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Protein

Serine carboxypeptidase 1

Gene

CBP1

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the degradation of small peptides (2-5 residues) or in the degradation of storage proteins in the embryo.

Catalytic activityi

Release of a C-terminal amino acid with broad specificity.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei188By similarity1
Active sitei423By similarity1
Active sitei476By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Protein family/group databases

ESTHERihorvu-cbp1. Carboxypeptidase_S10.
MEROPSiS10.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase 1 (EC:3.4.16.5)
Alternative name(s):
CP-MI
Carboxypeptidase C
Serine carboxypeptidase I
Cleaved into the following 2 chains:
Alternative name(s):
Serine carboxypeptidase I chain A
Alternative name(s):
Serine carboxypeptidase I chain B
Gene namesi
Name:CBP1
Synonyms:CXP;1
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

  • Secreted

  • Note: Secreted into the endosperm.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000000430131 – 296Serine carboxypeptidase 1 chain AAdd BLAST266
PropeptideiPRO_0000004302297 – 351Linker peptide1 PublicationAdd BLAST55
ChainiPRO_0000004303352 – 499Serine carboxypeptidase 1 chain BAdd BLAST148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi92 ↔ 388Interchain (between A and B chains)By similarity
Glycosylationi148N-linked (GlcNAc...)1
Disulfide bondi256 ↔ 268By similarity
Glycosylationi262N-linked (GlcNAc...)1
Disulfide bondi291 ↔ 355Interchain (between A and B chains)By similarity
Glycosylationi407N-linked (GlcNAc...)1

Post-translational modificationi

The linker peptide is endoproteolytically excised during enzyme maturation.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Developmental stagei

After one day of germination, mainly found in the scutellum of the developing grain; barely detectable after four days, and absent from the mature grain. A lower level of expression is seen in the aleurone both during development and germination.

Interactioni

Subunit structurei

Carboxypeptidase I is a dimer, where each monomer is composed of two chains linked by disulfide bonds.

Protein-protein interaction databases

STRINGi4513.MLOC_55542.1.

Structurei

3D structure databases

ProteinModelPortaliP07519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi497 – 499Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1282. Eukaryota.
COG2939. LUCA.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR033124. Ser_caboxypep_his_AS.
IPR018202. Ser_caboxypep_ser_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 2 hits.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07519-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARCRRRSGC TAGAALLLLL ALALSGGGGA APQGAEVTGL PGFDGALPSK
60 70 80 90 100
HYAGYVTVDE GHGRNLFYYV VESERDPGKD PVVLWLNGGP GCSSFDGFVY
110 120 130 140 150
EHGPFNFESG GSVKSLPKLH LNPYAWSKVS TMIYLDSPAG VGLSYSKNVS
160 170 180 190 200
DYETGDLKTA TDSHTFLLKW FQLYPEFLSN PFYIAGESYA GVYVPTLSHE
210 220 230 240 250
VVKGIQGGAK PTINFKGYMV GNGVCDTIFD GNALVPFAHG MGLISDEIYQ
260 270 280 290 300
QASTSCHGNY WNATDGKCDT AISKIESLIS GLNIYDILEP CYHSRSIKEV
310 320 330 340 350
NLQNSKLPQS FKDLGTTNKP FPVRTRMLGR AWPLRAPVKA GRVPSWQEVA
360 370 380 390 400
SGVPCMSDEV ATAWLDNAAV RSAIHAQSVS AIGPWLLCTD KLYFVHDAGS
410 420 430 440 450
MIAYHKNLTS QGYRAIIFSG DHDMCVPFTG SEAWTKSLGY GVVDSWRPWI
460 470 480 490
TNGQVSGYTE GYEHGLTFAT IKGAGHTVPE YKPQEAFAFY SRWLAGSKL
Length:499
Mass (Da):54,096
Last modified:November 1, 1997 - v4
Checksum:i9C6674B14D9DB9BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102H → P AA sequence (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09603 mRNA. Translation: CAA70816.1.
J03897 mRNA. Translation: AAA32940.1.
PIRiT05367. CPBHS.
UniGeneiHv.12403.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09603 mRNA. Translation: CAA70816.1.
J03897 mRNA. Translation: AAA32940.1.
PIRiT05367. CPBHS.
UniGeneiHv.12403.

3D structure databases

ProteinModelPortaliP07519.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4513.MLOC_55542.1.

Protein family/group databases

ESTHERihorvu-cbp1. Carboxypeptidase_S10.
MEROPSiS10.004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1282. Eukaryota.
COG2939. LUCA.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR033124. Ser_caboxypep_his_AS.
IPR018202. Ser_caboxypep_ser_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 2 hits.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBP1_HORVU
AccessioniPrimary (citable) accession number: P07519
Secondary accession number(s): P07520
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: March 16, 2016
This is version 110 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.