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Protein

Serine carboxypeptidase 1

Gene

CBP1

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in the degradation of small peptides (2-5 residues) or in the degradation of storage proteins in the embryo.

Catalytic activityi

Release of a C-terminal amino acid with broad specificity.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei188By similarity1
Active sitei423By similarity1
Active sitei476By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease

Protein family/group databases

ESTHERihorvu-cbp1 Carboxypeptidase_S10
MEROPSiS10.004

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase 1 (EC:3.4.16.5)
Alternative name(s):
CP-MI
Carboxypeptidase C
Serine carboxypeptidase I
Cleaved into the following 2 chains:
Alternative name(s):
Serine carboxypeptidase I chain A
Alternative name(s):
Serine carboxypeptidase I chain B
Gene namesi
Name:CBP1
Synonyms:CXP;1
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000000430131 – 296Serine carboxypeptidase 1 chain AAdd BLAST266
PropeptideiPRO_0000004302297 – 351Linker peptide1 PublicationAdd BLAST55
ChainiPRO_0000004303352 – 499Serine carboxypeptidase 1 chain BAdd BLAST148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi92 ↔ 388Interchain (between A and B chains)By similarity
Glycosylationi148N-linked (GlcNAc...) asparagine1
Disulfide bondi256 ↔ 268By similarity
Glycosylationi262N-linked (GlcNAc...) asparagine1
Disulfide bondi291 ↔ 355Interchain (between A and B chains)By similarity
Glycosylationi407N-linked (GlcNAc...) asparagine1

Post-translational modificationi

The linker peptide is endoproteolytically excised during enzyme maturation.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Developmental stagei

After one day of germination, mainly found in the scutellum of the developing grain; barely detectable after four days, and absent from the mature grain. A lower level of expression is seen in the aleurone both during development and germination.

Gene expression databases

ExpressionAtlasiP07519 differential

Interactioni

Subunit structurei

Carboxypeptidase I is a dimer, where each monomer is composed of two chains linked by disulfide bonds.

Structurei

3D structure databases

ProteinModelPortaliP07519
SMRiP07519
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi497 – 499Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1282 Eukaryota
COG2939 LUCA

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR001563 Peptidase_S10
IPR033124 Ser_caboxypep_his_AS
IPR018202 Ser_caboxypep_ser_AS
PANTHERiPTHR11802 PTHR11802, 1 hit
PfamiView protein in Pfam
PF00450 Peptidase_S10, 1 hit
PRINTSiPR00724 CRBOXYPTASEC
SUPFAMiSSF53474 SSF53474, 2 hits
PROSITEiView protein in PROSITE
PS00560 CARBOXYPEPT_SER_HIS, 1 hit
PS00131 CARBOXYPEPT_SER_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07519-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARCRRRSGC TAGAALLLLL ALALSGGGGA APQGAEVTGL PGFDGALPSK
60 70 80 90 100
HYAGYVTVDE GHGRNLFYYV VESERDPGKD PVVLWLNGGP GCSSFDGFVY
110 120 130 140 150
EHGPFNFESG GSVKSLPKLH LNPYAWSKVS TMIYLDSPAG VGLSYSKNVS
160 170 180 190 200
DYETGDLKTA TDSHTFLLKW FQLYPEFLSN PFYIAGESYA GVYVPTLSHE
210 220 230 240 250
VVKGIQGGAK PTINFKGYMV GNGVCDTIFD GNALVPFAHG MGLISDEIYQ
260 270 280 290 300
QASTSCHGNY WNATDGKCDT AISKIESLIS GLNIYDILEP CYHSRSIKEV
310 320 330 340 350
NLQNSKLPQS FKDLGTTNKP FPVRTRMLGR AWPLRAPVKA GRVPSWQEVA
360 370 380 390 400
SGVPCMSDEV ATAWLDNAAV RSAIHAQSVS AIGPWLLCTD KLYFVHDAGS
410 420 430 440 450
MIAYHKNLTS QGYRAIIFSG DHDMCVPFTG SEAWTKSLGY GVVDSWRPWI
460 470 480 490
TNGQVSGYTE GYEHGLTFAT IKGAGHTVPE YKPQEAFAFY SRWLAGSKL
Length:499
Mass (Da):54,096
Last modified:November 1, 1997 - v4
Checksum:i9C6674B14D9DB9BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102H → P AA sequence (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09603 mRNA Translation: CAA70816.1
J03897 mRNA Translation: AAA32940.1
PIRiT05367 CPBHS
UniGeneiHv.12403

Similar proteinsi

Entry informationi

Entry nameiCBP1_HORVU
AccessioniPrimary (citable) accession number: P07519
Secondary accession number(s): P07520
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 116 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing
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Main funding by: National Institutes of Health