P07548 (DFD_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeotic protein deformed

Gene names

Name:	Dfd
ORF Names:	CG2189

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Homeotic protein controlling Drosophila head development. Transcriptional activator of the apoptotic activator protein rpr in cells at the maxillary/mandibular boundary. Ref.1 Ref.7
Subcellular location	Nucleus.
Tissue specificity	Expressed in the mandibular and maxillary segments of the head, with some expression in the posterior intercalary segment which become part of the CNS. In the imaginal tissues, expressed in part of the eye antennal disk which give rise to portions of the head capsule and to a small patch of expression in the basal-most portion of the labial disk. Ref.8
Sequence similarities	Belongs to the Antp homeobox family. Deformed subfamily. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	brain development Inferred from mutant phenotype. Source: FlyBase embryonic morphogenesis Inferred from mutant phenotype Ref.7. Source: FlyBase induction of apoptosis Inferred from mutant phenotype Ref.7. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from direct assay. Source: FlyBase sequence-specific DNA binding transcription factor activity Traceable author statement. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
exd	P40427	2	EBI-458925,EBI-101537

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	Homeotic protein deformed		PRO_0000200261
Regions
	DNA binding	366 – 425	60	Homeobox
	Motif	343 – 348	6	Antp-type hexapeptide
	Compositional bias	50 – 75	26	Gly-rich
	Compositional bias	76 – 158	83	His-rich
	Compositional bias	188 – 226	39	Gly-rich
	Compositional bias	290 – 331	42	Asp-rich
	Compositional bias	460 – 476	17	Gln-rich
	Compositional bias	524 – 551	28	Asn-rich
Experimental info
	Sequence conflict	32	1	L → V in CAA28782. Ref.1
	Sequence conflict	227	1	S → I in AAA28375. Ref.5
	Sequence conflict	442	1	G → A Ref.6
	Sequence conflict	443	1	N → K in AAA28375. Ref.5
	Sequence conflict	456	1	Missing Ref.6
	Sequence conflict	477	1	T → TQQT Ref.1
	Sequence conflict	477	1	T → TQQT Ref.5
	Sequence conflict	541	1	N → NN in CAA28782. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07548 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 9A7C79C744FAFF18	FASTA	586	63,380
	10 20 30 40 50 60 MSSFLMGYPH APHHVQSPMS MGNGLDPKFP PLADDYHHYN GHYSMTASTG HMSGAVGGGA 70 80 90 100 110 120 GVGSVGGGGA GGMTGHPHSM HPADMVSDYM AHHHNPHSHS HSHTHSLPHH HSNSAISGHH 130 140 150 160 170 180 QASAGGYSSN YANATPPSHP HSHPHAHPHQ SLGYYVHHAP EFISAGAVHS DPTNGYGPAA 190 200 210 220 230 240 NVPNTSNGGG GGGSGAVLGG GAVGGSANGY YGGYGGGYGT ANGSVGSTHS QGHSPHSQMM 250 260 270 280 290 300 DLPLQCSSTE PPTNTALGLQ ELGLKLEKRI EEAVPAGQQL QELGMRLRCD DMGSENDDMS 310 320 330 340 350 360 EEDRLMLDRS PDELGSNDND DDLGDSDSDE DLMAETTDGE RIIYPWMKKI HVAGVANGSY 370 380 390 400 410 420 QPGMEPKRQR TAYTRHQILE LEKEFHYNRY LTRRRRIEIA HTLVLSERQI KIWFQNRRMK 430 440 450 460 470 480 WKKDNKLPNT KNVRKKTVDA NGNPTPVAKK PTKRAASKKQ QQAQQQQQSQ QQQTQQTPVM 490 500 510 520 530 540 NECIRSDSLE SIGDVSSSLG NPPYIPAAPE TTSSYPGSQQ HLSNNNNNGS GNNNNNNNNN 550 560 570 580 NSNLNNNNNN NQMGHTNLHG HLQQQQSDLM TNLQLHIKQD YDLTAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Developmental and molecular analysis of Deformed: a homeotic gene controlling Drosophila head development." Regulski M., McGinnis N., Chadwick R., McGinnis W. EMBO J. 6:767-777(1987) [PubMed: 16453752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"Complete sequence of the Antennapedia complex of Drosophila." Celniker S.E., Pfeiffer B.D., Knafels J., Martin C.H., Mayeda C.A., Palazzolo M.J. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Berkeley.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"Common properties of proteins encoded by the Antennapedia complex genes of Drosophila melanogaster." Laughon A., Carroll S.B., Storfer F.A., Riley P.D., Scott M.P. Cold Spring Harb. Symp. Quant. Biol. 50:253-262(1985) [PubMed: 3938363] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 163-586.
[6]	"Homeo box genes of the Antennapedia and bithorax complexes of Drosophila." Regulski M., Harding K., Kostriken R., Karch F., Levine M., McGinnis W. Cell 43:71-80(1985) [PubMed: 2416463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 366-482.
[7]	"The Drosophila Hox gene deformed sculpts head morphology via direct regulation of the apoptosis activator reaper." Lohmann I., McGinnis N., Bodmer M., McGinnis W. Cell 110:457-466(2002) [PubMed: 12202035] [Abstract] Cited for: FUNCTION.
[8]	"Hox genes and the evolution of the arthropod body plan." Hughes C.L., Kaufman T.C. Evol. Dev. 4:459-499(2002) [PubMed: 12492146] [Abstract] Cited for: REVIEW ON FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X05136 Genomic DNA. Translation: CAA28782.1. AE001572 Genomic DNA. Translation: AAD19796.1. AE014297 Genomic DNA. Translation: AAF54083.2. M13373 mRNA. Translation: AAA28375.1.
PIR	A26638.
RefSeq	NP_477201.1. NM_057853.2.
UniGene	Dm.5282.
3D structure databases
ProteinModelPortal	P07548.
SMR	P07548. Positions 373-426.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-18055N.
IntAct	P07548. 20 interactions.
MINT	MINT-858733.
STRING	P07548.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0081621; FBpp0081138; FBgn0000439.
GeneID	40832.
KEGG	dme:Dmel_CG2189.
UCSC	CG2189-RA. d. melanogaster.
Organism-specific databases
CTD	40832.
FlyBase	FBgn0000439. Dfd.
Phylogenomic databases
eggNOG	inNOG04519.
GeneTree	EMGT00050000001991.
InParanoid	P07548.
OMA	QCSSTEP.
OrthoDB	EOG49CNQM.
PhylomeDB	P07548.
Gene expression databases
Bgee	P07548.
GermOnline	CG2189. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017995. Homeobox_antennapedia. IPR001827. Homeobox_Antennapedia_CS. IPR017970. Homeobox_CS. IPR020479. Homeobox_eu. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
KO	K09304.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00025. ANTENNAPEDIA. PR00024. HOMEOBOX.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00032. ANTENNAPEDIA. 1 hit. PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	820832.

Entry information

Entry name

DFD_DROME

Accession

Primary (citable) accession number: P07548
Secondary accession number(s): Q9UAL9, Q9VI48

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project