Reviewed, UniProtKB/Swiss-Prot P07606 (TYSY_BPPHT)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TSase
      Short name=TS
    EC=2.1.1.45

Gene names

Name:

thyP3

Organism

Bacillus phage phi3T (Bacteriophage phi-3T)

Taxonomic identifier

10736 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambda-like viruses › unclassified Lambda-like viruses

Virus host

Bacillus subtilis [TaxID: 1423]

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
Pathway	Pyrimidine metabolism; dTTP biosynthesis.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the thymidylate synthase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Thymidylate synthase		PRO_0000141060
Sites
	Active site	161	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07606-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: F8FC67BD2DF7A727	FASTA	279	32,785
	10 20 30 40 50 60 MTQFDKQYNS IIKDIINNGI SDEEFDVRTK WDSDGTPAHT LSVMSKQMRF DNSEVPILTT 70 80 90 100 110 120 KKVAWKTAIK ELLWIWQLKS NDVTELNKMG VHIWDQWKQE DGTIGHAYGF QLGKKNRSLN 130 140 150 160 170 180 GEKVDQVDYL LHQLKNNPSS RRHITMLWNP DDLDAMALTP CVYETQWYVK QGKLHLEVRA 190 200 210 220 230 240 RSNDMALGNP FNVFQYNVLQ RMIAQVTGYE LGEYIFNIGD CHVYTRHIDN LKIQMEREQF 250 260 270 EAPELWINPE VKDFYNFTVD DFKLINYKHG DKLLFEVAV

References

[1]	"Nucleotide sequence of the thymidylate synthetase gene (thyP3) from the Bacillus subtilis phage phi 3T." Kenny E., Atkinson T., Hartley B.S. Gene 34:335-342(1985) [PubMed: 3924741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M10122 Genomic DNA. Translation: AAA32353.1.
PIR	SYBP3T. A22800.
3D structure databases
HSSP	HSSP built from PDB template 1BSP based on UniProtKB P42326.
SMR	P07606. Positions 2-279.
ModBase	Search...
Family and domain databases
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BPPHT

Accession

Primary (citable) accession number: P07606

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)