P07618 (J5_VACCW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein J5

Alternative name(s):
Protein F11

Gene names

Ordered Locus Names:	VACWR097
ORF Names:	J5L

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Envelope protein part of the entry-fusion complex responsible for the virus membrane fusion with host cell membrane during virus entry. Also plays a role in cell-cell fusion (syncytium formation).
Subunit structure	Part of a stable entry-fusion complex (EFC) which is at least composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. Formation of the viral membrane is necessary for the assembly of the complex.
Subcellular location	Virion membrane; Single-pass membrane protein Potential. Note: Component of the mature virion (MV) membrane. The mature virion is located in the cytoplasm of infected cells and is probably released by cell lysis.
Sequence similarities	Belongs to the poxviridae A16/G9/J5 family.
Sequence caution	The sequence CAA26019.1 differs from that shown. Reason: Frameshift at position 124.

Ontologies

Keywords
Biological process	Fusion of virus membrane with host cell membrane Fusion of virus membrane with host membrane Initiation of viral infection Viral penetration into host cytoplasm
Cellular component	Membrane Viral envelope protein Virion
Domain	Signal-anchor Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 133	133	Protein J5		PRO_0000099595
Regions
	Topological domain	1 – 111	111	Cytoplasmic Probable
	Transmembrane	112 – 132	21	Helical; Signal-anchor; Potential

Sequences

	Sequence		Length	Mass (Da)
	P07618 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: FE7D976F31B87309	FASTA	133	15,158
	10 20 30 40 50 60 MTDEQIYAFC DANKDDIRCK CIYPDKSIVR IGIDTRLPYY CWYEPCKRSD ALLPASLKKN 70 80 90 100 110 120 ITKCNVSDCT ISLGNVSITD SKLDVNNVCD SKRVATENIA VRYLNQEIRY PIIDIKWLPI 130 GLLALAILIL AFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a cluster of early and late genes in a conserved segment of the vaccinia virus genome." Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R.E. Nucleic Acids Res. 13:985-998(1985) [PubMed: 2987815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Homology between RNA polymerases of poxviruses, prokaryotes, and eukaryotes: nucleotide sequence and transcriptional analysis of vaccinia virus genes encoding 147-kDa and 22-kDa subunits." Broyles S.S., Moss B. Proc. Natl. Acad. Sci. U.S.A. 83:3141-3145(1986) [PubMed: 3517852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb." Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Poxvirus multiprotein entry-fusion complex." Senkevich T.G., Ojeda S., Townsley A., Nelson G.E., Moss B. Proc. Natl. Acad. Sci. U.S.A. 102:18572-18577(2005) [PubMed: 16339313] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH A16; A21; A28; G3; G9; H2 AND L5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X01978 Genomic DNA. Translation: CAA26019.1. Frameshift. M13209 Genomic DNA. Translation: AAB59834.1. AY243312 Genomic DNA. Translation: AAO89376.1.
PIR	QQVZEL. B26216.
RefSeq	YP_232979.1. NC_006998.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-6740551.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3707553.
Phylogenomic databases
ProtClustDB	CLSP2509783.
Family and domain databases
InterPro	IPR004251. Vaccinia_virus_A16. [Graphical view]
Pfam	PF03003. DUF230. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

J5_VACCW

Accession

Primary (citable) accession number: P07618

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	December 7, 2004
Last modified:	October 19, 2011
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program