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P07476 (INVO_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Involucrin
Gene names
Name:IVL
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length585 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

Subunit structure

Directly or indirectly cross-linked to cornifelin (CNFN).

Subcellular location

Cytoplasm. Note: Constituent of the scaffolding of the cornified envelope.

Tissue specificity

Keratinocytes of epidermis and other stratified squamous epithelia.

Post-translational modification

Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

Sequence similarities

Belongs to the involucrin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 585585Involucrin
PRO_0000159736

Regions

Repeat153 – 162101
Repeat163 – 172102
Repeat173 – 182103
Repeat183 – 192104
Repeat193 – 202105
Repeat203 – 212106
Repeat213 – 222107
Repeat223 – 232108
Repeat233 – 242109
Repeat243 – 2521010
Repeat253 – 2621011
Repeat263 – 2721012
Repeat273 – 2821013
Repeat283 – 2921014
Repeat293 – 3021015
Repeat303 – 3121016
Repeat313 – 3221017
Repeat323 – 3321018
Repeat333 – 3421019
Repeat343 – 3521020
Repeat353 – 3621021
Repeat363 – 3721022
Repeat373 – 3821023
Repeat383 – 3921024; approximate
Repeat393 – 4021025
Repeat403 – 4121026
Repeat413 – 4221027
Repeat423 – 4321028
Repeat433 – 4421029
Repeat443 – 4521030
Repeat453 – 4621031
Repeat463 – 4721032
Repeat473 – 4821033
Repeat483 – 4921034
Repeat493 – 5021035
Repeat503 – 5121036; approximate
Repeat513 – 5221037
Repeat523 – 5321038
Repeat533 – 5421039; approximate
Region153 – 54239039 X 10 AA approximate tandem repeats of [LP]-[EKG]-[LHVYQEK]-[PLSQE]-[EQDV]-[QHEKRGA]-Q-[EMVQLP]-[GKLE]-[QHVNLD]

Amino acid modifications

Lipidation791Omega-hydroxyceramide glutamate ester Probable
Lipidation901Omega-hydroxyceramide glutamate ester Probable
Lipidation1181Omega-hydroxyceramide glutamate ester Probable
Lipidation1331Omega-hydroxyceramide glutamate ester Probable
Cross-link496Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-? in other proteins)

Natural variations

Natural variant1131T → A.
Corresponds to variant rs2229496 [ dbSNP | Ensembl ].
VAR_029019
Natural variant1661L → P.
Corresponds to variant rs11205133 [ dbSNP | Ensembl ].
VAR_029020
Natural variant1741K → E.
Corresponds to variant rs12035307 [ dbSNP | Ensembl ].
VAR_029021
Natural variant2271E → Q. Ref.1 Ref.3
Corresponds to variant rs11807064 [ dbSNP | Ensembl ].
VAR_058411
Natural variant2361P → S. Ref.1 Ref.3
Corresponds to variant rs17855670 [ dbSNP | Ensembl ].
VAR_058412
Natural variant2371Q → E. Ref.1 Ref.3
Corresponds to variant rs7520711 [ dbSNP | Ensembl ].
VAR_029022
Natural variant3121Q → K.
Corresponds to variant rs11205137 [ dbSNP | Ensembl ].
VAR_029023
Natural variant4801V → L.
Corresponds to variant rs7545520 [ dbSNP | Ensembl ].
VAR_029024

Secondary structure

........... 585
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07476 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 775D2AFB90F647E8

FASTA58568,479
        10         20         30         40         50         60 
MSQQHTLPVT LSPALSQELL KTVPPPVNTH QEQMKQPTPL PPPCQKVPVE LPVEVPSKQE 

        70         80         90        100        110        120 
EKHMTAVKGL PEQECEQQQK EPQEQELQQQ HWEQHEEYQK AENPEQQLKQ EKTQRDQQLN 

       130        140        150        160        170        180 
KQLEEEKKLL DQQLDQELVK RDEQLGMKKE QLLELPEQQE GHLKHLEQQE GQLKHPEQQE 

       190        200        210        220        230        240 
GQLELPEQQE GQLELPEQQE GQLELPEQQE GQLELPEQQE GQLELPEQQE GQLELPQQQE 

       250        260        270        280        290        300 
GQLELSEQQE GQLELSEQQE GQLKHLEHQE GQLEVPEEQM GQLKYLEQQE GQLKHLDQQE 

       310        320        330        340        350        360 
KQPELPEQQM GQLKHLEQQE GQPKHLEQQE GQLEQLEEQE GQLKHLEQQE GQLEHLEHQE 

       370        380        390        400        410        420 
GQLGLPEQQV LQLKQLEKQQ GQPKHLEEEE GQLKHLVQQE GQLKHLVQQE GQLEQQERQV 

       430        440        450        460        470        480 
EHLEQQVGQL KHLEEQEGQL KHLEQQQGQL EVPEQQVGQP KNLEQEEKQL ELPEQQEGQV 

       490        500        510        520        530        540 
KHLEKQEAQL ELPEQQVGQP KHLEQQEKHL EHPEQQDGQL KHLEQQEGQL KDLEQQKGQL 

       550        560        570        580 
EQPVFAPAPG QVQDIQPALP TKGEVLLPVE HQQQKQEVQW PPKHK 

« Hide

References

« Hide 'large scale' references
[1]"Structure and evolution of the human involucrin gene."
Eckert R.L., Green H.
Cell 46:583-589(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLN-227; SER-236 AND GLU-237.
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLN-227; SER-236 AND GLU-237.
Tissue: Skin.
[4]"S100A11, S100A10, annexin I, desmosomal proteins, small proline-rich proteins, plasminogen activator inhibitor-2, and involucrin are components of the cornified envelope of cultured human epidermal keratinocytes."
Robinson N.A., Lapic S., Welter J.F., Eckert R.L.
J. Biol. Chem. 272:12035-12046(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 281-290.
Tissue: Keratinocyte.
[5]"Biophysical characterization of involucrin reveals a molecule ideally suited to function as an intermolecular cross-bridge of the keratinocyte cornified envelope."
Yaffe M.B., Beegen H., Eckert R.L.
J. Biol. Chem. 267:12233-12238(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURAL STUDIES.
[6]"The glutamine residues reactive in transglutaminase-catalyzed cross-linking of involucrin."
Simon M., Green H.
J. Biol. Chem. 263:18093-18098(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: GLN-LYS CROSS-LINK.
[7]"Ceramides are bound to structural proteins of the human foreskin epidermal cornified cell envelope."
Marekov L.N., Steinert P.M.
J. Biol. Chem. 273:17763-17770(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: LIPIDATION.
[8]"Identification and characterization of a novel component of the cornified envelope, cornifelin."
Michibata H., Chiba H., Wakimoto K., Seishima M., Kawasaki S., Okubo K., Mitsui H., Torii H., Imai Y.
Biochem. Biophys. Res. Commun. 318:803-813(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH INVOLUCRIN.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M13903 Genomic DNA. Translation: AAA59186.1.
AL162596 Genomic DNA. Translation: CAI19553.1.
BC046391 mRNA. Translation: AAH46391.1.
PIRA24168.
RefSeqNP_005538.2. NM_005547.2.
UniGeneHs.516439.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EU0model-A312-361[»]
DisProtDP00221.
ProteinModelPortalP07476.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109917. 8 interactions.
STRING9606.ENSP00000357753.

PTM databases

PhosphoSiteP07476.

Polymorphism databases

DMDM254763301.

Proteomic databases

PaxDbP07476.
PRIDEP07476.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000368764; ENSP00000357753; ENSG00000163207.
GeneID3713.
KEGGhsa:3713.
UCSCuc001fau.3. human.

Organism-specific databases

CTD3713.
GeneCardsGC01P152881.
H-InvDBHIX0023860.
HGNCHGNC:6187. IVL.
HPACAB002243.
HPA055211.
MIM147360. gene.
neXtProtNX_P07476.
PharmGKBPA29985.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG80441.
HOVERGENHBG006166.
InParanoidP07476.
OMAPEQQVGQ.
OrthoDBEOG747PMZ.
TreeFamTF340025.

Gene expression databases

ArrayExpressP07476.
BgeeP07476.
CleanExHS_IVL.
GenevestigatorP07476.

Family and domain databases

InterProIPR002360. Involucrin.
IPR019743. Involucrin_CS.
IPR019571. Involucrin_N.
IPR000354. Involucrin_rpt.
[Graphical view]
PANTHERPTHR13905. PTHR13905. 1 hit.
PfamPF00904. Involucrin. 40 hits.
PF10583. Involucrin_N. 1 hit.
[Graphical view]
PROSITEPS00795. INVOLUCRIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIVL. human.
GeneWikiInvolucrin.
GenomeRNAi3713.
NextBio14551.
PROP07476.
SOURCESearch...

Entry information

Entry nameINVO_HUMAN
AccessionPrimary (citable) accession number: P07476
Secondary accession number(s): Q5T7P4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: July 28, 2009
Last modified: April 16, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM