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Protein

Glyceraldehyde-3-phosphate dehydrogenase 2

Gene

Gapdh2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (Gapdh1), Glyceraldehyde-3-phosphate dehydrogenase 2 (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (CG9010)
  2. Phosphoglycerate kinase (Pgk), Phosphoglycerate kinase, Phosphoglycerate kinase (Dmel\CG9961), Phosphoglycerate kinase (Dmel\CG9961), Phosphoglycerate kinase (Pgk), Phosphoglycerate kinase (CG9961)
  3. no protein annotated in this organism
  4. Enolase (Eno)
  5. Pyruvate kinase (CG7362), Pyruvate kinase (PyK), Pyruvate kinase (Dmel\CG2964), Pyruvate kinase (Dmel\CG7069), Pyruvate kinase (CG7069), Pyruvate kinase (CG2964)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32NADBy similarity1
Binding sitei77NAD; via carbonyl oxygenBy similarity1
Active sitei149NucleophilePROSITE-ProRule annotation1
Sitei176Activates thiol group during catalysisBy similarity1
Binding sitei179Glyceraldehyde 3-phosphateBy similarity1
Binding sitei231Glyceraldehyde 3-phosphateBy similarity1
Binding sitei313NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADBy similarity2

GO - Molecular functioni

GO - Biological processi

  • glucose metabolic process Source: InterPro
  • glycolytic process Source: FlyBase

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD

Enzyme and pathway databases

ReactomeiR-DME-70171. Glycolysis.
R-DME-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 2 (EC:1.2.1.12)
Alternative name(s):
Glyceraldehyde-3-phosphate dehydrogenase II
Short name:
GAPDH II
Gene namesi
Name:Gapdh2
ORF Names:CG8893
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0001092. Gapdh2.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001455221 – 332Glyceraldehyde-3-phosphate dehydrogenase 2Add BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei273Phosphotyrosine1 Publication1
Modified residuei274Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP07487.
PRIDEiP07487.

PTM databases

iPTMnetiP07487.

Expressioni

Gene expression databases

BgeeiFBgn0001092.
ExpressionAtlasiP07487. differential.
GenevisibleiP07487. DM.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi58893. 34 interactors.
DIPiDIP-17969N.
IntActiP07487. 2 interactors.
MINTiMINT-934462.
STRINGi7227.FBpp0073922.

Structurei

3D structure databases

ProteinModelPortaliP07487.
SMRiP07487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 150Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni208 – 209Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
GeneTreeiENSGT00760000119172.
InParanoidiP07487.
KOiK00134.
OMAiFTLENMV.
OrthoDBiEOG091G0B1Y.
PhylomeDBiP07487.

Family and domain databases

InterProiView protein in InterPro
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR036291. NAD(P)-bd_dom_sf.
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiView protein in Pfam
PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiView protein in SMART
SM00846. Gp_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiView protein in PROSITE
PS00071. GAPDH. 1 hit.

Sequencei

Sequence statusi: Complete.

P07487-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIGINGFG RIGRLVLRAA IDKGANVVAV NDPFIDVNYM VYLFKFDSTH
60 70 80 90 100
GRFKGTVAAE GGFLVVNGQK ITVFSERDPA NINWASAGAE YIVESTGVFT
110 120 130 140 150
TIDKASTHLK GGAKKVIISA PSADAPMFVC GVNLDAYKPD MKVVSNASCT
160 170 180 190 200
TNCLAPLAKV INDNFEIVEG LMTTVHATTA TQKTVDGPSG KLWRDGRGAA
210 220 230 240 250
QNIIPASTGA AKAVGKVIPA LNGKLTGMAF RVPTPNVSVV DLTVRLGKGA
260 270 280 290 300
SYDEIKAKVQ EAANGPLKGI LGYTDEEVVS TDFLSDTHSS VFDAKAGISL
310 320 330
NDKFVKLISW YDNEFGYSNR VIDLIKYMQS KD
Length:332
Mass (Da):35,369
Last modified:June 1, 2001 - v2
Checksum:i5D5D274B8D3B4839
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38N → K in AAA28561 (PubMed:2989282).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti80A → V in strain: MA2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11255 Genomic DNA. Translation: AAA28561.1.
DQ864106 Genomic DNA. Translation: ABH06741.1.
DQ864107 Genomic DNA. Translation: ABH06742.1.
DQ864108 Genomic DNA. Translation: ABH06743.1.
DQ864109 Genomic DNA. Translation: ABH06744.1.
DQ864110 Genomic DNA. Translation: ABH06745.1.
DQ864111 Genomic DNA. Translation: ABH06746.1.
DQ864112 Genomic DNA. Translation: ABH06747.1.
DQ864113 Genomic DNA. Translation: ABH06748.1.
DQ864114 Genomic DNA. Translation: ABH06749.1.
DQ864115 Genomic DNA. Translation: ABH06750.1.
DQ864116 Genomic DNA. Translation: ABH06751.1.
DQ864117 Genomic DNA. Translation: ABH06752.1.
DQ864118 Genomic DNA. Translation: ABH06753.1.
DQ864119 Genomic DNA. Translation: ABH06754.1.
DQ864120 Genomic DNA. Translation: ABH06755.1.
DQ864121 Genomic DNA. Translation: ABH06756.1.
AE014298 Genomic DNA. Translation: AAF48531.1.
AY094940 mRNA. Translation: AAM11293.1.
PIRiB22366.
RefSeqiNP_001259584.1. NM_001272655.1.
NP_542445.1. NM_080714.4.
UniGeneiDm.6789.

Genome annotation databases

EnsemblMetazoaiFBtr0074112; FBpp0073922; FBgn0001092.
FBtr0332618; FBpp0304864; FBgn0001092.
GeneIDi32545.
KEGGidme:Dmel_CG8893.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiG3P2_DROME
AccessioniPrimary (citable) accession number: P07487
Secondary accession number(s): A5XCW0, Q541C2, Q9VXM7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 1, 2001
Last modified: November 22, 2017
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families