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P07519

- CBP1_HORVU

UniProt

P07519 - CBP1_HORVU

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Protein
Serine carboxypeptidase 1
Gene
CBP1, CXP;1
Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May be involved in the degradation of small peptides (2-5 residues) or in the degradation of storage proteins in the embryo.

Catalytic activityi

Release of a C-terminal amino acid with broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei188 – 1881 By similarity
Active sitei423 – 4231 By similarity
Active sitei476 – 4761 By similarity

GO - Molecular functioni

  1. serine-type carboxypeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Protein family/group databases

MEROPSiS10.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase 1 (EC:3.4.16.5)
Alternative name(s):
CP-MI
Carboxypeptidase C
Serine carboxypeptidase I
Cleaved into the following 2 chains:
Alternative name(s):
Serine carboxypeptidase I chain A
Alternative name(s):
Serine carboxypeptidase I chain B
Gene namesi
Name:CBP1
Synonyms:CXP;1
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP07519.

Subcellular locationi

Secreted
Note: Secreted into the endosperm.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. vacuole Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030 Reviewed prediction
Add
BLAST
Chaini31 – 296266Serine carboxypeptidase 1 chain A
PRO_0000004301Add
BLAST
Propeptidei297 – 35155Linker peptide
PRO_0000004302Add
BLAST
Chaini352 – 499148Serine carboxypeptidase 1 chain B
PRO_0000004303Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi92 ↔ 388Interchain (between A and B chains) By similarity
Glycosylationi148 – 1481N-linked (GlcNAc...)
Disulfide bondi256 ↔ 268 By similarity
Glycosylationi262 – 2621N-linked (GlcNAc...)
Disulfide bondi291 ↔ 355Interchain (between A and B chains) By similarity
Glycosylationi407 – 4071N-linked (GlcNAc...)

Post-translational modificationi

The linker peptide is endoproteolytically excised during enzyme maturation.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Developmental stagei

After one day of germination, mainly found in the scutellum of the developing grain; barely detectable after four days, and absent from the mature grain. A lower level of expression is seen in the aleurone both during development and germination.

Gene expression databases

GenevestigatoriP07519.

Interactioni

Subunit structurei

Carboxypeptidase I is a dimer, where each monomer is composed of two chains linked by disulfide bonds.

Structurei

3D structure databases

ProteinModelPortaliP07519.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi497 – 4993Microbody targeting signal Reviewed prediction

Sequence similaritiesi

Belongs to the peptidase S10 family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07519-1 [UniParc]FASTAAdd to Basket

« Hide

MARCRRRSGC TAGAALLLLL ALALSGGGGA APQGAEVTGL PGFDGALPSK    50
HYAGYVTVDE GHGRNLFYYV VESERDPGKD PVVLWLNGGP GCSSFDGFVY 100
EHGPFNFESG GSVKSLPKLH LNPYAWSKVS TMIYLDSPAG VGLSYSKNVS 150
DYETGDLKTA TDSHTFLLKW FQLYPEFLSN PFYIAGESYA GVYVPTLSHE 200
VVKGIQGGAK PTINFKGYMV GNGVCDTIFD GNALVPFAHG MGLISDEIYQ 250
QASTSCHGNY WNATDGKCDT AISKIESLIS GLNIYDILEP CYHSRSIKEV 300
NLQNSKLPQS FKDLGTTNKP FPVRTRMLGR AWPLRAPVKA GRVPSWQEVA 350
SGVPCMSDEV ATAWLDNAAV RSAIHAQSVS AIGPWLLCTD KLYFVHDAGS 400
MIAYHKNLTS QGYRAIIFSG DHDMCVPFTG SEAWTKSLGY GVVDSWRPWI 450
TNGQVSGYTE GYEHGLTFAT IKGAGHTVPE YKPQEAFAFY SRWLAGSKL 499
Length:499
Mass (Da):54,096
Last modified:November 1, 1997 - v4
Checksum:i9C6674B14D9DB9BF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021H → P AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09603 mRNA. Translation: CAA70816.1.
J03897 mRNA. Translation: AAA32940.1.
PIRiT05367. CPBHS.
UniGeneiHv.12403.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09603 mRNA. Translation: CAA70816.1 .
J03897 mRNA. Translation: AAA32940.1 .
PIRi T05367. CPBHS.
UniGenei Hv.12403.

3D structure databases

ProteinModelPortali P07519.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S10.004.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P07519.

Gene expression databases

Genevestigatori P07519.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view ]
PANTHERi PTHR11802. PTHR11802. 1 hit.
Pfami PF00450. Peptidase_S10. 1 hit.
[Graphical view ]
PRINTSi PR00724. CRBOXYPTASEC.
SUPFAMi SSF53474. SSF53474. 2 hits.
PROSITEi PS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Rocher A., Lok F., Cameron-Mills V., von Wettstein D.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Aleurone.
  2. "The A- and B-chains of carboxypeptidase I from germinated barley originate from a single precursor polypeptide."
    Doan N.P., Fincher G.B.
    J. Biol. Chem. 263:11106-11110(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 88-499.
  3. "Primary structure of carboxypeptidase I from malted barley."
    Soerensen S.B., Breddam K., Svendsen I.
    Carlsberg Res. Commun. 51:475-485(1986)
    Cited for: PROTEIN SEQUENCE OF 31-296 AND 352-499.

Entry informationi

Entry nameiCBP1_HORVU
AccessioniPrimary (citable) accession number: P07519
Secondary accession number(s): P07520
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 101 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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