorylase from Escherichia coli at 2.8-A resolution."
      Walter M.R., Cook W.J., Cole L.B., Short S.A., Koszalka G.W., Krenitsky T.A., Ealick S.E.
      J. Biol. Chem. 265:14016-14022(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    7. "Structural and theoretical studies suggest domain movement produces an active conformation of thymidine phosphorylase."
      Pugmire M.J., Cook W.J., Jasanoff A., Walter M.R., Ealick S.E.
      J. Mol. Biol. 281:285-299(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

    Entry informationi

    Entry nameiTYPH_ECOLI
    AccessioniPrimary (citable) accession number: P07650
    Secondary accession number(s): Q2M5T5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 144 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3