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Protein

Alpha-amylase/subtilisin inhibitor

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein inhibits independently subtilisin and alpha-amylase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei89 – 90Reactive bondBy similarity2

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Alpha-amylase inhibitor, Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI03.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase/subtilisin inhibitor
Alternative name(s):
BASI
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000001693123 – 203Alpha-amylase/subtilisin inhibitorAdd BLAST181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi65 ↔ 1121 Publication
Disulfide bondi166 ↔ 1701 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

DIPiDIP-6098N.

Structurei

Secondary structure

1203
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 48Combined sources7
Helixi49 – 51Combined sources3
Beta strandi55 – 58Combined sources4
Beta strandi61 – 64Combined sources4
Beta strandi68 – 71Combined sources4
Beta strandi82 – 88Combined sources7
Beta strandi92 – 95Combined sources4
Beta strandi102 – 106Combined sources5
Beta strandi125 – 127Combined sources3
Beta strandi130 – 132Combined sources3
Helixi144 – 146Combined sources3
Beta strandi148 – 152Combined sources5
Beta strandi161 – 172Combined sources12
Beta strandi174 – 176Combined sources3
Beta strandi179 – 182Combined sources4
Beta strandi184 – 191Combined sources8
Beta strandi194 – 199Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AVAX-ray1.90C/D23-203[»]
2IWTX-ray2.30B22-203[»]
3BX1X-ray1.85C/D23-203[»]
ProteinModelPortaliP07596.
SMRiP07596.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07596.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

CDDicd00178. STI. 1 hit.
InterProiIPR011065. Kunitz_inhibitor_ST1-like.
IPR002160. Prot_inh_Kunz-lg.
[Graphical view]
PfamiPF00197. Kunitz_legume. 1 hit.
[Graphical view]
PRINTSiPR00291. KUNITZINHBTR.
SMARTiSM00452. STI. 1 hit.
[Graphical view]
SUPFAMiSSF50386. SSF50386. 1 hit.
PROSITEiPS00283. SOYBEAN_KUNITZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSRRAGSSS SPLFWPAPPS RAADPPPVHD TDGHELRADA NYYVLSANRA
60 70 80 90 100
HGGGLTMAPG HGRHCPLFVS QDPNGQHDGF PVRITPYGVA PSDKIIRLST
110 120 130 140 150
DVRISFRAYT TCLQSTEWHI DSELAAGRRH VITGPVKDPS PSGRENAFRI
160 170 180 190 200
EKYSGAEVHE YKLMSCGDWC QDLGVFRDLK GGAWFLGATE PYHVVVFKKA

PPA
Length:203
Mass (Da):22,164
Last modified:January 1, 1990 - v2
Checksum:i4E3F9F789E47970B
GO

Sequence cautioni

The sequence CAA78305 differs from that shown. Reason: Frameshift at positions 8 and 23.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24D → K AA sequence (PubMed:11271488).Curated1
Sequence conflicti29H → K AA sequence (PubMed:11271488).Curated1
Sequence conflicti32D → A AA sequence (PubMed:7639717).Curated1
Sequence conflicti32D → G AA sequence (PubMed:11271488).Curated1
Sequence conflicti154S → H AA sequence (Ref. 3) Curated1
Sequence conflicti159H → S AA sequence (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16276 mRNA. Translation: CAA34352.1.
Z12961 Genomic DNA. Translation: CAA78305.1. Frameshift.
PIRiS04860.
S22639.
UniGeneiHv.341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16276 mRNA. Translation: CAA34352.1.
Z12961 Genomic DNA. Translation: CAA78305.1. Frameshift.
PIRiS04860.
S22639.
UniGeneiHv.341.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AVAX-ray1.90C/D23-203[»]
2IWTX-ray2.30B22-203[»]
3BX1X-ray1.85C/D23-203[»]
ProteinModelPortaliP07596.
SMRiP07596.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6098N.

Protein family/group databases

MEROPSiI03.004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP07596.

Family and domain databases

CDDicd00178. STI. 1 hit.
InterProiIPR011065. Kunitz_inhibitor_ST1-like.
IPR002160. Prot_inh_Kunz-lg.
[Graphical view]
PfamiPF00197. Kunitz_legume. 1 hit.
[Graphical view]
PRINTSiPR00291. KUNITZINHBTR.
SMARTiSM00452. STI. 1 hit.
[Graphical view]
SUPFAMiSSF50386. SSF50386. 1 hit.
PROSITEiPS00283. SOYBEAN_KUNITZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIAAS_HORVU
AccessioniPrimary (citable) accession number: P07596
Secondary accession number(s): P82941, Q40023, Q7M223
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 1, 1990
Last modified: November 2, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.