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Protein

tRNA pseudouridine synthase A

Gene

truA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.1 Publication

Catalytic activityi

tRNA uridine(38-40) = tRNA pseudouridine(38-40).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60Nucleophile2 Publications1
Binding sitei118Substrate1

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • tRNA binding Source: EcoCyc

GO - Biological processi

  • tRNA pseudouridine synthesis Source: EcoCyc

Keywordsi

Molecular functionIsomerase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG10454-MONOMER
MetaCyc:EG10454-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase A (EC:5.4.99.12)
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
Short name:
PSU-I
tRNA-uridine isomerase I
Gene namesi
Name:truA
Synonyms:asuC, hisT, leuK
Ordered Locus Names:b2318, JW2315
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10454 truA

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi58R → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000573751 – 270tRNA pseudouridine synthase AAdd BLAST270

Proteomic databases

PaxDbiP07649
PRIDEiP07649

Interactioni

Subunit structurei

Homodimer.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei58Interaction with tRNA; Important for base-flipping1
Sitei78Interaction with tRNA1
Sitei110Interaction with tRNA1
Sitei126Interaction with tRNA1
Sitei139Interaction with tRNA1

Protein-protein interaction databases

BioGridi4261359, 63 interactors
DIPiDIP-11043N
IntActiP07649, 9 interactors
STRINGi316385.ECDH10B_2480

Structurei

Secondary structure

1270
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 18Combined sources9
Helixi20 – 22Combined sources3
Beta strandi30 – 32Combined sources3
Helixi35 – 47Combined sources13
Beta strandi53 – 57Combined sources5
Beta strandi64 – 75Combined sources12
Helixi80 – 89Combined sources10
Beta strandi95 – 102Combined sources8
Turni109 – 112Combined sources4
Beta strandi115 – 123Combined sources9
Beta strandi125 – 127Combined sources3
Turni131 – 134Combined sources4
Beta strandi135 – 138Combined sources4
Helixi145 – 152Combined sources8
Helixi153 – 155Combined sources3
Beta strandi157 – 160Combined sources4
Helixi162 – 164Combined sources3
Beta strandi175 – 186Combined sources12
Beta strandi189 – 197Combined sources9
Helixi203 – 215Combined sources13
Helixi223 – 230Combined sources8
Helixi233 – 235Combined sources3
Beta strandi245 – 251Combined sources7
Helixi254 – 256Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DJ0X-ray1.50A/B7-270[»]
2NQPX-ray3.50A/B/C/D1-270[»]
2NR0X-ray3.90A/B/C/D1-270[»]
2NREX-ray4.00A1-270[»]
ProteinModelPortaliP07649
SMRiP07649
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07649

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 111RNA binding5
Regioni168 – 172Interaction with tRNA5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DI7 Bacteria
COG0101 LUCA
HOGENOMiHOG000248672
InParanoidiP07649
KOiK06173
OMAiFLYGMVR
PhylomeDBiP07649

Family and domain databases

Gene3Di3.30.70.660, 1 hit
HAMAPiMF_00171 TruA, 1 hit
InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR001406 PsdUridine_synth_TruA
IPR020097 PsdUridine_synth_TruA_a/b_dom
IPR020095 PsdUridine_synth_TruA_C
PANTHERiPTHR11142 PTHR11142, 1 hit
PfamiView protein in Pfam
PF01416 PseudoU_synth_1, 2 hits
PIRSFiPIRSF001430 tRNA_psdUrid_synth, 1 hit
SUPFAMiSSF55120 SSF55120, 1 hit
TIGRFAMsiTIGR00071 hisT_truA, 1 hit

Sequencei

Sequence statusi: Complete.

P07649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQQQPPVY KIALGIEYDG SKYYGWQRQN EVRSVQEKLE KALSQVANEP
60 70 80 90 100
ITVFCAGRTD AGVHGTGQVV HFETTALRKD AAWTLGVNAN LPGDIAVRWV
110 120 130 140 150
KTVPDDFHAR FSATARRYRY IIYNHRLRPA VLSKGVTHFY EPLDAERMHR
160 170 180 190 200
AAQCLLGEND FTSFRAVQCQ SRTPWRNVMH INVTRHGPYV VVDIKANAFV
210 220 230 240 250
HHMVRNIVGS LMEVGAHNQP ESWIAELLAA KDRTLAAATA KAEGLYLVAV
260 270
DYPDRYDLPK PPMGPLFLAD
Length:270
Mass (Da):30,400
Last modified:April 1, 1988 - v1
Checksum:i8E97BC88C3220188
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02743 Genomic DNA Translation: CAA26522.1
U00096 Genomic DNA Translation: AAC75378.1
AP009048 Genomic DNA Translation: BAA16175.1
M68934 Genomic DNA Translation: AAA23963.1
M15542 Genomic DNA Translation: AAA24313.1
M15543 Genomic DNA No translation available.
PIRiB23792 SYECZ1
RefSeqiNP_416821.1, NC_000913.3
WP_001283586.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75378; AAC75378; b2318
BAA16175; BAA16175; BAA16175
GeneIDi946793
KEGGiecj:JW2315
eco:b2318
PATRICifig|1411691.4.peg.4416

Similar proteinsi

Entry informationi

Entry nameiTRUA_ECOLI
AccessioniPrimary (citable) accession number: P07649
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: March 28, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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