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P07628 (K1KB8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein 1-related peptidase b8

EC=3.4.21.35
Alternative name(s):
Glandular kallikrein K8
Short name=mGK-8
Tissue kallikrein-8
Gene names
Name:Klk1b8
Synonyms:Klk-8, Klk8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Probable
Propeptide19 – 246Activation peptide Probable
PRO_0000027977
Chain25 – 261237Kallikrein 1-related peptidase b8
PRO_0000027978

Regions

Domain25 – 258234Peptidase S1

Sites

Active site651Charge relay system
Active site1201Charge relay system
Active site2131Charge relay system

Amino acid modifications

Glycosylation1021N-linked (GlcNAc...) Probable
Disulfide bond31 ↔ 173 By similarity
Disulfide bond50 ↔ 66 By similarity
Disulfide bond152 ↔ 219 By similarity
Disulfide bond184 ↔ 198 By similarity
Disulfide bond209 ↔ 234 By similarity

Sequences

Sequence LengthMass (Da)Tools
P07628 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: D0174F7093137322

FASTA26128,531
        10         20         30         40         50         60 
MRFLILFLAL SLGGIDAAPP LQSRVVGGFN CEKNSQPWQV AVYDNKEHIC GGVLLERNWV 

        70         80         90        100        110        120 
LTAAHCYVDQ YEVWLGKNKL FQEEPSAQHR LVSKSFPHPG FNMSLLTLKE IPPGADFSND 

       130        140        150        160        170        180 
LMLLRLSKPA DITDAVKPIT LPTKESKLGS TCLASGWGSI TPTKWQKPDD LQCVFLKLLP 

       190        200        210        220        230        240 
IKNCIENHNV KVTDVMLCAG EMSGGKNICK GDSGGPLICD SVLQGITSTG PIPCGKPGVP 

       250        260 
AMYTNLIKFN SWIKDTMTKN S 

« Hide

References

[1]"The sequence of a cDNA clone coding for a novel kallikrein from mouse submaxillary gland."
Fahnestock M., Brundage S., Shooter E.M.
Nucleic Acids Res. 14:4823-4835(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
Evans B.A., Drinkwater C.C., Richards R.I.
J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 16-54 AND 70-122.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03994 mRNA. Translation: CAA27630.1.
M18587 Genomic DNA. Translation: AAA39347.1.
M18607 Genomic DNA. Translation: AAA39348.1.
CCDSCCDS21189.1.
PIRA24378.
RefSeqNP_032483.1. NM_008457.2.
UniGeneMm.435488.

3D structure databases

ProteinModelPortalP07628.
SMRP07628. Positions 25-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000072063.

Protein family/group databases

MEROPSS01.067.

Proteomic databases

MaxQBP07628.
PaxDbP07628.
PRIDEP07628.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000072204; ENSMUSP00000072063; ENSMUSG00000063089.
GeneID16624.
KEGGmmu:16624.
UCSCuc009gob.1. mouse.

Organism-specific databases

CTD16624.
MGIMGI:892018. Klk1b8.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00750000117405.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP07628.
KOK01325.
OMACKSEDHA.
OrthoDBEOG75B84T.
PhylomeDBP07628.
TreeFamTF331065.

Enzyme and pathway databases

BRENDA3.4.21.118. 3474.

Gene expression databases

BgeeP07628.
CleanExMM_KLK1B8.
MM_KLK8.
GenevestigatorP07628.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio290239.
PROP07628.
SOURCESearch...

Entry information

Entry nameK1KB8_MOUSE
AccessionPrimary (citable) accession number: P07628
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot