ID   KITH_RFVKA              Reviewed;         178 AA.
AC   P07605; Q9Q910;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 2.
DT   08-NOV-2023, entry version 81.
DE   RecName: Full=Thymidine kinase;
DE            EC=2.7.1.21;
GN   Name=TK; ORFNames=s061R;
OS   Rabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain
OS   Kasza)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Leporipoxvirus;
OC   Rabbit fibroma virus.
OX   NCBI_TaxID=10272;
OH   NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3023681; DOI=10.1128/jvi.60.3.920-927.1986;
RA   Upton C., McFadden G.;
RT   "Identification and nucleotide sequence of the thymidine kinase gene of
RT   Shope fibroma virus.";
RL   J. Virol. 60:920-927(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3014722; DOI=10.1016/0042-6822(86)90134-0;
RA   Upton C., McFadden G.;
RT   "Tumorigenic poxviruses: analysis of viral DNA sequences implicated in the
RT   tumorigenicity of Shope fibroma virus and malignant rabbit virus.";
RL   Virology 152:308-321(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1660196; DOI=10.1016/0042-6822(91)90529-k;
RA   Strayer D.S., Jerng H.H., O'Connor K.;
RT   "Sequence and analysis of a portion of the genomes of Shope fibroma virus
RT   and malignant rabbit fibroma virus that is important for viral replication
RT   in lymphocytes.";
RL   Virology 185:585-595(1991).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10562495; DOI=10.1006/viro.1999.0002;
RA   Willer D.O., McFadden G., Evans D.H.;
RT   "The complete genome sequence of shope (Rabbit) fibroma virus.";
RL   Virology 264:319-343(1999).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + thymidine = ADP + dTMP + H(+); Xref=Rhea:RHEA:19129,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17748, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:63528, ChEBI:CHEBI:456216; EC=2.7.1.21;
CC   -!- SIMILARITY: Belongs to the thymidine kinase family. {ECO:0000305}.
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DR   EMBL; M14493; AAA47227.1; -; Genomic_DNA.
DR   EMBL; AF170722; AAF17943.1; -; Genomic_DNA.
DR   PIR; A26102; KIVZSF.
DR   RefSeq; NP_051950.1; NC_001266.1.
DR   SMR; P07605; -.
DR   GeneID; 1486904; -.
DR   KEGG; vg:1486904; -.
DR   Proteomes; UP000000868; Segment.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.60.20; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001267; Thymidine_kinase.
DR   InterPro; IPR020633; Thymidine_kinase_CS.
DR   PANTHER; PTHR11441; THYMIDINE KINASE; 1.
DR   PANTHER; PTHR11441:SF0; THYMIDINE KINASE, CYTOSOLIC; 1.
DR   Pfam; PF00265; TK; 1.
DR   PIRSF; PIRSF035805; TK_cell; 1.
DR   SUPFAM; SSF57716; Glucocorticoid receptor-like (DNA-binding domain); 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00603; TK_CELLULAR_TYPE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA synthesis; Kinase; Metal-binding; Nucleotide-binding;
KW   Reference proteome; Transferase; Zinc.
FT   CHAIN           1..178
FT                   /note="Thymidine kinase"
FT                   /id="PRO_0000174935"
FT   ACT_SITE        85
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   BINDING         13..20
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         140
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         143
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         159..163
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         172
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         175
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        2..3
FT                   /note="Missing (in Ref. 1; AAA47227)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        102
FT                   /note="A -> R (in Ref. 1; AAA47227)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   178 AA;  19896 MW;  51A7775471A99D53 CRC64;
     MTMYGGHIHL IIGPMFAGKS TELIRLVRRY QIAKHKCLVV KYEKDIRYGN GVCTHDNMSI
     TAVCTPSLDK IDSVAENAEV IGIDEGQFFP NIATFCERMA NAGKVLIVAA LDGTFQRKPF
     SNISELIPLA ENVTKLNAVC MYCYKNGSFS KRLGDKMEIE VIGGSDKYKS VCRKCYFF
//