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P07560

- SEC4_YEAST

UniProt

P07560 - SEC4_YEAST

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Protein
Ras-related protein SEC4
Gene
SEC4, SRO6, YFL005W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in exocytosis. Maybe by regulating the binding and fusion of secretory vesicles with the cell surface. The GTP-bound form of SEC4 may interact with an effector, thereby stimulating its activity and leading to exocytotic fusion. SEC4 may be an upstream activator of the 19.5S SEC8/SEC15 particle. SEC4 probably interacts directly with SEC8; it could serve as the attachment site for the SEC8/SEC15 particle.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 348GTP
Nucleotide bindingi75 – 795GTP
Nucleotide bindingi133 – 1364GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: SGD
  2. GTPase activity Source: SGD
  3. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. Golgi to plasma membrane transport Source: SGD
  3. ascospore-type prospore assembly Source: SGD
  4. autophagy Source: SGD
  5. bipolar cellular bud site selection Source: SGD
  6. exocytosis Source: SGD
  7. membrane addition at site of cytokinesis Source: SGD
  8. protein transport Source: UniProtKB-KW
  9. small GTPase mediated signal transduction Source: SGD
  10. vesicle fusion Source: SGD
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30450-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein SEC4
Alternative name(s):
Suppressor of RHO3 protein 6
Gene namesi
Name:SEC4
Synonyms:SRO6
Ordered Locus Names:YFL005W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFL005w.
SGDiS000001889. SEC4.

Subcellular locationi

GO - Cellular componenti

  1. actin cap Source: SGD
  2. incipient cellular bud site Source: SGD
  3. plasma membrane Source: UniProtKB-SubCell
  4. transport vesicle Source: SGD
  5. transport vesicle membrane Source: UniProtKB-SubCell
  6. vesicle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215Ras-related protein SEC4
PRO_0000121330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei201 – 2011Phosphoserine3 Publications
Modified residuei204 – 2041Phosphoserine2 Publications
Lipidationi214 – 2141S-geranylgeranyl cysteine By similarity
Lipidationi215 – 2151S-geranylgeranyl cysteine By similarity

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiP07560.
PaxDbiP07560.
PeptideAtlasiP07560.
PRIDEiP07560.

Expressioni

Gene expression databases

GenevestigatoriP07560.

Interactioni

Subunit structurei

Interacts with the guanyl-nucleotide exchange factor SEC2. Interacts with SRO7, YIF1, YIP3, YIP4 and YIP5.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
YIF1P538452EBI-16858,EBI-28230
YIP3P536332EBI-16858,EBI-25301
YIP4P530932EBI-16858,EBI-24124

Protein-protein interaction databases

BioGridi31141. 108 interactions.
DIPiDIP-2492N.
IntActiP07560. 11 interactions.
MINTiMINT-689902.
STRINGi4932.YFL005W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi20 – 289
Beta strandi29 – 313
Helixi33 – 4210
Beta strandi44 – 463
Turni50 – 523
Beta strandi57 – 6610
Beta strandi68 – 747
Helixi79 – 813
Helixi83 – 864
Helixi87 – 904
Beta strandi93 – 1019
Helixi105 – 1095
Helixi111 – 12111
Turni122 – 1243
Beta strandi127 – 1337
Beta strandi136 – 1383
Helixi144 – 15411
Beta strandi158 – 1603
Turni163 – 1664
Helixi169 – 18214
Turni183 – 1853

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G16X-ray1.80A/B/C/D18-187[»]
1G17X-ray2.00A/B18-187[»]
2EQBX-ray2.70A19-187[»]
2OCYX-ray3.30C18-187[»]
3CPHX-ray2.90A1-213[»]
ProteinModelPortaliP07560.
SMRiP07560. Positions 19-187.

Miscellaneous databases

EvolutionaryTraceiP07560.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi49 – 579Effector region Inferred

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00740000115017.
HOGENOMiHOG000233968.
KOiK07901.
OMAiKVQREQA.
OrthoDBiEOG7BGHXR.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07560-1 [UniParc]FASTAAdd to Basket

« Hide

MSGLRTVSAS SGNGKSYDSI MKILLIGDSG VGKSCLLVRF VEDKFNPSFI    50
TTIGIDFKIK TVDINGKKVK LQLWDTAGQE RFRTITTAYY RGAMGIILVY 100
DVTDERTFTN IKQWFKTVNE HANDEAQLLL VGNKSDMETR VVTADQGEAL 150
AKELGIPFIE SSAKNDDNVN EIFFTLAKLI QEKIDSNKLV GVGNGKEGNI 200
SINSGSGNSS KSNCC 215
Length:215
Mass (Da):23,506
Last modified:August 1, 1988 - v1
Checksum:i7DEDF7DCC7533BA7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M16507 Genomic DNA. Translation: AAA35032.1.
D50617 Genomic DNA. Translation: BAA09233.1.
AY692843 Genomic DNA. Translation: AAT92862.1.
BK006940 Genomic DNA. Translation: DAA12434.1.
PIRiA25959. TVBYQ4.
RefSeqiNP_116650.1. NM_001179961.1.

Genome annotation databases

EnsemblFungiiYFL005W; YFL005W; YFL005W.
GeneIDi850543.
KEGGisce:YFL005W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M16507 Genomic DNA. Translation: AAA35032.1 .
D50617 Genomic DNA. Translation: BAA09233.1 .
AY692843 Genomic DNA. Translation: AAT92862.1 .
BK006940 Genomic DNA. Translation: DAA12434.1 .
PIRi A25959. TVBYQ4.
RefSeqi NP_116650.1. NM_001179961.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G16 X-ray 1.80 A/B/C/D 18-187 [» ]
1G17 X-ray 2.00 A/B 18-187 [» ]
2EQB X-ray 2.70 A 19-187 [» ]
2OCY X-ray 3.30 C 18-187 [» ]
3CPH X-ray 2.90 A 1-213 [» ]
ProteinModelPortali P07560.
SMRi P07560. Positions 19-187.
ModBasei Search...

Protein-protein interaction databases

BioGridi 31141. 108 interactions.
DIPi DIP-2492N.
IntActi P07560. 11 interactions.
MINTi MINT-689902.
STRINGi 4932.YFL005W.

Proteomic databases

MaxQBi P07560.
PaxDbi P07560.
PeptideAtlasi P07560.
PRIDEi P07560.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFL005W ; YFL005W ; YFL005W .
GeneIDi 850543.
KEGGi sce:YFL005W.

Organism-specific databases

CYGDi YFL005w.
SGDi S000001889. SEC4.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00740000115017.
HOGENOMi HOG000233968.
KOi K07901.
OMAi KVQREQA.
OrthoDBi EOG7BGHXR.

Enzyme and pathway databases

BioCyci YEAST:G3O-30450-MONOMER.

Miscellaneous databases

EvolutionaryTracei P07560.
NextBioi 966307.

Gene expression databases

Genevestigatori P07560.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A ras-like protein is required for a post-Golgi event in yeast secretion."
    Salminen A., Novick P.J.
    Cell 49:527-538(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Sec2p mediates nucleotide exchange on Sec4p and is involved in polarized delivery of post-Golgi vesicles."
    Walch-Solimena C., Collins R.N., Novick P.J.
    J. Cell Biol. 137:1495-1509(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SEC2.
  7. "Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
    Calero M., Collins R.N.
    Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIP3.
  8. "Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
    Calero M., Winand N.J., Collins R.N.
    FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
  9. "The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p."
    Grosshans B.L., Andreeva A., Gangar A., Niessen S., Yates J.R. III, Brennwald P., Novick P.
    J. Cell Biol. 172:55-66(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SRO7.
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-201 AND SER-204, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-201, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-201 AND SER-204, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Crystal structures of a Rab protein in its inactive and active conformations."
    Stroupe C., Brunger A.T.
    J. Mol. Biol. 304:585-598(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 18-187 IN COMPLEX WITH GDP AND GTP ANALOG.

Entry informationi

Entry nameiSEC4_YEAST
AccessioniPrimary (citable) accession number: P07560
Secondary accession number(s): D6VTM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: July 9, 2014
This is version 166 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

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