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Protein

Transcriptional regulatory protein TyrR

Gene

tyrR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in transcriptional regulation of aromatic amino acid biosynthesis and transport. Modulates the expression of at least 8 unlinked operons. Seven of these operons are regulated in response to changes in the concentration of the three aromatic amino acids (phenylalanine, tyrosine and tryptophan). These amino acids are suggested to act as co-effectors which bind to the TyrR protein to form an active regulatory protein. In most cases TyrR causes negative regulation, but positive effects on the tyrP gene have been observed at high phenylalanine concentrations.

Miscellaneous

The tyrR gene is autogenously regulated by a mechanism that gives similar rates of expression of tyrR irrespective of concentration of the aromatic amino acids.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi234 – 241ATPPROSITE-ProRule annotation8
Nucleotide bindingi290 – 299ATPPROSITE-ProRule annotation10
DNA bindingi483 – 502H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

  • ATP binding Source: EcoCyc
  • DNA binding Source: EcoCyc
  • transcription factor binding Source: InterPro

GO - Biological processi

  • aromatic compound catabolic process Source: UniProtKB-KW
  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • phosphorelay signal transduction system Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processAromatic hydrocarbons catabolism, Transcription, Transcription regulation, Two-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00413

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein TyrR
Gene namesi
Name:tyrR
Ordered Locus Names:b1323, JW1316
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11042 tyrR

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000813391 – 513Transcriptional regulatory protein TyrRAdd BLAST513

Proteomic databases

EPDiP07604
PaxDbiP07604
PRIDEiP07604

Interactioni

Subunit structurei

Homodimer. In presence of tyrosine (to the lesser extent, phenylalanine or tryptophan) and excess of ATP it undergoes a ligand-induced hexamerization. It is suggested that the hexameric form of TyrR is the active repressing species, interacting with two or three tyrR boxes in the targeted regulatory DNA.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
aceFP069592EBI-559274,EBI-542707

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4260150, 11 interactors
DIPiDIP-11062N
IntActiP07604, 4 interactors
STRINGi316385.ECDH10B_1442

Structurei

Secondary structure

1513
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi13 – 23Combined sources11
Beta strandi28 – 34Combined sources7
Turni35 – 37Combined sources3
Beta strandi38 – 43Combined sources6
Helixi48 – 60Combined sources13
Beta strandi64 – 71Combined sources8
Turni74 – 76Combined sources3
Helixi77 – 88Combined sources12
Beta strandi93 – 96Combined sources4
Beta strandi101 – 105Combined sources5
Helixi107 – 113Combined sources7
Helixi117 – 120Combined sources4
Helixi125 – 127Combined sources3
Helixi134 – 139Combined sources6
Beta strandi146 – 152Combined sources7
Beta strandi155 – 164Combined sources10
Turni165 – 168Combined sources4
Beta strandi169 – 176Combined sources8
Helixi178 – 185Combined sources8
Turni186 – 188Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JHEX-ray2.30A/B/C/D1-190[»]
ProteinModelPortaliP07604
SMRiP07604
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07604

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 72ACTPROSITE-ProRule annotationAdd BLAST71
Domaini78 – 149PASPROSITE-ProRule annotationAdd BLAST72
Domaini206 – 428Sigma-54 factor interactionPROSITE-ProRule annotationAdd BLAST223

Phylogenomic databases

eggNOGiENOG4108JU8 Bacteria
COG3283 LUCA
HOGENOMiHOG000058487
InParanoidiP07604
KOiK03721
OMAiCQNRIGI
PhylomeDBiP07604

Family and domain databases

CDDicd00130 PAS, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR002912 ACT_dom
IPR009057 Homeobox-like_sf
IPR030828 HTH_TypR
IPR027417 P-loop_NTPase
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR002078 Sigma_54_int
IPR025662 Sigma_54_int_dom_ATP-bd_1
IPR025943 Sigma_54_int_dom_ATP-bd_2
IPR025944 Sigma_54_int_dom_CS
PfamiView protein in Pfam
PF13188 PAS_8, 1 hit
PF00158 Sigma54_activat, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00091 PAS, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF52540 SSF52540, 1 hit
SSF55785 SSF55785, 1 hit
TIGRFAMsiTIGR04381 HTH_TypR, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit
PS50112 PAS, 1 hit
PS00675 SIGMA54_INTERACT_1, 1 hit
PS00676 SIGMA54_INTERACT_2, 1 hit
PS00688 SIGMA54_INTERACT_3, 1 hit
PS50045 SIGMA54_INTERACT_4, 1 hit

Sequencei

Sequence statusi: Complete.

P07604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLEVFCEDR LGLTRELLDL LVLRGIDLRG IEIDPIGRIY LNFAELEFES
60 70 80 90 100
FSSLMAEIRR IAGVTDVRTV PWMPSEREHL ALSALLEALP EPVLSVDMKS
110 120 130 140 150
KVDMANPASC QLFGQKLDRL RNHTAAQLIN GFNFLRWLES EPQDSHNEHV
160 170 180 190 200
VINGQNFLME ITPVYLQDEN DQHVLTGAVV MLRSTIRMGR QLQNVAAQDV
210 220 230 240 250
SAFSQIVAVS PKMKHVVEQA QKLAMLSAPL LITGDTGTGK DLFAYACHQA
260 270 280 290 300
SPRAGKPYLA LNCASIPEDA VESELFGHAP EGKKGFFEQA NGGSVLLDEI
310 320 330 340 350
GEMSPRMQAK LLRFLNDGTF RRVGEDHEVH VDVRVICATQ KNLVELVQKG
360 370 380 390 400
MFREDLYYRL NVLTLNLPPL RDCPQDIMPL TELFVARFAD EQGVPRPKLA
410 420 430 440 450
ADLNTVLTRY AWPGNVRQLK NAIYRALTQL DGYELRPQDI LLPDYDAATV
460 470 480 490 500
AVGEDAMEGS LDEITSRFER SVLTQLYRNY PSTRKLAKRL GVSHTAIANK
510
LREYGLSQKK NEE
Length:513
Mass (Da):57,656
Last modified:November 1, 1991 - v2
Checksum:iB580A401C3A27866
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12114 Genomic DNA Translation: AAA24706.1
U00096 Genomic DNA Translation: AAC74405.1
AP009048 Genomic DNA Translation: BAA14905.1
PIRiA47086 RGECAY
RefSeqiNP_415839.1, NC_000913.3
WP_001300658.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74405; AAC74405; b1323
BAA14905; BAA14905; BAA14905
GeneIDi945879
KEGGiecj:JW1316
eco:b1323
PATRICifig|1411691.4.peg.955

Similar proteinsi

Entry informationi

Entry nameiTYRR_ECOLI
AccessioniPrimary (citable) accession number: P07604
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1991
Last modified: March 28, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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