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P07607

- TYSY_MOUSE

UniProt

P07607 - TYSY_MOUSE

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Protein
Thymidylate synthase
Gene
Tyms
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Contributes to the de novo mitochondrial thymidylate biosynthesis pathway By similarity.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei189 – 1891 By similarity

GO - Molecular functioni

  1. cofactor binding Source: Ensembl
  2. drug binding Source: Ensembl
  3. folic acid binding Source: Ensembl
  4. mRNA binding Source: Ensembl
  5. nucleotide binding Source: Ensembl
  6. thymidylate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. aging Source: Ensembl
  2. cartilage development Source: Ensembl
  3. circadian rhythm Source: Ensembl
  4. dTMP biosynthetic process Source: Ensembl
  5. dTTP biosynthetic process Source: UniProtKB-UniPathway
  6. dUMP metabolic process Source: Ensembl
  7. developmental growth Source: Ensembl
  8. immortalization of host cell by virus Source: Ensembl
  9. intestinal epithelial cell maturation Source: Ensembl
  10. organ regeneration Source: Ensembl
  11. polysaccharide metabolic process Source: Ensembl
  12. response to cytokine Source: Ensembl
  13. response to drug Source: Ensembl
  14. response to ethanol Source: Ensembl
  15. response to folic acid Source: Ensembl
  16. response to glucocorticoid Source: Ensembl
  17. response to organophosphorus Source: Ensembl
  18. response to progesterone Source: Ensembl
  19. response to toxic substance Source: Ensembl
  20. response to vitamin A Source: Ensembl
  21. tetrahydrofolate metabolic process Source: Ensembl
  22. uracil metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

ReactomeiREACT_198608. G1/S-Specific Transcription.
REACT_206830. E2F mediated regulation of DNA replication.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:Tyms
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:98878. Tyms.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity. Mitochondrion By similarity. Mitochondrion matrix By similarity. Mitochondrion inner membrane By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. mitochondrial inner membrane Source: UniProtKB
  3. mitochondrial matrix Source: UniProtKB
  4. mitochondrion Source: UniProtKB
  5. nucleolus Source: Ensembl
  6. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 307306Thymidylate synthaseUniRule annotation
PRO_0000140902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei108 – 1081Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP07607.
PRIDEiP07607.

2D gel databases

REPRODUCTION-2DPAGEP07607.

PTM databases

PhosphoSiteiP07607.

Expressioni

Gene expression databases

ArrayExpressiP07607.
BgeeiP07607.
CleanExiMM_TYMS.
GenevestigatoriP07607.

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi24 – 3714
Beta strandi39 – 413
Beta strandi44 – 474
Beta strandi49 – 6012
Beta strandi62 – 643
Beta strandi69 – 713
Helixi75 – 8612
Helixi92 – 965
Turni97 – 993
Turni102 – 1076
Helixi109 – 1146
Helixi129 – 1357
Helixi154 – 16411
Beta strandi172 – 1743
Turni178 – 1803
Helixi181 – 1833
Beta strandi184 – 1863
Beta strandi189 – 19810
Beta strandi201 – 21212
Turni213 – 2153
Helixi216 – 23520
Beta strandi238 – 25215
Helixi253 – 2553
Helixi256 – 2638
Beta strandi272 – 2754
Helixi282 – 2843
Helixi287 – 2893
Beta strandi290 – 2945

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IHIX-ray1.94A/B1-307[»]
4E5OX-ray1.70A/B/C/D/E/F1-307[»]
4EB4X-ray1.74A/B/C/D1-307[»]
4EINX-ray1.75A/B1-307[»]
4EZ8X-ray1.17A1-307[»]
ProteinModelPortaliP07607.
SMRiP07607. Positions 21-307.

Miscellaneous databases

EvolutionaryTraceiP07607.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257899.
HOVERGENiHBG001934.
InParanoidiP07607.
KOiK00560.
OMAiDVHLYSN.
PhylomeDBiP07607.
TreeFamiTF353027.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07607-1 [UniParc]FASTAAdd to Basket

« Hide

MLVVGSELQS DAQQLSAEAP RHGELQYLRQ VEHILRCGFK KEDRTGTGTL    50
SVFGMQARYS LRDEFPLLTT KRVFWKGVLE ELLWFIKGST NAKELSSKGV 100
RIWDANGSRD FLDSLGFSAR QEGDLGPVYG FQWRHFGAEY KDMDSDYSGQ 150
GVDQLQKVID TIKTNPDDRR IIMCAWNPKD LPLMALPPCH ALCQFYVVNG 200
ELSCQLYQRS GDMGLGVPFN IASYALLTYM IAHITGLQPG DFVHTLGDAH 250
IYLNHIEPLK IQLQREPRPF PKLKILRKVE TIDDFKVEDF QIEGYNPHPT 300
IKMEMAV 307
Length:307
Mass (Da):34,958
Last modified:April 1, 1988 - v1
Checksum:iE4930618C487FD5E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13019 mRNA. Translation: AAA40439.1.
M13352
, J02617, M13347, M13348, M13349, M13350, M13351 Genomic DNA. Translation: AAA40444.1.
X14489 mRNA. Translation: CAA32651.1.
CCDSiCCDS19154.1.
PIRiA26323. YXMST.
RefSeqiNP_067263.1. NM_021288.4.
UniGeneiMm.268395.

Genome annotation databases

EnsembliENSMUST00000026846; ENSMUSP00000026846; ENSMUSG00000025747.
GeneIDi22171.
KEGGimmu:22171.
UCSCiuc008wux.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13019 mRNA. Translation: AAA40439.1 .
M13352
, J02617 , M13347 , M13348 , M13349 , M13350 , M13351 Genomic DNA. Translation: AAA40444.1 .
X14489 mRNA. Translation: CAA32651.1 .
CCDSi CCDS19154.1.
PIRi A26323. YXMST.
RefSeqi NP_067263.1. NM_021288.4.
UniGenei Mm.268395.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3IHI X-ray 1.94 A/B 1-307 [» ]
4E5O X-ray 1.70 A/B/C/D/E/F 1-307 [» ]
4EB4 X-ray 1.74 A/B/C/D 1-307 [» ]
4EIN X-ray 1.75 A/B 1-307 [» ]
4EZ8 X-ray 1.17 A 1-307 [» ]
ProteinModelPortali P07607.
SMRi P07607. Positions 21-307.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P07607.
ChEMBLi CHEMBL3160.
GuidetoPHARMACOLOGYi 2642.

PTM databases

PhosphoSitei P07607.

2D gel databases

REPRODUCTION-2DPAGE P07607.

Proteomic databases

PaxDbi P07607.
PRIDEi P07607.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026846 ; ENSMUSP00000026846 ; ENSMUSG00000025747 .
GeneIDi 22171.
KEGGi mmu:22171.
UCSCi uc008wux.2. mouse.

Organism-specific databases

CTDi 7298.
MGIi MGI:98878. Tyms.

Phylogenomic databases

eggNOGi COG0207.
HOGENOMi HOG000257899.
HOVERGENi HBG001934.
InParanoidi P07607.
KOi K00560.
OMAi DVHLYSN.
PhylomeDBi P07607.
TreeFami TF353027.

Enzyme and pathway databases

UniPathwayi UPA00575 .
Reactomei REACT_198608. G1/S-Specific Transcription.
REACT_206830. E2F mediated regulation of DNA replication.

Miscellaneous databases

EvolutionaryTracei P07607.
NextBioi 302116.
PROi P07607.
SOURCEi Search...

Gene expression databases

ArrayExpressi P07607.
Bgeei P07607.
CleanExi MM_TYMS.
Genevestigatori P07607.

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 1 hit.
PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of a cDNA for mouse thymidylate synthase reveals striking similarity with the prokaryotic enzyme."
    Perryman S.M., Rossana C., Deng T., Vanin E.F., Johnson L.F.
    Mol. Biol. Evol. 3:313-321(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure of the gene for mouse thymidylate synthase. Locations of introns and multiple transcriptional start sites."
    Deng T., Li D., Jenh C.-H., Johnson L.F.
    J. Biol. Chem. 261:16000-16005(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Thymidylate synthase gene expression is stimulated by some (but not all) introns."
    Deng T., Li Y., Johnson L.F.
    Nucleic Acids Res. 17:645-658(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 236-265.

Entry informationi

Entry nameiTYSY_MOUSE
AccessioniPrimary (citable) accession number: P07607
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: September 3, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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