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P07476

- INVO_HUMAN

UniProt

P07476 - INVO_HUMAN

Protein

Involucrin

Gene

IVL

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

    GO - Molecular functioni

    1. protein binding, bridging Source: UniProtKB
    2. structural molecule activity Source: UniProtKB

    GO - Biological processi

    1. isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine Source: UniProtKB
    2. keratinization Source: UniProtKB-KW
    3. keratinocyte differentiation Source: UniProtKB
    4. peptide cross-linking Source: UniProtKB
    5. response to UV-B Source: UniProtKB

    Keywords - Biological processi

    Keratinization

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Involucrin
    Gene namesi
    Name:IVL
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:6187. IVL.

    Subcellular locationi

    Cytoplasm
    Note: Constituent of the scaffolding of the cornified envelope.

    GO - Cellular componenti

    1. cornified envelope Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. extracellular vesicular exosome Source: UniProt

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29985.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 585585InvolucrinPRO_0000159736Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi79 – 791Omega-hydroxyceramide glutamate ester1 Publication
    Lipidationi90 – 901Omega-hydroxyceramide glutamate ester1 Publication
    Lipidationi118 – 1181Omega-hydroxyceramide glutamate ester1 Publication
    Lipidationi133 – 1331Omega-hydroxyceramide glutamate ester1 Publication
    Cross-linki496 – 496Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-? in other proteins)

    Post-translational modificationi

    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

    Keywords - PTMi

    Isopeptide bond, Lipoprotein

    Proteomic databases

    MaxQBiP07476.
    PaxDbiP07476.
    PRIDEiP07476.

    PTM databases

    PhosphoSiteiP07476.

    Expressioni

    Tissue specificityi

    Keratinocytes of epidermis and other stratified squamous epithelia.

    Gene expression databases

    ArrayExpressiP07476.
    BgeeiP07476.
    CleanExiHS_IVL.
    GenevestigatoriP07476.

    Organism-specific databases

    HPAiCAB002243.
    HPA055211.

    Interactioni

    Subunit structurei

    Directly or indirectly cross-linked to cornifelin (CNFN).

    Protein-protein interaction databases

    BioGridi109917. 9 interactions.
    STRINGi9606.ENSP00000357753.

    Structurei

    Secondary structure

    1
    585
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi313 – 3186
    Helixi323 – 3286
    Helixi333 – 3386
    Helixi343 – 3486
    Helixi353 – 3586

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EU0model-A312-361[»]
    DisProtiDP00221.
    ProteinModelPortaliP07476.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati153 – 162101
    Repeati163 – 172102
    Repeati173 – 182103
    Repeati183 – 192104
    Repeati193 – 202105
    Repeati203 – 212106
    Repeati213 – 222107
    Repeati223 – 232108
    Repeati233 – 242109
    Repeati243 – 2521010
    Repeati253 – 2621011
    Repeati263 – 2721012
    Repeati273 – 2821013
    Repeati283 – 2921014
    Repeati293 – 3021015
    Repeati303 – 3121016
    Repeati313 – 3221017
    Repeati323 – 3321018
    Repeati333 – 3421019
    Repeati343 – 3521020
    Repeati353 – 3621021
    Repeati363 – 3721022
    Repeati373 – 3821023
    Repeati383 – 3921024; approximate
    Repeati393 – 4021025
    Repeati403 – 4121026
    Repeati413 – 4221027
    Repeati423 – 4321028
    Repeati433 – 4421029
    Repeati443 – 4521030
    Repeati453 – 4621031
    Repeati463 – 4721032
    Repeati473 – 4821033
    Repeati483 – 4921034
    Repeati493 – 5021035
    Repeati503 – 5121036; approximate
    Repeati513 – 5221037
    Repeati523 – 5321038
    Repeati533 – 5421039; approximate

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni153 – 54239039 X 10 AA approximate tandem repeats of [LP]-[EKG]-[LHVYQEK]-[PLSQE]-[EQDV]-[QHEKRGA]-Q-[EMVQLP]-[GKLE]-[QHVNLD]Add
    BLAST

    Sequence similaritiesi

    Belongs to the involucrin family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG80441.
    HOVERGENiHBG006166.
    InParanoidiP07476.
    OMAiPEQQVGQ.
    OrthoDBiEOG747PMZ.
    PhylomeDBiP07476.
    TreeFamiTF340025.

    Family and domain databases

    InterProiIPR002360. Involucrin.
    IPR019743. Involucrin_CS.
    IPR019571. Involucrin_N.
    IPR000354. Involucrin_rpt.
    [Graphical view]
    PANTHERiPTHR13905. PTHR13905. 1 hit.
    PfamiPF00904. Involucrin. 40 hits.
    PF10583. Involucrin_N. 1 hit.
    [Graphical view]
    PROSITEiPS00795. INVOLUCRIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07476-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQQHTLPVT LSPALSQELL KTVPPPVNTH QEQMKQPTPL PPPCQKVPVE    50
    LPVEVPSKQE EKHMTAVKGL PEQECEQQQK EPQEQELQQQ HWEQHEEYQK 100
    AENPEQQLKQ EKTQRDQQLN KQLEEEKKLL DQQLDQELVK RDEQLGMKKE 150
    QLLELPEQQE GHLKHLEQQE GQLKHPEQQE GQLELPEQQE GQLELPEQQE 200
    GQLELPEQQE GQLELPEQQE GQLELPEQQE GQLELPQQQE GQLELSEQQE 250
    GQLELSEQQE GQLKHLEHQE GQLEVPEEQM GQLKYLEQQE GQLKHLDQQE 300
    KQPELPEQQM GQLKHLEQQE GQPKHLEQQE GQLEQLEEQE GQLKHLEQQE 350
    GQLEHLEHQE GQLGLPEQQV LQLKQLEKQQ GQPKHLEEEE GQLKHLVQQE 400
    GQLKHLVQQE GQLEQQERQV EHLEQQVGQL KHLEEQEGQL KHLEQQQGQL 450
    EVPEQQVGQP KNLEQEEKQL ELPEQQEGQV KHLEKQEAQL ELPEQQVGQP 500
    KHLEQQEKHL EHPEQQDGQL KHLEQQEGQL KDLEQQKGQL EQPVFAPAPG 550
    QVQDIQPALP TKGEVLLPVE HQQQKQEVQW PPKHK 585
    Length:585
    Mass (Da):68,479
    Last modified:July 28, 2009 - v2
    Checksum:i775D2AFB90F647E8
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti113 – 1131T → A.
    Corresponds to variant rs2229496 [ dbSNP | Ensembl ].
    VAR_029019
    Natural varianti166 – 1661L → P.
    Corresponds to variant rs11205133 [ dbSNP | Ensembl ].
    VAR_029020
    Natural varianti174 – 1741K → E.
    Corresponds to variant rs12035307 [ dbSNP | Ensembl ].
    VAR_029021
    Natural varianti227 – 2271E → Q.2 Publications
    Corresponds to variant rs11807064 [ dbSNP | Ensembl ].
    VAR_058411
    Natural varianti236 – 2361P → S.2 Publications
    Corresponds to variant rs17855670 [ dbSNP | Ensembl ].
    VAR_058412
    Natural varianti237 – 2371Q → E.2 Publications
    Corresponds to variant rs7520711 [ dbSNP | Ensembl ].
    VAR_029022
    Natural varianti312 – 3121Q → K.
    Corresponds to variant rs11205137 [ dbSNP | Ensembl ].
    VAR_029023
    Natural varianti480 – 4801V → L.
    Corresponds to variant rs7545520 [ dbSNP | Ensembl ].
    VAR_029024

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13903 Genomic DNA. Translation: AAA59186.1.
    AL162596 Genomic DNA. Translation: CAI19553.1.
    BC046391 mRNA. Translation: AAH46391.1.
    CCDSiCCDS1030.1.
    PIRiA24168.
    RefSeqiNP_005538.2. NM_005547.2.
    XP_006711363.1. XM_006711300.1.
    UniGeneiHs.516439.

    Genome annotation databases

    EnsembliENST00000368764; ENSP00000357753; ENSG00000163207.
    GeneIDi3713.
    KEGGihsa:3713.
    UCSCiuc001fau.3. human.

    Polymorphism databases

    DMDMi254763301.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13903 Genomic DNA. Translation: AAA59186.1 .
    AL162596 Genomic DNA. Translation: CAI19553.1 .
    BC046391 mRNA. Translation: AAH46391.1 .
    CCDSi CCDS1030.1.
    PIRi A24168.
    RefSeqi NP_005538.2. NM_005547.2.
    XP_006711363.1. XM_006711300.1.
    UniGenei Hs.516439.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EU0 model - A 312-361 [» ]
    DisProti DP00221.
    ProteinModelPortali P07476.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109917. 9 interactions.
    STRINGi 9606.ENSP00000357753.

    PTM databases

    PhosphoSitei P07476.

    Polymorphism databases

    DMDMi 254763301.

    Proteomic databases

    MaxQBi P07476.
    PaxDbi P07476.
    PRIDEi P07476.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000368764 ; ENSP00000357753 ; ENSG00000163207 .
    GeneIDi 3713.
    KEGGi hsa:3713.
    UCSCi uc001fau.3. human.

    Organism-specific databases

    CTDi 3713.
    GeneCardsi GC01P152881.
    H-InvDB HIX0023860.
    HGNCi HGNC:6187. IVL.
    HPAi CAB002243.
    HPA055211.
    MIMi 147360. gene.
    neXtProti NX_P07476.
    PharmGKBi PA29985.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG80441.
    HOVERGENi HBG006166.
    InParanoidi P07476.
    OMAi PEQQVGQ.
    OrthoDBi EOG747PMZ.
    PhylomeDBi P07476.
    TreeFami TF340025.

    Miscellaneous databases

    ChiTaRSi IVL. human.
    GeneWikii Involucrin.
    GenomeRNAii 3713.
    NextBioi 14551.
    PROi P07476.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P07476.
    Bgeei P07476.
    CleanExi HS_IVL.
    Genevestigatori P07476.

    Family and domain databases

    InterProi IPR002360. Involucrin.
    IPR019743. Involucrin_CS.
    IPR019571. Involucrin_N.
    IPR000354. Involucrin_rpt.
    [Graphical view ]
    PANTHERi PTHR13905. PTHR13905. 1 hit.
    Pfami PF00904. Involucrin. 40 hits.
    PF10583. Involucrin_N. 1 hit.
    [Graphical view ]
    PROSITEi PS00795. INVOLUCRIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and evolution of the human involucrin gene."
      Eckert R.L., Green H.
      Cell 46:583-589(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLN-227; SER-236 AND GLU-237.
    2. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLN-227; SER-236 AND GLU-237.
      Tissue: Skin.
    4. "S100A11, S100A10, annexin I, desmosomal proteins, small proline-rich proteins, plasminogen activator inhibitor-2, and involucrin are components of the cornified envelope of cultured human epidermal keratinocytes."
      Robinson N.A., Lapic S., Welter J.F., Eckert R.L.
      J. Biol. Chem. 272:12035-12046(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 281-290.
      Tissue: Keratinocyte.
    5. "Biophysical characterization of involucrin reveals a molecule ideally suited to function as an intermolecular cross-bridge of the keratinocyte cornified envelope."
      Yaffe M.B., Beegen H., Eckert R.L.
      J. Biol. Chem. 267:12233-12238(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURAL STUDIES.
    6. "The glutamine residues reactive in transglutaminase-catalyzed cross-linking of involucrin."
      Simon M., Green H.
      J. Biol. Chem. 263:18093-18098(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLN-LYS CROSS-LINK.
    7. "Ceramides are bound to structural proteins of the human foreskin epidermal cornified cell envelope."
      Marekov L.N., Steinert P.M.
      J. Biol. Chem. 273:17763-17770(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: LIPIDATION.
    8. "Identification and characterization of a novel component of the cornified envelope, cornifelin."
      Michibata H., Chiba H., Wakimoto K., Seishima M., Kawasaki S., Okubo K., Mitsui H., Torii H., Imai Y.
      Biochem. Biophys. Res. Commun. 318:803-813(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH INVOLUCRIN.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiINVO_HUMAN
    AccessioniPrimary (citable) accession number: P07476
    Secondary accession number(s): Q5T7P4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3