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P07647 (KLK9_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Submandibular glandular kallikrein-9

Short name=rGK-9
EC=3.4.21.35
Alternative name(s):
KLK-S3
S3 kallikrein
Submandibular enzymatic vasoconstrictor
Short name=SEV
Tissue kallikrein
Gene names
Name:Klk9
Synonyms:Klk-9, Klks3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. This enzyme has a vasoconstrictor activity. KLK-9 has both a chymotrypsin-like and a trypsin-like properties.

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Subunit structure

Heterodimer of a light chain and heavy chain linked by a disulfide bond.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Probable
Propeptide19 – 246Activation peptide
PRO_0000028009
Chain25 – 259235Submandibular glandular kallikrein-9
PRO_0000028010
Chain25 – 11187Submandibular glandular kallikrein-9 light chain
PRO_0000028011
Chain112 – 259148Submandibular glandular kallikrein-9 heavy chain
PRO_0000028012

Regions

Domain25 – 256232Peptidase S1

Sites

Active site631Charge relay system
Active site1181Charge relay system
Active site2111Charge relay system

Amino acid modifications

Glycosylation1061N-linked (GlcNAc...) Probable
Disulfide bond31 ↔ 171 By similarity
Disulfide bond48 ↔ 64 By similarity
Disulfide bond150 ↔ 217 By similarity
Disulfide bond182 ↔ 196 By similarity
Disulfide bond207 ↔ 232 By similarity

Sequences

Sequence LengthMass (Da)Tools
P07647 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: D167E8518BEC0791

FASTA25928,368
        10         20         30         40         50         60 
MWFLILFLAL SLGQIDAAPP GQSRVVGGYN CETNSQPWQV AVIGTTFCGG VLIDPSWVIT 

        70         80         90        100        110        120 
AAHCYSKNYR VLLGRNNLVK DEPFAQRRLV SQSFQHPDYI PVFMRNHTRQ RAYDHNNDLM 

       130        140        150        160        170        180 
LLHLSKPADI TGGVKVIDLP TEEPKVGSIC LASGWGMTNP SEMKLSHDLQ CVNIHLLSNE 

       190        200        210        220        230        240 
KCIETYKNIE TDVTLCAGEM DGGKDTCTGD SGGPLICDGV LQGLTSGGAT PCAKPKTPAI 

       250 
YAKLIKFTSW IKKVMKENP 

« Hide

References

« Hide 'large scale' references
[1]"Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin."
Ashley P.L., MacDonald R.J.
Biochemistry 24:4512-4520(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[3]"A novel serine protease with vasoconstrictor activity coded by the kallikrein gene S3."
Yamaguchi T., Carretero O.A., Scicli A.G.
J. Biol. Chem. 266:5011-5017(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-36 AND 112-122.
Tissue: Submandibular gland.
[4]"Protein products of the rat kallikrein gene family. Substrate specificities of kallikrein rK2 (tonin) and kallikrein rK9."
Moreau T., Brillard-Bourdet M., Bouhnik J., Gauthier F.
J. Biol. Chem. 267:10045-10051(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-47 AND 112-135, CHARACTERIZATION.
[5]"Characterization of a new kallikrein-like enzyme (KLP-S3) of the rat submandibular gland."
Berg T., Schoeyen H., Wassdal I., Hull R., Gerskowitch V.P., Toft P.
Biochem. J. 281:819-828(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-53 AND 112-130, CHARACTERIZATION.
Tissue: Submandibular gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11566 mRNA. Translation: AAA41467.1.
BC061771 mRNA. Translation: AAH61771.1.
PIRD23863.
RefSeqNP_786935.1. NM_175759.2.
UniGeneRn.224461.

3D structure databases

ProteinModelPortalP07647.
SMRP07647. Positions 25-259.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000025723.

Protein family/group databases

MEROPSS01.407.

Proteomic databases

PRIDEP07647.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000025723; ENSRNOP00000025723; ENSRNOG00000032857.
GeneID292868.
KEGGrno:292868.
UCSCRGD:727805. rat.

Organism-specific databases

CTD292868.
RGD727805. Klks3.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00750000117405.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP07647.
OMANENCAKV.
OrthoDBEOG75B84T.
PhylomeDBP07647.
TreeFamTF331065.

Gene expression databases

GenevestigatorP07647.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio634947.

Entry information

Entry nameKLK9_RAT
AccessionPrimary (citable) accession number: P07647
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 14, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries