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P07522

- EGF_RAT

UniProt

P07522 - EGF_RAT

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Protein

Pro-epidermal growth factor

Gene

Egf

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 By similarity.By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. epidermal growth factor receptor binding Source: RGD

GO - Biological processi

  1. epidermal growth factor receptor signaling pathway Source: RGD
  2. intracellular signal transduction Source: RGD
  3. positive regulation of DNA binding Source: UniProtKB
  4. regulation of protein secretion Source: RGD
  5. regulation of protein transport Source: MGI
  6. STAT protein import into nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-epidermal growth factor
Short name:
EGF
Cleaved into the following chain:
Gene namesi
Name:Egf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2542. Egf.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 11331112Pro-epidermal growth factorPRO_0000007546Add
BLAST
Chaini974 – 102653Epidermal growth factorPRO_0000007547Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi361 ↔ 372PROSITE-ProRule annotation
Disulfide bondi368 ↔ 381PROSITE-ProRule annotation
Disulfide bondi383 ↔ 396PROSITE-ProRule annotation
Disulfide bondi402 ↔ 413PROSITE-ProRule annotation
Glycosylationi405 – 4051N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi409 ↔ 422PROSITE-ProRule annotation
Disulfide bondi424 ↔ 437PROSITE-ProRule annotation
Disulfide bondi440 ↔ 452PROSITE-ProRule annotation
Disulfide bondi448 ↔ 462PROSITE-ProRule annotation
Disulfide bondi464 ↔ 477PROSITE-ProRule annotation
Disulfide bondi747 ↔ 758PROSITE-ProRule annotation
Disulfide bondi754 ↔ 767PROSITE-ProRule annotation
Disulfide bondi769 ↔ 782PROSITE-ProRule annotation
Disulfide bondi839 ↔ 850PROSITE-ProRule annotation
Disulfide bondi844 ↔ 859PROSITE-ProRule annotation
Disulfide bondi861 ↔ 872PROSITE-ProRule annotation
Disulfide bondi878 ↔ 892PROSITE-ProRule annotation
Disulfide bondi885 ↔ 901PROSITE-ProRule annotation
Disulfide bondi903 ↔ 914PROSITE-ProRule annotation
Disulfide bondi920 ↔ 933PROSITE-ProRule annotation
Disulfide bondi927 ↔ 942PROSITE-ProRule annotation
Glycosylationi930 – 9301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi941 – 9411N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi944 ↔ 955PROSITE-ProRule annotation
Disulfide bondi979 ↔ 993
Disulfide bondi987 ↔ 1004
Disulfide bondi1006 ↔ 1015

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP07522.

PTM databases

PhosphoSiteiP07522.

Expressioni

Gene expression databases

GenevestigatoriP07522.

Interactioni

Subunit structurei

Interacts with EGFR and promotes EGFR dimerization. Interacts with RHBDF1; may retain EGF in the endoplasmic reticulum and regulates its degradation through the endoplasmic reticulum-associated degradation (ERAD) By similarity. Interacts with RHBDF2 By similarity.By similarity

Structurei

3D structure databases

ProteinModelPortaliP07522.
SMRiP07522. Positions 974-1026.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 10351014ExtracellularSequence AnalysisAdd
BLAST
Topological domaini1058 – 113376CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1036 – 105722HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati87 – 12842LDL-receptor class B 1Add
BLAST
Repeati129 – 17042LDL-receptor class B 2Add
BLAST
Repeati171 – 21242LDL-receptor class B 3Add
BLAST
Repeati213 – 25947LDL-receptor class B 4Add
BLAST
Domaini322 – 35635EGF-like 1; incompletePROSITE-ProRule annotationAdd
BLAST
Domaini357 – 39741EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini398 – 43841EGF-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini436 – 47843EGF-like 4PROSITE-ProRule annotationAdd
BLAST
Repeati485 – 52541LDL-receptor class B 5Add
BLAST
Repeati526 – 56843LDL-receptor class B 6Add
BLAST
Repeati569 – 61143LDL-receptor class B 7Add
BLAST
Repeati612 – 65544LDL-receptor class B 8Add
BLAST
Repeati656 – 69843LDL-receptor class B 9Add
BLAST
Domaini743 – 78341EGF-like 5PROSITE-ProRule annotationAdd
BLAST
Domaini835 – 87339EGF-like 6PROSITE-ProRule annotationAdd
BLAST
Domaini874 – 91542EGF-like 7; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini916 – 95641EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini975 – 101642EGF-like 9PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 9 EGF-like domains.PROSITE-ProRule annotation
Contains 9 LDL-receptor class B repeats.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG325841.
HOGENOMiHOG000112345.
HOVERGENiHBG003858.
InParanoidiP07522.
KOiK04357.
PhylomeDBiP07522.

Family and domain databases

Gene3Di2.120.10.30. 2 hits.
2.40.155.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR023413. GFP_like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000033. LDLR_classB_rpt.
IPR016317. Pro-epidermal_GF.
[Graphical view]
PfamiPF07645. EGF_CA. 3 hits.
PF00058. Ldl_recept_b. 3 hits.
[Graphical view]
PIRSFiPIRSF001778. Pro-epidermal_growth_factor. 1 hit.
SMARTiSM00181. EGF. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00135. LY. 8 hits.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 3 hits.
PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 3 hits.
PS51120. LDLRB. 9 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07522-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLFSLTFLSV FLKITVLSVT AQQTRNCQSG PLERSGTTTY AAAGPPRFLI
60 70 80 90 100
FLQGNSIFRI NTDGTNHQQL VVDAGVSVVM DFHYKEERLY WVDLERQLLQ
110 120 130 140 150
RVFFNGSGQE TVCKVDKNVS GLAINWIDGE ILRTDRWKGV ITVTDMNGNN
160 170 180 190 200
SRVLLSSLKR PANILVDPTE RLIFWSSVVT GNLHRADLGG MDVKTLLEAP
210 220 230 240 250
ERISVLILDI LDKRLFWAQD GREGSHGYIH SCDYNGGSIH HIRHQARHDL
260 270 280 290 300
LTMAIFGDKI LYSALKEKAI WIADKHTGKN VVRVNLDPAS VPPRELRVVH
310 320 330 340 350
LHAQPGTENR AQASDSERCK QRRGQCLYSL SERDPNSDSS ACAEGYTLSR
360 370 380 390 400
DRKYCEDVNE CALQNHGCTL GCENIPGSYY CTCPTGFVLL PDGKRCHELV
410 420 430 440 450
ACPGNRSECS HDCILTSDGP LCICPAGSVL GKDGKTCTGC SFSDNGGCSQ
460 470 480 490 500
ICLPLSLASW ECDCFPGYDL QLDRKSCAAS MGPQPFLLFA NSQDIRHMHF
510 520 530 540 550
DGTDYKTLLS RQMGMVFALD YDPVESKIYF AQTALKWIER ANLDGSQRER
560 570 580 590 600
RITEGVDTPE GLAVDWIGRR IYWTDSGKSV IEGSDLSGKH HQIIIKESIS
610 620 630 640 650
RPRGIAVHPK ARRLFWTDTG MSPRIESSSL QGSDRTLIAS SNLLEPSGIA
660 670 680 690 700
IDYLTDTLYW CDTKLSVIEM ADLDGSKRRR LTQNDVGHPF SLAVFEDHVW
710 720 730 740 750
FSDWAIPSVI RVNKRTGQNR VRLRGSMLKP SSLVVVHPLA KPGADPCLHR
760 770 780 790 800
NGGCEHICQE SLGTAQCLCR EGFVKAPDGK MCLTRKDDQI LAGDNADLSK
810 820 830 840 850
EVASLDNSPK AYVPDDDRTE SSTLVAEIMV SGLNYEDDCG PGGCGSHAHC
860 870 880 890 900
ISEGEAAVCQ CLKGFAGDGN LCSDIDECEL GSSDCPPTSS RCINTEGGYV
910 920 930 940 950
CQCSEGYEGD GIYCLDVDEC QQGSHGCSEN ATCTNTEGGY NCTCAGCPSA
960 970 980 990 1000
PGLPCPDSTS PSLLGKDGCH WVRNSNTGCP PSYDGYCLNG GVCMYVESVD
1010 1020 1030 1040 1050
RYVCNCVIGY IGERCQHRDL RWWKLRHADY GQRHDITVVS VCVVALALLL
1060 1070 1080 1090 1100
LLGMWGTYYY RTRKQLSESS KKPSEESSSN VSSNGPDSSG AGVSSGPQPW
1110 1120 1130
FVVLEEHQQP KNGRLPAAGT NGAVVEAGLS SSL
Length:1,133
Mass (Da):124,126
Last modified:February 1, 1996 - v2
Checksum:iC224A302E9578031
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1024 – 10252KL → NW in CAA31241. (PubMed:3262867)Curated
Sequence conflicti1109 – 113325QPKNG…LSSSL → SGAGVSSGPQPWFVVLEEHQ in CAA31241. (PubMed:3262867)CuratedAdd
BLAST

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti955 – 9551C → V.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04842 mRNA. Translation: AAB60436.1.
X12748 mRNA. Translation: CAA31241.1.
PIRiI52995. EGRT.
RefSeqiNP_036974.1. NM_012842.1.
UniGeneiRn.6075.

Genome annotation databases

GeneIDi25313.
KEGGirno:25313.
UCSCiRGD:2542. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04842 mRNA. Translation: AAB60436.1 .
X12748 mRNA. Translation: CAA31241.1 .
PIRi I52995. EGRT.
RefSeqi NP_036974.1. NM_012842.1.
UniGenei Rn.6075.

3D structure databases

ProteinModelPortali P07522.
SMRi P07522. Positions 974-1026.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P07522.

PTM databases

PhosphoSitei P07522.

Proteomic databases

PRIDEi P07522.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25313.
KEGGi rno:25313.
UCSCi RGD:2542. rat.

Organism-specific databases

CTDi 1950.
RGDi 2542. Egf.

Phylogenomic databases

eggNOGi NOG325841.
HOGENOMi HOG000112345.
HOVERGENi HBG003858.
InParanoidi P07522.
KOi K04357.
PhylomeDBi P07522.

Miscellaneous databases

NextBioi 606131.
PROi P07522.

Gene expression databases

Genevestigatori P07522.

Family and domain databases

Gene3Di 2.120.10.30. 2 hits.
2.40.155.10. 1 hit.
InterProi IPR011042. 6-blade_b-propeller_TolB-like.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR023413. GFP_like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000033. LDLR_classB_rpt.
IPR016317. Pro-epidermal_GF.
[Graphical view ]
Pfami PF07645. EGF_CA. 3 hits.
PF00058. Ldl_recept_b. 3 hits.
[Graphical view ]
PIRSFi PIRSF001778. Pro-epidermal_growth_factor. 1 hit.
SMARTi SM00181. EGF. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00135. LY. 8 hits.
[Graphical view ]
SUPFAMi SSF57184. SSF57184. 3 hits.
PROSITEi PS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 3 hits.
PS51120. LDLRB. 9 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of the rat preproepidermal growth factor cDNA: comparison with mouse and human sequences."
    Price P.M., Saggi S.J., Safirstein R.
    DNA Cell Biol. 11:481-487(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. Price P.M.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
    Tissue: Kidney.
  3. "Rat epidermal growth factor: complete amino acid sequence. Homology with the corresponding murine and human proteins; isolation of a form truncated at both ends with full in vitro biological activity."
    Simpson R.J., Smith J.A., Moritz R.L., O'Hare M.J., Rudland P.S., Morrison J.R., Lloyd C.J., Grego B., Burgess A.W., Nice E.C.
    Eur. J. Biochem. 153:629-637(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 974-1021.
  4. "Cloning and sequence analysis of a cDNA for rat epidermal growth factor."
    Dorow D.S., Simpson R.J.
    Nucleic Acids Res. 16:9338-9338(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 994-1133.
    Strain: Sprague-Dawley.
    Tissue: Kidney.

Entry informationi

Entry nameiEGF_RAT
AccessioniPrimary (citable) accession number: P07522
Secondary accession number(s): Q63183
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1996
Last modified: October 1, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3