P07639 (AROB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate synthase
EC=4.2.3.4

Gene names

Name:	aroB
Ordered Locus Names:	b3389, JW3352

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate. HAMAP MF_00110
Cofactor	NAD. Ref.6 Divalent metal cation. Ref.6
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7. HAMAP MF_00110
Subunit structure	Monomer.
Subcellular location	Cytoplasm HAMAP MF_00110.
Sequence similarities	Belongs to the dehydroquinate synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	Cobalt NAD
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	3-dehydroquinate synthase HAMAP MF_00110		PRO_0000140737

Sequences

	Sequence		Length	Mass (Da)
	P07639 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: F67496010D5C9182	FASTA	362	38,881
	10 20 30 40 50 60 MERIVVTLGE RSYPITIASG LFNEPASFLP LKSGEQVMLV TNETLAPLYL DKVRGVLEQA 70 80 90 100 110 120 GVNVDSVILP DGEQYKSLAV LDTVFTALLQ KPHGRDTTLV ALGGGVVGDL TGFAAASYQR 130 140 150 160 170 180 GVRFIQVPTT LLSQVDSSVG GKTAVNHPLG KNMIGAFYQP ASVVVDLDCL KTLPPRELAS 190 200 210 220 230 240 GLAEVIKYGI ILDGAFFNWL EENLDALLRL DGPAMAYCIR RCCELKAEVV AADERETGLR 250 260 270 280 290 300 ALLNLGHTFG HAIEAEMGYG NWLHGEAVAA GMVMAARTSE RLGQFSSAET QRIITLLKRA 310 320 330 340 350 360 GLPVNGPREM SAQAYLPHML RDKKVLAGEM RLILPLAIGK SEVRSGVSHE LVLNAIADCQ SA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete amino acid sequence of 3-dehydroquinate synthase of Escherichia coli K12." Millar G., Coggins J.R. FEBS Lett. 200:11-17(1986) [PubMed: 3009224] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Characterization of three genes in the dam-containing operon of Escherichia coli." Lyngstadaas A., Lobner-Olesen A., Boye E. Mol. Gen. Genet. 247:546-554(1995) [PubMed: 7603433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The Escherichia coli dam gene is expressed as a distal gene of a new operon." Jonczyk P., Hines R., Smith D.W. Mol. Gen. Genet. 217:85-96(1989) [PubMed: 2549371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 357-362. Strain: K12.
[6]	"Dehydroquinate synthase: the role of divalent metal cations and of nicotinamide adenine dinucleotide in catalysis." Bender S.L., Mehdi S., Knowles J.R. Biochemistry 28:7555-7560(1989) [PubMed: 2514789] [Abstract] Cited for: COFACTOR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03867 Genomic DNA. Translation: CAA27495.1. Z19601 Genomic DNA. Translation: CAA79666.1. U18997 Genomic DNA. Translation: AAA58186.1. U00096 Genomic DNA. Translation: AAC76414.1. AP009048 Genomic DNA. Translation: BAE77902.1. X15162 Genomic DNA. Translation: CAA33252.1.
PIR	SYECQ. A24863.
RefSeq	NP_417848.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P07639.
SMR	P07639. Positions 1-361.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9150N.
IntAct	P07639. 5 interactions.
MINT	MINT-1261041.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003312; EBESCP00000003312; EBESCG00000002716. EBESCT00000003313; EBESCP00000003313; EBESCG00000002716. EBESCT00000016208; EBESCP00000015499; EBESCG00000015268.
GeneID	947927.
GenomeReviews	Gene locus JW3352 in contig AP009048_GR. Gene locus b3389 in contig U00096_GR.
KEGG	ecj:JW3352. eco:b3389.
PATRIC	32122210. VBIEscCol129921_3482.
Organism-specific databases
EchoBASE	EB0072.
EcoGene	EG10074. aroB.
Phylogenomic databases
eggNOG	COG0337.
GeneTree	EBGT00050000009748.
HOGENOM	HBG632303.
OMA	VMALEMS.
PhylomeDB	P07639.
ProtClustDB	PRK00002.
Enzyme and pathway databases
BioCyc	EcoCyc:AROB-MONOMER. MetaCyc:AROB-MONOMER.
BRENDA	4.2.3.4. 2026.
Gene expression databases
Genevestigator	P07639.
Family and domain databases
HAMAP	MF_00110. DHQ_synthase. [Tree]
InterPro	IPR016037. DHQ_synth_AroB. [Graphical view]
KO	K01735.
PIRSF	PIRSF001455. DHQ_synth. 1 hit.
TIGRFAMs	TIGR01357. AroB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROB_ECOLI

Accession

Primary (citable) accession number: P07639
Secondary accession number(s): Q2M754

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	January 25, 2012
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program