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P07519

- CBP1_HORVU

UniProt

P07519 - CBP1_HORVU

Protein

Serine carboxypeptidase 1

Gene

CBP1

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 4 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    May be involved in the degradation of small peptides (2-5 residues) or in the degradation of storage proteins in the embryo.

    Catalytic activityi

    Release of a C-terminal amino acid with broad specificity.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei188 – 1881By similarity
    Active sitei423 – 4231By similarity
    Active sitei476 – 4761By similarity

    GO - Molecular functioni

    1. serine-type carboxypeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Carboxypeptidase, Hydrolase, Protease

    Protein family/group databases

    MEROPSiS10.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine carboxypeptidase 1 (EC:3.4.16.5)
    Alternative name(s):
    CP-MI
    Carboxypeptidase C
    Serine carboxypeptidase I
    Cleaved into the following 2 chains:
    Alternative name(s):
    Serine carboxypeptidase I chain A
    Alternative name(s):
    Serine carboxypeptidase I chain B
    Gene namesi
    Name:CBP1
    Synonyms:CXP;1
    OrganismiHordeum vulgare (Barley)
    Taxonomic identifieri4513 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

    Organism-specific databases

    GrameneiP07519.

    Subcellular locationi

    Secreted
    Note: Secreted into the endosperm.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. vacuole Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Sequence AnalysisAdd
    BLAST
    Chaini31 – 296266Serine carboxypeptidase 1 chain APRO_0000004301Add
    BLAST
    Propeptidei297 – 35155Linker peptide1 PublicationPRO_0000004302Add
    BLAST
    Chaini352 – 499148Serine carboxypeptidase 1 chain BPRO_0000004303Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi92 ↔ 388Interchain (between A and B chains)By similarity
    Glycosylationi148 – 1481N-linked (GlcNAc...)
    Disulfide bondi256 ↔ 268By similarity
    Glycosylationi262 – 2621N-linked (GlcNAc...)
    Disulfide bondi291 ↔ 355Interchain (between A and B chains)By similarity
    Glycosylationi407 – 4071N-linked (GlcNAc...)

    Post-translational modificationi

    The linker peptide is endoproteolytically excised during enzyme maturation.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Expressioni

    Developmental stagei

    After one day of germination, mainly found in the scutellum of the developing grain; barely detectable after four days, and absent from the mature grain. A lower level of expression is seen in the aleurone both during development and germination.

    Gene expression databases

    GenevestigatoriP07519.

    Interactioni

    Subunit structurei

    Carboxypeptidase I is a dimer, where each monomer is composed of two chains linked by disulfide bonds.

    Structurei

    3D structure databases

    ProteinModelPortaliP07519.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi497 – 4993Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the peptidase S10 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR001563. Peptidase_S10.
    IPR018202. Peptidase_S10_AS.
    [Graphical view]
    PANTHERiPTHR11802. PTHR11802. 1 hit.
    PfamiPF00450. Peptidase_S10. 1 hit.
    [Graphical view]
    PRINTSiPR00724. CRBOXYPTASEC.
    SUPFAMiSSF53474. SSF53474. 2 hits.
    PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
    PS00131. CARBOXYPEPT_SER_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07519-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARCRRRSGC TAGAALLLLL ALALSGGGGA APQGAEVTGL PGFDGALPSK    50
    HYAGYVTVDE GHGRNLFYYV VESERDPGKD PVVLWLNGGP GCSSFDGFVY 100
    EHGPFNFESG GSVKSLPKLH LNPYAWSKVS TMIYLDSPAG VGLSYSKNVS 150
    DYETGDLKTA TDSHTFLLKW FQLYPEFLSN PFYIAGESYA GVYVPTLSHE 200
    VVKGIQGGAK PTINFKGYMV GNGVCDTIFD GNALVPFAHG MGLISDEIYQ 250
    QASTSCHGNY WNATDGKCDT AISKIESLIS GLNIYDILEP CYHSRSIKEV 300
    NLQNSKLPQS FKDLGTTNKP FPVRTRMLGR AWPLRAPVKA GRVPSWQEVA 350
    SGVPCMSDEV ATAWLDNAAV RSAIHAQSVS AIGPWLLCTD KLYFVHDAGS 400
    MIAYHKNLTS QGYRAIIFSG DHDMCVPFTG SEAWTKSLGY GVVDSWRPWI 450
    TNGQVSGYTE GYEHGLTFAT IKGAGHTVPE YKPQEAFAFY SRWLAGSKL 499
    Length:499
    Mass (Da):54,096
    Last modified:November 1, 1997 - v4
    Checksum:i9C6674B14D9DB9BF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti102 – 1021H → P AA sequence 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09603 mRNA. Translation: CAA70816.1.
    J03897 mRNA. Translation: AAA32940.1.
    PIRiT05367. CPBHS.
    UniGeneiHv.12403.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09603 mRNA. Translation: CAA70816.1 .
    J03897 mRNA. Translation: AAA32940.1 .
    PIRi T05367. CPBHS.
    UniGenei Hv.12403.

    3D structure databases

    ProteinModelPortali P07519.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S10.004.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei P07519.

    Gene expression databases

    Genevestigatori P07519.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR001563. Peptidase_S10.
    IPR018202. Peptidase_S10_AS.
    [Graphical view ]
    PANTHERi PTHR11802. PTHR11802. 1 hit.
    Pfami PF00450. Peptidase_S10. 1 hit.
    [Graphical view ]
    PRINTSi PR00724. CRBOXYPTASEC.
    SUPFAMi SSF53474. SSF53474. 2 hits.
    PROSITEi PS00560. CARBOXYPEPT_SER_HIS. 1 hit.
    PS00131. CARBOXYPEPT_SER_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Rocher A., Lok F., Cameron-Mills V., von Wettstein D.
      Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Aleurone.
    2. "The A- and B-chains of carboxypeptidase I from germinated barley originate from a single precursor polypeptide."
      Doan N.P., Fincher G.B.
      J. Biol. Chem. 263:11106-11110(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 88-499.
    3. "Primary structure of carboxypeptidase I from malted barley."
      Soerensen S.B., Breddam K., Svendsen I.
      Carlsberg Res. Commun. 51:475-485(1986)
      Cited for: PROTEIN SEQUENCE OF 31-296 AND 352-499.

    Entry informationi

    Entry nameiCBP1_HORVU
    AccessioniPrimary (citable) accession number: P07519
    Secondary accession number(s): P07520
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 102 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3