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P07527

- WEE1_SCHPO

UniProt

P07527 - WEE1_SCHPO

Protein

Mitosis inhibitor protein kinase wee1

Gene

wee1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Protein kinase that acts both on serines and on tyrosines. It acts as a dosage-dependent negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2.1 Publication

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 2 magnesium ions per subunit.By similarity

    Enzyme regulationi

    Negatively regulated by phosphorylation in the M-phase.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei596 – 5961ATPPROSITE-ProRule annotation
    Active sitei693 – 6931Proton acceptorPROSITE-ProRule annotation
    Metal bindingi698 – 6981Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi711 – 7111Magnesium; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi572 – 5809ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: PomBase
    3. metal ion binding Source: UniProtKB-KW
    4. non-membrane spanning protein tyrosine kinase activity Source: PomBase
    5. protein binding Source: PomBase
    6. protein serine/threonine kinase activity Source: PomBase

    GO - Biological processi

    1. activation of bipolar cell growth Source: PomBase
    2. mitotic cell cycle checkpoint Source: PomBase
    3. mitotic DNA damage checkpoint Source: PomBase
    4. mitotic nuclear division Source: UniProtKB-KW
    5. negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle Source: PomBase
    6. negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
    7. negative regulation of protein kinase activity by regulation of protein phosphorylation Source: PomBase
    8. peptidyl-serine autophosphorylation Source: PomBase
    9. peptidyl-tyrosine autophosphorylation Source: PomBase
    10. peptidyl-tyrosine phosphorylation Source: PomBase
    11. regulation of cell size Source: PomBase

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_217024. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitosis inhibitor protein kinase wee1 (EC:2.7.10.2)
    Alternative name(s):
    P107 protein kinase homolog
    Gene namesi
    Name:wee1
    ORF Names:SPCC18B5.03
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome III

    Organism-specific databases

    PomBaseiSPCC18B5.03.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. medial cortical node Source: PomBase
    2. mitotic spindle pole body Source: PomBase
    3. nucleoplasm Source: PomBase
    4. nucleus Source: PomBase

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi596 – 5961K → L: Inactivates enzyme.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 877877Mitosis inhibitor protein kinase wee1PRO_0000086815Add
    BLAST

    Post-translational modificationi

    Phosphorylated in the C-terminal by NIM1/CDR1.

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    BioGridi275695. 70 interactions.
    DIPiDIP-16N.
    MINTiMINT-4686928.
    STRINGi4896.SPCC18B5.03-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP07527.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini566 – 843278Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    KOiK03114.
    OMAiYELARCK.
    OrthoDBiEOG7380FJ.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07527-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSSNTSSH RSYGLRRSQR SMNLNRATLL APPTPSSLYD ANNSTSSTSS    50
    QKPNTSFTSL FGPRKQTTSS PSFSHAAPLH PLSPPSFTHS QPQIQAQPVP 100
    RRPSLFDRPN LVSRSSSRLG DSPSLSPVAQ VANPIHHTAP SPSDVRAFPI 150
    HKNASTGVKR SFFSSSMSNG AMSPPSHSPS PFLQSSQHIP PSTPAQKLRK 200
    KNNFDSFRIS NSHISPFASG SFSPFATSSP NFLSTSTPAP PNSNNANPST 250
    LFSSIPSSRH TTSNHFPSNS AQSSLFSPTA RPLTARKLGF ASSQTKSAVS 300
    NNHSRNSSKD ASFMMKSFIP SNRSHPQTQQ NESSLFSDNS MVNSSSNSFS 350
    LFPNATLPNP PSSELLTTPF QQIKPPSQVF MSTGLLSKQH RPRKNINFTP 400
    LPPSTPSKPS TFVRPHSSST DSPPSPSTPS NTQTDSYFIQ RENTPTNHNS 450
    IPTIQLEKSS MDFLRFDPPP SAVKTSHNYG LPFLSNQRCP ATPTRNPFAF 500
    ENTVSIHMDG RQPSPIKSRN NNQMSFAMEE EADVSQPSSS SFTLSFPSAL 550
    TSSKVSSSTS HLLTRFRNVT LLGSGEFSEV FQVEDPVEKT LKYAVKKLKV 600
    KFSGPKERNR LLQEVSIQRA LKGHDHIVEL MDSWEHGGFL YMQVELCENG 650
    SLDRFLEEQG QLSRLDEFRV WKILVEVALG LQFIHHKNYV HLDLKPANVM 700
    ITFEGTLKIG DFGMASVWPV PRGMEREGDC EYIAPEVLAN HLYDKPADIF 750
    SLGITVFEAA ANIVLPDNGQ SWQKLRSGDL SDAPRLSSTD NGSSLTSSSR 800
    ETPANSIIGQ GGLDRVVEWM LSPEPRNRPT IDQILATDEV CWVEMRRKAG 850
    AIIYEGIHGS SSNPQGDQMM EDWQVNV 877
    Length:877
    Mass (Da):96,261
    Last modified:April 1, 1988 - v1
    Checksum:iC8B5113D66C39D10
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16508 Genomic DNA. Translation: AAA35354.1.
    CU329672 Genomic DNA. Translation: CAB52150.1.
    AB027900 Genomic DNA. Translation: BAA87204.1.
    PIRiA25962.
    RefSeqiNP_587933.1. NM_001022924.2.

    Genome annotation databases

    EnsemblFungiiSPCC18B5.03.1; SPCC18B5.03.1:pep; SPCC18B5.03.
    GeneIDi2539123.
    KEGGispo:SPCC18B5.03.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16508 Genomic DNA. Translation: AAA35354.1 .
    CU329672 Genomic DNA. Translation: CAB52150.1 .
    AB027900 Genomic DNA. Translation: BAA87204.1 .
    PIRi A25962.
    RefSeqi NP_587933.1. NM_001022924.2.

    3D structure databases

    ProteinModelPortali P07527.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 275695. 70 interactions.
    DIPi DIP-16N.
    MINTi MINT-4686928.
    STRINGi 4896.SPCC18B5.03-1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPCC18B5.03.1 ; SPCC18B5.03.1:pep ; SPCC18B5.03 .
    GeneIDi 2539123.
    KEGGi spo:SPCC18B5.03.

    Organism-specific databases

    PomBasei SPCC18B5.03.

    Phylogenomic databases

    KOi K03114.
    OMAi YELARCK.
    OrthoDBi EOG7380FJ.

    Enzyme and pathway databases

    Reactomei REACT_217024. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

    Miscellaneous databases

    NextBioi 20800295.
    PROi P07527.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog."
      Russell P., Nurse P.
      Cell 49:559-567(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
      Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
      Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 406-625, SUBCELLULAR LOCATION.
      Strain: ATCC 38364 / 968.
    4. "Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase."
      Featherstone C., Russell P.
      Nature 349:808-811(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiWEE1_SCHPO
    AccessioniPrimary (citable) accession number: P07527
    Secondary accession number(s): Q9UU00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3