ID   TYSY_BPPHT              Reviewed;         279 AA.
AC   P07606;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   16-JUN-2009, entry version 60.
DE   RecName: Full=Thymidylate synthase;
DE            Short=TSase;
DE            Short=TS;
DE            EC=2.1.1.45;
GN   Name=thyP3;
OS   Bacillus phage phi3T (Bacteriophage phi-3T).
OC   Viruses; dsDNA viruses, no RNA stage; Caudovirales; Siphoviridae;
OC   Lambda-like viruses; unclassified Lambda-like viruses.
OX   NCBI_TaxID=10736;
OH   NCBI_TaxID=1423; Bacillus subtilis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=85232079; PubMed=3924741; DOI=10.1016/0378-1119(85)90142-8;
RA   Kenny E., Atkinson T., Hartley B.S.;
RT   "Nucleotide sequence of the thymidylate synthetase gene (thyP3) from
RT   the Bacillus subtilis phage phi 3T.";
RL   Gene 34:335-342(1985).
CC   -!- FUNCTION: Provides the sole de novo source of dTMP for DNA
CC       biosynthesis.
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + dUMP =
CC       dihydrofolate + dTMP.
CC   -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the thymidylate synthase family.
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DR   EMBL; M10122; AAA32353.1; -; Genomic_DNA.
DR   PIR; A22800; SYBP3T.
DR   HSSP; P42326; 1BSP.
DR   SMR; P07606; 2-279.
DR   GO; GO:0004799; F:thymidylate synthase activity; IEA:EC.
DR   GO; GO:0006231; P:dTMP biosynthetic process; IEA:InterPro.
DR   InterPro; IPR000398; Thymidylat_synth_C.
DR   Gene3D; G3DSA:3.30.572.10; Thymidylat_synth_C; 1.
DR   PANTHER; PTHR11549:SF2; Thymidylat_synth_C; 1.
DR   Pfam; PF00303; Thymidylat_synt; 1.
DR   PRINTS; PR00108; THYMDSNTHASE.
DR   ProDom; PD001180; Thymidylat_synth; 1.
DR   TIGRFAMs; TIGR03284; thym_sym; 1.
DR   PROSITE; PS00091; THYMIDYLATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Nucleotide biosynthesis; Transferase.
FT   CHAIN         1    279       Thymidylate synthase.
FT                                /FTId=PRO_0000141060.
FT   ACT_SITE    161    161       By similarity.
SQ   SEQUENCE   279 AA;  32785 MW;  F8FC67BD2DF7A727 CRC64;
     MTQFDKQYNS IIKDIINNGI SDEEFDVRTK WDSDGTPAHT LSVMSKQMRF DNSEVPILTT
     KKVAWKTAIK ELLWIWQLKS NDVTELNKMG VHIWDQWKQE DGTIGHAYGF QLGKKNRSLN
     GEKVDQVDYL LHQLKNNPSS RRHITMLWNP DDLDAMALTP CVYETQWYVK QGKLHLEVRA
     RSNDMALGNP FNVFQYNVLQ RMIAQVTGYE LGEYIFNIGD CHVYTRHIDN LKIQMEREQF
     EAPELWINPE VKDFYNFTVD DFKLINYKHG DKLLFEVAV
//