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P07596 (IAAS_HORVU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alpha-amylase/subtilisin inhibitor
Alternative name(s):
BASI
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length203 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein inhibits independently subtilisin and alpha-amylase.

Sequence similarities

Belongs to the protease inhibitor I3 (leguminous Kunitz-type inhibitor) family. [View classification]

Sequence caution

The sequence CAA78305.1 differs from that shown. Reason: Frameshift at positions 8 and 23.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Ref.3
Chain23 – 203181Alpha-amylase/subtilisin inhibitor
PRO_0000016931

Sites

Site89 – 902Reactive bond By similarity

Amino acid modifications

Disulfide bond65 ↔ 112
Disulfide bond166 ↔ 170

Experimental info

Sequence conflict241D → K AA sequence Ref.4
Sequence conflict291H → K AA sequence Ref.4
Sequence conflict321D → A AA sequence Ref.5
Sequence conflict321D → G AA sequence Ref.4
Sequence conflict1541S → H AA sequence Ref.3
Sequence conflict1591H → S AA sequence Ref.3

Secondary structure

1 .................................. 203
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07596 [UniParc].

Last modified January 1, 1990. Version 2.
Checksum: 4E3F9F789E47970B

FASTA20322,164
        10         20         30         40         50         60 
MGSRRAGSSS SPLFWPAPPS RAADPPPVHD TDGHELRADA NYYVLSANRA HGGGLTMAPG 

        70         80         90        100        110        120 
HGRHCPLFVS QDPNGQHDGF PVRITPYGVA PSDKIIRLST DVRISFRAYT TCLQSTEWHI 

       130        140        150        160        170        180 
DSELAAGRRH VITGPVKDPS PSGRENAFRI EKYSGAEVHE YKLMSCGDWC QDLGVFRDLK 

       190        200 
GGAWFLGATE PYHVVVFKKA PPA

« Hide

References

[1]"The bifunctional alpha-amylase/subtilisin inhibitor of barley: nucleotide sequence and patterns of seed-specific expression."
Leah R., Mundy J.
Plant Mol. Biol. 12:673-682(1989)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Seed.
[2]"Genomic sequence of bifunctional alpha-amylase/subtilisin inhibitor from barley."
McKinnon G.E., Henry R.J.
Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Betzes.
[3]"Complete amino acid sequence of the alpha-amylase/subtilisin inhibitor from barley."
Svendsen I., Hejgaard J., Mundy J.
Carlsberg Res. Commun. 51:43-50(1986)
Cited for: PROTEIN SEQUENCE OF 23-203.
[4]"Separation and characterization of basic barley seed proteins."
Kristoffersen H.E., Flengsrud R.
Electrophoresis 21:3693-3700(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-42.
Strain: cv. Bomi.
Tissue: Starchy endosperm.
[5]"Arg-27, Arg-127 and Arg-155 in the beta-trefoil protein barley alpha-amylase/subtilisin inhibitor are interface residues in the complex with barley alpha-amylase 2."
Rodenburg K.W., Varallyay E., Svendsen I., Svensson B.
Biochem. J. 309:969-976(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-98; 119-132; 149-153; 162-164 AND 177-183.
Strain: cv. Piggy.
Tissue: Seed.
[6]"Barley alpha-amylase bound to its endogenous protein inhibitor BASI: crystal structure of the complex at 1.9-A resolution."
Vallee F., Kadziola A., Bourne Y., Juy M., Rodenburg K.W., Svensson B., Haser R.
Structure 6:649-659(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF COMPLEX WITH AMY2.
Strain: cv. Piggy.
Tissue: Seed.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X16276 mRNA. Translation: CAA34352.1.
Z12961 Genomic DNA. Translation: CAA78305.1. Frameshift.
PIRS04860.
S22639.
UniGeneHv.341.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AVAX-ray1.90C/D23-203[»]
2IWTX-ray2.30B22-203[»]
3BX1X-ray1.85C/D23-203[»]
ProteinModelPortalP07596.
SMRP07596. Positions 23-203.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-6098N.

Protein family/group databases

MEROPSI03.004.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP07596.

Gene expression databases

GenevestigatorP07596.

Family and domain databases

InterProIPR011065. Kunitz_inhibitor_ST1-like.
IPR002160. Prot_inh_Kunz-lg.
[Graphical view]
PfamPF00197. Kunitz_legume. 1 hit.
[Graphical view]
PRINTSPR00291. KUNITZINHBTR.
SMARTSM00452. STI. 1 hit.
[Graphical view]
SUPFAMSSF50386. Kunitz_like. 1 hit.
PROSITEPS00283. SOYBEAN_KUNITZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP07596.

Entry information

Entry nameIAAS_HORVU
AccessionPrimary (citable) accession number: P07596
Secondary accession number(s): P82941, Q40023, Q7M223
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 1, 1990
Last modified: April 3, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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