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Protein

Protein L1

Gene

VACWR088

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Envelope protein which probably plays a role in virus entry into the host cell. Is probably involved in the virus attachment to the host cell surface and associates with the entry/fusion complex (EFC). Needed for fusion and penetration of the virus core into host cell.3 Publications

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Protein family/group databases

TCDBi1.G.11.1.1. poxvirus cell entry protein complex (pep-c) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein L1
Alternative name(s):
Virion membrane protein M25
Gene namesi
Ordered Locus Names:VACWR088
ORF Names:L1R
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000000344 Componenti: Genome

Subcellular locationi

  • Virion membrane Curated; Single-pass membrane protein Curated

  • Note: Localizes to the membrane surrounding the core of mature virus particles (MV).1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 183Virion surfaceSequence analysisAdd BLAST182
Transmembranei184 – 204HelicalSequence analysisAdd BLAST21
Topological domaini205 – 250IntravirionSequence analysisAdd BLAST46

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Viral envelope protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Complete loss of myristoylation. Blocks the maturation of virus. 2 Publications1
Mutagenesisi34C → S: 75% loss in virus production. 1 Publication1
Mutagenesisi49C → S: 75% loss in virus production. 1 Publication1
Mutagenesisi57C → S: Almost no effect on virus production. 1 Publication1
Mutagenesisi116C → S: 50% loss in virus production. 1 Publication1
Mutagenesisi136C → S: 70% loss in virus production. 1 Publication1
Mutagenesisi158C → S: 60% loss in virus production. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by host
ChainiPRO_00000996132 – 250Protein L1Add BLAST249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by host2 Publications1
Disulfide bondi34 ↔ 571 Publication
Disulfide bondi49 ↔ 1361 Publication
Disulfide bondi116 ↔ 1581 Publication

Post-translational modificationi

Myristoylated.2 Publications

Keywords - PTMi

Disulfide bond, Lipoprotein, Myristate

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.1 Publication

Interactioni

Subunit structurei

Interacts with G4; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer.1 Publication

Structurei

Secondary structure

1250
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 25Combined sources21
Beta strandi35 – 55Combined sources21
Helixi61 – 77Combined sources17
Helixi81 – 84Combined sources4
Helixi87 – 95Combined sources9
Turni101 – 103Combined sources3
Helixi104 – 115Combined sources12
Beta strandi116 – 118Combined sources3
Helixi119 – 122Combined sources4
Beta strandi127 – 137Combined sources11
Beta strandi140 – 142Combined sources3
Beta strandi144 – 150Combined sources7
Helixi154 – 174Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YPYX-ray1.51A/B2-185[»]
2I9LX-ray3.10I/J/K/L2-185[»]
4U6HX-ray3.10E/J1-184[»]
ProteinModelPortaliP07612.
SMRiP07612.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07612.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 12Targeting to MV membraneAdd BLAST11

Sequence similaritiesi

Belongs to the chordopoxvirinae L1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090000VH.

Family and domain databases

InterProiView protein in InterPro
IPR003472. Virion_mem_poxvirus_L1.
PfamiView protein in Pfam
PF02442. L1R_F9L. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAAASIQTT VNTLSERISS KLEQEANASA QTKCDIEIGN FYIRQNHGCN
60 70 80 90 100
LTVKNMCSAD ADAQLDAVLS AATETYSGLT PEQKAYVPAM FTAALNIQTS
110 120 130 140 150
VNTVVRDFEN YVKQTCNSSA VVDNKLKIQN VIIDECYGAP GSPTNLEFIN
160 170 180 190 200
TGSSKGNCAI KALMQLTTKA TTQIAPKQVA GTGVQFYMIV IGVIILAALF
210 220 230 240 250
MYYAKRMLFT STNDKIKLIL ANKENVHWTT YMDTFFRTSP MVIATTDMQN
Length:250
Mass (Da):27,279
Last modified:January 23, 2007 - v3
Checksum:i28FE89850863372D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01978 Genomic DNA. Translation: CAA26010.1.
AY243312 Genomic DNA. Translation: AAO89367.1.
PIRiB23092. QQVZF2.
RefSeqiYP_232970.1. NC_006998.1.

Genome annotation databases

GeneIDi3707544.
KEGGivg:3707544.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiL1_VACCW
AccessioniPrimary (citable) accession number: P07612
Secondary accession number(s): Q76ZT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: March 15, 2017
This is version 91 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families