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Protein

Alpha-amylase/subtilisin inhibitor

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein inhibits independently subtilisin and alpha-amylase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei89 – 902Reactive bondBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Alpha-amylase inhibitor, Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI03.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase/subtilisin inhibitor
Alternative name(s):
BASI
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22221 PublicationAdd
BLAST
Chaini23 – 203181Alpha-amylase/subtilisin inhibitorPRO_0000016931Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi65 ↔ 1121 Publication
Disulfide bondi166 ↔ 1701 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

DIPiDIP-6098N.

Structurei

Secondary structure

1
203
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi42 – 487Combined sources
Helixi49 – 513Combined sources
Beta strandi55 – 584Combined sources
Beta strandi61 – 644Combined sources
Beta strandi68 – 714Combined sources
Beta strandi82 – 887Combined sources
Beta strandi92 – 954Combined sources
Beta strandi102 – 1065Combined sources
Beta strandi125 – 1273Combined sources
Beta strandi130 – 1323Combined sources
Helixi144 – 1463Combined sources
Beta strandi148 – 1525Combined sources
Beta strandi161 – 17212Combined sources
Beta strandi174 – 1763Combined sources
Beta strandi179 – 1824Combined sources
Beta strandi184 – 1918Combined sources
Beta strandi194 – 1996Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AVAX-ray1.90C/D23-203[»]
2IWTX-ray2.30B22-203[»]
3BX1X-ray1.85C/D23-203[»]
ProteinModelPortaliP07596.
SMRiP07596. Positions 23-203.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07596.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

CDDicd00178. STI. 1 hit.
InterProiIPR011065. Kunitz_inhibitor_ST1-like.
IPR002160. Prot_inh_Kunz-lg.
[Graphical view]
PfamiPF00197. Kunitz_legume. 1 hit.
[Graphical view]
PRINTSiPR00291. KUNITZINHBTR.
SMARTiSM00452. STI. 1 hit.
[Graphical view]
SUPFAMiSSF50386. SSF50386. 1 hit.
PROSITEiPS00283. SOYBEAN_KUNITZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSRRAGSSS SPLFWPAPPS RAADPPPVHD TDGHELRADA NYYVLSANRA
60 70 80 90 100
HGGGLTMAPG HGRHCPLFVS QDPNGQHDGF PVRITPYGVA PSDKIIRLST
110 120 130 140 150
DVRISFRAYT TCLQSTEWHI DSELAAGRRH VITGPVKDPS PSGRENAFRI
160 170 180 190 200
EKYSGAEVHE YKLMSCGDWC QDLGVFRDLK GGAWFLGATE PYHVVVFKKA

PPA
Length:203
Mass (Da):22,164
Last modified:January 1, 1990 - v2
Checksum:i4E3F9F789E47970B
GO

Sequence cautioni

The sequence CAA78305 differs from that shown. Reason: Frameshift at positions 8 and 23. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241D → K AA sequence (PubMed:11271488).Curated
Sequence conflicti29 – 291H → K AA sequence (PubMed:11271488).Curated
Sequence conflicti32 – 321D → A AA sequence (PubMed:7639717).Curated
Sequence conflicti32 – 321D → G AA sequence (PubMed:11271488).Curated
Sequence conflicti154 – 1541S → H AA sequence (Ref. 3) Curated
Sequence conflicti159 – 1591H → S AA sequence (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16276 mRNA. Translation: CAA34352.1.
Z12961 Genomic DNA. Translation: CAA78305.1. Frameshift.
PIRiS04860.
S22639.
UniGeneiHv.341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16276 mRNA. Translation: CAA34352.1.
Z12961 Genomic DNA. Translation: CAA78305.1. Frameshift.
PIRiS04860.
S22639.
UniGeneiHv.341.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AVAX-ray1.90C/D23-203[»]
2IWTX-ray2.30B22-203[»]
3BX1X-ray1.85C/D23-203[»]
ProteinModelPortaliP07596.
SMRiP07596. Positions 23-203.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6098N.

Protein family/group databases

MEROPSiI03.004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP07596.

Family and domain databases

CDDicd00178. STI. 1 hit.
InterProiIPR011065. Kunitz_inhibitor_ST1-like.
IPR002160. Prot_inh_Kunz-lg.
[Graphical view]
PfamiPF00197. Kunitz_legume. 1 hit.
[Graphical view]
PRINTSiPR00291. KUNITZINHBTR.
SMARTiSM00452. STI. 1 hit.
[Graphical view]
SUPFAMiSSF50386. SSF50386. 1 hit.
PROSITEiPS00283. SOYBEAN_KUNITZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIAAS_HORVU
AccessioniPrimary (citable) accession number: P07596
Secondary accession number(s): P82941, Q40023, Q7M223
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 1, 1990
Last modified: September 7, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.