Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Trypsin-2

Gene

PRSS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In the ileum, may be involved in defensin processing, including DEFA5.1 Publication

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63Charge relay systemBy similarity1
Metal bindingi75CalciumBy similarity1
Metal bindingi77Calcium; via carbonyl oxygenBy similarity1
Metal bindingi80Calcium; via carbonyl oxygenBy similarity1
Metal bindingi85CalciumBy similarity1
Active sitei107Charge relay systemBy similarity1
Sitei194Required for specificityBy similarity1
Active sitei200Charge relay systemBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • serine-type endopeptidase activity Source: UniProtKB
  • serine-type peptidase activity Source: Reactome

GO - Biological processi

  • collagen catabolic process Source: UniProtKB
  • digestion Source: GO_Central
  • extracellular matrix disassembly Source: Reactome
  • positive regulation of cell adhesion Source: UniProtKB
  • positive regulation of cell growth Source: UniProtKB
  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-1442490. Collagen degradation.
R-HSA-1462054. Alpha-defensins.
R-HSA-1592389. Activation of Matrix Metalloproteinases.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-6803157. Antimicrobial peptides.

Protein family/group databases

MEROPSiS01.258.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-2 (EC:3.4.21.4)
Alternative name(s):
Anionic trypsinogen
Serine protease 2
Trypsin II
Gene namesi
Name:PRSS2
Synonyms:TRY2, TRYP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:9483. PRSS2.

Subcellular locationi

GO - Cellular componenti

  • extracellular matrix Source: UniProtKB
  • extracellular region Source: UniProtKB
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi5645.
MalaCardsiPRSS2.
OpenTargetsiENSG00000275896.
ENSG00000282049.
Orphaneti676. Hereditary chronic pancreatitis.
PharmGKBiPA33833.

Chemistry databases

ChEMBLiCHEMBL3159.
DrugBankiDB02325. Isopropyl Alcohol.

Polymorphism and mutation databases

DMDMi136413.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 151 PublicationAdd BLAST15
PropeptideiPRO_000002819916 – 23Activation peptide8
ChainiPRO_000002820024 – 247Trypsin-2Add BLAST224

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 160PROSITE-ProRule annotation
Disulfide bondi48 ↔ 64PROSITE-ProRule annotation
Modified residuei154Sulfotyrosine2 Publications1
Disulfide bondi171 ↔ 185PROSITE-ProRule annotation
Disulfide bondi196 ↔ 220PROSITE-ProRule annotation

Post-translational modificationi

Sulfated on tyrosine.1 Publication
Sulfation at Tyr-154 increases selectivity towards basic versus apolar residues at the P2' position of inhibitors that bind in a substrate-like fashion. Although the increase in selectivity is relatively small, it may facilitate digestion of a broader range of dietary proteins.1 Publication

Keywords - PTMi

Disulfide bond, Sulfation, Zymogen

Proteomic databases

EPDiP07478.
PeptideAtlasiP07478.
PRIDEiP07478.
TopDownProteomicsiP07478.

PTM databases

iPTMnetiP07478.
PhosphoSitePlusiP07478.

Expressioni

Tissue specificityi

Expressed in Paneth cells, at the base of small intestinal crypts.1 Publication

Gene expression databases

BgeeiENSG00000262739.
CleanExiHS_PRSS2.
ExpressionAtlasiP07478. baseline and differential.
GenevisibleiP07478. HS.

Organism-specific databases

HPAiHPA062452.
HPA063471.

Interactioni

Protein-protein interaction databases

BioGridi111627. 11 interactors.

Chemistry databases

BindingDBiP07478.

Structurei

3D structure databases

ProteinModelPortaliP07478.
SMRiP07478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 244Peptidase S1PROSITE-ProRule annotationAdd BLAST221

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP07478.
KOiK01312.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07478-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLLLILTFV AAAVAAPFDD DDKIVGGYIC EENSVPYQVS LNSGYHFCGG
60 70 80 90 100
SLISEQWVVS AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPKYN
110 120 130 140 150
SRTLDNDILL IKLSSPAVIN SRVSAISLPT APPAAGTESL ISGWGNTLSS
160 170 180 190 200
GADYPDELQC LDAPVLSQAE CEASYPGKIT NNMFCVGFLE GGKDSCQGDS
210 220 230 240
GGPVVSNGEL QGIVSWGYGC AQKNRPGVYT KVYNYVDWIK DTIAANS
Length:247
Mass (Da):26,488
Last modified:April 1, 1988 - v1
Checksum:i82B0F41EB8E3D5DB
GO

Polymorphismi

His-153 variation is a common polymorphism in African populations with a minor allele frequency of 9.2%, it eliminates sulfation at Tyr-154, with no consequences on digestive physiology.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051858117A → V.Corresponds to variant rs11547028dbSNPEnsembl.1
Natural variantiVAR_071761153D → H Abolishes tyrosine sulfation. 1 PublicationCorresponds to variant rs1804564dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27602 mRNA. Translation: AAA61232.1.
CCDSiCCDS83236.1.
PIRiB25852.
RefSeqiNP_002761.1. NM_002770.3.
UniGeneiHs.449281.
Hs.726742.
Hs.728780.

Genome annotation databases

EnsembliENST00000539842; ENSP00000488338; ENSG00000275896.
ENST00000632112; ENSP00000487952; ENSG00000282049.
GeneIDi5645.
KEGGihsa:5645.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27602 mRNA. Translation: AAA61232.1.
CCDSiCCDS83236.1.
PIRiB25852.
RefSeqiNP_002761.1. NM_002770.3.
UniGeneiHs.449281.
Hs.726742.
Hs.728780.

3D structure databases

ProteinModelPortaliP07478.
SMRiP07478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111627. 11 interactors.

Chemistry databases

BindingDBiP07478.
ChEMBLiCHEMBL3159.
DrugBankiDB02325. Isopropyl Alcohol.

Protein family/group databases

MEROPSiS01.258.

PTM databases

iPTMnetiP07478.
PhosphoSitePlusiP07478.

Polymorphism and mutation databases

DMDMi136413.

Proteomic databases

EPDiP07478.
PeptideAtlasiP07478.
PRIDEiP07478.
TopDownProteomicsiP07478.

Protocols and materials databases

DNASUi5645.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000539842; ENSP00000488338; ENSG00000275896.
ENST00000632112; ENSP00000487952; ENSG00000282049.
GeneIDi5645.
KEGGihsa:5645.

Organism-specific databases

CTDi5645.
DisGeNETi5645.
GeneCardsiPRSS2.
HGNCiHGNC:9483. PRSS2.
HPAiHPA062452.
HPA063471.
MalaCardsiPRSS2.
MIMi601564. gene.
neXtProtiNX_P07478.
OpenTargetsiENSG00000275896.
ENSG00000282049.
Orphaneti676. Hereditary chronic pancreatitis.
PharmGKBiPA33833.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP07478.
KOiK01312.

Enzyme and pathway databases

ReactomeiR-HSA-1442490. Collagen degradation.
R-HSA-1462054. Alpha-defensins.
R-HSA-1592389. Activation of Matrix Metalloproteinases.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-6803157. Antimicrobial peptides.

Miscellaneous databases

GenomeRNAii5645.
PROiP07478.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000262739.
CleanExiHS_PRSS2.
ExpressionAtlasiP07478. baseline and differential.
GenevisibleiP07478. HS.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRY2_HUMAN
AccessioniPrimary (citable) accession number: P07478
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 30, 2016
This is version 168 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.