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Protein

Pro-epidermal growth factor

Gene

Egf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • canonical Wnt signaling pathway Source: GO_Central
  • epidermal growth factor receptor signaling pathway Source: RGD
  • intracellular signal transduction Source: RGD
  • kidney development Source: RGD
  • positive regulation of DNA binding Source: UniProtKB
  • positive regulation of DNA biosynthetic process Source: RGD
  • positive regulation of fibroblast proliferation Source: RGD
  • regulation of protein secretion Source: RGD
  • regulation of protein transport Source: MGI
  • STAT protein import into nucleus Source: UniProtKB
  • Wnt signaling pathway involved in dorsal/ventral axis specification Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-epidermal growth factor
Short name:
EGF
Cleaved into the following chain:
Gene namesi
Name:Egf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2542. Egf.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 1035ExtracellularSequence analysisAdd BLAST1014
Transmembranei1036 – 1057HelicalSequence analysisAdd BLAST22
Topological domaini1058 – 1133CytoplasmicSequence analysisAdd BLAST76

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000754622 – 1133Pro-epidermal growth factorAdd BLAST1112
ChainiPRO_0000007547974 – 1026Epidermal growth factorAdd BLAST53

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi105N-linked (GlcNAc...)Sequence analysis1
Glycosylationi118N-linked (GlcNAc...)Sequence analysis1
Glycosylationi149N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi361 ↔ 372PROSITE-ProRule annotation
Disulfide bondi368 ↔ 381PROSITE-ProRule annotation
Disulfide bondi383 ↔ 396PROSITE-ProRule annotation
Disulfide bondi402 ↔ 413PROSITE-ProRule annotation
Glycosylationi405N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi409 ↔ 422PROSITE-ProRule annotation
Disulfide bondi424 ↔ 437PROSITE-ProRule annotation
Disulfide bondi440 ↔ 452PROSITE-ProRule annotation
Disulfide bondi448 ↔ 462PROSITE-ProRule annotation
Disulfide bondi464 ↔ 477PROSITE-ProRule annotation
Disulfide bondi747 ↔ 758PROSITE-ProRule annotation
Disulfide bondi754 ↔ 767PROSITE-ProRule annotation
Disulfide bondi769 ↔ 782PROSITE-ProRule annotation
Disulfide bondi839 ↔ 850PROSITE-ProRule annotation
Disulfide bondi844 ↔ 859PROSITE-ProRule annotation
Disulfide bondi861 ↔ 872PROSITE-ProRule annotation
Disulfide bondi878 ↔ 892PROSITE-ProRule annotation
Disulfide bondi885 ↔ 901PROSITE-ProRule annotation
Disulfide bondi903 ↔ 914PROSITE-ProRule annotation
Disulfide bondi920 ↔ 933PROSITE-ProRule annotation
Disulfide bondi927 ↔ 942PROSITE-ProRule annotation
Glycosylationi930N-linked (GlcNAc...)Sequence analysis1
Glycosylationi941N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi944 ↔ 955PROSITE-ProRule annotation
Disulfide bondi979 ↔ 993
Disulfide bondi987 ↔ 1004
Disulfide bondi1006 ↔ 1015

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP07522.
PRIDEiP07522.

PTM databases

iPTMnetiP07522.
PhosphoSitePlusiP07522.

Interactioni

Subunit structurei

Interacts with EGFR and promotes EGFR dimerization. Interacts with RHBDF1; may retain EGF in the endoplasmic reticulum and regulates its degradation through the endoplasmic reticulum-associated degradation (ERAD) (By similarity). Interacts with RHBDF2 (By similarity).By similarity

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: RGD
  • Wnt-protein binding Source: GO_Central

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040920.

Chemistry databases

BindingDBiP07522.

Structurei

3D structure databases

ProteinModelPortaliP07522.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati87 – 128LDL-receptor class B 1Add BLAST42
Repeati129 – 170LDL-receptor class B 2Add BLAST42
Repeati171 – 212LDL-receptor class B 3Add BLAST42
Repeati213 – 259LDL-receptor class B 4Add BLAST47
Domaini322 – 356EGF-like 1; incompletePROSITE-ProRule annotationAdd BLAST35
Domaini357 – 397EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini398 – 438EGF-like 3PROSITE-ProRule annotationAdd BLAST41
Domaini436 – 478EGF-like 4PROSITE-ProRule annotationAdd BLAST43
Repeati485 – 525LDL-receptor class B 5Add BLAST41
Repeati526 – 568LDL-receptor class B 6Add BLAST43
Repeati569 – 611LDL-receptor class B 7Add BLAST43
Repeati612 – 655LDL-receptor class B 8Add BLAST44
Repeati656 – 698LDL-receptor class B 9Add BLAST43
Domaini743 – 783EGF-like 5PROSITE-ProRule annotationAdd BLAST41
Domaini835 – 873EGF-like 6PROSITE-ProRule annotationAdd BLAST39
Domaini874 – 915EGF-like 7; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini916 – 956EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini975 – 1016EGF-like 9PROSITE-ProRule annotationAdd BLAST42

Sequence similaritiesi

Contains 9 EGF-like domains.PROSITE-ProRule annotation
Contains 9 LDL-receptor class B repeats.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1215. Eukaryota.
ENOG410XP34. LUCA.
HOGENOMiHOG000112345.
HOVERGENiHBG003858.
InParanoidiP07522.
KOiK04357.
PhylomeDBiP07522.

Family and domain databases

Gene3Di2.120.10.30. 2 hits.
2.40.155.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR023413. GFP-like.
IPR009030. Growth_fac_rcpt_.
IPR000033. LDLR_classB_rpt.
IPR016317. Pro-epidermal_GF.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF07645. EGF_CA. 3 hits.
PF00058. Ldl_recept_b. 3 hits.
[Graphical view]
PIRSFiPIRSF001778. Pro-epidermal_growth_factor. 1 hit.
SMARTiSM00181. EGF. 8 hits.
SM00179. EGF_CA. 5 hits.
SM00135. LY. 8 hits.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 3 hits.
PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 3 hits.
PS51120. LDLRB. 9 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFSLTFLSV FLKITVLSVT AQQTRNCQSG PLERSGTTTY AAAGPPRFLI
60 70 80 90 100
FLQGNSIFRI NTDGTNHQQL VVDAGVSVVM DFHYKEERLY WVDLERQLLQ
110 120 130 140 150
RVFFNGSGQE TVCKVDKNVS GLAINWIDGE ILRTDRWKGV ITVTDMNGNN
160 170 180 190 200
SRVLLSSLKR PANILVDPTE RLIFWSSVVT GNLHRADLGG MDVKTLLEAP
210 220 230 240 250
ERISVLILDI LDKRLFWAQD GREGSHGYIH SCDYNGGSIH HIRHQARHDL
260 270 280 290 300
LTMAIFGDKI LYSALKEKAI WIADKHTGKN VVRVNLDPAS VPPRELRVVH
310 320 330 340 350
LHAQPGTENR AQASDSERCK QRRGQCLYSL SERDPNSDSS ACAEGYTLSR
360 370 380 390 400
DRKYCEDVNE CALQNHGCTL GCENIPGSYY CTCPTGFVLL PDGKRCHELV
410 420 430 440 450
ACPGNRSECS HDCILTSDGP LCICPAGSVL GKDGKTCTGC SFSDNGGCSQ
460 470 480 490 500
ICLPLSLASW ECDCFPGYDL QLDRKSCAAS MGPQPFLLFA NSQDIRHMHF
510 520 530 540 550
DGTDYKTLLS RQMGMVFALD YDPVESKIYF AQTALKWIER ANLDGSQRER
560 570 580 590 600
RITEGVDTPE GLAVDWIGRR IYWTDSGKSV IEGSDLSGKH HQIIIKESIS
610 620 630 640 650
RPRGIAVHPK ARRLFWTDTG MSPRIESSSL QGSDRTLIAS SNLLEPSGIA
660 670 680 690 700
IDYLTDTLYW CDTKLSVIEM ADLDGSKRRR LTQNDVGHPF SLAVFEDHVW
710 720 730 740 750
FSDWAIPSVI RVNKRTGQNR VRLRGSMLKP SSLVVVHPLA KPGADPCLHR
760 770 780 790 800
NGGCEHICQE SLGTAQCLCR EGFVKAPDGK MCLTRKDDQI LAGDNADLSK
810 820 830 840 850
EVASLDNSPK AYVPDDDRTE SSTLVAEIMV SGLNYEDDCG PGGCGSHAHC
860 870 880 890 900
ISEGEAAVCQ CLKGFAGDGN LCSDIDECEL GSSDCPPTSS RCINTEGGYV
910 920 930 940 950
CQCSEGYEGD GIYCLDVDEC QQGSHGCSEN ATCTNTEGGY NCTCAGCPSA
960 970 980 990 1000
PGLPCPDSTS PSLLGKDGCH WVRNSNTGCP PSYDGYCLNG GVCMYVESVD
1010 1020 1030 1040 1050
RYVCNCVIGY IGERCQHRDL RWWKLRHADY GQRHDITVVS VCVVALALLL
1060 1070 1080 1090 1100
LLGMWGTYYY RTRKQLSESS KKPSEESSSN VSSNGPDSSG AGVSSGPQPW
1110 1120 1130
FVVLEEHQQP KNGRLPAAGT NGAVVEAGLS SSL
Length:1,133
Mass (Da):124,126
Last modified:February 1, 1996 - v2
Checksum:iC224A302E9578031
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1024 – 1025KL → NW in CAA31241 (PubMed:3262867).Curated2
Sequence conflicti1109 – 1133QPKNG…LSSSL → SGAGVSSGPQPWFVVLEEHQ in CAA31241 (PubMed:3262867).CuratedAdd BLAST25

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti955C → V.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04842 mRNA. Translation: AAB60436.1.
X12748 mRNA. Translation: CAA31241.1.
PIRiI52995. EGRT.
RefSeqiNP_036974.1. NM_012842.1.
UniGeneiRn.6075.

Genome annotation databases

GeneIDi25313.
KEGGirno:25313.
UCSCiRGD:2542. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04842 mRNA. Translation: AAB60436.1.
X12748 mRNA. Translation: CAA31241.1.
PIRiI52995. EGRT.
RefSeqiNP_036974.1. NM_012842.1.
UniGeneiRn.6075.

3D structure databases

ProteinModelPortaliP07522.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040920.

Chemistry databases

BindingDBiP07522.

PTM databases

iPTMnetiP07522.
PhosphoSitePlusiP07522.

Proteomic databases

PaxDbiP07522.
PRIDEiP07522.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25313.
KEGGirno:25313.
UCSCiRGD:2542. rat.

Organism-specific databases

CTDi1950.
RGDi2542. Egf.

Phylogenomic databases

eggNOGiKOG1215. Eukaryota.
ENOG410XP34. LUCA.
HOGENOMiHOG000112345.
HOVERGENiHBG003858.
InParanoidiP07522.
KOiK04357.
PhylomeDBiP07522.

Miscellaneous databases

PROiP07522.

Family and domain databases

Gene3Di2.120.10.30. 2 hits.
2.40.155.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR023413. GFP-like.
IPR009030. Growth_fac_rcpt_.
IPR000033. LDLR_classB_rpt.
IPR016317. Pro-epidermal_GF.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF07645. EGF_CA. 3 hits.
PF00058. Ldl_recept_b. 3 hits.
[Graphical view]
PIRSFiPIRSF001778. Pro-epidermal_growth_factor. 1 hit.
SMARTiSM00181. EGF. 8 hits.
SM00179. EGF_CA. 5 hits.
SM00135. LY. 8 hits.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 3 hits.
PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 3 hits.
PS51120. LDLRB. 9 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEGF_RAT
AccessioniPrimary (citable) accession number: P07522
Secondary accession number(s): Q63183
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.