Reviewed, UniProtKB/Swiss-Prot P07478 (TRY2_HUMAN)

Names and origin

Protein names

Recommended name:
    Trypsin-2
    EC=3.4.21.4
Alternative name(s):
    Trypsin II
    Anionic trypsinogen
    Serine protease 2

Gene names

Name:	PRSS2
Synonyms:	TRY2, TRYP2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Post-translational modification	Sulfated on tyrosine.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Phosphoprotein Sulfation Zymogen
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	collagen catabolic process Inferred from direct assay. Source: UniProtKB digestion Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of cell adhesion Traceable author statement. Source: UniProtKB positive regulation of cell growth Traceable author statement. Source: UniProtKB proteolysis Inferred from direct assay. Source: UniProtKB
Cellular component	extracellular matrix Traceable author statement. Source: UniProtKB extracellular space Inferred from mutant phenotype. Source: UniProtKB
Molecular function	calcium ion binding Inferred from direct assay. Source: UniProtKB protein binding Inferred from physical interaction. Source: UniProtKB serine-type endopeptidase activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Ref.2
	Propeptide	16 – 23	8	Activation peptide		PRO_0000028199
	Chain	24 – 247	224	Trypsin-2		PRO_0000028200
Regions
	Domain	24 – 244	221	Peptidase S1
Sites
	Active site	63	1	Charge relay system By similarity
	Active site	107	1	Charge relay system By similarity
	Active site	200	1	Charge relay system By similarity
	Metal binding	75	1	Calcium By similarity
	Metal binding	77	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	80	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	85	1	Calcium By similarity
	Site	194	1	Required for specificity By similarity
Amino acid modifications
	Modified residue	8	1	Phosphothreonine Ref.4
	Modified residue	154	1	Sulfotyrosine Probable
	Disulfide bond	30 ↔ 160		By similarity
	Disulfide bond	48 ↔ 64		By similarity
	Disulfide bond	171 ↔ 185		By similarity
	Disulfide bond	196 ↔ 220		By similarity
Natural variations
	Natural variant	117	1	A → V: dbSNP rs11547028.		VAR_051858

Sequences

	Sequence		Length	Mass (Da)
	P07478-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 82B0F41EB8E3D5DB	FASTA	247	26,488
	10 20 30 40 50 60 MNLLLILTFV AAAVAAPFDD DDKIVGGYIC EENSVPYQVS LNSGYHFCGG SLISEQWVVS 70 80 90 100 110 120 AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPKYN SRTLDNDILL IKLSSPAVIN 130 140 150 160 170 180 SRVSAISLPT APPAAGTESL ISGWGNTLSS GADYPDELQC LDAPVLSQAE CEASYPGKIT 190 200 210 220 230 240 NNMFCVGFLE GGKDSCQGDS GGPVVSNGEL QGIVSWGYGC AQKNRPGVYT KVYNYVDWIK DTIAANS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, characterization and nucleotide sequences of two cDNAs encoding human pancreatic trypsinogens." Emi M., Nakamura Y., Ogawa M., Yamamoto T., Nishide T., Mori T., Matsubara K. Gene 41:305-310(1986) [PubMed: 3011602] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Immunoreactive anionic and cationic trypsin in human serum." Kimland M., Russick C., Marks W.H., Borgstroem A. Clin. Chim. Acta 184:31-46(1989) [PubMed: 2598466] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-49.
[3]	"Human cationic trypsinogen is sulfated on Tyr154." Sahin-Toth M., Kukor Z., Nemoda Z. FEBS J. 273:5044-5050(2006) [PubMed: 17087724] [Abstract] Cited for: SULFATION.
[4]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-8, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M27602 mRNA. Translation: AAA61232.1.
IPI	IPI00011695.
PIR	B25852.
RefSeq	NP_002761.1.
UniGene	Hs.622865
3D structure databases
HSSP	HSSP built from PDB template 1TRN based on UniProtKB P07477.
SMR	P07478. Positions 24-247.
ModBase	Search...
Protein family/group databases
MEROPS	S01.258.
PTM databases
PhosphoSite	P07478.
Proteomic databases
PRIDE	P07478.
Genome annotation databases
Ensembl	ENSG00000204982. Homo sapiens. [Contig view]
GeneID	5645.
KEGG	hsa:5645.
UCSC	uc003wap.1. human.
Organism-specific databases
GeneCards	GC07P142167.
H-InvDB	HIX0078762.
HGNC	HGNC:9483. PRSS2.
MIM	601564. gene.
Orphanet	676. Pancreatitis, hereditary.
PharmGKB	PA33833.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P07478.
Enzyme and pathway databases
BRENDA	3.4.21.4. 247.
Gene expression databases
ArrayExpress	P07478.
Bgee	P07478.
CleanEx	HS_PRSS2.
GermOnline	ENSG00000204982. Homo sapiens.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	21930.
SOURCE	Search...

Entry information

Entry name

TRY2_HUMAN

Accession

Primary (citable) accession number: P07478

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	July 7, 2009
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)