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Protein

Kallikrein 1-related peptidase b8

Gene

Klk1b8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay system1
Active sitei120Charge relay system1
Active sitei213Charge relay system1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.118 3474

Protein family/group databases

MEROPSiS01.067

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b8 (EC:3.4.21.35)
Alternative name(s):
Glandular kallikrein K8
Short name:
mGK-8
Tissue kallikrein-8
Gene namesi
Name:Klk1b8
Synonyms:Klk-8, Klk8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:892018 Klk1b8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18CuratedAdd BLAST18
PropeptideiPRO_000002797719 – 24Activation peptideCurated6
ChainiPRO_000002797825 – 261Kallikrein 1-related peptidase b8Add BLAST237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 173PROSITE-ProRule annotation
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Glycosylationi102N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi152 ↔ 219PROSITE-ProRule annotation
Disulfide bondi184 ↔ 198PROSITE-ProRule annotation
Disulfide bondi209 ↔ 234PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP07628
PaxDbiP07628
PRIDEiP07628

Expressioni

Gene expression databases

BgeeiENSMUSG00000063089
CleanExiMM_KLK1B8
MM_KLK8
GenevisibleiP07628 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072063

Structurei

3D structure databases

ProteinModelPortaliP07628
SMRiP07628
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 258Peptidase S1PROSITE-ProRule annotationAdd BLAST234

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00910000144067
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP07628
KOiK01325
OMAiCAGEMSG
OrthoDBiEOG091G0AH5
PhylomeDBiP07628
TreeFamiTF331065

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07628-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFLILFLAL SLGGIDAAPP LQSRVVGGFN CEKNSQPWQV AVYDNKEHIC
60 70 80 90 100
GGVLLERNWV LTAAHCYVDQ YEVWLGKNKL FQEEPSAQHR LVSKSFPHPG
110 120 130 140 150
FNMSLLTLKE IPPGADFSND LMLLRLSKPA DITDAVKPIT LPTKESKLGS
160 170 180 190 200
TCLASGWGSI TPTKWQKPDD LQCVFLKLLP IKNCIENHNV KVTDVMLCAG
210 220 230 240 250
EMSGGKNICK GDSGGPLICD SVLQGITSTG PIPCGKPGVP AMYTNLIKFN
260
SWIKDTMTKN S
Length:261
Mass (Da):28,531
Last modified:April 1, 1988 - v1
Checksum:iD0174F7093137322
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03994 mRNA Translation: CAA27630.1
M18587 Genomic DNA Translation: AAA39347.1
M18607 Genomic DNA Translation: AAA39348.1
CCDSiCCDS21189.1
PIRiA24378
RefSeqiNP_032483.1, NM_008457.3
UniGeneiMm.435488

Genome annotation databases

EnsembliENSMUST00000072204; ENSMUSP00000072063; ENSMUSG00000063089
GeneIDi16624
KEGGimmu:16624
UCSCiuc009gob.1 mouse

Similar proteinsi

Entry informationi

Entry nameiK1KB8_MOUSE
AccessioniPrimary (citable) accession number: P07628
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 23, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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