P07478 (TRY2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trypsin-2
EC=3.4.21.4

Alternative name(s):
Anionic trypsinogen
Serine protease 2
Trypsin II

Gene names

Name:	PRSS2
Synonyms:	TRY2, TRYP2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In the ileum, may be involved in defensin processing, including DEFA5. Ref.3
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Expressed in Paneth cells, at the base of small intestinal crypts. Ref.3
Post-translational modification	Sulfated on tyrosine.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Sulfation Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	collagen catabolic process Inferred from direct assay PubMed 12731883. Source: UniProtKB digestion Inferred from electronic annotation. Source: UniProtKB-KW extracellular matrix disassembly Traceable author statement. Source: Reactome innate immune response Traceable author statement. Source: Reactome positive regulation of cell adhesion Traceable author statement PubMed 12731883. Source: UniProtKB positive regulation of cell growth Traceable author statement PubMed 12731883. Source: UniProtKB proteolysis Inferred from direct assay PubMed 12731883. Source: UniProtKB
Cellular_component	extracellular matrix Traceable author statement PubMed 12731883. Source: UniProtKB extracellular space Inferred from mutant phenotype PubMed 16192646. Source: UniProtKB
Molecular_function	calcium ion binding Inferred from direct assay PubMed 12709065. Source: UniProtKB serine-type endopeptidase activity Inferred from direct assay PubMed 12709065PubMed 12731883. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Ref.2
	Propeptide	16 – 23	8	Activation peptide		PRO_0000028199
	Chain	24 – 247	224	Trypsin-2		PRO_0000028200
Regions
	Domain	24 – 244	221	Peptidase S1
Sites
	Active site	63	1	Charge relay system By similarity
	Active site	107	1	Charge relay system By similarity
	Active site	200	1	Charge relay system By similarity
	Metal binding	75	1	Calcium By similarity
	Metal binding	77	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	80	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	85	1	Calcium By similarity
	Site	194	1	Required for specificity By similarity
Amino acid modifications
	Modified residue	154	1	Sulfotyrosine Probable
	Disulfide bond	30 ↔ 160		By similarity
	Disulfide bond	48 ↔ 64		By similarity
	Disulfide bond	171 ↔ 185		By similarity
	Disulfide bond	196 ↔ 220		By similarity
Natural variations
	Natural variant	117	1	A → V. Corresponds to variant rs11547028 [ dbSNP | Ensembl ].		VAR_051858

Sequences

	Sequence		Length	Mass (Da)
	P07478 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 82B0F41EB8E3D5DB	FASTA	247	26,488
	10 20 30 40 50 60 MNLLLILTFV AAAVAAPFDD DDKIVGGYIC EENSVPYQVS LNSGYHFCGG SLISEQWVVS 70 80 90 100 110 120 AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPKYN SRTLDNDILL IKLSSPAVIN 130 140 150 160 170 180 SRVSAISLPT APPAAGTESL ISGWGNTLSS GADYPDELQC LDAPVLSQAE CEASYPGKIT 190 200 210 220 230 240 NNMFCVGFLE GGKDSCQGDS GGPVVSNGEL QGIVSWGYGC AQKNRPGVYT KVYNYVDWIK DTIAANS

References

[1]	"Cloning, characterization and nucleotide sequences of two cDNAs encoding human pancreatic trypsinogens." Emi M., Nakamura Y., Ogawa M., Yamamoto T., Nishide T., Mori T., Matsubara K. Gene 41:305-310(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Immunoreactive anionic and cationic trypsin in human serum." Kimland M., Russick C., Marks W.H., Borgstroem A. Clin. Chim. Acta 184:31-46(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-49.
[3]	"Paneth cell trypsin is the processing enzyme for human defensin-5." Ghosh D., Porter E., Shen B., Lee S.K., Wilk D., Drazba J., Yadav S.P., Crabb J.W., Ganz T., Bevins C.L. Nat. Immunol. 3:583-590(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[4]	"Human cationic trypsinogen is sulfated on Tyr154." Sahin-Toth M., Kukor Z., Nemoda Z. FEBS J. 273:5044-5050(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SULFATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27602 mRNA. Translation: AAA61232.1.
IPI	IPI00815665.
PIR	B25852.
RefSeq	NP_002761.1. NM_002770.2.
UniGene	Hs.449281. Hs.726742. Hs.728780.
3D structure databases
ProteinModelPortal	P07478.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000308720.
Protein family/group databases
MEROPS	S01.258.
PTM databases
PhosphoSite	P07478.
Polymorphism databases
DMDM	136413.
Proteomic databases
PRIDE	P07478.
Protocols and materials databases
DNASU	5645.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000570608; ENSP00000459226; ENSG00000262739.
GeneID	5645.
KEGG	hsa:5645.
Organism-specific databases
CTD	5645.
GeneCards	GC07Pg41870.
HGNC	HGNC:9483. PRSS2.
MIM	601564. gene.
neXtProt	NX_P07478.
Orphanet	676. Hereditary chronic pancreatitis.
PharmGKB	PA33833.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P07478.
KO	K01312.
Enzyme and pathway databases
Reactome	REACT_118779. Extracellular matrix organization. REACT_6900. Immune System.
Gene expression databases
Bgee	P07478.
CleanEx	HS_PRSS2.
Genevestigator	P07478.
GermOnline	ENSG00000204982. Homo sapiens.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P07478.
ChEMBL	CHEMBL3159.
GenomeRNAi	5645.
NextBio	21930.
SOURCE	Search...

Entry information

Entry name

TRY2_HUMAN

Accession

Primary (citable) accession number: P07478

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 1, 2013
This is version 134 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.