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Protein

Ras-related protein SEC4

Gene

SEC4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in exocytosis. Maybe by regulating the binding and fusion of secretory vesicles with the cell surface. The GTP-bound form of SEC4 may interact with an effector, thereby stimulating its activity and leading to exocytotic fusion. SEC4 may be an upstream activator of the 19.5S SEC8/SEC15 particle. SEC4 probably interacts directly with SEC8; it could serve as the attachment site for the SEC8/SEC15 particle.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 34GTP8
Nucleotide bindingi75 – 79GTP5
Nucleotide bindingi133 – 136GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: SGD

GO - Biological processi

  • ascospore-type prospore assembly Source: SGD
  • autophagy Source: SGD
  • ER to Golgi vesicle-mediated transport Source: GO_Central
  • exocytosis Source: SGD
  • Golgi to plasma membrane transport Source: SGD
  • membrane addition at site of cytokinesis Source: SGD
  • protein transport Source: UniProtKB-KW
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle fusion Source: SGD
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30450-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein SEC4
Alternative name(s):
Suppressor of RHO3 protein 6
Gene namesi
Name:SEC4
Synonyms:SRO6
Ordered Locus Names:YFL005W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL005W.
SGDiS000001889. SEC4.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: GO_Central
  • Golgi membrane Source: GO_Central
  • incipient cellular bud site Source: SGD
  • mating projection tip Source: SGD
  • plasma membrane Source: UniProtKB-SubCell
  • transport vesicle Source: SGD
  • transport vesicle membrane Source: UniProtKB-SubCell
  • vesicle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001213301 – 215Ras-related protein SEC4Add BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201PhosphoserineCombined sources1
Modified residuei204PhosphoserineCombined sources1
Lipidationi214S-geranylgeranyl cysteineBy similarity1
Lipidationi215S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiP07560.
PRIDEiP07560.
TopDownProteomicsiP07560.

PTM databases

iPTMnetiP07560.

Interactioni

Subunit structurei

Interacts with the guanyl-nucleotide exchange factor SEC2. Interacts with SRO7, YIF1, YIP3, YIP4 and YIP5.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
YIF1P538452EBI-16858,EBI-28230
YIP3P536332EBI-16858,EBI-25301
YIP4P530932EBI-16858,EBI-24124

Protein-protein interaction databases

BioGridi31141. 131 interactors.
DIPiDIP-2492N.
IntActiP07560. 11 interactors.
MINTiMINT-689902.

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi20 – 28Combined sources9
Beta strandi29 – 31Combined sources3
Helixi33 – 42Combined sources10
Beta strandi44 – 46Combined sources3
Helixi49 – 52Combined sources4
Beta strandi57 – 66Combined sources10
Beta strandi68 – 74Combined sources7
Helixi79 – 81Combined sources3
Helixi83 – 85Combined sources3
Helixi87 – 90Combined sources4
Beta strandi93 – 101Combined sources9
Helixi105 – 109Combined sources5
Helixi111 – 121Combined sources11
Turni122 – 124Combined sources3
Beta strandi127 – 133Combined sources7
Beta strandi136 – 138Combined sources3
Helixi144 – 154Combined sources11
Beta strandi158 – 160Combined sources3
Turni163 – 166Combined sources4
Helixi169 – 182Combined sources14
Turni183 – 185Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G16X-ray1.80A/B/C/D18-187[»]
1G17X-ray2.00A/B18-187[»]
2EQBX-ray2.70A19-187[»]
2OCYX-ray3.30C18-187[»]
3CPHX-ray2.90A1-213[»]
4Z8YX-ray1.90A/B19-187[»]
4ZDWX-ray2.90A19-187[»]
ProteinModelPortaliP07560.
SMRiP07560.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07560.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi49 – 57Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

GeneTreeiENSGT00860000133675.
HOGENOMiHOG000233968.
InParanoidiP07560.
KOiK07901.
OMAiFENVENW.
OrthoDBiEOG092C5MDH.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLRTVSAS SGNGKSYDSI MKILLIGDSG VGKSCLLVRF VEDKFNPSFI
60 70 80 90 100
TTIGIDFKIK TVDINGKKVK LQLWDTAGQE RFRTITTAYY RGAMGIILVY
110 120 130 140 150
DVTDERTFTN IKQWFKTVNE HANDEAQLLL VGNKSDMETR VVTADQGEAL
160 170 180 190 200
AKELGIPFIE SSAKNDDNVN EIFFTLAKLI QEKIDSNKLV GVGNGKEGNI
210
SINSGSGNSS KSNCC
Length:215
Mass (Da):23,506
Last modified:August 1, 1988 - v1
Checksum:i7DEDF7DCC7533BA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16507 Genomic DNA. Translation: AAA35032.1.
D50617 Genomic DNA. Translation: BAA09233.1.
AY692843 Genomic DNA. Translation: AAT92862.1.
BK006940 Genomic DNA. Translation: DAA12434.1.
PIRiA25959. TVBYQ4.
RefSeqiNP_116650.1. NM_001179961.1.

Genome annotation databases

EnsemblFungiiBAA09233; BAA09233; BAA09233.
YFL005W; YFL005W; YFL005W.
GeneIDi850543.
KEGGisce:YFL005W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16507 Genomic DNA. Translation: AAA35032.1.
D50617 Genomic DNA. Translation: BAA09233.1.
AY692843 Genomic DNA. Translation: AAT92862.1.
BK006940 Genomic DNA. Translation: DAA12434.1.
PIRiA25959. TVBYQ4.
RefSeqiNP_116650.1. NM_001179961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G16X-ray1.80A/B/C/D18-187[»]
1G17X-ray2.00A/B18-187[»]
2EQBX-ray2.70A19-187[»]
2OCYX-ray3.30C18-187[»]
3CPHX-ray2.90A1-213[»]
4Z8YX-ray1.90A/B19-187[»]
4ZDWX-ray2.90A19-187[»]
ProteinModelPortaliP07560.
SMRiP07560.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31141. 131 interactors.
DIPiDIP-2492N.
IntActiP07560. 11 interactors.
MINTiMINT-689902.

PTM databases

iPTMnetiP07560.

Proteomic databases

MaxQBiP07560.
PRIDEiP07560.
TopDownProteomicsiP07560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09233; BAA09233; BAA09233.
YFL005W; YFL005W; YFL005W.
GeneIDi850543.
KEGGisce:YFL005W.

Organism-specific databases

EuPathDBiFungiDB:YFL005W.
SGDiS000001889. SEC4.

Phylogenomic databases

GeneTreeiENSGT00860000133675.
HOGENOMiHOG000233968.
InParanoidiP07560.
KOiK07901.
OMAiFENVENW.
OrthoDBiEOG092C5MDH.

Enzyme and pathway databases

BioCyciYEAST:G3O-30450-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiP07560.
PROiP07560.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSEC4_YEAST
AccessioniPrimary (citable) accession number: P07560
Secondary accession number(s): D6VTM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 30, 2016
This is version 191 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.