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Protein

Glyceraldehyde-3-phosphate dehydrogenase 1

Gene

Gapdh1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (Gapdh1), Glyceraldehyde-3-phosphate dehydrogenase 2 (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (CG9010)
  2. Phosphoglycerate kinase (Pgk)
  3. no protein annotated in this organism
  4. Enolase (Eno)
  5. Pyruvate kinase (CG7069), Pyruvate kinase (CG7069), Pyruvate kinase (CG7362), Pyruvate kinase (PyK), Pyruvate kinase (CG2964), Pyruvate kinase (CG2964)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32NADBy similarity1
Binding sitei77NAD; via carbonyl oxygenBy similarity1
Active sitei149NucleophilePROSITE-ProRule annotation1
Sitei176Activates thiol group during catalysisBy similarity1
Binding sitei179Glyceraldehyde 3-phosphateBy similarity1
Binding sitei231Glyceraldehyde 3-phosphateBy similarity1
Binding sitei313NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADBy similarity2

GO - Molecular functioni

GO - Biological processi

  • glucose metabolic process Source: InterPro
  • glycolytic process Source: FlyBase
  • myoblast fusion Source: FlyBase
  • somatic muscle development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 1 (EC:1.2.1.12)
Alternative name(s):
Glyceraldehyde-3-phosphate dehydrogenase I
Short name:
GAPDH I
Gene namesi
Name:Gapdh1
Synonyms:Gadph-1
ORF Names:CG12055
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0001091. Gapdh1.

Subcellular locationi

GO - Cellular componenti

  • lipid particle Source: FlyBase
  • M band Source: FlyBase
  • Z disc Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001455211 – 332Glyceraldehyde-3-phosphate dehydrogenase 1Add BLAST332

Proteomic databases

PaxDbiP07486.
PRIDEiP07486.

Expressioni

Developmental stagei

Expressed during adult stages.1 Publication

Gene expression databases

BgeeiFBgn0001091.
GenevisibleiP07486. DM.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi61596. 16 interactors.
IntActiP07486. 13 interactors.
MINTiMINT-1572825.
STRINGi7227.FBpp0087977.

Structurei

3D structure databases

ProteinModelPortaliP07486.
SMRiP07486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 150Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni208 – 209Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
GeneTreeiENSGT00760000119172.
InParanoidiP07486.
KOiK00134.
OMAiSTRSAHF.
OrthoDBiEOG091G0B1Y.
PhylomeDBiP07486.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIGINGFG RIGRLVLRAA IDKGASVVAV NDPFIDVNYM VYLFKFDSTH
60 70 80 90 100
GRFKGTVAAE GGFLVVNGQK ITVFSERDPA NINWASAGAE YVVESTGVFT
110 120 130 140 150
TIDKASTHLK GGAKKVIISA PSADAPMFVC GVNLDAYSPD MKVVSNASCT
160 170 180 190 200
TNCLAPLAKV INDNFEIVEG LMTTVHATTA TQKTVDGPSG KLWRDGRGAA
210 220 230 240 250
QNIIPAATGA AKAVGKVIPA LNGKLTGMAF RVPTPNVSVV DLTVRLGKGA
260 270 280 290 300
TYDEIKAKVE EASKGPLKGI LGYTDEEVVS TDFFSDTHSS VFDAKAGISL
310 320 330
NDKFVKLISW YDNEFGYSNR VIDLIKYMQS KD
Length:332
Mass (Da):35,350
Last modified:June 21, 2005 - v2
Checksum:i3FB529173DFC6E42
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti284F → L in strain: HFL23, HFL5, VT10, VT6 and HFL2. 2 Publications1
Natural varianti284F → V in strain: VT37, HFL15 and HFL3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11254 Genomic DNA. Translation: AAA28560.1.
DQ864083 Genomic DNA. Translation: ABH06718.1.
DQ864084 Genomic DNA. Translation: ABH06719.1.
DQ864085 Genomic DNA. Translation: ABH06720.1.
DQ864086 Genomic DNA. Translation: ABH06721.1.
DQ864087 Genomic DNA. Translation: ABH06722.1.
DQ864088 Genomic DNA. Translation: ABH06723.1.
DQ864089 Genomic DNA. Translation: ABH06724.1.
DQ864090 Genomic DNA. Translation: ABH06725.1.
DQ864091 Genomic DNA. Translation: ABH06726.1.
DQ864092 Genomic DNA. Translation: ABH06727.1.
DQ864093 Genomic DNA. Translation: ABH06728.1.
DQ864094 Genomic DNA. Translation: ABH06729.1.
AE013599 Genomic DNA. Translation: AAF59192.2.
AE013599 Genomic DNA. Translation: ABC66066.1.
AY089643 mRNA. Translation: AAL90381.1.
BT004485 mRNA. Translation: AAO42649.1.
PIRiA22366.
RefSeqiNP_001033936.1. NM_001038847.2.
NP_525108.2. NM_080369.3.
UniGeneiDm.23224.

Genome annotation databases

EnsemblMetazoaiFBtr0088903; FBpp0087977; FBgn0001091.
FBtr0100479; FBpp0099914; FBgn0001091.
GeneIDi35728.
KEGGidme:Dmel_CG12055.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11254 Genomic DNA. Translation: AAA28560.1.
DQ864083 Genomic DNA. Translation: ABH06718.1.
DQ864084 Genomic DNA. Translation: ABH06719.1.
DQ864085 Genomic DNA. Translation: ABH06720.1.
DQ864086 Genomic DNA. Translation: ABH06721.1.
DQ864087 Genomic DNA. Translation: ABH06722.1.
DQ864088 Genomic DNA. Translation: ABH06723.1.
DQ864089 Genomic DNA. Translation: ABH06724.1.
DQ864090 Genomic DNA. Translation: ABH06725.1.
DQ864091 Genomic DNA. Translation: ABH06726.1.
DQ864092 Genomic DNA. Translation: ABH06727.1.
DQ864093 Genomic DNA. Translation: ABH06728.1.
DQ864094 Genomic DNA. Translation: ABH06729.1.
AE013599 Genomic DNA. Translation: AAF59192.2.
AE013599 Genomic DNA. Translation: ABC66066.1.
AY089643 mRNA. Translation: AAL90381.1.
BT004485 mRNA. Translation: AAO42649.1.
PIRiA22366.
RefSeqiNP_001033936.1. NM_001038847.2.
NP_525108.2. NM_080369.3.
UniGeneiDm.23224.

3D structure databases

ProteinModelPortaliP07486.
SMRiP07486.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61596. 16 interactors.
IntActiP07486. 13 interactors.
MINTiMINT-1572825.
STRINGi7227.FBpp0087977.

Proteomic databases

PaxDbiP07486.
PRIDEiP07486.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088903; FBpp0087977; FBgn0001091.
FBtr0100479; FBpp0099914; FBgn0001091.
GeneIDi35728.
KEGGidme:Dmel_CG12055.

Organism-specific databases

CTDi35728.
FlyBaseiFBgn0001091. Gapdh1.

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
GeneTreeiENSGT00760000119172.
InParanoidiP07486.
KOiK00134.
OMAiSTRSAHF.
OrthoDBiEOG091G0B1Y.
PhylomeDBiP07486.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Miscellaneous databases

GenomeRNAii35728.
PROiP07486.

Gene expression databases

BgeeiFBgn0001091.
GenevisibleiP07486. DM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3P1_DROME
AccessioniPrimary (citable) accession number: P07486
Secondary accession number(s): A4UZ75
, A5XCT3, A5XCT7, Q8SXG8, Q9V318
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 21, 2005
Last modified: November 30, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.