P07623 (META_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine O-succinyltransferase
EC=2.3.1.46

Alternative name(s):
Homoserine O-transsuccinylase
Short name=HTS

Gene names

Name:	metA
Ordered Locus Names:	b4013, JW3973

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Succinyl-CoA + L-homoserine = CoA + O-succinyl-L-homoserine. HAMAP-Rule MF_00295
Enzyme regulation	Allosterically inhibited by SAM and methionine. HAMAP-Rule MF_00295
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-succinyl-L-homoserine from L-homoserine: step 1/1. HAMAP-Rule MF_00295
Subunit structure	Homodimer.
Subcellular location	Cytoplasm HAMAP-Rule MF_00295.
Sequence similarities	Belongs to the HTS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Allosteric enzyme Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	L-methionine biosynthetic process from homoserine via O-succinyl-L-homoserine and cystathionine Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	homoserine O-succinyltransferase activity Inferred from direct assay Ref.2. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 309	308	Homoserine O-succinyltransferase HAMAP-Rule MF_00295		PRO_0000199748
Sites
	Active site	142	1	Potential
Experimental info
	Sequence conflict	67	1	I → V Ref.1
	Sequence conflict	67	1	I → V Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P07623 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: A2C395A9A9CFE2C0	FASTA	309	35,727
	10 20 30 40 50 60 MPIRVPDELP AVNFLREENV FVMTTSRASG QEIRPLKVLI LNLMPKKIET ENQFLRLLSN 70 80 90 100 110 120 SPLQVDIQLL RIDSRESRNT PAEHLNNFYC NFEDIQDQNF DGLIVTGAPL GLVEFNDVAY 130 140 150 160 170 180 WPQIKQVLEW SKDHVTSTLF VCWAVQAALN ILYGIPKQTR TEKLSGVYEH HILHPHALLT 190 200 210 220 230 240 RGFDDSFLAP HSRYADFPAA LIRDYTDLEI LAETEEGDAY LFASKDKRIA FVTGHPEYDA 250 260 270 280 290 300 QTLAQEFFRD VEAGLDPDVP YNYFPHNDPQ NTPRASWRSH GNLLFTNWLN YYVYQITPYD LRHMNPTLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the metA gene encoding homoserine trans-succinylase in Escherichia coli." Cozzone A.J. Nucleic Acids Res. 17:2856-2856(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Escherichia coli metA-encoded homoserine transsuccinylase." Born T.L., Blanchard J.S. Biochemistry 38:14416-14423(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21, CHARACTERIZATION.
[3]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Regulatory region of the metA gene of Escherichia coli K-12." Michaeli S., Mevarech M., Ron E.Z. J. Bacteriol. 160:1158-1162(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-70. Strain: K12.
[7]	"Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli." Byrne C.R., Stokes H.W., Ward K.A. Nucleic Acids Res. 16:9342-9342(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 207-309. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14501 Genomic DNA. Translation: CAA32654.1. U00006 Genomic DNA. Translation: AAC43107.1. U00096 Genomic DNA. Translation: AAC76983.1. AP009048 Genomic DNA. Translation: BAE78015.1. M10210 Genomic DNA. Translation: AAA24157.1. X12431 Genomic DNA. No translation available.
PIR	XYECM. D65208.
RefSeq	NP_418437.1. NC_000913.2. YP_492156.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P07623.
SMR	P07623. Positions 2-297.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07623. 1 interaction.
MINT	MINT-1343536.
STRING	511145.b4013.
Proteomic databases
PaxDb	P07623.
PRIDE	P07623.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76983; AAC76983; b4013. BAE78015; BAE78015; BAE78015.
GeneID	12930295. 948513.
KEGG	ecj:Y75_p3900. eco:b4013.
PATRIC	32123553. VBIEscCol129921_4125.
Organism-specific databases
EchoBASE	EB0576.
EcoGene	EG10581. metA.
Phylogenomic databases
eggNOG	COG1897.
HOGENOM	HOG000115049.
KO	K00651.
OMA	WRSHRNL.
ProtClustDB	PRK05368.
Enzyme and pathway databases
BioCyc	EcoCyc:HOMSUCTRAN-MONOMER. ECOL316407:JW3973-MONOMER. MetaCyc:HOMSUCTRAN-MONOMER.
UniPathway	UPA00051; UER00075.
Gene expression databases
Genevestigator	P07623.
Family and domain databases
HAMAP	MF_00295. Homoser_O-succinyltr.
InterPro	IPR005697. Homserine_suc_trans. [Graphical view]
PANTHER	PTHR20919. PTHR20919. 1 hit.
Pfam	PF04204. HTS. 1 hit. [Graphical view]
PIRSF	PIRSF000450. H_ser_succinyltr. 1 hit.
ProDom	PD037892. Homserine_suc_trans. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01001. metA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

META_ECOLI

Accession

Primary (citable) accession number: P07623
Secondary accession number(s): Q2M6U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 118 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program