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P07527

- WEE1_SCHPO

UniProt

P07527 - WEE1_SCHPO

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Protein

Mitosis inhibitor protein kinase wee1

Gene

wee1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Protein kinase that acts both on serines and on tyrosines. It acts as a dosage-dependent negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Enzyme regulationi

Negatively regulated by phosphorylation in the M-phase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei596 – 5961ATPPROSITE-ProRule annotation
Active sitei693 – 6931Proton acceptorPROSITE-ProRule annotation
Metal bindingi698 – 6981Magnesium; via carbonyl oxygenBy similarity
Metal bindingi711 – 7111Magnesium; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi572 – 5809ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: PomBase
  3. metal ion binding Source: UniProtKB-KW
  4. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  5. protein serine/threonine kinase activity Source: PomBase
  6. protein tyrosine kinase activity Source: PomBase

GO - Biological processi

  1. mitotic cell cycle checkpoint Source: PomBase
  2. mitotic DNA damage checkpoint Source: PomBase
  3. mitotic nuclear division Source: UniProtKB-KW
  4. negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
  5. negative regulation of protein kinase activity by regulation of protein phosphorylation Source: PomBase
  6. peptidyl-serine autophosphorylation Source: PomBase
  7. peptidyl-tyrosine autophosphorylation Source: PomBase
  8. regulation of cell size Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_217024. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_243134. Polo-like kinase mediated events.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitosis inhibitor protein kinase wee1 (EC:2.7.10.2)
Alternative name(s):
P107 protein kinase homolog
Gene namesi
Name:wee1
ORF Names:SPCC18B5.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome III

Organism-specific databases

PomBaseiSPCC18B5.03.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. medial cortical node Source: PomBase
  2. mitotic spindle pole body Source: PomBase
  3. nucleoplasm Source: PomBase
  4. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi596 – 5961K → L: Inactivates enzyme.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 877877Mitosis inhibitor protein kinase wee1PRO_0000086815Add
BLAST

Post-translational modificationi

Phosphorylated in the C-terminal by NIM1/CDR1.

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

BioGridi275695. 73 interactions.
DIPiDIP-16N.
MINTiMINT-4686928.
STRINGi4896.SPCC18B5.03-1.

Structurei

3D structure databases

ProteinModelPortaliP07527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini566 – 843278Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP07527.
KOiK03114.
OMAiYELARCK.
OrthoDBiEOG7380FJ.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07527-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSSSNTSSH RSYGLRRSQR SMNLNRATLL APPTPSSLYD ANNSTSSTSS
60 70 80 90 100
QKPNTSFTSL FGPRKQTTSS PSFSHAAPLH PLSPPSFTHS QPQIQAQPVP
110 120 130 140 150
RRPSLFDRPN LVSRSSSRLG DSPSLSPVAQ VANPIHHTAP SPSDVRAFPI
160 170 180 190 200
HKNASTGVKR SFFSSSMSNG AMSPPSHSPS PFLQSSQHIP PSTPAQKLRK
210 220 230 240 250
KNNFDSFRIS NSHISPFASG SFSPFATSSP NFLSTSTPAP PNSNNANPST
260 270 280 290 300
LFSSIPSSRH TTSNHFPSNS AQSSLFSPTA RPLTARKLGF ASSQTKSAVS
310 320 330 340 350
NNHSRNSSKD ASFMMKSFIP SNRSHPQTQQ NESSLFSDNS MVNSSSNSFS
360 370 380 390 400
LFPNATLPNP PSSELLTTPF QQIKPPSQVF MSTGLLSKQH RPRKNINFTP
410 420 430 440 450
LPPSTPSKPS TFVRPHSSST DSPPSPSTPS NTQTDSYFIQ RENTPTNHNS
460 470 480 490 500
IPTIQLEKSS MDFLRFDPPP SAVKTSHNYG LPFLSNQRCP ATPTRNPFAF
510 520 530 540 550
ENTVSIHMDG RQPSPIKSRN NNQMSFAMEE EADVSQPSSS SFTLSFPSAL
560 570 580 590 600
TSSKVSSSTS HLLTRFRNVT LLGSGEFSEV FQVEDPVEKT LKYAVKKLKV
610 620 630 640 650
KFSGPKERNR LLQEVSIQRA LKGHDHIVEL MDSWEHGGFL YMQVELCENG
660 670 680 690 700
SLDRFLEEQG QLSRLDEFRV WKILVEVALG LQFIHHKNYV HLDLKPANVM
710 720 730 740 750
ITFEGTLKIG DFGMASVWPV PRGMEREGDC EYIAPEVLAN HLYDKPADIF
760 770 780 790 800
SLGITVFEAA ANIVLPDNGQ SWQKLRSGDL SDAPRLSSTD NGSSLTSSSR
810 820 830 840 850
ETPANSIIGQ GGLDRVVEWM LSPEPRNRPT IDQILATDEV CWVEMRRKAG
860 870
AIIYEGIHGS SSNPQGDQMM EDWQVNV
Length:877
Mass (Da):96,261
Last modified:April 1, 1988 - v1
Checksum:iC8B5113D66C39D10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16508 Genomic DNA. Translation: AAA35354.1.
CU329672 Genomic DNA. Translation: CAB52150.1.
AB027900 Genomic DNA. Translation: BAA87204.1.
PIRiA25962.
RefSeqiNP_587933.1. NM_001022924.2.

Genome annotation databases

EnsemblFungiiSPCC18B5.03.1; SPCC18B5.03.1:pep; SPCC18B5.03.
GeneIDi2539123.
KEGGispo:SPCC18B5.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16508 Genomic DNA. Translation: AAA35354.1 .
CU329672 Genomic DNA. Translation: CAB52150.1 .
AB027900 Genomic DNA. Translation: BAA87204.1 .
PIRi A25962.
RefSeqi NP_587933.1. NM_001022924.2.

3D structure databases

ProteinModelPortali P07527.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 275695. 73 interactions.
DIPi DIP-16N.
MINTi MINT-4686928.
STRINGi 4896.SPCC18B5.03-1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPCC18B5.03.1 ; SPCC18B5.03.1:pep ; SPCC18B5.03 .
GeneIDi 2539123.
KEGGi spo:SPCC18B5.03.

Organism-specific databases

PomBasei SPCC18B5.03.

Phylogenomic databases

InParanoidi P07527.
KOi K03114.
OMAi YELARCK.
OrthoDBi EOG7380FJ.

Enzyme and pathway databases

Reactomei REACT_217024. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_243134. Polo-like kinase mediated events.

Miscellaneous databases

NextBioi 20800295.
PROi P07527.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog."
    Russell P., Nurse P.
    Cell 49:559-567(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
    Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
    Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 406-625, SUBCELLULAR LOCATION.
    Strain: ATCC 38364 / 968.
  4. "Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase."
    Featherstone C., Russell P.
    Nature 349:808-811(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiWEE1_SCHPO
AccessioniPrimary (citable) accession number: P07527
Secondary accession number(s): Q9UU00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 26, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3