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P07519

- CBP1_HORVU

UniProt

P07519 - CBP1_HORVU

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Protein

Serine carboxypeptidase 1

Gene

CBP1

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in the degradation of small peptides (2-5 residues) or in the degradation of storage proteins in the embryo.

Catalytic activityi

Release of a C-terminal amino acid with broad specificity.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei188 – 1881By similarity
Active sitei423 – 4231By similarity
Active sitei476 – 4761By similarity

GO - Molecular functioni

  1. serine-type carboxypeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Protein family/group databases

MEROPSiS10.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase 1 (EC:3.4.16.5)
Alternative name(s):
CP-MI
Carboxypeptidase C
Serine carboxypeptidase I
Cleaved into the following 2 chains:
Alternative name(s):
Serine carboxypeptidase I chain A
Alternative name(s):
Serine carboxypeptidase I chain B
Gene namesi
Name:CBP1
Synonyms:CXP;1
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP07519.

Subcellular locationi

Secreted
Note: Secreted into the endosperm.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 296266Serine carboxypeptidase 1 chain APRO_0000004301Add
BLAST
Propeptidei297 – 35155Linker peptide1 PublicationPRO_0000004302Add
BLAST
Chaini352 – 499148Serine carboxypeptidase 1 chain BPRO_0000004303Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi92 ↔ 388Interchain (between A and B chains)By similarity
Glycosylationi148 – 1481N-linked (GlcNAc...)
Disulfide bondi256 ↔ 268By similarity
Glycosylationi262 – 2621N-linked (GlcNAc...)
Disulfide bondi291 ↔ 355Interchain (between A and B chains)By similarity
Glycosylationi407 – 4071N-linked (GlcNAc...)

Post-translational modificationi

The linker peptide is endoproteolytically excised during enzyme maturation.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Developmental stagei

After one day of germination, mainly found in the scutellum of the developing grain; barely detectable after four days, and absent from the mature grain. A lower level of expression is seen in the aleurone both during development and germination.

Gene expression databases

GenevestigatoriP07519.

Interactioni

Subunit structurei

Carboxypeptidase I is a dimer, where each monomer is composed of two chains linked by disulfide bonds.

Structurei

3D structure databases

ProteinModelPortaliP07519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi497 – 4993Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07519-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARCRRRSGC TAGAALLLLL ALALSGGGGA APQGAEVTGL PGFDGALPSK
60 70 80 90 100
HYAGYVTVDE GHGRNLFYYV VESERDPGKD PVVLWLNGGP GCSSFDGFVY
110 120 130 140 150
EHGPFNFESG GSVKSLPKLH LNPYAWSKVS TMIYLDSPAG VGLSYSKNVS
160 170 180 190 200
DYETGDLKTA TDSHTFLLKW FQLYPEFLSN PFYIAGESYA GVYVPTLSHE
210 220 230 240 250
VVKGIQGGAK PTINFKGYMV GNGVCDTIFD GNALVPFAHG MGLISDEIYQ
260 270 280 290 300
QASTSCHGNY WNATDGKCDT AISKIESLIS GLNIYDILEP CYHSRSIKEV
310 320 330 340 350
NLQNSKLPQS FKDLGTTNKP FPVRTRMLGR AWPLRAPVKA GRVPSWQEVA
360 370 380 390 400
SGVPCMSDEV ATAWLDNAAV RSAIHAQSVS AIGPWLLCTD KLYFVHDAGS
410 420 430 440 450
MIAYHKNLTS QGYRAIIFSG DHDMCVPFTG SEAWTKSLGY GVVDSWRPWI
460 470 480 490
TNGQVSGYTE GYEHGLTFAT IKGAGHTVPE YKPQEAFAFY SRWLAGSKL
Length:499
Mass (Da):54,096
Last modified:November 1, 1997 - v4
Checksum:i9C6674B14D9DB9BF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021H → P AA sequence 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09603 mRNA. Translation: CAA70816.1.
J03897 mRNA. Translation: AAA32940.1.
PIRiT05367. CPBHS.
UniGeneiHv.12403.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09603 mRNA. Translation: CAA70816.1 .
J03897 mRNA. Translation: AAA32940.1 .
PIRi T05367. CPBHS.
UniGenei Hv.12403.

3D structure databases

ProteinModelPortali P07519.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S10.004.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P07519.

Gene expression databases

Genevestigatori P07519.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view ]
PANTHERi PTHR11802. PTHR11802. 1 hit.
Pfami PF00450. Peptidase_S10. 1 hit.
[Graphical view ]
PRINTSi PR00724. CRBOXYPTASEC.
SUPFAMi SSF53474. SSF53474. 2 hits.
PROSITEi PS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Rocher A., Lok F., Cameron-Mills V., von Wettstein D.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Aleurone.
  2. "The A- and B-chains of carboxypeptidase I from germinated barley originate from a single precursor polypeptide."
    Doan N.P., Fincher G.B.
    J. Biol. Chem. 263:11106-11110(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 88-499.
  3. "Primary structure of carboxypeptidase I from malted barley."
    Soerensen S.B., Breddam K., Svendsen I.
    Carlsberg Res. Commun. 51:475-485(1986)
    Cited for: PROTEIN SEQUENCE OF 31-296 AND 352-499.

Entry informationi

Entry nameiCBP1_HORVU
AccessioniPrimary (citable) accession number: P07519
Secondary accession number(s): P07520
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 103 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3