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Protein

Endolysin

Gene

15

Organism
Bacillus phage PZA (Bacteriophage PZA)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.By similarity

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei15 – 151Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Keywords - Biological processi

Cytolysis, Host cell lysis by virus, Virus exit from host cell

Protein family/group databases

CAZyiCBM50. Carbohydrate-Binding Module Family 50.
GH24. Glycoside Hydrolase Family 24.

Names & Taxonomyi

Protein namesi
Recommended name:
Endolysin (EC:3.2.1.17)
Alternative name(s):
Gene product 15
Short name:
gp15
Lysozyme
Morphogenesis protein 2
Muramidase
Protein p15
Gene namesi
Name:15
OrganismiBacillus phage PZA (Bacteriophage PZA)
Taxonomic identifieri10757 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaePicovirinaePhi29likevirus
Virus hostiBacillus subtilis [TaxID: 1423]
Proteomesi
  • UP000000855 Componenti: Genome

Subcellular locationi

  • Host cytoplasm By similarity

  • Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258EndolysinPRO_0000218106Add
BLAST

Expressioni

Keywords - Developmental stagei

Late protein

Structurei

3D structure databases

ProteinModelPortaliP07540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini161 – 20646LysM 1PROSITE-ProRule annotationAdd
BLAST
Domaini212 – 25746LysM 2PROSITE-ProRule annotationAdd
BLAST

Domaini

LysM domains are thought to be involved in peptidoglycan binding.By similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.Curated
Contains 2 LysM domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.10.530.40. 1 hit.
3.10.350.10. 2 hits.
InterProiIPR002196. Glyco_hydro_24.
IPR018392. LysM_dom.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view]
PfamiPF01476. LysM. 2 hits.
PF00959. Phage_lysozyme. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 2 hits.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF54106. SSF54106. 2 hits.
PROSITEiPS51782. LYSM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07540-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQISQAGINL IKSFEGLQLK AYKAVPTEKH YTIGYGHYGS DVSPRQVITA
60 70 80 90 100
KQAEDMLRDD VQAFVDGVNK ALKVSVTQNQ FDALVSFAYN VGLGAFRSSS
110 120 130 140 150
LLEYLNEGRT ALAAAEFPRW NKSGGKVYQG LVNRRAQEQA LFNSGTPKNV
160 170 180 190 200
SRGTSSSKVT PKYKVKSGDN LTKIAKKHNT TVATLLKLNP SIKDPNMIRV
210 220 230 240 250
GQTINVTGSG GKTHKVKSGD TLSKIAVDNK TTVSRLMSLN PEITNPNHIK

VGQTIRLS
Length:258
Mass (Da):28,052
Last modified:April 1, 1988 - v1
Checksum:i1A57FC5334637169
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11813 Genomic DNA. Translation: AAA88492.1.
PIRiA26215. WMBP15.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11813 Genomic DNA. Translation: AAA88492.1.
PIRiA26215. WMBP15.

3D structure databases

ProteinModelPortaliP07540.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM50. Carbohydrate-Binding Module Family 50.
GH24. Glycoside Hydrolase Family 24.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.530.40. 1 hit.
3.10.350.10. 2 hits.
InterProiIPR002196. Glyco_hydro_24.
IPR018392. LysM_dom.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view]
PfamiPF01476. LysM. 2 hits.
PF00959. Phage_lysozyme. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 2 hits.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF54106. SSF54106. 2 hits.
PROSITEiPS51782. LYSM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENLYS_BPPZA
AccessioniPrimary (citable) accession number: P07540
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: July 6, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.