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P07560

- SEC4_YEAST

UniProt

P07560 - SEC4_YEAST

Protein

Ras-related protein SEC4

Gene

SEC4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 167 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Involved in exocytosis. Maybe by regulating the binding and fusion of secretory vesicles with the cell surface. The GTP-bound form of SEC4 may interact with an effector, thereby stimulating its activity and leading to exocytotic fusion. SEC4 may be an upstream activator of the 19.5S SEC8/SEC15 particle. SEC4 probably interacts directly with SEC8; it could serve as the attachment site for the SEC8/SEC15 particle.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi27 – 348GTP
    Nucleotide bindingi75 – 795GTP
    Nucleotide bindingi133 – 1364GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. ascospore-type prospore assembly Source: SGD
    2. autophagy Source: SGD
    3. bipolar cellular bud site selection Source: SGD
    4. exocytosis Source: SGD
    5. Golgi to plasma membrane transport Source: SGD
    6. GTP catabolic process Source: GOC
    7. membrane addition at site of cytokinesis Source: SGD
    8. protein transport Source: UniProtKB-KW
    9. small GTPase mediated signal transduction Source: SGD
    10. vesicle fusion Source: SGD

    Keywords - Biological processi

    Exocytosis, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30450-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein SEC4
    Alternative name(s):
    Suppressor of RHO3 protein 6
    Gene namesi
    Name:SEC4
    Synonyms:SRO6
    Ordered Locus Names:YFL005W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFL005w.
    SGDiS000001889. SEC4.

    Subcellular locationi

    GO - Cellular componenti

    1. actin cap Source: SGD
    2. incipient cellular bud site Source: SGD
    3. plasma membrane Source: UniProtKB-SubCell
    4. transport vesicle Source: SGD
    5. transport vesicle membrane Source: UniProtKB-SubCell
    6. vesicle Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 215215Ras-related protein SEC4PRO_0000121330Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei201 – 2011Phosphoserine3 Publications
    Modified residuei204 – 2041Phosphoserine2 Publications
    Lipidationi214 – 2141S-geranylgeranyl cysteineBy similarity
    Lipidationi215 – 2151S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Phosphoprotein, Prenylation

    Proteomic databases

    MaxQBiP07560.
    PaxDbiP07560.
    PeptideAtlasiP07560.
    PRIDEiP07560.

    Expressioni

    Gene expression databases

    GenevestigatoriP07560.

    Interactioni

    Subunit structurei

    Interacts with the guanyl-nucleotide exchange factor SEC2. Interacts with SRO7, YIF1, YIP3, YIP4 and YIP5.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    YIF1P538452EBI-16858,EBI-28230
    YIP3P536332EBI-16858,EBI-25301
    YIP4P530932EBI-16858,EBI-24124

    Protein-protein interaction databases

    BioGridi31141. 108 interactions.
    DIPiDIP-2492N.
    IntActiP07560. 11 interactions.
    MINTiMINT-689902.
    STRINGi4932.YFL005W.

    Structurei

    Secondary structure

    1
    215
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi20 – 289
    Beta strandi29 – 313
    Helixi33 – 4210
    Beta strandi44 – 463
    Turni50 – 523
    Beta strandi57 – 6610
    Beta strandi68 – 747
    Helixi79 – 813
    Helixi83 – 864
    Helixi87 – 904
    Beta strandi93 – 1019
    Helixi105 – 1095
    Helixi111 – 12111
    Turni122 – 1243
    Beta strandi127 – 1337
    Beta strandi136 – 1383
    Helixi144 – 15411
    Beta strandi158 – 1603
    Turni163 – 1664
    Helixi169 – 18214
    Turni183 – 1853

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G16X-ray1.80A/B/C/D18-187[»]
    1G17X-ray2.00A/B18-187[»]
    2EQBX-ray2.70A19-187[»]
    2OCYX-ray3.30C18-187[»]
    3CPHX-ray2.90A1-213[»]
    ProteinModelPortaliP07560.
    SMRiP07560. Positions 19-187.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07560.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi49 – 579Effector regionCurated

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00740000115017.
    HOGENOMiHOG000233968.
    KOiK07901.
    OMAiKVQREQA.
    OrthoDBiEOG7BGHXR.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07560-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGLRTVSAS SGNGKSYDSI MKILLIGDSG VGKSCLLVRF VEDKFNPSFI    50
    TTIGIDFKIK TVDINGKKVK LQLWDTAGQE RFRTITTAYY RGAMGIILVY 100
    DVTDERTFTN IKQWFKTVNE HANDEAQLLL VGNKSDMETR VVTADQGEAL 150
    AKELGIPFIE SSAKNDDNVN EIFFTLAKLI QEKIDSNKLV GVGNGKEGNI 200
    SINSGSGNSS KSNCC 215
    Length:215
    Mass (Da):23,506
    Last modified:August 1, 1988 - v1
    Checksum:i7DEDF7DCC7533BA7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16507 Genomic DNA. Translation: AAA35032.1.
    D50617 Genomic DNA. Translation: BAA09233.1.
    AY692843 Genomic DNA. Translation: AAT92862.1.
    BK006940 Genomic DNA. Translation: DAA12434.1.
    PIRiA25959. TVBYQ4.
    RefSeqiNP_116650.1. NM_001179961.1.

    Genome annotation databases

    EnsemblFungiiYFL005W; YFL005W; YFL005W.
    GeneIDi850543.
    KEGGisce:YFL005W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16507 Genomic DNA. Translation: AAA35032.1 .
    D50617 Genomic DNA. Translation: BAA09233.1 .
    AY692843 Genomic DNA. Translation: AAT92862.1 .
    BK006940 Genomic DNA. Translation: DAA12434.1 .
    PIRi A25959. TVBYQ4.
    RefSeqi NP_116650.1. NM_001179961.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1G16 X-ray 1.80 A/B/C/D 18-187 [» ]
    1G17 X-ray 2.00 A/B 18-187 [» ]
    2EQB X-ray 2.70 A 19-187 [» ]
    2OCY X-ray 3.30 C 18-187 [» ]
    3CPH X-ray 2.90 A 1-213 [» ]
    ProteinModelPortali P07560.
    SMRi P07560. Positions 19-187.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31141. 108 interactions.
    DIPi DIP-2492N.
    IntActi P07560. 11 interactions.
    MINTi MINT-689902.
    STRINGi 4932.YFL005W.

    Proteomic databases

    MaxQBi P07560.
    PaxDbi P07560.
    PeptideAtlasi P07560.
    PRIDEi P07560.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFL005W ; YFL005W ; YFL005W .
    GeneIDi 850543.
    KEGGi sce:YFL005W.

    Organism-specific databases

    CYGDi YFL005w.
    SGDi S000001889. SEC4.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00740000115017.
    HOGENOMi HOG000233968.
    KOi K07901.
    OMAi KVQREQA.
    OrthoDBi EOG7BGHXR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30450-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P07560.
    NextBioi 966307.

    Gene expression databases

    Genevestigatori P07560.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A ras-like protein is required for a post-Golgi event in yeast secretion."
      Salminen A., Novick P.J.
      Cell 49:527-538(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Sec2p mediates nucleotide exchange on Sec4p and is involved in polarized delivery of post-Golgi vesicles."
      Walch-Solimena C., Collins R.N., Novick P.J.
      J. Cell Biol. 137:1495-1509(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SEC2.
    7. "Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
      Calero M., Collins R.N.
      Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIP3.
    8. "Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
      Calero M., Winand N.J., Collins R.N.
      FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
    9. "The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p."
      Grosshans B.L., Andreeva A., Gangar A., Niessen S., Yates J.R. III, Brennwald P., Novick P.
      J. Cell Biol. 172:55-66(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SRO7.
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-201 AND SER-204, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-201, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-201 AND SER-204, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Crystal structures of a Rab protein in its inactive and active conformations."
      Stroupe C., Brunger A.T.
      J. Mol. Biol. 304:585-598(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 18-187 IN COMPLEX WITH GDP AND GTP ANALOG.

    Entry informationi

    Entry nameiSEC4_YEAST
    AccessioniPrimary (citable) accession number: P07560
    Secondary accession number(s): D6VTM4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 167 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3