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P07649

- TRUA_ECOLI

UniProt

P07649 - TRUA_ECOLI

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Protein
tRNA pseudouridine synthase A
Gene
truA, asuC, hisT, leuK, b2318, JW2315
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.1 Publication

Catalytic activityi

tRNA uridine(38-40) = tRNA pseudouridine(38-40).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei58 – 581Interaction with tRNA; Important for base-flipping
Active sitei60 – 601Nucleophile2 Publications
Sitei78 – 781Interaction with tRNA
Sitei110 – 1101Interaction with tRNA
Binding sitei118 – 1181Substrate
Sitei126 – 1261Interaction with tRNA
Sitei139 – 1391Interaction with tRNA

GO - Molecular functioni

  1. pseudouridine synthase activity Source: EcoCyc
  2. tRNA binding Source: EcoCyc

GO - Biological processi

  1. tRNA pseudouridine synthesis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG10454-MONOMER.
ECOL316407:JW2315-MONOMER.
MetaCyc:EG10454-MONOMER.
BRENDAi5.4.99.12. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase A (EC:5.4.99.12)
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
Short name:
PSU-I
tRNA-uridine isomerase I
Gene namesi
Name:truA
Synonyms:asuC, hisT, leuK
Ordered Locus Names:b2318, JW2315
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10454. truA.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi58 – 581R → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270tRNA pseudouridine synthase AUniRule annotation
PRO_0000057375Add
BLAST

Proteomic databases

PaxDbiP07649.
PRIDEiP07649.

Expressioni

Gene expression databases

GenevestigatoriP07649.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

DIPiDIP-11043N.
IntActiP07649. 9 interactions.
MINTiMINT-1310146.
STRINGi511145.b2318.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 189
Helixi20 – 223
Beta strandi30 – 323
Helixi35 – 4713
Beta strandi53 – 575
Beta strandi64 – 7512
Helixi80 – 8910
Beta strandi95 – 1028
Turni109 – 1124
Beta strandi115 – 1239
Beta strandi125 – 1273
Turni131 – 1344
Beta strandi135 – 1384
Helixi145 – 1528
Helixi153 – 1553
Beta strandi157 – 1604
Helixi162 – 1643
Beta strandi175 – 18612
Beta strandi189 – 1979
Helixi203 – 21513
Helixi223 – 2308
Helixi233 – 2353
Beta strandi245 – 2517
Helixi254 – 2563

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DJ0X-ray1.50A/B7-270[»]
2NQPX-ray3.50A/B/C/D1-270[»]
2NR0X-ray3.90A/B/C/D1-270[»]
2NREX-ray4.00A1-270[»]
ProteinModelPortaliP07649.
SMRiP07649. Positions 7-270.

Miscellaneous databases

EvolutionaryTraceiP07649.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni107 – 1115RNA bindingUniRule annotation
Regioni168 – 1725Interaction with tRNAUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0101.
HOGENOMiHOG000248672.
KOiK06173.
OMAiQANAFVH.
OrthoDBiEOG6Z9B4R.
PhylomeDBiP07649.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFiPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

P07649-1 [UniParc]FASTAAdd to Basket

« Hide

MSDQQQPPVY KIALGIEYDG SKYYGWQRQN EVRSVQEKLE KALSQVANEP    50
ITVFCAGRTD AGVHGTGQVV HFETTALRKD AAWTLGVNAN LPGDIAVRWV 100
KTVPDDFHAR FSATARRYRY IIYNHRLRPA VLSKGVTHFY EPLDAERMHR 150
AAQCLLGEND FTSFRAVQCQ SRTPWRNVMH INVTRHGPYV VVDIKANAFV 200
HHMVRNIVGS LMEVGAHNQP ESWIAELLAA KDRTLAAATA KAEGLYLVAV 250
DYPDRYDLPK PPMGPLFLAD 270
Length:270
Mass (Da):30,400
Last modified:April 1, 1988 - v1
Checksum:i8E97BC88C3220188
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02743 Genomic DNA. Translation: CAA26522.1.
U00096 Genomic DNA. Translation: AAC75378.1.
AP009048 Genomic DNA. Translation: BAA16175.1.
M68934 Genomic DNA. Translation: AAA23963.1.
M15542 Genomic DNA. Translation: AAA24313.1.
M15543 Genomic DNA. No translation available.
PIRiB23792. SYECZ1.
RefSeqiNP_416821.1. NC_000913.3.
YP_490560.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75378; AAC75378; b2318.
BAA16175; BAA16175; BAA16175.
GeneIDi12932365.
946793.
KEGGiecj:Y75_p2284.
eco:b2318.
PATRICi32120007. VBIEscCol129921_2413.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02743 Genomic DNA. Translation: CAA26522.1 .
U00096 Genomic DNA. Translation: AAC75378.1 .
AP009048 Genomic DNA. Translation: BAA16175.1 .
M68934 Genomic DNA. Translation: AAA23963.1 .
M15542 Genomic DNA. Translation: AAA24313.1 .
M15543 Genomic DNA. No translation available.
PIRi B23792. SYECZ1.
RefSeqi NP_416821.1. NC_000913.3.
YP_490560.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DJ0 X-ray 1.50 A/B 7-270 [» ]
2NQP X-ray 3.50 A/B/C/D 1-270 [» ]
2NR0 X-ray 3.90 A/B/C/D 1-270 [» ]
2NRE X-ray 4.00 A 1-270 [» ]
ProteinModelPortali P07649.
SMRi P07649. Positions 7-270.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-11043N.
IntActi P07649. 9 interactions.
MINTi MINT-1310146.
STRINGi 511145.b2318.

Proteomic databases

PaxDbi P07649.
PRIDEi P07649.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75378 ; AAC75378 ; b2318 .
BAA16175 ; BAA16175 ; BAA16175 .
GeneIDi 12932365.
946793.
KEGGi ecj:Y75_p2284.
eco:b2318.
PATRICi 32120007. VBIEscCol129921_2413.

Organism-specific databases

EchoBASEi EB0449.
EcoGenei EG10454. truA.

Phylogenomic databases

eggNOGi COG0101.
HOGENOMi HOG000248672.
KOi K06173.
OMAi QANAFVH.
OrthoDBi EOG6Z9B4R.
PhylomeDBi P07649.

Enzyme and pathway databases

BioCyci EcoCyc:EG10454-MONOMER.
ECOL316407:JW2315-MONOMER.
MetaCyc:EG10454-MONOMER.
BRENDAi 5.4.99.12. 2026.

Miscellaneous databases

EvolutionaryTracei P07649.
PROi P07649.

Gene expression databases

Genevestigatori P07649.

Family and domain databases

Gene3Di 3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPi MF_00171. TruA.
InterProi IPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view ]
PANTHERi PTHR11142. PTHR11142. 1 hit.
Pfami PF01416. PseudoU_synth_1. 2 hits.
[Graphical view ]
PIRSFi PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMi SSF55120. SSF55120. 1 hit.
TIGRFAMsi TIGR00071. hisT_truA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural features of the hisT operon of Escherichia coli K-12."
    Arps P.J., Marvel C.C., Rubin B.C., Tolan D.A., Penhoet E.E., Winkler M.E.
    Nucleic Acids Res. 13:5297-5315(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The hisT-purF region of the Escherichia coli K-12 chromosome. Identification of additional genes of the hisT and purF operons."
    Nonet M.L., Marvel C.C., Tolan D.R.
    J. Biol. Chem. 262:12209-12217(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 255-270.
    Strain: K12.
  6. "Structural analysis of the Escherichia coli K-12 hisT operon by using a kanamycin resistance cassette."
    Arps P.J., Winkler M.E.
    J. Bacteriol. 169:1061-1070(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10 AND 267-270.
    Strain: K12.
  7. "Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I."
    Kammen H.O., Marvel C.C., Hardy L., Penhoet E.E.
    J. Biol. Chem. 263:2255-2263(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, CHARACTERIZATION.
  8. "A conserved aspartate of tRNA pseudouridine synthase is essential for activity and a probable nucleophilic catalyst."
    Huang L., Pookanjanatavip M., Gu X., Santi D.V.
    Biochemistry 37:344-351(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITE.
  9. "The mechanism of pseudouridine synthase I as deduced from its interaction with 5-fluorouracil-tRNA."
    Gu X., Liu Y., Santi D.V.
    Proc. Natl. Acad. Sci. U.S.A. 96:14270-14275(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITE.
  10. "The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I."
    Foster P.G., Huang L., Santi D.V., Stroud R.M.
    Nat. Struct. Biol. 7:23-27(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 7-270, SUBUNIT.
  11. "How U38, 39, and 40 of many tRNAs become the targets for pseudouridylation by TruA."
    Hur S., Stroud R.M.
    Mol. Cell 26:189-203(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.9 ANGSTROMS) OF 7-270 IN COMPLEX WITH TRNA, CATALYTIC ACTIVITY, FUNCTION, SUBUNIT, MUTAGENESIS OF ARG-58.

Entry informationi

Entry nameiTRUA_ECOLI
AccessioniPrimary (citable) accession number: P07649
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 14, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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