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Protein

Thymidylate synthase

Gene

Tyms

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44dUMPBy similarity1
Active sitei189NucleophileBy similarity1
Binding sitei2125,10-methylenetetrahydrofolateBy similarity1
Binding sitei220dUMPBy similarity1
Binding sitei3065,10-methylenetetrahydrofolate; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi169 – 170dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi209 – 212dUMPBy similarity4
Nucleotide bindingi250 – 252dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-499943. Interconversion of nucleotide di- and triphosphates.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:Tyms
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:98878. Tyms.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3160.
GuidetoPHARMACOLOGYi2642.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409021 – 307Thymidylate synthaseAdd BLAST307

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei108PhosphoserineBy similarity1
Cross-linki286Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki302Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP07607.
PaxDbiP07607.
PeptideAtlasiP07607.
PRIDEiP07607.

2D gel databases

REPRODUCTION-2DPAGEiP07607.

PTM databases

iPTMnetiP07607.
PhosphoSitePlusiP07607.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025747.
CleanExiMM_TYMS.
ExpressionAtlasiP07607. baseline and differential.
GenevisibleiP07607. MM.

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026846.

Chemistry databases

BindingDBiP07607.

Structurei

Secondary structure

1307
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 37Combined sources14
Beta strandi39 – 41Combined sources3
Beta strandi44 – 47Combined sources4
Beta strandi49 – 60Combined sources12
Beta strandi62 – 64Combined sources3
Beta strandi69 – 71Combined sources3
Helixi75 – 86Combined sources12
Helixi92 – 96Combined sources5
Turni97 – 99Combined sources3
Turni102 – 107Combined sources6
Helixi109 – 114Combined sources6
Helixi129 – 135Combined sources7
Helixi154 – 164Combined sources11
Beta strandi172 – 174Combined sources3
Turni178 – 180Combined sources3
Helixi181 – 183Combined sources3
Beta strandi184 – 186Combined sources3
Beta strandi189 – 198Combined sources10
Beta strandi201 – 212Combined sources12
Turni213 – 215Combined sources3
Helixi216 – 235Combined sources20
Beta strandi238 – 252Combined sources15
Helixi253 – 255Combined sources3
Helixi256 – 263Combined sources8
Beta strandi272 – 275Combined sources4
Helixi282 – 284Combined sources3
Helixi287 – 289Combined sources3
Beta strandi290 – 294Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IHIX-ray1.94A/B1-307[»]
4E5OX-ray1.70A/B/C/D/E/F1-307[»]
4EB4X-ray1.74A/B/C/D1-307[»]
4EINX-ray1.75A/B1-307[»]
4EZ8X-ray1.17A1-307[»]
5FCTX-ray1.55A/B1-307[»]
ProteinModelPortaliP07607.
SMRiP07607.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07607.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiKOG0673. Eukaryota.
COG0207. LUCA.
GeneTreeiENSGT00390000014786.
HOGENOMiHOG000257899.
HOVERGENiHBG001934.
InParanoidiP07607.
KOiK00560.
OMAiKQYLDLC.
OrthoDBiEOG091G0CV0.
PhylomeDBiP07607.
TreeFamiTF353027.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiView protein in InterPro
IPR023451. Thymidate_synth/dCMP_Mease.
IPR036926. Thymidate_synth/dCMP_Mease_sf.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
PfamiView protein in Pfam
PF00303. Thymidylat_synt. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiView protein in PROSITE
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P07607-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVVGSELQS DAQQLSAEAP RHGELQYLRQ VEHILRCGFK KEDRTGTGTL
60 70 80 90 100
SVFGMQARYS LRDEFPLLTT KRVFWKGVLE ELLWFIKGST NAKELSSKGV
110 120 130 140 150
RIWDANGSRD FLDSLGFSAR QEGDLGPVYG FQWRHFGAEY KDMDSDYSGQ
160 170 180 190 200
GVDQLQKVID TIKTNPDDRR IIMCAWNPKD LPLMALPPCH ALCQFYVVNG
210 220 230 240 250
ELSCQLYQRS GDMGLGVPFN IASYALLTYM IAHITGLQPG DFVHTLGDAH
260 270 280 290 300
IYLNHIEPLK IQLQREPRPF PKLKILRKVE TIDDFKVEDF QIEGYNPHPT

IKMEMAV
Length:307
Mass (Da):34,958
Last modified:April 1, 1988 - v1
Checksum:iE4930618C487FD5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13019 mRNA. Translation: AAA40439.1.
M13352
, J02617, M13347, M13348, M13349, M13350, M13351 Genomic DNA. Translation: AAA40444.1.
X14489 mRNA. Translation: CAA32651.1.
CCDSiCCDS19154.1.
PIRiA26323. YXMST.
RefSeqiNP_067263.1. NM_021288.4.
UniGeneiMm.268395.

Genome annotation databases

EnsembliENSMUST00000026846; ENSMUSP00000026846; ENSMUSG00000025747.
GeneIDi22171.
KEGGimmu:22171.
UCSCiuc008wux.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiTYSY_MOUSE
AccessioniPrimary (citable) accession number: P07607
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: October 25, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families