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Protein

Kallikrein 1-related peptidase b8

Gene

Klk1b8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei120 – 1201Charge relay system
Active sitei213 – 2131Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.118. 3474.
ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.
REACT_235886. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiS01.067.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b8 (EC:3.4.21.35)
Alternative name(s):
Glandular kallikrein K8
Short name:
mGK-8
Tissue kallikrein-8
Gene namesi
Name:Klk1b8
Synonyms:Klk-8, Klk8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:892018. Klk1b8.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818CuratedAdd
BLAST
Propeptidei19 – 246Activation peptideCuratedPRO_0000027977
Chaini25 – 261237Kallikrein 1-related peptidase b8PRO_0000027978Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 173PROSITE-ProRule annotation
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Glycosylationi102 – 1021N-linked (GlcNAc...)Curated
Disulfide bondi152 ↔ 219PROSITE-ProRule annotation
Disulfide bondi184 ↔ 198PROSITE-ProRule annotation
Disulfide bondi209 ↔ 234PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP07628.
PaxDbiP07628.
PRIDEiP07628.

Expressioni

Gene expression databases

BgeeiP07628.
CleanExiMM_KLK1B8.
MM_KLK8.
GenevestigatoriP07628.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072063.

Structurei

3D structure databases

ProteinModelPortaliP07628.
SMRiP07628. Positions 25-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 258234Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP07628.
KOiK01325.
OrthoDBiEOG75B84T.
PhylomeDBiP07628.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07628-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFLILFLAL SLGGIDAAPP LQSRVVGGFN CEKNSQPWQV AVYDNKEHIC
60 70 80 90 100
GGVLLERNWV LTAAHCYVDQ YEVWLGKNKL FQEEPSAQHR LVSKSFPHPG
110 120 130 140 150
FNMSLLTLKE IPPGADFSND LMLLRLSKPA DITDAVKPIT LPTKESKLGS
160 170 180 190 200
TCLASGWGSI TPTKWQKPDD LQCVFLKLLP IKNCIENHNV KVTDVMLCAG
210 220 230 240 250
EMSGGKNICK GDSGGPLICD SVLQGITSTG PIPCGKPGVP AMYTNLIKFN
260
SWIKDTMTKN S
Length:261
Mass (Da):28,531
Last modified:April 1, 1988 - v1
Checksum:iD0174F7093137322
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03994 mRNA. Translation: CAA27630.1.
M18587 Genomic DNA. Translation: AAA39347.1.
M18607 Genomic DNA. Translation: AAA39348.1.
CCDSiCCDS21189.1.
PIRiA24378.
RefSeqiNP_032483.1. NM_008457.2.
UniGeneiMm.435488.

Genome annotation databases

EnsembliENSMUST00000072204; ENSMUSP00000072063; ENSMUSG00000063089.
GeneIDi16624.
KEGGimmu:16624.
UCSCiuc009gob.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03994 mRNA. Translation: CAA27630.1.
M18587 Genomic DNA. Translation: AAA39347.1.
M18607 Genomic DNA. Translation: AAA39348.1.
CCDSiCCDS21189.1.
PIRiA24378.
RefSeqiNP_032483.1. NM_008457.2.
UniGeneiMm.435488.

3D structure databases

ProteinModelPortaliP07628.
SMRiP07628. Positions 25-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072063.

Protein family/group databases

MEROPSiS01.067.

Proteomic databases

MaxQBiP07628.
PaxDbiP07628.
PRIDEiP07628.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072204; ENSMUSP00000072063; ENSMUSG00000063089.
GeneIDi16624.
KEGGimmu:16624.
UCSCiuc009gob.1. mouse.

Organism-specific databases

CTDi16624.
MGIiMGI:892018. Klk1b8.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP07628.
KOiK01325.
OrthoDBiEOG75B84T.
PhylomeDBiP07628.
TreeFamiTF331065.

Enzyme and pathway databases

BRENDAi3.4.21.118. 3474.
ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.
REACT_235886. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

NextBioi290239.
PROiP07628.
SOURCEiSearch...

Gene expression databases

BgeeiP07628.
CleanExiMM_KLK1B8.
MM_KLK8.
GenevestigatoriP07628.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The sequence of a cDNA clone coding for a novel kallikrein from mouse submaxillary gland."
    Fahnestock M., Brundage S., Shooter E.M.
    Nucleic Acids Res. 14:4823-4835(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
    Evans B.A., Drinkwater C.C., Richards R.I.
    J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 16-54 AND 70-122.

Entry informationi

Entry nameiK1KB8_MOUSE
AccessioniPrimary (citable) accession number: P07628
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: February 4, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.