P07647 (KLK9_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Submandibular glandular kallikrein-9
Short name=rGK-9
EC=3.4.21.35

Alternative name(s):
KLK-S3
S3 kallikrein
Submandibular enzymatic vasoconstrictor
Short name=SEV
Tissue kallikrein

Cleaved into the following 2 chains:

1. Submandibular glandular kallikrein-9 light chain
2. Submandibular glandular kallikrein-9 heavy chain

Gene names

Name:	Klk9
Synonyms:	Klk-9, Klks3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. This enzyme has a vasoconstrictor activity. KLK-9 has both a chymotrypsin-like and a trypsin-like properties.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Subunit structure	Heterodimer of a light chain and heavy chain linked by a disulfide bond.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of vasoconstriction Inferred from direct assay Ref.3. Source: RGD proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide		PRO_0000028009
	Chain	25 – 259	235	Submandibular glandular kallikrein-9		PRO_0000028010
	Chain	25 – 111	87	Submandibular glandular kallikrein-9 light chain		PRO_0000028011
	Chain	112 – 259	148	Submandibular glandular kallikrein-9 heavy chain		PRO_0000028012
Regions
	Domain	25 – 256	232	Peptidase S1
Sites
	Active site	63	1	Charge relay system
	Active site	118	1	Charge relay system
	Active site	211	1	Charge relay system
Amino acid modifications
	Glycosylation	106	1	N-linked (GlcNAc...) Probable
	Disulfide bond	31 ↔ 171		By similarity
	Disulfide bond	48 ↔ 64		By similarity
	Disulfide bond	150 ↔ 217		By similarity
	Disulfide bond	182 ↔ 196		By similarity
	Disulfide bond	207 ↔ 232		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07647 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: D167E8518BEC0791	FASTA	259	28,368
	10 20 30 40 50 60 MWFLILFLAL SLGQIDAAPP GQSRVVGGYN CETNSQPWQV AVIGTTFCGG VLIDPSWVIT 70 80 90 100 110 120 AAHCYSKNYR VLLGRNNLVK DEPFAQRRLV SQSFQHPDYI PVFMRNHTRQ RAYDHNNDLM 130 140 150 160 170 180 LLHLSKPADI TGGVKVIDLP TEEPKVGSIC LASGWGMTNP SEMKLSHDLQ CVNIHLLSNE 190 200 210 220 230 240 KCIETYKNIE TDVTLCAGEM DGGKDTCTGD SGGPLICDGV LQGLTSGGAT PCAKPKTPAI 250 YAKLIKFTSW IKKVMKENP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin." Ashley P.L., MacDonald R.J. Biochemistry 24:4512-4520(1985) [PubMed: 2998455] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[3]	"A novel serine protease with vasoconstrictor activity coded by the kallikrein gene S3." Yamaguchi T., Carretero O.A., Scicli A.G. J. Biol. Chem. 266:5011-5017(1991) [PubMed: 1900513] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-36 AND 112-122. Tissue: Submandibular gland.
[4]	"Protein products of the rat kallikrein gene family. Substrate specificities of kallikrein rK2 (tonin) and kallikrein rK9." Moreau T., Brillard-Bourdet M., Bouhnik J., Gauthier F. J. Biol. Chem. 267:10045-10051(1992) [PubMed: 1315752] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-47 AND 112-135, CHARACTERIZATION.
[5]	"Characterization of a new kallikrein-like enzyme (KLP-S3) of the rat submandibular gland." Berg T., Schoeyen H., Wassdal I., Hull R., Gerskowitch V.P., Toft P. Biochem. J. 281:819-828(1992) [PubMed: 1536657] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-53 AND 112-130, CHARACTERIZATION. Tissue: Submandibular gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11566 mRNA. Translation: AAA41467.1. BC061771 mRNA. Translation: AAH61771.1.
IPI	IPI00209517.
PIR	D23863.
RefSeq	NP_786935.1. NM_175759.2.
UniGene	Rn.224461.
3D structure databases
ProteinModelPortal	P07647.
SMR	P07647. Positions 25-259.
ModBase	Search...
Protein family/group databases
MEROPS	S01.407.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025723; ENSRNOP00000025723; ENSRNOG00000032857.
GeneID	292868.
KEGG	rno:292868.
UCSC	NM_175759. rat.
Organism-specific databases
CTD	292868.
RGD	727805. Klks3.
Phylogenomic databases
eggNOG	maNOG15788.
GeneTree	ENSGT00590000082811.
HOVERGEN	HBG013304.
InParanoid	P07647.
OMA	NENCAKV.
OrthoDB	EOG4DZ1VW.
PhylomeDB	P07647.
Gene expression databases
ArrayExpress	P07647.
Genevestigator	P07647.
GermOnline	ENSRNOG00000032857. Rattus norvegicus.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	634947.

Entry information

Entry name

KLK9_RAT

Accession

Primary (citable) accession number: P07647

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	November 16, 2011
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program