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P07478

- TRY2_HUMAN

UniProt

P07478 - TRY2_HUMAN

Protein

Trypsin-2

Gene

PRSS2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    In the ileum, may be involved in defensin processing, including DEFA5.1 Publication

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei63 – 631Charge relay systemBy similarity
    Metal bindingi75 – 751CalciumBy similarity
    Metal bindingi77 – 771Calcium; via carbonyl oxygenBy similarity
    Metal bindingi80 – 801Calcium; via carbonyl oxygenBy similarity
    Metal bindingi85 – 851CalciumBy similarity
    Active sitei107 – 1071Charge relay systemBy similarity
    Sitei194 – 1941Required for specificityBy similarity
    Active sitei200 – 2001Charge relay systemBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. serine-type endopeptidase activity Source: UniProtKB

    GO - Biological processi

    1. collagen catabolic process Source: UniProtKB
    2. digestion Source: UniProtKB-KW
    3. extracellular matrix disassembly Source: Reactome
    4. extracellular matrix organization Source: Reactome
    5. innate immune response Source: Reactome
    6. positive regulation of cell adhesion Source: UniProtKB
    7. positive regulation of cell growth Source: UniProtKB
    8. proteolysis Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_115574. Alpha-defensins.
    REACT_118682. Activation of Matrix Metalloproteinases.
    REACT_150401. Collagen degradation.

    Protein family/group databases

    MEROPSiS01.258.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin-2 (EC:3.4.21.4)
    Alternative name(s):
    Anionic trypsinogen
    Serine protease 2
    Trypsin II
    Gene namesi
    Name:PRSS2
    Synonyms:TRY2, TRYP2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Unplaced

    Organism-specific databases

    HGNCiHGNC:9483. PRSS2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular matrix Source: UniProtKB
    2. extracellular region Source: UniProtKB
    3. extracellular space Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    Orphaneti676. Hereditary chronic pancreatitis.
    PharmGKBiPA33833.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 15151 PublicationAdd
    BLAST
    Propeptidei16 – 238Activation peptidePRO_0000028199
    Chaini24 – 247224Trypsin-2PRO_0000028200Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi30 ↔ 160PROSITE-ProRule annotation
    Disulfide bondi48 ↔ 64PROSITE-ProRule annotation
    Modified residuei154 – 1541Sulfotyrosine1 Publication
    Disulfide bondi171 ↔ 185PROSITE-ProRule annotation
    Disulfide bondi196 ↔ 220PROSITE-ProRule annotation

    Post-translational modificationi

    Sulfated on tyrosine.1 Publication

    Keywords - PTMi

    Disulfide bond, Sulfation, Zymogen

    Proteomic databases

    MaxQBiP07478.
    PRIDEiP07478.

    PTM databases

    PhosphoSiteiP07478.

    Expressioni

    Tissue specificityi

    Expressed in Paneth cells, at the base of small intestinal crypts.1 Publication

    Gene expression databases

    BgeeiP07478.
    CleanExiHS_PRSS2.
    GenevestigatoriP07478.

    Interactioni

    Protein-protein interaction databases

    BioGridi111627. 1 interaction.
    STRINGi9606.ENSP00000308720.

    Structurei

    3D structure databases

    ProteinModelPortaliP07478.
    SMRiP07478. Positions 24-247.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 244221Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP07478.
    KOiK01312.
    OMAiHPKYSSW.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07478-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLLLILTFV AAAVAAPFDD DDKIVGGYIC EENSVPYQVS LNSGYHFCGG    50
    SLISEQWVVS AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPKYN 100
    SRTLDNDILL IKLSSPAVIN SRVSAISLPT APPAAGTESL ISGWGNTLSS 150
    GADYPDELQC LDAPVLSQAE CEASYPGKIT NNMFCVGFLE GGKDSCQGDS 200
    GGPVVSNGEL QGIVSWGYGC AQKNRPGVYT KVYNYVDWIK DTIAANS 247
    Length:247
    Mass (Da):26,488
    Last modified:April 1, 1988 - v1
    Checksum:i82B0F41EB8E3D5DB
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti117 – 1171A → V.
    Corresponds to variant rs11547028 [ dbSNP | Ensembl ].
    VAR_051858

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27602 mRNA. Translation: AAA61232.1.
    PIRiB25852.
    RefSeqiNP_002761.1. NM_002770.2.
    UniGeneiHs.449281.
    Hs.726742.
    Hs.728780.

    Genome annotation databases

    GeneIDi5645.
    KEGGihsa:5645.

    Polymorphism databases

    DMDMi136413.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27602 mRNA. Translation: AAA61232.1 .
    PIRi B25852.
    RefSeqi NP_002761.1. NM_002770.2.
    UniGenei Hs.449281.
    Hs.726742.
    Hs.728780.

    3D structure databases

    ProteinModelPortali P07478.
    SMRi P07478. Positions 24-247.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111627. 1 interaction.
    STRINGi 9606.ENSP00000308720.

    Chemistry

    BindingDBi P07478.
    ChEMBLi CHEMBL2095204.

    Protein family/group databases

    MEROPSi S01.258.

    PTM databases

    PhosphoSitei P07478.

    Polymorphism databases

    DMDMi 136413.

    Proteomic databases

    MaxQBi P07478.
    PRIDEi P07478.

    Protocols and materials databases

    DNASUi 5645.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5645.
    KEGGi hsa:5645.

    Organism-specific databases

    CTDi 5645.
    GeneCardsi GC07P9T0974.
    HGNCi HGNC:9483. PRSS2.
    MIMi 601564. gene.
    neXtProti NX_P07478.
    Orphaneti 676. Hereditary chronic pancreatitis.
    PharmGKBi PA33833.
    GenAtlasi Search...

    Phylogenomic databases

    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P07478.
    KOi K01312.
    OMAi HPKYSSW.

    Enzyme and pathway databases

    Reactomei REACT_115574. Alpha-defensins.
    REACT_118682. Activation of Matrix Metalloproteinases.
    REACT_150401. Collagen degradation.

    Miscellaneous databases

    GenomeRNAii 5645.
    NextBioi 21930.
    PROi P07478.
    SOURCEi Search...

    Gene expression databases

    Bgeei P07478.
    CleanExi HS_PRSS2.
    Genevestigatori P07478.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, characterization and nucleotide sequences of two cDNAs encoding human pancreatic trypsinogens."
      Emi M., Nakamura Y., Ogawa M., Yamamoto T., Nishide T., Mori T., Matsubara K.
      Gene 41:305-310(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Immunoreactive anionic and cationic trypsin in human serum."
      Kimland M., Russick C., Marks W.H., Borgstroem A.
      Clin. Chim. Acta 184:31-46(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 16-49.
    3. "Paneth cell trypsin is the processing enzyme for human defensin-5."
      Ghosh D., Porter E., Shen B., Lee S.K., Wilk D., Drazba J., Yadav S.P., Crabb J.W., Ganz T., Bevins C.L.
      Nat. Immunol. 3:583-590(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    4. "Human cationic trypsinogen is sulfated on Tyr154."
      Sahin-Toth M., Kukor Z., Nemoda Z.
      FEBS J. 273:5044-5050(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SULFATION.

    Entry informationi

    Entry nameiTRY2_HUMAN
    AccessioniPrimary (citable) accession number: P07478
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 146 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3