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P07522

- EGF_RAT

UniProt

P07522 - EGF_RAT

Protein

Pro-epidermal growth factor

Gene

Egf

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 By similarity.By similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. epidermal growth factor receptor binding Source: RGD

    GO - Biological processi

    1. epidermal growth factor receptor signaling pathway Source: RGD
    2. intracellular signal transduction Source: RGD
    3. positive regulation of DNA binding Source: UniProtKB
    4. regulation of protein secretion Source: RGD
    5. regulation of protein transport Source: MGI
    6. STAT protein import into nucleus Source: UniProtKB

    Keywords - Molecular functioni

    Growth factor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pro-epidermal growth factor
    Short name:
    EGF
    Cleaved into the following chain:
    Gene namesi
    Name:Egf
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2542. Egf.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 11331112Pro-epidermal growth factorPRO_0000007546Add
    BLAST
    Chaini974 – 102653Epidermal growth factorPRO_0000007547Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi361 ↔ 372PROSITE-ProRule annotation
    Disulfide bondi368 ↔ 381PROSITE-ProRule annotation
    Disulfide bondi383 ↔ 396PROSITE-ProRule annotation
    Disulfide bondi402 ↔ 413PROSITE-ProRule annotation
    Glycosylationi405 – 4051N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi409 ↔ 422PROSITE-ProRule annotation
    Disulfide bondi424 ↔ 437PROSITE-ProRule annotation
    Disulfide bondi440 ↔ 452PROSITE-ProRule annotation
    Disulfide bondi448 ↔ 462PROSITE-ProRule annotation
    Disulfide bondi464 ↔ 477PROSITE-ProRule annotation
    Disulfide bondi747 ↔ 758PROSITE-ProRule annotation
    Disulfide bondi754 ↔ 767PROSITE-ProRule annotation
    Disulfide bondi769 ↔ 782PROSITE-ProRule annotation
    Disulfide bondi839 ↔ 850PROSITE-ProRule annotation
    Disulfide bondi844 ↔ 859PROSITE-ProRule annotation
    Disulfide bondi861 ↔ 872PROSITE-ProRule annotation
    Disulfide bondi878 ↔ 892PROSITE-ProRule annotation
    Disulfide bondi885 ↔ 901PROSITE-ProRule annotation
    Disulfide bondi903 ↔ 914PROSITE-ProRule annotation
    Disulfide bondi920 ↔ 933PROSITE-ProRule annotation
    Disulfide bondi927 ↔ 942PROSITE-ProRule annotation
    Glycosylationi930 – 9301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi941 – 9411N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi944 ↔ 955PROSITE-ProRule annotation
    Disulfide bondi979 ↔ 993
    Disulfide bondi987 ↔ 1004
    Disulfide bondi1006 ↔ 1015

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP07522.

    PTM databases

    PhosphoSiteiP07522.

    Expressioni

    Gene expression databases

    GenevestigatoriP07522.

    Interactioni

    Subunit structurei

    Interacts with EGFR and promotes EGFR dimerization. Interacts with RHBDF1; may retain EGF in the endoplasmic reticulum and regulates its degradation through the endoplasmic reticulum-associated degradation (ERAD) By similarity. Interacts with RHBDF2 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP07522.
    SMRiP07522. Positions 974-1026.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini22 – 10351014ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1058 – 113376CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1036 – 105722HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati87 – 12842LDL-receptor class B 1Add
    BLAST
    Repeati129 – 17042LDL-receptor class B 2Add
    BLAST
    Repeati171 – 21242LDL-receptor class B 3Add
    BLAST
    Repeati213 – 25947LDL-receptor class B 4Add
    BLAST
    Domaini322 – 35635EGF-like 1; incompletePROSITE-ProRule annotationAdd
    BLAST
    Domaini357 – 39741EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini398 – 43841EGF-like 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini436 – 47843EGF-like 4PROSITE-ProRule annotationAdd
    BLAST
    Repeati485 – 52541LDL-receptor class B 5Add
    BLAST
    Repeati526 – 56843LDL-receptor class B 6Add
    BLAST
    Repeati569 – 61143LDL-receptor class B 7Add
    BLAST
    Repeati612 – 65544LDL-receptor class B 8Add
    BLAST
    Repeati656 – 69843LDL-receptor class B 9Add
    BLAST
    Domaini743 – 78341EGF-like 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini835 – 87339EGF-like 6PROSITE-ProRule annotationAdd
    BLAST
    Domaini874 – 91542EGF-like 7; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini916 – 95641EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini975 – 101642EGF-like 9PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 9 EGF-like domains.PROSITE-ProRule annotation
    Contains 9 LDL-receptor class B repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG325841.
    HOGENOMiHOG000112345.
    HOVERGENiHBG003858.
    InParanoidiP07522.
    KOiK04357.
    PhylomeDBiP07522.

    Family and domain databases

    Gene3Di2.120.10.30. 2 hits.
    2.40.155.10. 1 hit.
    InterProiIPR011042. 6-blade_b-propeller_TolB-like.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR023413. GFP_like.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR000033. LDLR_classB_rpt.
    IPR016317. Pro-epidermal_GF.
    [Graphical view]
    PfamiPF07645. EGF_CA. 3 hits.
    PF00058. Ldl_recept_b. 3 hits.
    [Graphical view]
    PIRSFiPIRSF001778. Pro-epidermal_growth_factor. 1 hit.
    SMARTiSM00181. EGF. 5 hits.
    SM00179. EGF_CA. 2 hits.
    SM00135. LY. 8 hits.
    [Graphical view]
    SUPFAMiSSF57184. SSF57184. 3 hits.
    PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
    PS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 6 hits.
    PS50026. EGF_3. 5 hits.
    PS01187. EGF_CA. 3 hits.
    PS51120. LDLRB. 9 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07522-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLFSLTFLSV FLKITVLSVT AQQTRNCQSG PLERSGTTTY AAAGPPRFLI     50
    FLQGNSIFRI NTDGTNHQQL VVDAGVSVVM DFHYKEERLY WVDLERQLLQ 100
    RVFFNGSGQE TVCKVDKNVS GLAINWIDGE ILRTDRWKGV ITVTDMNGNN 150
    SRVLLSSLKR PANILVDPTE RLIFWSSVVT GNLHRADLGG MDVKTLLEAP 200
    ERISVLILDI LDKRLFWAQD GREGSHGYIH SCDYNGGSIH HIRHQARHDL 250
    LTMAIFGDKI LYSALKEKAI WIADKHTGKN VVRVNLDPAS VPPRELRVVH 300
    LHAQPGTENR AQASDSERCK QRRGQCLYSL SERDPNSDSS ACAEGYTLSR 350
    DRKYCEDVNE CALQNHGCTL GCENIPGSYY CTCPTGFVLL PDGKRCHELV 400
    ACPGNRSECS HDCILTSDGP LCICPAGSVL GKDGKTCTGC SFSDNGGCSQ 450
    ICLPLSLASW ECDCFPGYDL QLDRKSCAAS MGPQPFLLFA NSQDIRHMHF 500
    DGTDYKTLLS RQMGMVFALD YDPVESKIYF AQTALKWIER ANLDGSQRER 550
    RITEGVDTPE GLAVDWIGRR IYWTDSGKSV IEGSDLSGKH HQIIIKESIS 600
    RPRGIAVHPK ARRLFWTDTG MSPRIESSSL QGSDRTLIAS SNLLEPSGIA 650
    IDYLTDTLYW CDTKLSVIEM ADLDGSKRRR LTQNDVGHPF SLAVFEDHVW 700
    FSDWAIPSVI RVNKRTGQNR VRLRGSMLKP SSLVVVHPLA KPGADPCLHR 750
    NGGCEHICQE SLGTAQCLCR EGFVKAPDGK MCLTRKDDQI LAGDNADLSK 800
    EVASLDNSPK AYVPDDDRTE SSTLVAEIMV SGLNYEDDCG PGGCGSHAHC 850
    ISEGEAAVCQ CLKGFAGDGN LCSDIDECEL GSSDCPPTSS RCINTEGGYV 900
    CQCSEGYEGD GIYCLDVDEC QQGSHGCSEN ATCTNTEGGY NCTCAGCPSA 950
    PGLPCPDSTS PSLLGKDGCH WVRNSNTGCP PSYDGYCLNG GVCMYVESVD 1000
    RYVCNCVIGY IGERCQHRDL RWWKLRHADY GQRHDITVVS VCVVALALLL 1050
    LLGMWGTYYY RTRKQLSESS KKPSEESSSN VSSNGPDSSG AGVSSGPQPW 1100
    FVVLEEHQQP KNGRLPAAGT NGAVVEAGLS SSL 1133
    Length:1,133
    Mass (Da):124,126
    Last modified:February 1, 1996 - v2
    Checksum:iC224A302E9578031
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1024 – 10252KL → NW in CAA31241. (PubMed:3262867)Curated
    Sequence conflicti1109 – 113325QPKNG…LSSSL → SGAGVSSGPQPWFVVLEEHQ in CAA31241. (PubMed:3262867)CuratedAdd
    BLAST

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti955 – 9551C → V.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04842 mRNA. Translation: AAB60436.1.
    X12748 mRNA. Translation: CAA31241.1.
    PIRiI52995. EGRT.
    RefSeqiNP_036974.1. NM_012842.1.
    UniGeneiRn.6075.

    Genome annotation databases

    GeneIDi25313.
    KEGGirno:25313.
    UCSCiRGD:2542. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04842 mRNA. Translation: AAB60436.1 .
    X12748 mRNA. Translation: CAA31241.1 .
    PIRi I52995. EGRT.
    RefSeqi NP_036974.1. NM_012842.1.
    UniGenei Rn.6075.

    3D structure databases

    ProteinModelPortali P07522.
    SMRi P07522. Positions 974-1026.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P07522.

    PTM databases

    PhosphoSitei P07522.

    Proteomic databases

    PRIDEi P07522.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 25313.
    KEGGi rno:25313.
    UCSCi RGD:2542. rat.

    Organism-specific databases

    CTDi 1950.
    RGDi 2542. Egf.

    Phylogenomic databases

    eggNOGi NOG325841.
    HOGENOMi HOG000112345.
    HOVERGENi HBG003858.
    InParanoidi P07522.
    KOi K04357.
    PhylomeDBi P07522.

    Miscellaneous databases

    NextBioi 606131.
    PROi P07522.

    Gene expression databases

    Genevestigatori P07522.

    Family and domain databases

    Gene3Di 2.120.10.30. 2 hits.
    2.40.155.10. 1 hit.
    InterProi IPR011042. 6-blade_b-propeller_TolB-like.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR023413. GFP_like.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR000033. LDLR_classB_rpt.
    IPR016317. Pro-epidermal_GF.
    [Graphical view ]
    Pfami PF07645. EGF_CA. 3 hits.
    PF00058. Ldl_recept_b. 3 hits.
    [Graphical view ]
    PIRSFi PIRSF001778. Pro-epidermal_growth_factor. 1 hit.
    SMARTi SM00181. EGF. 5 hits.
    SM00179. EGF_CA. 2 hits.
    SM00135. LY. 8 hits.
    [Graphical view ]
    SUPFAMi SSF57184. SSF57184. 3 hits.
    PROSITEi PS00010. ASX_HYDROXYL. 3 hits.
    PS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 6 hits.
    PS50026. EGF_3. 5 hits.
    PS01187. EGF_CA. 3 hits.
    PS51120. LDLRB. 9 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of the rat preproepidermal growth factor cDNA: comparison with mouse and human sequences."
      Price P.M., Saggi S.J., Safirstein R.
      DNA Cell Biol. 11:481-487(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    2. Price P.M.
      Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
      Tissue: Kidney.
    3. "Rat epidermal growth factor: complete amino acid sequence. Homology with the corresponding murine and human proteins; isolation of a form truncated at both ends with full in vitro biological activity."
      Simpson R.J., Smith J.A., Moritz R.L., O'Hare M.J., Rudland P.S., Morrison J.R., Lloyd C.J., Grego B., Burgess A.W., Nice E.C.
      Eur. J. Biochem. 153:629-637(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 974-1021.
    4. "Cloning and sequence analysis of a cDNA for rat epidermal growth factor."
      Dorow D.S., Simpson R.J.
      Nucleic Acids Res. 16:9338-9338(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 994-1133.
      Strain: Sprague-Dawley.
      Tissue: Kidney.

    Entry informationi

    Entry nameiEGF_RAT
    AccessioniPrimary (citable) accession number: P07522
    Secondary accession number(s): Q63183
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3