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Protein

Kappa-casein

Gene

CSN3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kappa-casein stabilizes micelle formation, preventing casein precipitation in milk.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei117 – 1182Cleavage; by chymosin/rennin

GO - Biological processi

  • lactation Source: UniProtKB
  • protein stabilization Source: UniProtKB
  • transmembrane transport Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Milk protein

Enzyme and pathway databases

ReactomeiREACT_268444. Miscellaneous transport and binding events.

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-casein
Gene namesi
Name:CSN3
Synonyms:CASK, CSN10, CSNK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:2446. CSN3.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: Reactome
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26949.

Protein family/group databases

Allergomei1064. Hom s 8.

Polymorphism and mutation databases

BioMutaiCSN3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Add
BLAST
Chaini21 – 182162Kappa-caseinPRO_0000004498Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi133 – 1331O-linked (GalNAc...)1 Publication
Glycosylationi143 – 1431O-linked (GalNAc...)1 Publication
Glycosylationi148 – 1481O-linked (GalNAc...)1 Publication
Glycosylationi151 – 1511O-linked (GalNAc...)1 Publication
Glycosylationi157 – 1571O-linked (GalNAc...)1 Publication
Glycosylationi167 – 1671O-linked (GalNAc...)1 Publication
Glycosylationi169 – 1691O-linked (GalNAc...)1 Publication
Glycosylationi178 – 1781O-linked (GalNAc...); partialBy similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP07498.

PTM databases

PhosphoSiteiP07498.
UniCarbKBiP07498.

Miscellaneous databases

PMAP-CutDBP07498.

Expressioni

Tissue specificityi

Mammary gland specific. Secreted in milk.

Gene expression databases

BgeeiP07498.
CleanExiHS_CASK.
HS_CSN3.
GenevisibleiP07498. HS.

Organism-specific databases

HPAiHPA035953.

Interactioni

Subunit structurei

Heteromultimers composed of alpha-s1 casein and kappa casein linked by disulfide bonds.

Binary interactionsi

WithEntry#Exp.IntActNotes
RBPMSQ930623EBI-2602175,EBI-740322

Protein-protein interaction databases

BioGridi107835. 6 interactions.
IntActiP07498. 3 interactions.
MINTiMINT-7034630.
STRINGi9606.ENSP00000304822.

Structurei

3D structure databases

ProteinModelPortaliP07498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the kappa-casein family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40632.
GeneTreeiENSGT00390000009184.
HOGENOMiHOG000111309.
HOVERGENiHBG005246.
InParanoidiP07498.
KOiK17282.
OMAiIPPKKIQ.
OrthoDBiEOG7MWH09.
PhylomeDBiP07498.
TreeFamiTF338369.

Family and domain databases

InterProiIPR000117. Casein_kappa.
[Graphical view]
PANTHERiPTHR11470. PTHR11470. 1 hit.
PfamiPF00997. Casein_kappa. 1 hit.
[Graphical view]
PIRSFiPIRSF002374. Casein_kappa. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07498-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSFLLVVNA LALTLPFLAV EVQNQKQPAC HENDERPFYQ KTAPYVPMYY
60 70 80 90 100
VPNSYPYYGT NLYQRRPAIA INNPYVPRTY YANPAVVRPH AQIPQRQYLP
110 120 130 140 150
NSHPPTVVRR PNLHPSFIAI PPKKIQDKII IPTINTIATV EPTPAPATEP
160 170 180
TVDSVVTPEA FSESIITSTP ETTTVAVTPP TA
Length:182
Mass (Da):20,305
Last modified:October 1, 1996 - v3
Checksum:i8A1609D2D5C94895
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 273QKQ → EQK AA sequence (PubMed:4018271).Curated
Sequence conflicti153 – 1531D → N AA sequence (PubMed:4018271).Curated
Sequence conflicti161 – 1611F → T AA sequence (PubMed:7460900).Curated
Sequence conflicti162 – 1621S → T AA sequence (PubMed:4018271).Curated
Sequence conflicti162 – 1621S → T AA sequence (PubMed:7460900).Curated
Sequence conflicti173 – 1731T → P AA sequence (PubMed:4018271).Curated
Sequence conflicti173 – 1731T → P AA sequence (PubMed:7460900).Curated
Sequence conflicti178 – 1814TPPT → PTTS AA sequence (PubMed:4018271).Curated
Sequence conflicti178 – 1814TPPT → PTTS AA sequence (PubMed:7460900).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti75 – 751Y → C.1 Publication
Corresponds to variant rs17850702 [ dbSNP | Ensembl ].
VAR_026338
Natural varianti110 – 1101R → L.1 Publication
Corresponds to variant rs1048152 [ dbSNP | Ensembl ].
VAR_026339
Natural varianti145 – 1451A → T.1 Publication
Corresponds to variant rs3775739 [ dbSNP | Ensembl ].
VAR_026340

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73628 mRNA. Translation: AAA59456.1.
X66417 mRNA. Translation: CAA47048.1.
U51899 Genomic DNA. Translation: AAC50772.1.
BC010935 mRNA. Translation: AAH10935.1.
CCDSiCCDS3538.1.
PIRiJC4957. KKHU.
RefSeqiNP_005203.2. NM_005212.2.
XP_011529922.1. XM_011531620.1.
XP_011529923.1. XM_011531621.1.
UniGeneiHs.54415.

Genome annotation databases

EnsembliENST00000304954; ENSP00000304822; ENSG00000171209.
GeneIDi1448.
KEGGihsa:1448.
UCSCiuc003hfe.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Protein Spotlight

Of buttons, digestion and glue - Issue 16 of November 2001

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73628 mRNA. Translation: AAA59456.1.
X66417 mRNA. Translation: CAA47048.1.
U51899 Genomic DNA. Translation: AAC50772.1.
BC010935 mRNA. Translation: AAH10935.1.
CCDSiCCDS3538.1.
PIRiJC4957. KKHU.
RefSeqiNP_005203.2. NM_005212.2.
XP_011529922.1. XM_011531620.1.
XP_011529923.1. XM_011531621.1.
UniGeneiHs.54415.

3D structure databases

ProteinModelPortaliP07498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107835. 6 interactions.
IntActiP07498. 3 interactions.
MINTiMINT-7034630.
STRINGi9606.ENSP00000304822.

Protein family/group databases

Allergomei1064. Hom s 8.

PTM databases

PhosphoSiteiP07498.
UniCarbKBiP07498.

Polymorphism and mutation databases

BioMutaiCSN3.

Proteomic databases

PRIDEiP07498.

Protocols and materials databases

DNASUi1448.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304954; ENSP00000304822; ENSG00000171209.
GeneIDi1448.
KEGGihsa:1448.
UCSCiuc003hfe.4. human.

Organism-specific databases

CTDi1448.
GeneCardsiGC04P071156.
HGNCiHGNC:2446. CSN3.
HPAiHPA035953.
MIMi601695. gene.
neXtProtiNX_P07498.
PharmGKBiPA26949.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG40632.
GeneTreeiENSGT00390000009184.
HOGENOMiHOG000111309.
HOVERGENiHBG005246.
InParanoidiP07498.
KOiK17282.
OMAiIPPKKIQ.
OrthoDBiEOG7MWH09.
PhylomeDBiP07498.
TreeFamiTF338369.

Enzyme and pathway databases

ReactomeiREACT_268444. Miscellaneous transport and binding events.

Miscellaneous databases

GeneWikiiCSN3_(gene).
GenomeRNAii1448.
NextBioi5951.
PMAP-CutDBP07498.
PROiP07498.
SOURCEiSearch...

Gene expression databases

BgeeiP07498.
CleanExiHS_CASK.
HS_CSN3.
GenevisibleiP07498. HS.

Family and domain databases

InterProiIPR000117. Casein_kappa.
[Graphical view]
PANTHERiPTHR11470. PTHR11470. 1 hit.
PfamiPF00997. Casein_kappa. 1 hit.
[Graphical view]
PIRSFiPIRSF002374. Casein_kappa. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Menon R.S., Jeffers K.F., Chang Y.F., Ham R.G.
    Submitted (JUL-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-145.
  2. "Cloning and sequencing of human kappa-casein cDNA."
    Bergstroem S., Hansson L., Hernell O., Loennerdal B., Nilsson A.K., Stroemqvist M.
    DNA Seq. 3:245-246(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Mammary gland.
  3. "Structure of the human kappa-casein gene."
    Edlund A., Johansson T., Leidvik B., Hansson L.
    Gene 174:65-69(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT LEU-110.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-75.
    Tissue: Brain.
  5. "Preparation and amino acid sequence of human kappa-casein."
    Brignon G., Chtourou A., Ribadeau-Dumas B.
    FEBS Lett. 188:48-54(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-182.
  6. "Localisation and importance of the sugar part of human casein."
    Fiat A.-M., Jolles J., Aubert J.-P., Loucheux-Lefebvre M.H., Jolles P.
    Eur. J. Biochem. 111:333-339(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 118-182, GLYCOSYLATION AT THR-133; THR-143; THR-148; THR-151; THR-157; THR-167 AND THR-169.
  7. "Structural features of a peptide corresponding to human kappa-casein residues 84-101 by 1H-nuclear magnetic resonance spectroscopy."
    Plowman J.E., Creamer L.K., Liddell M.J., Cross J.J.
    J. Dairy Res. 66:53-63(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 108-125.

Entry informationi

Entry nameiCASK_HUMAN
AccessioniPrimary (citable) accession number: P07498
Secondary accession number(s): Q13575
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.