ID   KLK9_RAT                Reviewed;         259 AA.
AC   P07647;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   05-MAY-2009, entry version 73.
DE   RecName: Full=Submandibular glandular kallikrein-9;
DE            EC=3.4.21.35;
DE   AltName: Full=Tissue kallikrein;
DE   AltName: Full=S3 kallikrein;
DE   AltName: Full=Submandibular enzymatic vasoconstrictor;
DE            Short=SEV;
DE   AltName: Full=KLK-S3;
DE   Contains:
DE     RecName: Full=Submandibular glandular kallikrein-9 light chain;
DE   Contains:
DE     RecName: Full=Submandibular glandular kallikrein-9 heavy chain;
DE   Flags: Precursor;
GN   Name=Klk9; Synonyms=Klk-9, Klks3;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=86051477; PubMed=2998455; DOI=10.1021/bi00338a005;
RA   Ashley P.L., MacDonald R.J.;
RT   "Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide
RT   sequences of four distinct types including tonin.";
RL   Biochemistry 24:4512-4520(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Prostate;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   PROTEIN SEQUENCE OF 25-36 AND 112-122.
RC   TISSUE=Submandibular gland;
RX   MEDLINE=91161590; PubMed=1900513;
RA   Yamaguchi T., Carretero O.A., Scicli A.G.;
RT   "A novel serine protease with vasoconstrictor activity coded by the
RT   kallikrein gene S3.";
RL   J. Biol. Chem. 266:5011-5017(1991).
RN   [4]
RP   PROTEIN SEQUENCE OF 25-47 AND 112-135, AND CHARACTERIZATION.
RX   MEDLINE=92250562; PubMed=1315752;
RA   Moreau T., Brillard-Bourdet M., Bouhnik J., Gauthier F.;
RT   "Protein products of the rat kallikrein gene family. Substrate
RT   specificities of kallikrein rK2 (tonin) and kallikrein rK9.";
RL   J. Biol. Chem. 267:10045-10051(1992).
RN   [5]
RP   PROTEIN SEQUENCE OF 25-53 AND 112-130, AND CHARACTERIZATION.
RC   TISSUE=Submandibular gland;
RX   MEDLINE=92162030; PubMed=1536657;
RA   Berg T., Schoeyen H., Wassdal I., Hull R., Gerskowitch V.P., Toft P.;
RT   "Characterization of a new kallikrein-like enzyme (KLP-S3) of the rat
RT   submandibular gland.";
RL   Biochem. J. 281:819-828(1992).
CC   -!- FUNCTION: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds
CC       in kininogen to release Lys-bradykinin. This enzyme has a
CC       vasoconstrictor activity. KLK-9 has both a chymotrypsin-like and a
CC       trypsin-like properties.
CC   -!- CATALYTIC ACTIVITY: Preferential cleavage of Arg-|-Xaa bonds in
CC       small molecule substrates. Highly selective action to release
CC       kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of
CC       Met-|-Xaa or Leu-|-Xaa.
CC   -!- SUBUNIT: Heterodimer of a light chain and heavy chain linked by a
CC       disulfide bond.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. Kallikrein
CC       subfamily.
CC   -!- SIMILARITY: Contains 1 peptidase S1 domain.
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DR   EMBL; M11566; AAA41467.1; -; mRNA.
DR   EMBL; BC061771; AAH61771.1; -; mRNA.
DR   IPI; IPI00209517; -.
DR   PIR; D23863; D23863.
DR   RefSeq; NP_786935.1; -.
DR   UniGene; Rn.107068; -.
DR   HSSP; P00759; 1TON.
DR   SMR; P07647; 25-259.
DR   MEROPS; S01.407; -.
DR   Ensembl; ENSRNOG00000032857; Rattus norvegicus.
DR   GeneID; 292868; -.
DR   KEGG; rno:292868; -.
DR   RGD; 727805; Klks3.
DR   HOVERGEN; P07647; -.
DR   OMA; P07647; NENCAKV.
DR   BRENDA; 3.4.21.35; 248.
DR   NextBio; 634947; -.
DR   ArrayExpress; P07647; -.
DR   GermOnline; ENSRNOG00000032857; Rattus norvegicus.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0045907; P:positive regulation of vasoconstriction; IDA:RGD.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   InterPro; IPR018114; Peptidase_S1/S6_AS.
DR   InterPro; IPR001254; Peptidase_S1_S6.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; Hydrolase;
KW   Protease; Serine protease; Signal; Zymogen.
FT   SIGNAL        1     18       Probable.
FT   PROPEP       19     24       Activation peptide.
FT                                /FTId=PRO_0000028009.
FT   CHAIN        25    259       Submandibular glandular kallikrein-9.
FT                                /FTId=PRO_0000028010.
FT   CHAIN        25    111       Submandibular glandular kallikrein-9
FT                                light chain.
FT                                /FTId=PRO_0000028011.
FT   CHAIN       112    259       Submandibular glandular kallikrein-9
FT                                heavy chain.
FT                                /FTId=PRO_0000028012.
FT   DOMAIN       25    256       Peptidase S1.
FT   ACT_SITE     63     63       Charge relay system.
FT   ACT_SITE    118    118       Charge relay system.
FT   ACT_SITE    211    211       Charge relay system.
FT   CARBOHYD    106    106       N-linked (GlcNAc...) (Probable).
FT   DISULFID     31    171       By similarity.
FT   DISULFID     48     64       By similarity.
FT   DISULFID    150    217       By similarity.
FT   DISULFID    182    196       By similarity.
FT   DISULFID    207    232       By similarity.
SQ   SEQUENCE   259 AA;  28368 MW;  D167E8518BEC0791 CRC64;
     MWFLILFLAL SLGQIDAAPP GQSRVVGGYN CETNSQPWQV AVIGTTFCGG VLIDPSWVIT
     AAHCYSKNYR VLLGRNNLVK DEPFAQRRLV SQSFQHPDYI PVFMRNHTRQ RAYDHNNDLM
     LLHLSKPADI TGGVKVIDLP TEEPKVGSIC LASGWGMTNP SEMKLSHDLQ CVNIHLLSNE
     KCIETYKNIE TDVTLCAGEM DGGKDTCTGD SGGPLICDGV LQGLTSGGAT PCAKPKTPAI
     YAKLIKFTSW IKKVMKENP
//