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Protein

Subtilisin

Gene

apr

Organism
Bacillus pumilus (Bacillus mesentericus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.

Miscellaneous

Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation.

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.

Cofactori

Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi2Calcium 11
Active sitei32By similarity1
Metal bindingi41Calcium 11
Active sitei64By similarity1
Metal bindingi75Calcium 1; via carbonyl oxygen1
Metal bindingi77Calcium 11
Metal bindingi79Calcium 1; via carbonyl oxygen1
Metal bindingi81Calcium 1; via carbonyl oxygen1
Metal bindingi169Calcium 2; via carbonyl oxygen1
Metal bindingi171Calcium 2; via carbonyl oxygen1
Metal bindingi174Calcium 2; via carbonyl oxygen1
Active sitei221By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processSporulation
LigandCalcium, Metal-binding

Protein family/group databases

MEROPSiS08.002

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin (EC:3.4.21.62)
Alternative name(s):
Alkaline mesentericopeptidase
Gene namesi
Name:apr
OrganismiBacillus pumilus (Bacillus mesentericus)
Taxonomic identifieri1408 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000764191 – 275SubtilisinAdd BLAST275

Proteomic databases

PRIDEiP07518

Interactioni

Protein-protein interaction databases

MINTiP07518

Structurei

Secondary structure

1275
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 10Combined sources5
Helixi13 – 19Combined sources7
Beta strandi27 – 33Combined sources7
Beta strandi44 – 49Combined sources6
Helixi65 – 73Combined sources9
Beta strandi76 – 80Combined sources5
Beta strandi88 – 94Combined sources7
Helixi104 – 116Combined sources13
Beta strandi120 – 124Combined sources5
Beta strandi126 – 130Combined sources5
Helixi133 – 144Combined sources12
Beta strandi148 – 152Combined sources5
Turni168 – 170Combined sources3
Beta strandi174 – 180Combined sources7
Beta strandi198 – 201Combined sources4
Beta strandi203 – 209Combined sources7
Turni210 – 212Combined sources3
Beta strandi213 – 217Combined sources5
Helixi220 – 237Combined sources18
Helixi243 – 252Combined sources10
Helixi260 – 263Combined sources4
Helixi270 – 273Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MEEX-ray2.00A1-275[»]
ProteinModelPortaliP07518
SMRiP07518
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07518

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 273Peptidase S8Add BLAST247

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Family and domain databases

CDDicd07477 Peptidases_S8_Subtilisin_subse, 1 hit
Gene3Di3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR034202 Subtilisin_Carlsberg-like
PfamiView protein in Pfam
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

P07518-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AQSVPYGISQ IKAPALHSQG YTGSNVKVAV IDSGIDSSHP DLNVRGGASF
60 70 80 90 100
VPSETNPYQD GSSHGTHVAG TIAALNNSIG VLGVAPSSAL YAVKVLDSTG
110 120 130 140 150
SGQYSWIING IEWAISNNMD VINMSLGGPT GSTALKTVVD KAVSSGIVVA
160 170 180 190 200
AAAGNEGSSG STSTVGYPAK YPSTIAVGAV NSANQRASFS SAGSELDVMA
210 220 230 240 250
PGVSIQSTLP GGTYGAYNGT SMATPHVAGA AALILSKHPT WTNAQVRDRL
260 270
ESTATYLGSS FYYGKGLINV QAAAQ
Length:275
Mass (Da):27,656
Last modified:April 1, 1988 - v1
Checksum:i33BDA897DBA4170A
GO

Sequence databases

PIRiA23624

Similar proteinsi

Entry informationi

Entry nameiSUBT_BACPU
AccessioniPrimary (citable) accession number: P07518
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 23, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

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