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Protein

Gag polyprotein

Gene

gag

Organism
Mason-Pfizer monkey virus (MPMV) (Simian Mason-Pfizer virus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Matrix protein p10: Matrix protein.
Nucleocapsid protein p14: Nucleocapsid protein.
Capsid protein p27: capsid protein.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri547 – 564CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri576 – 593CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Keywords - Ligandi

Metal-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 6 chains:
Gene namesi
Name:gag
OrganismiMason-Pfizer monkey virus (MPMV) (Simian Mason-Pfizer virus)
Taxonomic identifieri11855 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeBetaretrovirus
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]
Proteomesi
  • UP000008870 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • host cell cytoplasm Source: UniProtKB
  • viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostBy similarity
ChainiPRO_00000409402 – 100Matrix protein p10Add BLAST99
ChainiPRO_0000040941101 – 216Core phosphoprotein pp24Add BLAST116
ChainiPRO_0000040942217 – 298p12Add BLAST82
ChainiPRO_0000040943299 – 526Capsid protein p27Add BLAST228
ChainiPRO_0000040944527 – 621Nucleocapsid protein p14Add BLAST95
ChainiPRO_0000040945622 – 657p4Add BLAST36

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostBy similarity1

Post-translational modificationi

p10 is myristoylated.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Miscellaneous databases

PMAP-CutDBP07567.

Interactioni

Protein-protein interaction databases

DIPiDIP-45130N.
MINTiMINT-188975.

Structurei

Secondary structure

1657
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 20Combined sources16
Turni21 – 23Combined sources3
Helixi28 – 41Combined sources14
Turni46 – 48Combined sources3
Helixi54 – 68Combined sources15
Turni72 – 74Combined sources3
Helixi78 – 91Combined sources14
Helixi93 – 95Combined sources3
Helixi98 – 101Combined sources4
Helixi103 – 106Combined sources4
Beta strandi112 – 115Combined sources4
Beta strandi301 – 305Combined sources5
Beta strandi311 – 315Combined sources5
Helixi320 – 332Combined sources13
Helixi338 – 348Combined sources11
Helixi354 – 364Combined sources11
Helixi368 – 391Combined sources24
Beta strandi396 – 398Combined sources3
Turni399 – 401Combined sources3
Beta strandi404 – 406Combined sources3
Helixi408 – 414Combined sources7
Helixi417 – 430Combined sources14
Beta strandi596 – 599Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BAXNMR-A1-94[»]
1CL4NMR-A546-605[»]
2KGFNMR-A300-439[»]
2LPYNMR-A2-118[»]
2MV4NMR-A2-118[»]
4ARDelectron microscopy7.00A/B318-400[»]
5LDLNMR-A2-118[»]
5LMYNMR-A2-118[»]
ProteinModelPortaliP07567.
SMRiP07567.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07567.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi202 – 205PPXY motif4
Motifi210 – 213PTAP/PSAP motif4

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Core phosphoprotein pp24 contains two L domains: a PTAP/PSAP motif which interacts with the UEV domain of TSG101, and a PPXY motif which probably binds to the WW domains of HECT (homologous to E6-AP C-terminus) E3 ubiquitin ligases (By similarity).By similarity

Sequence similaritiesi

Contains 2 CCHC-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri547 – 564CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri576 – 593CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Family and domain databases

Gene3Di1.10.1200.30. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR003322. B_retro_matrix.
IPR000721. Gag_p24.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF02337. Gag_p10. 1 hit.
PF00607. Gag_p24. 1 hit.
[Graphical view]
ProDomiPD004265. B_retro_matrix_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 2 hits.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQELSQHER YVEQLKQALK TRGVKVKYAD LLKFFDFVKD TCPWFPQEGT
60 70 80 90 100
IDIKRWRRVG DCFQDYYNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM
110 120 130 140 150
AAVAQTEEIL KSNSQTDLTK TSQNPDLDLI SLDSDDEGAK SSSLQDKGLS
160 170 180 190 200
STKKPKRFPV LLTAQTSKDP EDPNPSEVDW DGLEDEAAKY HNPDWPPFLT
210 220 230 240 250
RPPPYNKATP SAPTVMAVVN PKEELKEKIA QLEEQIKLEE LHQALISKLQ
260 270 280 290 300
KLKTGNETVT HPDTAGGLSR TPHWPGQHIP KGKCCASREK EEQIPKDIFP
310 320 330 340 350
VTETVDGQGQ AWRHHNGFDF AVIKELKTAA SQYGATAPYT LAIVESVADN
360 370 380 390 400
WLTPTDWNTL VRAVLSGGDH LLWKSEFFEN CRDTAKRNQQ AGNGWDFDML
410 420 430 440 450
TGSGNYSSTD AQMQYDPGLF AQIQAAATKA WRKLPVKGDP GASLTGVKQG
460 470 480 490 500
PDEPFADFVH RLITTAGRIF GSAEAGVDYV KQLAYENANP ACQAAIRPYR
510 520 530 540 550
KKTDLTGYIR LCSDIGPSYQ QGLAMAAAFS GQTVKDFLNN KNKEKGGCCF
560 570 580 590 600
KCGKKGHFAK NCHEHAHNNA EPKVPGLCPR CKRGKHWANE CKSKTDNQGN
610 620 630 640 650
PIPPHQGNGW RGQPQAPKQA YGAVSFVPAN KNNPFQSLPE PPQEVQDWTS

VPPPTQY
Length:657
Mass (Da):73,110
Last modified:January 23, 2007 - v3
Checksum:i301888B033BC3061
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12349 Genomic RNA. Translation: AAA47710.1.
PIRiA25839. FOLJMP.
RefSeqiNP_056893.1. NC_001550.1.

Genome annotation databases

GeneIDi2746974.
KEGGivg:2746974.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12349 Genomic RNA. Translation: AAA47710.1.
PIRiA25839. FOLJMP.
RefSeqiNP_056893.1. NC_001550.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BAXNMR-A1-94[»]
1CL4NMR-A546-605[»]
2KGFNMR-A300-439[»]
2LPYNMR-A2-118[»]
2MV4NMR-A2-118[»]
4ARDelectron microscopy7.00A/B318-400[»]
5LDLNMR-A2-118[»]
5LMYNMR-A2-118[»]
ProteinModelPortaliP07567.
SMRiP07567.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-45130N.
MINTiMINT-188975.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2746974.
KEGGivg:2746974.

Miscellaneous databases

EvolutionaryTraceiP07567.
PMAP-CutDBP07567.

Family and domain databases

Gene3Di1.10.1200.30. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR003322. B_retro_matrix.
IPR000721. Gag_p24.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF02337. Gag_p10. 1 hit.
PF00607. Gag_p24. 1 hit.
[Graphical view]
ProDomiPD004265. B_retro_matrix_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 2 hits.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAG_MPMV
AccessioniPrimary (citable) accession number: P07567
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.