Reviewed, UniProtKB/Swiss-Prot P07487 (G3P2_DROME)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 2
    EC=1.2.1.12
Alternative name(s):
    Glyceraldehyde-3-phosphate dehydrogenase II
      Short name=GAPDH II

Gene names

Name:	Gapdh2
ORF Names:	CG8893

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from expression pattern. Source: FlyBase oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	lipid particle Inferred from direct assay. Source: FlyBase
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from direct assay. Source: FlyBase protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VRJ9	1	EBI-110014,EBI-100603

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Glyceraldehyde-3-phosphate dehydrogenase 2		PRO_0000145522
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	148 – 150	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	208 – 209	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	149	1	Nucleophile By similarity
	Binding site	32	1	NAD By similarity
	Binding site	77	1	NAD; via carbonyl oxygen By similarity
	Binding site	179	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	231	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	313	1	NAD By similarity
	Site	176	1	Activates thiol group during catalysis By similarity
Amino acid modifications
	Modified residue	273	1	Phosphotyrosine Ref.6
	Modified residue	274	1	Phosphothreonine Ref.6
Natural variations
	Natural variant	80	1	A → V in strain: MA2. Ref.2
Experimental info
	Sequence conflict	38	1	N → K in AAA28561. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07487-1 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 5D5D274B8D3B4839	FASTA	332	35,369
	10 20 30 40 50 60 MSKIGINGFG RIGRLVLRAA IDKGANVVAV NDPFIDVNYM VYLFKFDSTH GRFKGTVAAE 70 80 90 100 110 120 GGFLVVNGQK ITVFSERDPA NINWASAGAE YIVESTGVFT TIDKASTHLK GGAKKVIISA 130 140 150 160 170 180 PSADAPMFVC GVNLDAYKPD MKVVSNASCT TNCLAPLAKV INDNFEIVEG LMTTVHATTA 190 200 210 220 230 240 TQKTVDGPSG KLWRDGRGAA QNIIPASTGA AKAVGKVIPA LNGKLTGMAF RVPTPNVSVV 250 260 270 280 290 300 DLTVRLGKGA SYDEIKAKVQ EAANGPLKGI LGYTDEEVVS TDFLSDTHSS VFDAKAGISL 310 320 330 NDKFVKLISW YDNEFGYSNR VIDLIKYMQS KD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of two unlinked Drosophila melanogaster glyceraldehyde-3-phosphate dehydrogenase genes." Tso J.Y., Sun X.-H., Wu R. J. Biol. Chem. 260:8220-8228(1985) [PubMed: 2989282] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Adaptive evolution of metabolic pathways in Drosophila." Flowers J., Sezgin E., Kumagai S., Duvernell D., Matzkin L., Schmidt P., Eanes W. Mol. Biol. Evol. 24:1347-1354(2007) [PubMed: 17379620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-80. Strain: AF8, AF9, HFL1, HFL2, HFL3, HFL4, MA2, MA7, SC1, SC2, VT1, VT2, VT3, VT4, Zh23 and Zh33.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[6]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed: 17372656] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-273 AND THR-274, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M11255 Genomic DNA. Translation: AAA28561.1. DQ864106 Genomic DNA. Translation: ABH06741.1. DQ864107 Genomic DNA. Translation: ABH06742.1. DQ864108 Genomic DNA. Translation: ABH06743.1. DQ864109 Genomic DNA. Translation: ABH06744.1. DQ864110 Genomic DNA. Translation: ABH06745.1. DQ864111 Genomic DNA. Translation: ABH06746.1. DQ864112 Genomic DNA. Translation: ABH06747.1. DQ864113 Genomic DNA. Translation: ABH06748.1. DQ864114 Genomic DNA. Translation: ABH06749.1. DQ864115 Genomic DNA. Translation: ABH06750.1. DQ864116 Genomic DNA. Translation: ABH06751.1. DQ864117 Genomic DNA. Translation: ABH06752.1. DQ864118 Genomic DNA. Translation: ABH06753.1. DQ864119 Genomic DNA. Translation: ABH06754.1. DQ864120 Genomic DNA. Translation: ABH06755.1. DQ864121 Genomic DNA. Translation: ABH06756.1. AE014298 Genomic DNA. Translation: AAF48531.1. AE014298 Genomic DNA. Translation: AAN09371.1. AY094940 mRNA. Translation: AAM11293.1.
PIR	B22366.
RefSeq	NP_525091.1. NP_542445.1.
UniGene	Dm.6789
3D structure databases
HSSP	HSSP built from PDB template 1CRW based on UniProtKB P56649.
SMR	P07487. Positions 2-332.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:17969N.
IntAct	P07487. 17 interactions.
Proteomic databases
PRIDE	P07487.
Genome annotation databases
Ensembl	FBgn0001092. Drosophila melanogaster. [Contig view]
GeneID	32545.
KEGG	dme:Dmel_CG8893.
Organism-specific databases
FlyBase	FBgn0001092. Gapdh2.
Phylogenomic databases
HOGENOM	P07487.
OMA	P07487. YLIVANE.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-002069-MON. DMEL-XXX-02:DMEL-XXX-02-002070-MON.
BRENDA	1.2.1.12. 48.
Gene expression databases
ArrayExpress	P07487.
GermOnline	CG8893. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	779058.

Entry information

Entry name

G3P2_DROME

Accession

Primary (citable) accession number: P07487
Secondary accession number(s): A5XCW0, Q541C2, Q9VXM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project