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P07522

- EGF_RAT

UniProt

P07522 - EGF_RAT

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Protein
Pro-epidermal growth factor
Gene
Egf
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 By similarity.

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. epidermal growth factor receptor binding Source: RGD

GO - Biological processi

  1. STAT protein import into nucleus Source: UniProtKB
  2. epidermal growth factor receptor signaling pathway Source: RGD
  3. intracellular signal transduction Source: RGD
  4. positive regulation of DNA binding Source: UniProtKB
  5. regulation of protein secretion Source: RGD
  6. regulation of protein transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-epidermal growth factor
Short name:
EGF
Cleaved into the following chain:
Gene namesi
Name:Egf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2542. Egf.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 10351014Extracellular Reviewed prediction
Add
BLAST
Transmembranei1036 – 105722Helical; Reviewed prediction
Add
BLAST
Topological domaini1058 – 113376Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 11331112Pro-epidermal growth factor
PRO_0000007546Add
BLAST
Chaini974 – 102653Epidermal growth factor
PRO_0000007547Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi105 – 1051N-linked (GlcNAc...) Reviewed prediction
Glycosylationi118 – 1181N-linked (GlcNAc...) Reviewed prediction
Glycosylationi149 – 1491N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi361 ↔ 372 By similarity
Disulfide bondi368 ↔ 381 By similarity
Disulfide bondi383 ↔ 396 By similarity
Disulfide bondi402 ↔ 413 By similarity
Glycosylationi405 – 4051N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi409 ↔ 422 By similarity
Disulfide bondi424 ↔ 437 By similarity
Disulfide bondi440 ↔ 452 By similarity
Disulfide bondi448 ↔ 462 By similarity
Disulfide bondi464 ↔ 477 By similarity
Disulfide bondi747 ↔ 758 By similarity
Disulfide bondi754 ↔ 767 By similarity
Disulfide bondi769 ↔ 782 By similarity
Disulfide bondi839 ↔ 850 By similarity
Disulfide bondi844 ↔ 859 By similarity
Disulfide bondi861 ↔ 872 By similarity
Disulfide bondi878 ↔ 892 By similarity
Disulfide bondi885 ↔ 901 By similarity
Disulfide bondi903 ↔ 914 By similarity
Disulfide bondi920 ↔ 933 By similarity
Disulfide bondi927 ↔ 942 By similarity
Glycosylationi930 – 9301N-linked (GlcNAc...) Reviewed prediction
Glycosylationi941 – 9411N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi944 ↔ 955 By similarity
Disulfide bondi979 ↔ 993
Disulfide bondi987 ↔ 1004
Disulfide bondi1006 ↔ 1015

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP07522.

PTM databases

PhosphoSiteiP07522.

Expressioni

Gene expression databases

GenevestigatoriP07522.

Interactioni

Subunit structurei

Interacts with EGFR and promotes EGFR dimerization. Interacts with RHBDF1; may retain EGF in the endoplasmic reticulum and regulates its degradation through the endoplasmic reticulum-associated degradation (ERAD) By similarity. Interacts with RHBDF2 By similarity.

Structurei

3D structure databases

ProteinModelPortaliP07522.
SMRiP07522. Positions 974-1026.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati87 – 12842LDL-receptor class B 1
Add
BLAST
Repeati129 – 17042LDL-receptor class B 2
Add
BLAST
Repeati171 – 21242LDL-receptor class B 3
Add
BLAST
Repeati213 – 25947LDL-receptor class B 4
Add
BLAST
Domaini322 – 35635EGF-like 1; incomplete
Add
BLAST
Domaini357 – 39741EGF-like 2; calcium-binding Reviewed prediction
Add
BLAST
Domaini398 – 43841EGF-like 3
Add
BLAST
Domaini436 – 47843EGF-like 4
Add
BLAST
Repeati485 – 52541LDL-receptor class B 5
Add
BLAST
Repeati526 – 56843LDL-receptor class B 6
Add
BLAST
Repeati569 – 61143LDL-receptor class B 7
Add
BLAST
Repeati612 – 65544LDL-receptor class B 8
Add
BLAST
Repeati656 – 69843LDL-receptor class B 9
Add
BLAST
Domaini743 – 78341EGF-like 5
Add
BLAST
Domaini835 – 87339EGF-like 6
Add
BLAST
Domaini874 – 91542EGF-like 7; calcium-binding Reviewed prediction
Add
BLAST
Domaini916 – 95641EGF-like 8; calcium-binding Reviewed prediction
Add
BLAST
Domaini975 – 101642EGF-like 9
Add
BLAST

Sequence similaritiesi

Contains 9 EGF-like domains.

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG325841.
HOGENOMiHOG000112345.
HOVERGENiHBG003858.
InParanoidiP07522.
KOiK04357.
PhylomeDBiP07522.

Family and domain databases

Gene3Di2.120.10.30. 2 hits.
2.40.155.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR023413. GFP_like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000033. LDLR_classB_rpt.
IPR016317. Pro-epidermal_GF.
[Graphical view]
PfamiPF07645. EGF_CA. 3 hits.
PF00058. Ldl_recept_b. 3 hits.
[Graphical view]
PIRSFiPIRSF001778. Pro-epidermal_growth_factor. 1 hit.
SMARTiSM00181. EGF. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00135. LY. 8 hits.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 3 hits.
PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 3 hits.
PS51120. LDLRB. 9 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07522-1 [UniParc]FASTAAdd to Basket

« Hide

MLFSLTFLSV FLKITVLSVT AQQTRNCQSG PLERSGTTTY AAAGPPRFLI     50
FLQGNSIFRI NTDGTNHQQL VVDAGVSVVM DFHYKEERLY WVDLERQLLQ 100
RVFFNGSGQE TVCKVDKNVS GLAINWIDGE ILRTDRWKGV ITVTDMNGNN 150
SRVLLSSLKR PANILVDPTE RLIFWSSVVT GNLHRADLGG MDVKTLLEAP 200
ERISVLILDI LDKRLFWAQD GREGSHGYIH SCDYNGGSIH HIRHQARHDL 250
LTMAIFGDKI LYSALKEKAI WIADKHTGKN VVRVNLDPAS VPPRELRVVH 300
LHAQPGTENR AQASDSERCK QRRGQCLYSL SERDPNSDSS ACAEGYTLSR 350
DRKYCEDVNE CALQNHGCTL GCENIPGSYY CTCPTGFVLL PDGKRCHELV 400
ACPGNRSECS HDCILTSDGP LCICPAGSVL GKDGKTCTGC SFSDNGGCSQ 450
ICLPLSLASW ECDCFPGYDL QLDRKSCAAS MGPQPFLLFA NSQDIRHMHF 500
DGTDYKTLLS RQMGMVFALD YDPVESKIYF AQTALKWIER ANLDGSQRER 550
RITEGVDTPE GLAVDWIGRR IYWTDSGKSV IEGSDLSGKH HQIIIKESIS 600
RPRGIAVHPK ARRLFWTDTG MSPRIESSSL QGSDRTLIAS SNLLEPSGIA 650
IDYLTDTLYW CDTKLSVIEM ADLDGSKRRR LTQNDVGHPF SLAVFEDHVW 700
FSDWAIPSVI RVNKRTGQNR VRLRGSMLKP SSLVVVHPLA KPGADPCLHR 750
NGGCEHICQE SLGTAQCLCR EGFVKAPDGK MCLTRKDDQI LAGDNADLSK 800
EVASLDNSPK AYVPDDDRTE SSTLVAEIMV SGLNYEDDCG PGGCGSHAHC 850
ISEGEAAVCQ CLKGFAGDGN LCSDIDECEL GSSDCPPTSS RCINTEGGYV 900
CQCSEGYEGD GIYCLDVDEC QQGSHGCSEN ATCTNTEGGY NCTCAGCPSA 950
PGLPCPDSTS PSLLGKDGCH WVRNSNTGCP PSYDGYCLNG GVCMYVESVD 1000
RYVCNCVIGY IGERCQHRDL RWWKLRHADY GQRHDITVVS VCVVALALLL 1050
LLGMWGTYYY RTRKQLSESS KKPSEESSSN VSSNGPDSSG AGVSSGPQPW 1100
FVVLEEHQQP KNGRLPAAGT NGAVVEAGLS SSL 1133
Length:1,133
Mass (Da):124,126
Last modified:February 1, 1996 - v2
Checksum:iC224A302E9578031
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti955 – 9551C → V.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1024 – 10252KL → NW in CAA31241. 1 Publication
Sequence conflicti1109 – 113325QPKNG…LSSSL → SGAGVSSGPQPWFVVLEEHQ in CAA31241. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04842 mRNA. Translation: AAB60436.1.
X12748 mRNA. Translation: CAA31241.1.
PIRiI52995. EGRT.
RefSeqiNP_036974.1. NM_012842.1.
UniGeneiRn.6075.

Genome annotation databases

GeneIDi25313.
KEGGirno:25313.
UCSCiRGD:2542. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04842 mRNA. Translation: AAB60436.1 .
X12748 mRNA. Translation: CAA31241.1 .
PIRi I52995. EGRT.
RefSeqi NP_036974.1. NM_012842.1.
UniGenei Rn.6075.

3D structure databases

ProteinModelPortali P07522.
SMRi P07522. Positions 974-1026.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P07522.

PTM databases

PhosphoSitei P07522.

Proteomic databases

PRIDEi P07522.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25313.
KEGGi rno:25313.
UCSCi RGD:2542. rat.

Organism-specific databases

CTDi 1950.
RGDi 2542. Egf.

Phylogenomic databases

eggNOGi NOG325841.
HOGENOMi HOG000112345.
HOVERGENi HBG003858.
InParanoidi P07522.
KOi K04357.
PhylomeDBi P07522.

Miscellaneous databases

NextBioi 606131.
PROi P07522.

Gene expression databases

Genevestigatori P07522.

Family and domain databases

Gene3Di 2.120.10.30. 2 hits.
2.40.155.10. 1 hit.
InterProi IPR011042. 6-blade_b-propeller_TolB-like.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR023413. GFP_like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000033. LDLR_classB_rpt.
IPR016317. Pro-epidermal_GF.
[Graphical view ]
Pfami PF07645. EGF_CA. 3 hits.
PF00058. Ldl_recept_b. 3 hits.
[Graphical view ]
PIRSFi PIRSF001778. Pro-epidermal_growth_factor. 1 hit.
SMARTi SM00181. EGF. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00135. LY. 8 hits.
[Graphical view ]
SUPFAMi SSF57184. SSF57184. 3 hits.
PROSITEi PS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 3 hits.
PS51120. LDLRB. 9 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of the rat preproepidermal growth factor cDNA: comparison with mouse and human sequences."
    Price P.M., Saggi S.J., Safirstein R.
    DNA Cell Biol. 11:481-487(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. Price P.M.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
    Tissue: Kidney.
  3. "Rat epidermal growth factor: complete amino acid sequence. Homology with the corresponding murine and human proteins; isolation of a form truncated at both ends with full in vitro biological activity."
    Simpson R.J., Smith J.A., Moritz R.L., O'Hare M.J., Rudland P.S., Morrison J.R., Lloyd C.J., Grego B., Burgess A.W., Nice E.C.
    Eur. J. Biochem. 153:629-637(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 974-1021.
  4. "Cloning and sequence analysis of a cDNA for rat epidermal growth factor."
    Dorow D.S., Simpson R.J.
    Nucleic Acids Res. 16:9338-9338(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 994-1133.
    Strain: Sprague-Dawley.
    Tissue: Kidney.

Entry informationi

Entry nameiEGF_RAT
AccessioniPrimary (citable) accession number: P07522
Secondary accession number(s): Q63183
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi