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Protein

Mitosis inhibitor protein kinase wee1

Gene

wee1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase that acts both on serines and on tyrosines. It acts as a dosage-dependent negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Enzyme regulationi

Negatively regulated by phosphorylation in the M-phase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei596ATPPROSITE-ProRule annotation1
Active sitei693Proton acceptorPROSITE-ProRule annotation1
Metal bindingi698Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi711Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi572 – 580ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic cytokinesis checkpoint Source: PomBase
  • mitotic DNA damage checkpoint Source: PomBase
  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • negative regulation of meiotic nuclear division Source: PomBase
  • peptidyl-serine phosphorylation Source: PomBase
  • peptidyl-tyrosine phosphorylation Source: PomBase
  • signal transduction involved in G2 cell size control checkpoint Source: PomBase
  • signal transduction involved in mitotic DNA damage checkpoint Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-156711. Polo-like kinase mediated events.
R-SPO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitosis inhibitor protein kinase wee1 (EC:2.7.10.2)
Alternative name(s):
P107 protein kinase homolog
Gene namesi
Name:wee1
ORF Names:SPCC18B5.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC18B5.03.
PomBaseiSPCC18B5.03. wee1.

Subcellular locationi

GO - Cellular componenti

  • medial cortical node Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nucleoplasm Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi596K → L: Inactivates enzyme. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000868151 – 877Mitosis inhibitor protein kinase wee1Add BLAST877

Post-translational modificationi

Phosphorylated in the C-terminal by NIM1/CDR1.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP07527.

PTM databases

SwissPalmiP07527.

Interactioni

Protein-protein interaction databases

BioGridi275695. 75 interactors.
DIPiDIP-16N.
MINTiMINT-4686928.

Structurei

3D structure databases

ProteinModelPortaliP07527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini566 – 843Protein kinasePROSITE-ProRule annotationAdd BLAST278

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP07527.
KOiK03114.
OMAiYELARCK.
OrthoDBiEOG092C38EF.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSSNTSSH RSYGLRRSQR SMNLNRATLL APPTPSSLYD ANNSTSSTSS
60 70 80 90 100
QKPNTSFTSL FGPRKQTTSS PSFSHAAPLH PLSPPSFTHS QPQIQAQPVP
110 120 130 140 150
RRPSLFDRPN LVSRSSSRLG DSPSLSPVAQ VANPIHHTAP SPSDVRAFPI
160 170 180 190 200
HKNASTGVKR SFFSSSMSNG AMSPPSHSPS PFLQSSQHIP PSTPAQKLRK
210 220 230 240 250
KNNFDSFRIS NSHISPFASG SFSPFATSSP NFLSTSTPAP PNSNNANPST
260 270 280 290 300
LFSSIPSSRH TTSNHFPSNS AQSSLFSPTA RPLTARKLGF ASSQTKSAVS
310 320 330 340 350
NNHSRNSSKD ASFMMKSFIP SNRSHPQTQQ NESSLFSDNS MVNSSSNSFS
360 370 380 390 400
LFPNATLPNP PSSELLTTPF QQIKPPSQVF MSTGLLSKQH RPRKNINFTP
410 420 430 440 450
LPPSTPSKPS TFVRPHSSST DSPPSPSTPS NTQTDSYFIQ RENTPTNHNS
460 470 480 490 500
IPTIQLEKSS MDFLRFDPPP SAVKTSHNYG LPFLSNQRCP ATPTRNPFAF
510 520 530 540 550
ENTVSIHMDG RQPSPIKSRN NNQMSFAMEE EADVSQPSSS SFTLSFPSAL
560 570 580 590 600
TSSKVSSSTS HLLTRFRNVT LLGSGEFSEV FQVEDPVEKT LKYAVKKLKV
610 620 630 640 650
KFSGPKERNR LLQEVSIQRA LKGHDHIVEL MDSWEHGGFL YMQVELCENG
660 670 680 690 700
SLDRFLEEQG QLSRLDEFRV WKILVEVALG LQFIHHKNYV HLDLKPANVM
710 720 730 740 750
ITFEGTLKIG DFGMASVWPV PRGMEREGDC EYIAPEVLAN HLYDKPADIF
760 770 780 790 800
SLGITVFEAA ANIVLPDNGQ SWQKLRSGDL SDAPRLSSTD NGSSLTSSSR
810 820 830 840 850
ETPANSIIGQ GGLDRVVEWM LSPEPRNRPT IDQILATDEV CWVEMRRKAG
860 870
AIIYEGIHGS SSNPQGDQMM EDWQVNV
Length:877
Mass (Da):96,261
Last modified:April 1, 1988 - v1
Checksum:iC8B5113D66C39D10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16508 Genomic DNA. Translation: AAA35354.1.
CU329672 Genomic DNA. Translation: CAB52150.1.
AB027900 Genomic DNA. Translation: BAA87204.1.
PIRiA25962.
RefSeqiNP_587933.1. NM_001022924.2.

Genome annotation databases

EnsemblFungiiSPCC18B5.03.1; SPCC18B5.03.1:pep; SPCC18B5.03.
GeneIDi2539123.
KEGGispo:SPCC18B5.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16508 Genomic DNA. Translation: AAA35354.1.
CU329672 Genomic DNA. Translation: CAB52150.1.
AB027900 Genomic DNA. Translation: BAA87204.1.
PIRiA25962.
RefSeqiNP_587933.1. NM_001022924.2.

3D structure databases

ProteinModelPortaliP07527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275695. 75 interactors.
DIPiDIP-16N.
MINTiMINT-4686928.

PTM databases

SwissPalmiP07527.

Proteomic databases

PRIDEiP07527.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC18B5.03.1; SPCC18B5.03.1:pep; SPCC18B5.03.
GeneIDi2539123.
KEGGispo:SPCC18B5.03.

Organism-specific databases

EuPathDBiFungiDB:SPCC18B5.03.
PomBaseiSPCC18B5.03. wee1.

Phylogenomic databases

InParanoidiP07527.
KOiK03114.
OMAiYELARCK.
OrthoDBiEOG092C38EF.

Enzyme and pathway databases

ReactomeiR-SPO-156711. Polo-like kinase mediated events.
R-SPO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Miscellaneous databases

PROiP07527.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWEE1_SCHPO
AccessioniPrimary (citable) accession number: P07527
Secondary accession number(s): Q9UU00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 30, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.