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Protein

Homoserine O-succinyltransferase

Gene

metA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Succinyl-CoA + L-homoserine = CoA + O-succinyl-L-homoserine.

Enzyme regulationi

Allosterically inhibited by SAM and methionine.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes O-succinyl-L-homoserine from L-homoserine.
Proteins known to be involved in this subpathway in this organism are:
  1. Homoserine O-succinyltransferase (metA)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes O-succinyl-L-homoserine from L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei142 – 1421Sequence analysis

GO - Molecular functioni

  • homoserine O-succinyltransferase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:HOMSUCTRAN-MONOMER.
ECOL316407:JW3973-MONOMER.
MetaCyc:HOMSUCTRAN-MONOMER.
UniPathwayiUPA00051; UER00075.

Names & Taxonomyi

Protein namesi
Recommended name:
Homoserine O-succinyltransferase (EC:2.3.1.46)
Alternative name(s):
Homoserine O-transsuccinylase
Short name:
HTS
Gene namesi
Name:metA
Ordered Locus Names:b4013, JW3973
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10581. metA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 309308Homoserine O-succinyltransferasePRO_0000199748Add
BLAST

Proteomic databases

EPDiP07623.
PaxDbiP07623.
PRIDEiP07623.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4259289. 12 interactions.
IntActiP07623. 2 interactions.
MINTiMINT-1343536.
STRINGi511145.b4013.

Structurei

3D structure databases

ProteinModelPortaliP07623.
SMRiP07623. Positions 2-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HTS family.Curated

Phylogenomic databases

eggNOGiENOG4105CVG. Bacteria.
COG1897. LUCA.
HOGENOMiHOG000115049.
InParanoidiP07623.
KOiK00651.
OMAiWRSHRNL.
OrthoDBiEOG61P6ST.
PhylomeDBiP07623.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00295. Homoser_O_succinyltr.
InterProiIPR029062. Class_I_gatase-like.
IPR005697. Homserine_suc_trans.
[Graphical view]
PANTHERiPTHR20919. PTHR20919. 1 hit.
PfamiPF04204. HTS. 1 hit.
[Graphical view]
PIRSFiPIRSF000450. H_ser_succinyltr. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01001. metA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIRVPDELP AVNFLREENV FVMTTSRASG QEIRPLKVLI LNLMPKKIET
60 70 80 90 100
ENQFLRLLSN SPLQVDIQLL RIDSRESRNT PAEHLNNFYC NFEDIQDQNF
110 120 130 140 150
DGLIVTGAPL GLVEFNDVAY WPQIKQVLEW SKDHVTSTLF VCWAVQAALN
160 170 180 190 200
ILYGIPKQTR TEKLSGVYEH HILHPHALLT RGFDDSFLAP HSRYADFPAA
210 220 230 240 250
LIRDYTDLEI LAETEEGDAY LFASKDKRIA FVTGHPEYDA QTLAQEFFRD
260 270 280 290 300
VEAGLDPDVP YNYFPHNDPQ NTPRASWRSH GNLLFTNWLN YYVYQITPYD

LRHMNPTLD
Length:309
Mass (Da):35,727
Last modified:January 23, 2007 - v4
Checksum:iA2C395A9A9CFE2C0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 671I → V (PubMed:2654885).Curated
Sequence conflicti67 – 671I → V (PubMed:6094503).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14501 Genomic DNA. Translation: CAA32654.1.
U00006 Genomic DNA. Translation: AAC43107.1.
U00096 Genomic DNA. Translation: AAC76983.1.
AP009048 Genomic DNA. Translation: BAE78015.1.
M10210 Genomic DNA. Translation: AAA24157.1.
X12431 Genomic DNA. No translation available.
PIRiD65208. XYECM.
RefSeqiNP_418437.1. NC_000913.3.
WP_001122779.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76983; AAC76983; b4013.
BAE78015; BAE78015; BAE78015.
GeneIDi948513.
KEGGiecj:JW3973.
eco:b4013.
PATRICi32123553. VBIEscCol129921_4125.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14501 Genomic DNA. Translation: CAA32654.1.
U00006 Genomic DNA. Translation: AAC43107.1.
U00096 Genomic DNA. Translation: AAC76983.1.
AP009048 Genomic DNA. Translation: BAE78015.1.
M10210 Genomic DNA. Translation: AAA24157.1.
X12431 Genomic DNA. No translation available.
PIRiD65208. XYECM.
RefSeqiNP_418437.1. NC_000913.3.
WP_001122779.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP07623.
SMRiP07623. Positions 2-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259289. 12 interactions.
IntActiP07623. 2 interactions.
MINTiMINT-1343536.
STRINGi511145.b4013.

Proteomic databases

EPDiP07623.
PaxDbiP07623.
PRIDEiP07623.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76983; AAC76983; b4013.
BAE78015; BAE78015; BAE78015.
GeneIDi948513.
KEGGiecj:JW3973.
eco:b4013.
PATRICi32123553. VBIEscCol129921_4125.

Organism-specific databases

EchoBASEiEB0576.
EcoGeneiEG10581. metA.

Phylogenomic databases

eggNOGiENOG4105CVG. Bacteria.
COG1897. LUCA.
HOGENOMiHOG000115049.
InParanoidiP07623.
KOiK00651.
OMAiWRSHRNL.
OrthoDBiEOG61P6ST.
PhylomeDBiP07623.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00075.
BioCyciEcoCyc:HOMSUCTRAN-MONOMER.
ECOL316407:JW3973-MONOMER.
MetaCyc:HOMSUCTRAN-MONOMER.

Miscellaneous databases

PROiP07623.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00295. Homoser_O_succinyltr.
InterProiIPR029062. Class_I_gatase-like.
IPR005697. Homserine_suc_trans.
[Graphical view]
PANTHERiPTHR20919. PTHR20919. 1 hit.
PfamiPF04204. HTS. 1 hit.
[Graphical view]
PIRSFiPIRSF000450. H_ser_succinyltr. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01001. metA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the metA gene encoding homoserine trans-succinylase in Escherichia coli."
    Cozzone A.J.
    Nucleic Acids Res. 17:2856-2856(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Escherichia coli metA-encoded homoserine transsuccinylase."
    Born T.L., Blanchard J.S.
    Biochemistry 38:14416-14423(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21, CHARACTERIZATION.
  3. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Regulatory region of the metA gene of Escherichia coli K-12."
    Michaeli S., Mevarech M., Ron E.Z.
    J. Bacteriol. 160:1158-1162(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-70.
    Strain: K12.
  7. "Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli."
    Byrne C.R., Stokes H.W., Ward K.A.
    Nucleic Acids Res. 16:9342-9342(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 207-309.
    Strain: K12.

Entry informationi

Entry nameiMETA_ECOLI
AccessioniPrimary (citable) accession number: P07623
Secondary accession number(s): Q2M6U1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 138 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.