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P07647

- KLK9_RAT

UniProt

P07647 - KLK9_RAT

Protein

Submandibular glandular kallikrein-9

Gene

Klk9

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. This enzyme has a vasoconstrictor activity. KLK-9 has both a chymotrypsin-like and a trypsin-like properties.

    Catalytic activityi

    Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei63 – 631Charge relay system
    Active sitei118 – 1181Charge relay system
    Active sitei211 – 2111Charge relay system

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. positive regulation of vasoconstriction Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.407.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Submandibular glandular kallikrein-9 (EC:3.4.21.35)
    Short name:
    rGK-9
    Alternative name(s):
    KLK-S3
    S3 kallikrein
    Submandibular enzymatic vasoconstrictor
    Short name:
    SEV
    Tissue kallikrein
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Klk9
    Synonyms:Klk-9, Klks3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi727805. Klks3.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818CuratedAdd
    BLAST
    Propeptidei19 – 246Activation peptide3 PublicationsPRO_0000028009
    Chaini25 – 259235Submandibular glandular kallikrein-9PRO_0000028010Add
    BLAST
    Chaini25 – 11187Submandibular glandular kallikrein-9 light chainPRO_0000028011Add
    BLAST
    Chaini112 – 259148Submandibular glandular kallikrein-9 heavy chainPRO_0000028012Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 171PROSITE-ProRule annotation
    Disulfide bondi48 ↔ 64PROSITE-ProRule annotation
    Glycosylationi106 – 1061N-linked (GlcNAc...)Curated
    Disulfide bondi150 ↔ 217PROSITE-ProRule annotation
    Disulfide bondi182 ↔ 196PROSITE-ProRule annotation
    Disulfide bondi207 ↔ 232PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PRIDEiP07647.

    Expressioni

    Gene expression databases

    GenevestigatoriP07647.

    Interactioni

    Subunit structurei

    Heterodimer of a light chain and heavy chain linked by a disulfide bond.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025723.

    Structurei

    3D structure databases

    ProteinModelPortaliP07647.
    SMRiP07647. Positions 25-259.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 256232Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00750000117405.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP07647.
    OMAiNENCAKV.
    OrthoDBiEOG75B84T.
    PhylomeDBiP07647.
    TreeFamiTF331065.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07647-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWFLILFLAL SLGQIDAAPP GQSRVVGGYN CETNSQPWQV AVIGTTFCGG    50
    VLIDPSWVIT AAHCYSKNYR VLLGRNNLVK DEPFAQRRLV SQSFQHPDYI 100
    PVFMRNHTRQ RAYDHNNDLM LLHLSKPADI TGGVKVIDLP TEEPKVGSIC 150
    LASGWGMTNP SEMKLSHDLQ CVNIHLLSNE KCIETYKNIE TDVTLCAGEM 200
    DGGKDTCTGD SGGPLICDGV LQGLTSGGAT PCAKPKTPAI YAKLIKFTSW 250
    IKKVMKENP 259
    Length:259
    Mass (Da):28,368
    Last modified:April 1, 1988 - v1
    Checksum:iD167E8518BEC0791
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11566 mRNA. Translation: AAA41467.1.
    BC061771 mRNA. Translation: AAH61771.1.
    PIRiD23863.
    RefSeqiNP_786935.1. NM_175759.2.
    UniGeneiRn.224461.

    Genome annotation databases

    EnsembliENSRNOT00000025723; ENSRNOP00000025723; ENSRNOG00000032857.
    GeneIDi292868.
    KEGGirno:292868.
    UCSCiRGD:727805. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11566 mRNA. Translation: AAA41467.1 .
    BC061771 mRNA. Translation: AAH61771.1 .
    PIRi D23863.
    RefSeqi NP_786935.1. NM_175759.2.
    UniGenei Rn.224461.

    3D structure databases

    ProteinModelPortali P07647.
    SMRi P07647. Positions 25-259.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000025723.

    Protein family/group databases

    MEROPSi S01.407.

    Proteomic databases

    PRIDEi P07647.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000025723 ; ENSRNOP00000025723 ; ENSRNOG00000032857 .
    GeneIDi 292868.
    KEGGi rno:292868.
    UCSCi RGD:727805. rat.

    Organism-specific databases

    CTDi 292868.
    RGDi 727805. Klks3.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00750000117405.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P07647.
    OMAi NENCAKV.
    OrthoDBi EOG75B84T.
    PhylomeDBi P07647.
    TreeFami TF331065.

    Miscellaneous databases

    NextBioi 634947.

    Gene expression databases

    Genevestigatori P07647.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin."
      Ashley P.L., MacDonald R.J.
      Biochemistry 24:4512-4520(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    3. "A novel serine protease with vasoconstrictor activity coded by the kallikrein gene S3."
      Yamaguchi T., Carretero O.A., Scicli A.G.
      J. Biol. Chem. 266:5011-5017(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-36 AND 112-122.
      Tissue: Submandibular gland.
    4. "Protein products of the rat kallikrein gene family. Substrate specificities of kallikrein rK2 (tonin) and kallikrein rK9."
      Moreau T., Brillard-Bourdet M., Bouhnik J., Gauthier F.
      J. Biol. Chem. 267:10045-10051(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-47 AND 112-135, CHARACTERIZATION.
    5. "Characterization of a new kallikrein-like enzyme (KLP-S3) of the rat submandibular gland."
      Berg T., Schoeyen H., Wassdal I., Hull R., Gerskowitch V.P., Toft P.
      Biochem. J. 281:819-828(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-53 AND 112-130, CHARACTERIZATION.
      Tissue: Submandibular gland.

    Entry informationi

    Entry nameiKLK9_RAT
    AccessioniPrimary (citable) accession number: P07647
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3