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P07607

- TYSY_MOUSE

UniProt

P07607 - TYSY_MOUSE

Protein

Thymidylate synthase

Gene

Tyms

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.By similarity

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei189 – 1891By similarity

    GO - Molecular functioni

    1. cofactor binding Source: Ensembl
    2. drug binding Source: Ensembl
    3. folic acid binding Source: Ensembl
    4. mRNA binding Source: Ensembl
    5. nucleotide binding Source: Ensembl
    6. thymidylate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. aging Source: Ensembl
    2. cartilage development Source: Ensembl
    3. circadian rhythm Source: Ensembl
    4. developmental growth Source: Ensembl
    5. dTMP biosynthetic process Source: Ensembl
    6. dTTP biosynthetic process Source: UniProtKB-UniPathway
    7. dUMP metabolic process Source: Ensembl
    8. immortalization of host cell by virus Source: Ensembl
    9. intestinal epithelial cell maturation Source: Ensembl
    10. organ regeneration Source: Ensembl
    11. polysaccharide metabolic process Source: Ensembl
    12. response to cytokine Source: Ensembl
    13. response to drug Source: Ensembl
    14. response to ethanol Source: Ensembl
    15. response to folic acid Source: Ensembl
    16. response to glucocorticoid Source: Ensembl
    17. response to organophosphorus Source: Ensembl
    18. response to progesterone Source: Ensembl
    19. response to toxic substance Source: Ensembl
    20. response to vitamin A Source: Ensembl
    21. tetrahydrofolate metabolic process Source: Ensembl
    22. uracil metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    ReactomeiREACT_198608. G1/S-Specific Transcription.
    REACT_206830. E2F mediated regulation of DNA replication.
    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthase (EC:2.1.1.45)
    Short name:
    TS
    Short name:
    TSase
    Gene namesi
    Name:Tyms
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:98878. Tyms.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity. Mitochondrion By similarity. Mitochondrion matrix By similarity. Mitochondrion inner membrane By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. mitochondrial inner membrane Source: UniProtKB
    3. mitochondrial matrix Source: UniProtKB
    4. mitochondrion Source: UniProtKB
    5. nucleolus Source: Ensembl
    6. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 307306Thymidylate synthasePRO_0000140902Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei108 – 1081PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP07607.
    PRIDEiP07607.

    2D gel databases

    REPRODUCTION-2DPAGEP07607.

    PTM databases

    PhosphoSiteiP07607.

    Expressioni

    Gene expression databases

    ArrayExpressiP07607.
    BgeeiP07607.
    CleanExiMM_TYMS.
    GenevestigatoriP07607.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    Secondary structure

    1
    307
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi24 – 3714
    Beta strandi39 – 413
    Beta strandi44 – 474
    Beta strandi49 – 6012
    Beta strandi62 – 643
    Beta strandi69 – 713
    Helixi75 – 8612
    Helixi92 – 965
    Turni97 – 993
    Turni102 – 1076
    Helixi109 – 1146
    Helixi129 – 1357
    Helixi154 – 16411
    Beta strandi172 – 1743
    Turni178 – 1803
    Helixi181 – 1833
    Beta strandi184 – 1863
    Beta strandi189 – 19810
    Beta strandi201 – 21212
    Turni213 – 2153
    Helixi216 – 23520
    Beta strandi238 – 25215
    Helixi253 – 2553
    Helixi256 – 2638
    Beta strandi272 – 2754
    Helixi282 – 2843
    Helixi287 – 2893
    Beta strandi290 – 2945

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3IHIX-ray1.94A/B1-307[»]
    4E5OX-ray1.70A/B/C/D/E/F1-307[»]
    4EB4X-ray1.74A/B/C/D1-307[»]
    4EINX-ray1.75A/B1-307[»]
    4EZ8X-ray1.17A1-307[»]
    ProteinModelPortaliP07607.
    SMRiP07607. Positions 21-307.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07607.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG0207.
    HOGENOMiHOG000257899.
    HOVERGENiHBG001934.
    InParanoidiP07607.
    KOiK00560.
    OMAiDVHLYSN.
    PhylomeDBiP07607.
    TreeFamiTF353027.

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 1 hit.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07607-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLVVGSELQS DAQQLSAEAP RHGELQYLRQ VEHILRCGFK KEDRTGTGTL    50
    SVFGMQARYS LRDEFPLLTT KRVFWKGVLE ELLWFIKGST NAKELSSKGV 100
    RIWDANGSRD FLDSLGFSAR QEGDLGPVYG FQWRHFGAEY KDMDSDYSGQ 150
    GVDQLQKVID TIKTNPDDRR IIMCAWNPKD LPLMALPPCH ALCQFYVVNG 200
    ELSCQLYQRS GDMGLGVPFN IASYALLTYM IAHITGLQPG DFVHTLGDAH 250
    IYLNHIEPLK IQLQREPRPF PKLKILRKVE TIDDFKVEDF QIEGYNPHPT 300
    IKMEMAV 307
    Length:307
    Mass (Da):34,958
    Last modified:April 1, 1988 - v1
    Checksum:iE4930618C487FD5E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13019 mRNA. Translation: AAA40439.1.
    M13352
    , J02617, M13347, M13348, M13349, M13350, M13351 Genomic DNA. Translation: AAA40444.1.
    X14489 mRNA. Translation: CAA32651.1.
    CCDSiCCDS19154.1.
    PIRiA26323. YXMST.
    RefSeqiNP_067263.1. NM_021288.4.
    UniGeneiMm.268395.

    Genome annotation databases

    EnsembliENSMUST00000026846; ENSMUSP00000026846; ENSMUSG00000025747.
    GeneIDi22171.
    KEGGimmu:22171.
    UCSCiuc008wux.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13019 mRNA. Translation: AAA40439.1 .
    M13352
    , J02617 , M13347 , M13348 , M13349 , M13350 , M13351 Genomic DNA. Translation: AAA40444.1 .
    X14489 mRNA. Translation: CAA32651.1 .
    CCDSi CCDS19154.1.
    PIRi A26323. YXMST.
    RefSeqi NP_067263.1. NM_021288.4.
    UniGenei Mm.268395.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3IHI X-ray 1.94 A/B 1-307 [» ]
    4E5O X-ray 1.70 A/B/C/D/E/F 1-307 [» ]
    4EB4 X-ray 1.74 A/B/C/D 1-307 [» ]
    4EIN X-ray 1.75 A/B 1-307 [» ]
    4EZ8 X-ray 1.17 A 1-307 [» ]
    ProteinModelPortali P07607.
    SMRi P07607. Positions 21-307.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P07607.
    ChEMBLi CHEMBL3160.
    GuidetoPHARMACOLOGYi 2642.

    PTM databases

    PhosphoSitei P07607.

    2D gel databases

    REPRODUCTION-2DPAGE P07607.

    Proteomic databases

    PaxDbi P07607.
    PRIDEi P07607.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026846 ; ENSMUSP00000026846 ; ENSMUSG00000025747 .
    GeneIDi 22171.
    KEGGi mmu:22171.
    UCSCi uc008wux.2. mouse.

    Organism-specific databases

    CTDi 7298.
    MGIi MGI:98878. Tyms.

    Phylogenomic databases

    eggNOGi COG0207.
    HOGENOMi HOG000257899.
    HOVERGENi HBG001934.
    InParanoidi P07607.
    KOi K00560.
    OMAi DVHLYSN.
    PhylomeDBi P07607.
    TreeFami TF353027.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .
    Reactomei REACT_198608. G1/S-Specific Transcription.
    REACT_206830. E2F mediated regulation of DNA replication.

    Miscellaneous databases

    EvolutionaryTracei P07607.
    NextBioi 302116.
    PROi P07607.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P07607.
    Bgeei P07607.
    CleanExi MM_TYMS.
    Genevestigatori P07607.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 1 hit.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of a cDNA for mouse thymidylate synthase reveals striking similarity with the prokaryotic enzyme."
      Perryman S.M., Rossana C., Deng T., Vanin E.F., Johnson L.F.
      Mol. Biol. Evol. 3:313-321(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structure of the gene for mouse thymidylate synthase. Locations of introns and multiple transcriptional start sites."
      Deng T., Li D., Jenh C.-H., Johnson L.F.
      J. Biol. Chem. 261:16000-16005(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Thymidylate synthase gene expression is stimulated by some (but not all) introns."
      Deng T., Li Y., Johnson L.F.
      Nucleic Acids Res. 17:645-658(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 236-265.

    Entry informationi

    Entry nameiTYSY_MOUSE
    AccessioniPrimary (citable) accession number: P07607
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3