P07529 (XYNA_CRYAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endo-1,4-beta-xylanase Short name=Xylanase EC=3.2.1.8 Alternative name(s): 1,4-beta-D-xylan xylanohydrolase
Organism	Cryptococcus albidus (Filobasidium floriforme)
Taxonomic identifier	100951 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Filobasidiales › mitosporic Filobasidiales › Cryptococcus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Requires at least three xylose residues for catalytic activity. Does not have activity against xylobiose.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 10 (cellulase F) family.

Ontologies

Keywords
Biological process	Xylan degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	endo-1,4-beta-xylanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.2
	Chain	22 – 332	311	Endo-1,4-beta-xylanase		PRO_0000007971
Sites
	Active site	120	1	Proton donor By similarity
	Active site	214	1	Nucleophile By similarity
Amino acid modifications
	Disulfide bond	128 ↔ 160		By similarity
	Disulfide bond	247 ↔ 253		By similarity
Experimental info
	Sequence conflict	88 – 90	3	TGT → AGL AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P07529 [UniParc]. Last modified July 1, 1989. Version 2. Checksum: 1814FD823E48306A	FASTA	332	35,776
	10 20 30 40 50 60 MLSSTTLLAI LSALALTSVQ AAPADKNSLD YLANKAGKRY LGTAVQSPQL VPGSQYVQIL 70 80 90 100 110 120 ESQFDAITPE NEMKWEVVEP TEGNFDFTGT DKIVAEAKKT GSLLRGHNIC WDSQLRYAHE 130 140 150 160 170 180 VAPKMKLCIN DYNIETVNAK SQAMAKVAAG LLAKGAPLHC IGMFKNAKRR SSGLLIRTAS 190 200 210 220 230 240 SGLESHFIGG STPKDIPAAM NLFSDQGLEV PMTELDVRIP VNGNDMPANA TVAKEQVDDY 250 260 270 280 290 300 YTSVSACLGN DLCPGVSIWQ FADPTSWIPG VFKGKLIAVS CTFSGCLLQY CVGYGAALLY 310 320 330 DAQYQPKSTY YVVQQALKDG KNSGSKFHGI KL

References

[1]	"Complete nucleotide sequence of the xylanase gene from the yeast Cryptococcus albidus." Boucher F., Morosoli R., Durand S. Nucleic Acids Res. 16:9874-9874(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CCY 17-4-4.
[2]	"Isolation and partial primary sequence of a xylanase from the yeast Cryptococcus albidus." Morosoli R., Roy C., Yaguchi M. Biochim. Biophys. Acta 870:473-478(1986) Cited for: PROTEIN SEQUENCE OF 22-93.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X12596 Genomic DNA. Translation: CAA31109.1.
PIR	JS0734.
3D structure databases
ProteinModelPortal	P07529.
ModBase	Search...
Protein family/group databases
CAZy	GH10. Glycoside Hydrolase Family 10.
mycoCLAP	XYN10A_CRYAL.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 2 hits.
InterPro	IPR001000. Glyco_hydro_10. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00331. Glyco_hydro_10. 2 hits. [Graphical view]
SMART	SM00633. Glyco_10. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00591. GLYCOSYL_HYDROL_F10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYNA_CRYAL

Accession

Primary (citable) accession number: P07529

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program