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Protein

tRNA pseudouridine synthase A

Gene

truA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.1 Publication

Catalytic activityi

tRNA uridine(38-40) = tRNA pseudouridine(38-40).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60Nucleophile2 Publications1
Binding sitei118Substrate1

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • tRNA binding Source: EcoCyc

GO - Biological processi

  • tRNA pseudouridine synthesis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG10454-MONOMER.
ECOL316407:JW2315-MONOMER.
MetaCyc:EG10454-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase A (EC:5.4.99.12)
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
Short name:
PSU-I
tRNA-uridine isomerase I
Gene namesi
Name:truA
Synonyms:asuC, hisT, leuK
Ordered Locus Names:b2318, JW2315
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10454. truA.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi58R → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000573751 – 270tRNA pseudouridine synthase AAdd BLAST270

Proteomic databases

PaxDbiP07649.
PRIDEiP07649.

Interactioni

Subunit structurei

Homodimer.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei58Interaction with tRNA; Important for base-flipping1
Sitei78Interaction with tRNA1
Sitei110Interaction with tRNA1
Sitei126Interaction with tRNA1
Sitei139Interaction with tRNA1

Protein-protein interaction databases

BioGridi4261359. 12 interactors.
DIPiDIP-11043N.
IntActiP07649. 9 interactors.
MINTiMINT-1310146.
STRINGi511145.b2318.

Structurei

Secondary structure

1270
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 18Combined sources9
Helixi20 – 22Combined sources3
Beta strandi30 – 32Combined sources3
Helixi35 – 47Combined sources13
Beta strandi53 – 57Combined sources5
Beta strandi64 – 75Combined sources12
Helixi80 – 89Combined sources10
Beta strandi95 – 102Combined sources8
Turni109 – 112Combined sources4
Beta strandi115 – 123Combined sources9
Beta strandi125 – 127Combined sources3
Turni131 – 134Combined sources4
Beta strandi135 – 138Combined sources4
Helixi145 – 152Combined sources8
Helixi153 – 155Combined sources3
Beta strandi157 – 160Combined sources4
Helixi162 – 164Combined sources3
Beta strandi175 – 186Combined sources12
Beta strandi189 – 197Combined sources9
Helixi203 – 215Combined sources13
Helixi223 – 230Combined sources8
Helixi233 – 235Combined sources3
Beta strandi245 – 251Combined sources7
Helixi254 – 256Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DJ0X-ray1.50A/B7-270[»]
2NQPX-ray3.50A/B/C/D1-270[»]
2NR0X-ray3.90A/B/C/D1-270[»]
2NREX-ray4.00A1-270[»]
ProteinModelPortaliP07649.
SMRiP07649.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07649.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 111RNA binding5
Regioni168 – 172Interaction with tRNA5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DI7. Bacteria.
COG0101. LUCA.
HOGENOMiHOG000248672.
InParanoidiP07649.
KOiK06173.
OMAiPWRNVHH.
PhylomeDBiP07649.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFiPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

P07649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQQQPPVY KIALGIEYDG SKYYGWQRQN EVRSVQEKLE KALSQVANEP
60 70 80 90 100
ITVFCAGRTD AGVHGTGQVV HFETTALRKD AAWTLGVNAN LPGDIAVRWV
110 120 130 140 150
KTVPDDFHAR FSATARRYRY IIYNHRLRPA VLSKGVTHFY EPLDAERMHR
160 170 180 190 200
AAQCLLGEND FTSFRAVQCQ SRTPWRNVMH INVTRHGPYV VVDIKANAFV
210 220 230 240 250
HHMVRNIVGS LMEVGAHNQP ESWIAELLAA KDRTLAAATA KAEGLYLVAV
260 270
DYPDRYDLPK PPMGPLFLAD
Length:270
Mass (Da):30,400
Last modified:April 1, 1988 - v1
Checksum:i8E97BC88C3220188
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02743 Genomic DNA. Translation: CAA26522.1.
U00096 Genomic DNA. Translation: AAC75378.1.
AP009048 Genomic DNA. Translation: BAA16175.1.
M68934 Genomic DNA. Translation: AAA23963.1.
M15542 Genomic DNA. Translation: AAA24313.1.
M15543 Genomic DNA. No translation available.
PIRiB23792. SYECZ1.
RefSeqiNP_416821.1. NC_000913.3.
WP_001283586.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75378; AAC75378; b2318.
BAA16175; BAA16175; BAA16175.
GeneIDi946793.
KEGGiecj:JW2315.
eco:b2318.
PATRICi32120007. VBIEscCol129921_2413.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02743 Genomic DNA. Translation: CAA26522.1.
U00096 Genomic DNA. Translation: AAC75378.1.
AP009048 Genomic DNA. Translation: BAA16175.1.
M68934 Genomic DNA. Translation: AAA23963.1.
M15542 Genomic DNA. Translation: AAA24313.1.
M15543 Genomic DNA. No translation available.
PIRiB23792. SYECZ1.
RefSeqiNP_416821.1. NC_000913.3.
WP_001283586.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DJ0X-ray1.50A/B7-270[»]
2NQPX-ray3.50A/B/C/D1-270[»]
2NR0X-ray3.90A/B/C/D1-270[»]
2NREX-ray4.00A1-270[»]
ProteinModelPortaliP07649.
SMRiP07649.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261359. 12 interactors.
DIPiDIP-11043N.
IntActiP07649. 9 interactors.
MINTiMINT-1310146.
STRINGi511145.b2318.

Proteomic databases

PaxDbiP07649.
PRIDEiP07649.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75378; AAC75378; b2318.
BAA16175; BAA16175; BAA16175.
GeneIDi946793.
KEGGiecj:JW2315.
eco:b2318.
PATRICi32120007. VBIEscCol129921_2413.

Organism-specific databases

EchoBASEiEB0449.
EcoGeneiEG10454. truA.

Phylogenomic databases

eggNOGiENOG4105DI7. Bacteria.
COG0101. LUCA.
HOGENOMiHOG000248672.
InParanoidiP07649.
KOiK06173.
OMAiPWRNVHH.
PhylomeDBiP07649.

Enzyme and pathway databases

BioCyciEcoCyc:EG10454-MONOMER.
ECOL316407:JW2315-MONOMER.
MetaCyc:EG10454-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP07649.
PROiP07649.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFiPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRUA_ECOLI
AccessioniPrimary (citable) accession number: P07649
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.