Reviewed,
UniProtKB/Swiss-Prot P07486 (G3P1_DROME)
Last modified
June 16, 2009.
Version 86.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glyceraldehyde-3-phosphate dehydrogenase 1 EC=1.2.1.12 Alternative name(s): Glyceraldehyde-3-phosphate dehydrogenase I Short name=GAPDH I | ||||||
| Gene names |
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| Organism | Drosophila melanogaster (Fruit fly) [Complete proteome] | ||||||
| Taxonomic identifier | 7227 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora |
Protein attributes
| Sequence length | 332 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. |
| Pathway | Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. |
| Subunit structure | Homotetramer. |
| Subcellular location | |
| Developmental stage | Expressed during adult stages. Ref.1 |
| Sequence similarities | Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycolysis |
| Cellular component | Cytoplasm |
| Coding sequence diversity | Polymorphism |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from expression pattern. Source: FlyBase oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | lipid particle Inferred from direct assay. Source: FlyBase |
| Molecular function | NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activityInferred from direct assay. Source: FlyBase |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 332 | 332 | Glyceraldehyde-3-phosphate dehydrogenase 1 | PRO_0000145521 | |||||
Regions | |||||||||
| Nucleotide binding | 11 – 12 | 2 | NAD By similarity | ||||||
| Region | 148 – 150 | 3 | Glyceraldehyde 3-phosphate binding By similarity | ||||||
| Region | 208 – 209 | 2 | Glyceraldehyde 3-phosphate binding By similarity | ||||||
Sites | |||||||||
| Active site | 149 | 1 | Nucleophile By similarity | ||||||
| Binding site | 32 | 1 | NAD By similarity | ||||||
| Binding site | 77 | 1 | NAD; via carbonyl oxygen By similarity | ||||||
| Binding site | 179 | 1 | Glyceraldehyde 3-phosphate By similarity | ||||||
| Binding site | 231 | 1 | Glyceraldehyde 3-phosphate By similarity | ||||||
| Binding site | 313 | 1 | NAD By similarity | ||||||
| Site | 176 | 1 | Activates thiol group during catalysis By similarity | ||||||
Natural variations | |||||||||
| Natural variant | 284 | 1 | F → L in strain: HFL23, HFL5, VT10, VT6 and HFL2. Ref.1 Ref.2 | ||||||
| Natural variant | 284 | 1 | F → V in strain: VT37, HFL15 and HFL3. Ref.1 Ref.2 | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| M11254 Genomic DNA. Translation: AAA28560.1. DQ864083 Genomic DNA. Translation: ABH06718.1. DQ864084 Genomic DNA. Translation: ABH06719.1. DQ864085 Genomic DNA. Translation: ABH06720.1. DQ864086 Genomic DNA. Translation: ABH06721.1. DQ864087 Genomic DNA. Translation: ABH06722.1. DQ864088 Genomic DNA. Translation: ABH06723.1. DQ864089 Genomic DNA. Translation: ABH06724.1. DQ864090 Genomic DNA. Translation: ABH06725.1. DQ864091 Genomic DNA. Translation: ABH06726.1. DQ864092 Genomic DNA. Translation: ABH06727.1. DQ864093 Genomic DNA. Translation: ABH06728.1. DQ864094 Genomic DNA. Translation: ABH06729.1. AE013599 Genomic DNA. Translation: AAF59192.2. AE013599 Genomic DNA. Translation: ABC66066.1. AY089643 mRNA. Translation: AAL90381.1. BT004485 mRNA. Translation: AAO42649.1. | |
| PIR | A22366. |
| RefSeq | NP_001033936.1. NP_525108.2. |
| UniGene | Dm.23224 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1DSS based on UniProtKB P56649. |
| SMR | P07486. Positions 2-332. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P07486. 5 interactions. |
Genome annotation databases | |
| Ensembl | FBgn0001091. Drosophila melanogaster. [Contig view] |
| GeneID | 35728. |
| KEGG | dme:Dmel_CG12055. |
| NMPDR | fig|7227.3.peg.3729. |
Organism-specific databases | |
| FlyBase | FBgn0001091. Gapdh1. |
Phylogenomic databases | |
| HOGENOM | P07486. |
| OMA | P07486. SSYSSIF. |
Enzyme and pathway databases | |
| BRENDA | 1.2.1.12. 48. |
Gene expression databases | |
| ArrayExpress | P07486. |
| GermOnline | CG12055. Drosophila melanogaster. |
Family and domain databases | |
| InterPro | IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view] |
| PANTHER | PTHR10836. GAP_DH. 1 hit. |
| Pfam | PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000149. GAP_DH. 1 hit. |
| PRINTS | PR00078. G3PDHDRGNASE. |
| TIGRFAMs | TIGR01534. GAPDH-I. 1 hit. |
| PROSITE | PS00071. GAPDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 794917. |
Entry information
| Entry name | G3P1_DROME | ||||||||
| Accession | Primary (citable) accession number: P07486 Secondary accession number(s): A4UZ75 Q9V318 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Drosophila annotation project | ||||||||
Relevant documents
| Drosophila Drosophila: entries, gene names and cross-references to FlyBase |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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