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Protein

Trypsin-2

Gene

PRSS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

In the ileum, may be involved in defensin processing, including DEFA5.1 Publication

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63Charge relay systemBy similarity1
Metal bindingi75CalciumBy similarity1
Metal bindingi77Calcium; via carbonyl oxygenBy similarity1
Metal bindingi80Calcium; via carbonyl oxygenBy similarity1
Metal bindingi85CalciumBy similarity1
Active sitei107Charge relay systemBy similarity1
Sitei194Required for specificityBy similarity1
Active sitei200Charge relay systemBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • metalloendopeptidase activity Source: Reactome
  • serine-type endopeptidase activity Source: UniProtKB
  • serine-type peptidase activity Source: Reactome

GO - Biological processi

  • antimicrobial humoral response Source: Reactome
  • collagen catabolic process Source: UniProtKB
  • digestion Source: GO_Central
  • extracellular matrix disassembly Source: Reactome
  • neutrophil degranulation Source: Reactome
  • positive regulation of cell adhesion Source: UniProtKB
  • positive regulation of cell growth Source: UniProtKB
  • proteolysis Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processDigestion
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-1442490 Collagen degradation
R-HSA-1462054 Alpha-defensins
R-HSA-1592389 Activation of Matrix Metalloproteinases
R-HSA-6798695 Neutrophil degranulation
R-HSA-6803157 Antimicrobial peptides

Protein family/group databases

MEROPSiS01.258

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-2 (EC:3.4.21.4)
Alternative name(s):
Anionic trypsinogen
Serine protease 2
Trypsin II
Gene namesi
Name:PRSS2
Synonyms:TRY2, TRYP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000275896.4
HGNCiHGNC:9483 PRSS2
MIMi601564 gene
neXtProtiNX_P07478

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi5645
MalaCardsiPRSS2
OpenTargetsiENSG00000275896
Orphaneti676 Hereditary chronic pancreatitis
PharmGKBiPA33833

Chemistry databases

ChEMBLiCHEMBL3159
DrugBankiDB04325 2-Phenylethylamine
DB04410 3-Phenylpropylamine
DB03127 Benzamidine
DB02464 Benzylamine
DB02325 Isopropyl Alcohol
DB01805 Monoisopropylphosphorylserine
DB01973 O-Benzylsulfonyl-Serine
DB03976 Phosphorylisopropane

Polymorphism and mutation databases

DMDMi136413

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 151 PublicationAdd BLAST15
PropeptideiPRO_000002819916 – 23Activation peptide8
ChainiPRO_000002820024 – 247Trypsin-2Add BLAST224

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 160PROSITE-ProRule annotation
Disulfide bondi48 ↔ 64PROSITE-ProRule annotation
Modified residuei154Sulfotyrosine2 Publications1
Disulfide bondi171 ↔ 185PROSITE-ProRule annotation
Disulfide bondi196 ↔ 220PROSITE-ProRule annotation

Post-translational modificationi

Sulfated on tyrosine.1 Publication
Sulfation at Tyr-154 increases selectivity towards basic versus apolar residues at the P2' position of inhibitors that bind in a substrate-like fashion. Although the increase in selectivity is relatively small, it may facilitate digestion of a broader range of dietary proteins.1 Publication

Keywords - PTMi

Disulfide bond, Sulfation, Zymogen

Proteomic databases

PeptideAtlasiP07478
PRIDEiP07478
TopDownProteomicsiP07478

PTM databases

iPTMnetiP07478
PhosphoSitePlusiP07478

Expressioni

Tissue specificityi

Expressed in Paneth cells, at the base of small intestinal crypts.1 Publication

Gene expression databases

BgeeiENSG00000275896
CleanExiHS_PRSS2
ExpressionAtlasiP07478 baseline and differential
GenevisibleiP07478 HS

Organism-specific databases

HPAiHPA062452
HPA063471

Interactioni

Protein-protein interaction databases

BioGridi111627, 16 interactors
IntActiP07478, 1 interactor

Chemistry databases

BindingDBiP07478

Structurei

3D structure databases

ProteinModelPortaliP07478
SMRiP07478
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 244Peptidase S1PROSITE-ProRule annotationAdd BLAST221

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00760000118862
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP07478
KOiK01312

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07478-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLLLILTFV AAAVAAPFDD DDKIVGGYIC EENSVPYQVS LNSGYHFCGG
60 70 80 90 100
SLISEQWVVS AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPKYN
110 120 130 140 150
SRTLDNDILL IKLSSPAVIN SRVSAISLPT APPAAGTESL ISGWGNTLSS
160 170 180 190 200
GADYPDELQC LDAPVLSQAE CEASYPGKIT NNMFCVGFLE GGKDSCQGDS
210 220 230 240
GGPVVSNGEL QGIVSWGYGC AQKNRPGVYT KVYNYVDWIK DTIAANS
Length:247
Mass (Da):26,488
Last modified:April 1, 1988 - v1
Checksum:i82B0F41EB8E3D5DB
GO

Polymorphismi

His-153 variation is a common polymorphism in African populations with a minor allele frequency of 9.2%, it eliminates sulfation at Tyr-154, with no consequences on digestive physiology.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051858117A → V. Corresponds to variant dbSNP:rs11547028Ensembl.1
Natural variantiVAR_071761153D → H Abolishes tyrosine sulfation. 1 PublicationCorresponds to variant dbSNP:rs1804564Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27602 mRNA Translation: AAA61232.1
CCDSiCCDS83236.1
PIRiB25852
RefSeqiNP_002761.1, NM_002770.3
UniGeneiHs.449281
Hs.726742
Hs.728780

Genome annotation databases

EnsembliENST00000539842; ENSP00000488338; ENSG00000275896
ENST00000632112; ENSP00000487952; ENSG00000282049
GeneIDi5645
KEGGihsa:5645

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTRY2_HUMAN
AccessioniPrimary (citable) accession number: P07478
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: April 25, 2018
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

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