P07527 (WEE1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitosis inhibitor protein kinase wee1
EC=2.7.10.2

Alternative name(s):
P107 protein kinase homolog

Gene names

Name:	wee1
ORF Names:	SPCC18B5.03

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	877 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein kinase that acts both on serines and on tyrosines. It acts as a dosage-dependent negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2. Ref.4
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Enzyme regulation	Negatively regulated by phosphorylation in the M-phase.
Subcellular location	Nucleus Ref.3.
Post-translational modification	Phosphorylated in the C-terminal by NIM1/CDR1.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage checkpoint Traceable author statement PubMed 1549179. Source: PomBase activation of bipolar cell growth Inferred from mutant phenotype PubMed 20501954. Source: PomBase cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of G2/M transition of mitotic cell cycle Inferred from mutant phenotype PubMed 22624651Ref.1. Source: PomBase negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M Inferred from direct assay PubMed 8428596. Source: PomBase negative regulation of protein kinase activity by regulation of protein phosphorylation Inferred from direct assay PubMed 1372994. Source: PomBase peptidyl-serine autophosphorylation Inferred from direct assay Ref.4. Source: PomBase peptidyl-tyrosine autophosphorylation Inferred from direct assay Ref.4. Source: PomBase regulation of cell size Non-traceable author statement. Source: PomBase
Cellular_component	medial cortical node Inferred from direct assay PubMed 19474792. Source: PomBase mitotic spindle pole body Inferred from direct assay PubMed 16823372. Source: PomBase nucleoplasm Traceable author statement PubMed 9034337. Source: PomBase
Molecular_function	ATP binding Inferred from sequence model. Source: PomBase cyclin-dependent protein serine/threonine kinase activity Inferred from direct assay PubMed 1372994. Source: PomBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from direct assay Ref.4. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 877	877	Mitosis inhibitor protein kinase wee1		PRO_0000086815
Regions
	Domain	566 – 843	278	Protein kinase
	Nucleotide binding	572 – 580	9	ATP By similarity
Sites
	Active site	693	1	Proton acceptor By similarity
	Metal binding	698	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	711	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	596	1	ATP By similarity
Experimental info
	Mutagenesis	596	1	K → L: Inactivates enzyme.

Sequences

	Sequence		Length	Mass (Da)
	P07527 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: C8B5113D66C39D10	FASTA	877	96,261
	10 20 30 40 50 60 MSSSSNTSSH RSYGLRRSQR SMNLNRATLL APPTPSSLYD ANNSTSSTSS QKPNTSFTSL 70 80 90 100 110 120 FGPRKQTTSS PSFSHAAPLH PLSPPSFTHS QPQIQAQPVP RRPSLFDRPN LVSRSSSRLG 130 140 150 160 170 180 DSPSLSPVAQ VANPIHHTAP SPSDVRAFPI HKNASTGVKR SFFSSSMSNG AMSPPSHSPS 190 200 210 220 230 240 PFLQSSQHIP PSTPAQKLRK KNNFDSFRIS NSHISPFASG SFSPFATSSP NFLSTSTPAP 250 260 270 280 290 300 PNSNNANPST LFSSIPSSRH TTSNHFPSNS AQSSLFSPTA RPLTARKLGF ASSQTKSAVS 310 320 330 340 350 360 NNHSRNSSKD ASFMMKSFIP SNRSHPQTQQ NESSLFSDNS MVNSSSNSFS LFPNATLPNP 370 380 390 400 410 420 PSSELLTTPF QQIKPPSQVF MSTGLLSKQH RPRKNINFTP LPPSTPSKPS TFVRPHSSST 430 440 450 460 470 480 DSPPSPSTPS NTQTDSYFIQ RENTPTNHNS IPTIQLEKSS MDFLRFDPPP SAVKTSHNYG 490 500 510 520 530 540 LPFLSNQRCP ATPTRNPFAF ENTVSIHMDG RQPSPIKSRN NNQMSFAMEE EADVSQPSSS 550 560 570 580 590 600 SFTLSFPSAL TSSKVSSSTS HLLTRFRNVT LLGSGEFSEV FQVEDPVEKT LKYAVKKLKV 610 620 630 640 650 660 KFSGPKERNR LLQEVSIQRA LKGHDHIVEL MDSWEHGGFL YMQVELCENG SLDRFLEEQG 670 680 690 700 710 720 QLSRLDEFRV WKILVEVALG LQFIHHKNYV HLDLKPANVM ITFEGTLKIG DFGMASVWPV 730 740 750 760 770 780 PRGMEREGDC EYIAPEVLAN HLYDKPADIF SLGITVFEAA ANIVLPDNGQ SWQKLRSGDL 790 800 810 820 830 840 SDAPRLSSTD NGSSLTSSSR ETPANSIIGQ GGLDRVVEWM LSPEPRNRPT IDQILATDEV 850 860 870 CWVEMRRKAG AIIYEGIHGS SSNPQGDQMM EDWQVNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog." Russell P., Nurse P. Cell 49:559-567(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 406-625, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968.
[4]	"Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase." Featherstone C., Russell P. Nature 349:808-811(1991) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M16508 Genomic DNA. Translation: AAA35354.1. CU329672 Genomic DNA. Translation: CAB52150.1. AB027900 Genomic DNA. Translation: BAA87204.1.
PIR	A25962.
RefSeq	NP_587933.1. NM_001022924.2.
3D structure databases
ProteinModelPortal	P07527.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-16N.
STRING	4896.SPCC18B5.03-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC18B5.03.1; SPCC18B5.03.1:pep; SPCC18B5.03.
GeneID	2539123.
KEGG	spo:SPCC18B5.03.
Organism-specific databases
PomBase	SPCC18B5.03.
Phylogenomic databases
KO	K03114.
OMA	YELARCK.
OrthoDB	EOG486CN1.
Enzyme and pathway databases
Reactome	REACT_118136. Cell Cycle.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20800295.

Entry information

Entry name

WEE1_SCHPO

Accession

Primary (citable) accession number: P07527
Secondary accession number(s): Q9UU00

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 1, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program