Reviewed, UniProtKB/Swiss-Prot P07628 (K1KB8_MOUSE)

Names and origin

Protein names

Recommended name:
    Kallikrein 1-related peptidase b8
    EC=3.4.21.35
Alternative name(s):
    Glandular kallikrein K8
      Short name=mGK-8
    Tissue kallikrein-8

Gene names

Name:	Klk1b8
Synonyms:	Klk-8, Klk8

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide Probable		PRO_0000027977
	Chain	25 – 261	237	Kallikrein 1-related peptidase b8		PRO_0000027978
Regions
	Domain	25 – 258	234	Peptidase S1
Sites
	Active site	65	1	Charge relay system
	Active site	120	1	Charge relay system
	Active site	213	1	Charge relay system
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Probable
	Disulfide bond	31 ↔ 173		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	152 ↔ 219		By similarity
	Disulfide bond	184 ↔ 198		By similarity
	Disulfide bond	209 ↔ 234		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07628-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: D0174F7093137322	FASTA	261	28,531
	10 20 30 40 50 60 MRFLILFLAL SLGGIDAAPP LQSRVVGGFN CEKNSQPWQV AVYDNKEHIC GGVLLERNWV 70 80 90 100 110 120 LTAAHCYVDQ YEVWLGKNKL FQEEPSAQHR LVSKSFPHPG FNMSLLTLKE IPPGADFSND 130 140 150 160 170 180 LMLLRLSKPA DITDAVKPIT LPTKESKLGS TCLASGWGSI TPTKWQKPDD LQCVFLKLLP 190 200 210 220 230 240 IKNCIENHNV KVTDVMLCAG EMSGGKNICK GDSGGPLICD SVLQGITSTG PIPCGKPGVP 250 260 AMYTNLIKFN SWIKDTMTKN S

References

[1]	"The sequence of a cDNA clone coding for a novel kallikrein from mouse submaxillary gland." Fahnestock M., Brundage S., Shooter E.M. Nucleic Acids Res. 14:4823-4835(1986) [PubMed: 3636812] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus." Evans B.A., Drinkwater C.C., Richards R.I. J. Biol. Chem. 262:8027-8034(1987) [PubMed: 3036794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 16-54 AND 70-122.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X03994 mRNA. Translation: CAA27630.1. M18587 Genomic DNA. Translation: AAA39347.1. M18607 Genomic DNA. Translation: AAA39348.1.
IPI	IPI00130801.
PIR	A24378.
RefSeq	NP_032483.1.
UniGene	Mm.435488
3D structure databases
HSSP	HSSP built from PDB template 1AO5 based on UniProtKB P36368.
SMR	P07628. Positions 25-261.
ModBase	Search...
Proteomic databases
PRIDE	P07628.
Genome annotation databases
Ensembl	ENSMUSG00000063089. Mus musculus. [Contig view]
GeneID	16624.
KEGG	mmu:16624.
Organism-specific databases
MGI	MGI:892018. Klk1b8.
Phylogenomic databases
HOVERGEN	P07628.
Enzyme and pathway databases
BRENDA	3.4.21.118. 244. 3.4.21.35. 244.
Gene expression databases
ArrayExpress	P07628.
Bgee	P07628.
CleanEx	MM_KLK1B8. MM_KLK8.
GermOnline	ENSMUSG00000063089. Mus musculus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	290239.
SOURCE	Search...

Entry information

Entry name

K1KB8_MOUSE

Accession

Primary (citable) accession number: P07628

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 26, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)