Thymidylate synthase - Mus musculus (Mouse)

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P07607 (TYSY_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 106. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:

Tyms

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Contributes to the de novo mitochondrial thymidylate biosynthesis pathway By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
Pathway	Pyrimidine metabolism; dTTP biosynthesis.
Subunit structure	Homodimer.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Mitochondrion By similarity. Mitochondrion matrix By similarity. Mitochondrion inner membrane By similarity.
Sequence similarities	Belongs to the thymidylate synthase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm Membrane Mitochondrion Mitochondrion inner membrane Nucleus
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from electronic annotation. Source: Compara cartilage development Inferred from electronic annotation. Source: Compara circadian rhythm Inferred from electronic annotation. Source: Compara dTMP biosynthetic process Inferred from electronic annotation. Source: Compara dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUMP metabolic process Inferred from electronic annotation. Source: Compara developmental growth Inferred from electronic annotation. Source: Compara immortalization of host cell by virus Inferred from electronic annotation. Source: Compara intestinal epithelial cell maturation Inferred from electronic annotation. Source: Compara organ regeneration Inferred from electronic annotation. Source: Compara polysaccharide metabolic process Inferred from electronic annotation. Source: Compara response to cytokine stimulus Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to folic acid Inferred from electronic annotation. Source: Compara response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara response to organophosphorus Inferred from electronic annotation. Source: Compara response to progesterone stimulus Inferred from electronic annotation. Source: Compara response to toxin Inferred from electronic annotation. Source: Compara response to vitamin A Inferred from electronic annotation. Source: Compara tetrahydrofolate metabolic process Inferred from electronic annotation. Source: Compara uracil metabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrial inner membrane Inferred from sequence or structural similarity. Source: UniProtKB mitochondrial matrix Inferred from sequence or structural similarity. Source: UniProtKB nucleolus Inferred from electronic annotation. Source: Compara nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cofactor binding Inferred from electronic annotation. Source: Compara drug binding Inferred from electronic annotation. Source: Compara folic acid binding Inferred from electronic annotation. Source: Compara mRNA binding Inferred from electronic annotation. Source: Compara nucleotide binding Inferred from electronic annotation. Source: Compara thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 307

306

Thymidylate synthase

PRO_0000140902

Sites

Active site

189

By similarity

Amino acid modifications

Modified residue

108

Phosphoserine By similarity

Secondary structure

307

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P07607 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: E4930618C487FD5E
Show »

FASTA

307

34,958

        10         20         30         40         50         60 
MLVVGSELQS DAQQLSAEAP RHGELQYLRQ VEHILRCGFK KEDRTGTGTL SVFGMQARYS 

        70         80         90        100        110        120 
LRDEFPLLTT KRVFWKGVLE ELLWFIKGST NAKELSSKGV RIWDANGSRD FLDSLGFSAR 

       130        140        150        160        170        180 
QEGDLGPVYG FQWRHFGAEY KDMDSDYSGQ GVDQLQKVID TIKTNPDDRR IIMCAWNPKD 

       190        200        210        220        230        240 
LPLMALPPCH ALCQFYVVNG ELSCQLYQRS GDMGLGVPFN IASYALLTYM IAHITGLQPG 

       250        260        270        280        290        300 
DFVHTLGDAH IYLNHIEPLK IQLQREPRPF PKLKILRKVE TIDDFKVEDF QIEGYNPHPT 


IKMEMAV

« Hide

References

[1]	"Sequence of a cDNA for mouse thymidylate synthase reveals striking similarity with the prokaryotic enzyme." Perryman S.M., Rossana C., Deng T., Vanin E.F., Johnson L.F. Mol. Biol. Evol. 3:313-321(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure of the gene for mouse thymidylate synthase. Locations of introns and multiple transcriptional start sites." Deng T., Li D., Jenh C.-H., Johnson L.F. J. Biol. Chem. 261:16000-16005(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Thymidylate synthase gene expression is stimulated by some (but not all) introns." Deng T., Li Y., Johnson L.F. Nucleic Acids Res. 17:645-658(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 236-265.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M13019 mRNA. Translation: AAA40439.1.
M13352

M13351 Genomic DNA. Translation: AAA40444.1.
X14489 mRNA. Translation: CAA32651.1.

IPI

IPI00130734.

PIR

YXMST. A26323.

RefSeq

NP_067263.1. NM_021288.4.

UniGene

Mm.268395.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IHI	X-ray	1.94	A/B	1-307	[»]
4E5O	X-ray	1.70	A/B/C/D/E/F	1-307	[»]
4EB4	X-ray	1.74	A/B/C/D	1-307	[»]

ProteinModelPortal

P07607.

SMR

P07607. Positions 20-307.

ModBase

Search...

PTM databases

PhosphoSite

P07607.

2D gel databases

REPRODUCTION-2DPAGE

P07607.

Proteomic databases

PaxDb

P07607.

PRIDE

P07607.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000026846; ENSMUSP00000026846; ENSMUSG00000025747.

GeneID

22171.

KEGG

mmu:22171.

Organism-specific databases

CTD

7298.

MGI

MGI:98878. Tyms.

Phylogenomic databases

eggNOG

COG0207.

HOGENOM

HOG000257899.

HOVERGEN

HBG001934.

InParanoid

P07607.

K00560.

OMA

DTNVAYL.

OrthoDB

EOG4ZKJMP.

Enzyme and pathway databases

UniPathway

UPA00575.

Gene expression databases

ArrayExpress

P07607.

Bgee

P07607.

CleanEx

MM_TYMS.

Genevestigator

P07607.

GermOnline

ENSMUSG00000025747. Mus musculus.

Family and domain databases

Gene3D

3.30.572.10. 1 hit.

InterPro

IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]

Pfam

PF00303. Thymidylat_synt. 1 hit.
[Graphical view]

PRINTS

PR00108. THYMDSNTHASE.

SUPFAM

SSF55831. Thymidylat_synth_C. 1 hit.

TIGRFAMs

TIGR03284. thym_sym. 1 hit.

PROSITE

PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P07607.

ChEMBL

CHEMBL3160.

EvolutionaryTrace

P07607.

NextBio

302116.

SOURCE

Search...

Entry information

Entry name

TYSY_MOUSE

Accession

Primary (citable) accession number: P07607

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	April 3, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents