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Protein

Astacin

Gene
N/A
Organism
Astacus astacus (Noble crayfish) (Astacus fluviatilis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protease prefers to cleave in front of small aliphatic residues (P1'). The presence of Lys or Arg in the P1 and P2 position yields high-turnover substrates. In the P3 position the enzyme prefers Pro > Val > Leu > Ala > Gly.1 Publication

Catalytic activityi

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.2 Publications

Cofactori

Zn2+3 PublicationsNote: Binds 1 zinc ion per subunit.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi141Zinc; catalyticPROSITE-ProRule annotation2 Publications1
Active sitei1421 Publication1
Metal bindingi145Zinc; catalyticPROSITE-ProRule annotation2 Publications1
Metal bindingi151Zinc; catalyticPROSITE-ProRule annotation2 Publications1

GO - Molecular functioni

  • aspartic-type peptidase activity Source: UniProtKB
  • glutamic-type peptidase activity Source: UniProtKB
  • metalloendopeptidase activity Source: InterPro
  • peptidase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cell adhesion Source: UniProtKB
  • fertilization Source: UniProtKB
  • negative regulation of binding of sperm to zona pellucida Source: UniProtKB
  • positive regulation of protein processing Source: UniProtKB
  • prevention of polyspermy Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKP07584.

Protein family/group databases

MEROPSiM12.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Astacin (EC:3.4.24.212 Publications)
Alternative name(s):
Crayfish small molecule proteinase
OrganismiAstacus astacus (Noble crayfish) (Astacus fluviatilis)
Taxonomic identifieri6715 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaCrustaceaMalacostracaEumalacostracaEucaridaDecapodaPleocyemataAstacideaAstacoideaAstacidaeAstacus

Subcellular locationi

GO - Cellular componenti

  • cortical granule Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
PropeptideiPRO_000002887216 – 49Activation peptide1 PublicationAdd BLAST34
ChainiPRO_000002887350 – 249Astacin1 PublicationAdd BLAST200
PropeptideiPRO_0000028874250 – 2512

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi91 ↔ 2474 Publications
Disulfide bondi113 ↔ 1334 Publications

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1251
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 27Combined sources7
Turni34 – 36Combined sources3
Beta strandi46 – 48Combined sources3
Beta strandi51 – 53Combined sources3
Helixi55 – 57Combined sources3
Helixi60 – 62Combined sources3
Beta strandi63 – 70Combined sources8
Helixi73 – 89Combined sources17
Beta strandi93 – 96Combined sources4
Beta strandi101 – 118Combined sources18
Beta strandi121 – 128Combined sources8
Turni130 – 133Combined sources4
Helixi136 – 147Combined sources12
Helixi152 – 154Combined sources3
Helixi158 – 160Combined sources3
Beta strandi162 – 164Combined sources3
Helixi166 – 168Combined sources3
Helixi174 – 177Combined sources4
Beta strandi181 – 184Combined sources4
Turni200 – 203Combined sources4
Turni205 – 209Combined sources5
Beta strandi212 – 217Combined sources6
Helixi225 – 227Combined sources3
Helixi233 – 242Combined sources10
Helixi244 – 249Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ASTX-ray1.80A50-249[»]
1IAAX-ray1.90A50-249[»]
1IABX-ray1.79A50-249[»]
1IACX-ray2.10A50-249[»]
1IADX-ray2.30A50-249[»]
1IAEX-ray1.83A50-249[»]
1QJIX-ray2.14A50-249[»]
1QJJX-ray1.86A50-249[»]
3LQ0X-ray1.45A16-250[»]
ProteinModelPortaliP07584.
SMRiP07584.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07584.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK08076.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
[Graphical view]
PRINTSiPR00480. ASTACIN.
SMARTiSM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQCAVLLVLL GVVAASPIIP EAARALYYND GMFEGDIKLR AGRQPARVGA
60 70 80 90 100
AILGDEYLWS GGVIPYTFAG VSGADQSAIL SGMQELEEKT CIRFVPRTTE
110 120 130 140 150
SDYVEIFTSG SGCWSYVGRI SGAQQVSLQA NGCVYHGTII HELMHAIGFY
160 170 180 190 200
HEHTRMDRDN YVTINYQNVD PSMTSNFDID TYSRYVGEDY QYYSIMHYGK
210 220 230 240 250
YSFSIQWGVL ETIVPLQNGI DLTDPYDKAH MLQTDANQIN NLYTNECSLR

H
Length:251
Mass (Da):28,093
Last modified:October 1, 1996 - v2
Checksum:i00E76C704334343B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95684 Genomic DNA. Translation: CAA64981.1.
PIRiA58830. HYCY.

Genome annotation databases

KEGGiag:CAA64981.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95684 Genomic DNA. Translation: CAA64981.1.
PIRiA58830. HYCY.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ASTX-ray1.80A50-249[»]
1IAAX-ray1.90A50-249[»]
1IABX-ray1.79A50-249[»]
1IACX-ray2.10A50-249[»]
1IADX-ray2.30A50-249[»]
1IAEX-ray1.83A50-249[»]
1QJIX-ray2.14A50-249[»]
1QJJX-ray1.86A50-249[»]
3LQ0X-ray1.45A16-250[»]
ProteinModelPortaliP07584.
SMRiP07584.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM12.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA64981.

Phylogenomic databases

KOiK08076.

Enzyme and pathway databases

SABIO-RKP07584.

Miscellaneous databases

EvolutionaryTraceiP07584.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
[Graphical view]
PRINTSiPR00480. ASTACIN.
SMARTiSM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASTA_ASTAS
AccessioniPrimary (citable) accession number: P07584
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.