Galactoside O-acetyltransferase - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P07464 (THGA_ECOLI)

Last modified June 16, 2009. Version 89. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Galactoside O-acetyltransferase
      Short name=GAT
    EC=2.3.1.18
Alternative name(s):
    Thiogalactoside acetyltransferase

Gene names

Name:	lacA
Ordered Locus Names:	b0342, JW0333

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May assist cellular detoxification by acetylating non-metabolizable pyranosides, thereby preventing their reentry into the cell.
Catalytic activity	Acetyl-CoA + a beta-D-galactoside = CoA + a 6-acetyl-beta-D-galactoside.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Post-translational modification	The N-terminus of this protein is heterogeneous because the initiator methionine is only partially cleaved.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

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Ontologies

Keywords
Biological process	Lactose biosynthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	lactose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galactoside O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 203

203

Galactoside O-acetyltransferase

PRO_0000068696

Secondary structure

203

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P07464-1 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 31C7FEA0B0150D70
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FASTA

203

22,799

        10         20         30         40         50         60 
MNMPMTERIR AGKLFTDMCE GLPEKRLRGK TLMYEFNHSH PSEVEKRESL IKEMFATVGE 

        70         80         90        100        110        120 
NAWVEPPVYF SYGSNIHIGR NFYANFNLTI VDDYTVTIGD NVLIAPNVTL SVTGHPVHHE 

       130        140        150        160        170        180 
LRKNGEMYSF PITIGNNVWI GSHVVINPGV TIGDNSVIGA GSIVTKDIPP NVVAAGVPCR 

       190        200 
VIREINDRDK HYYFKDYKVE SSV

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The amino acid sequence of thiogalactoside transacetylase of Escherichia coli." Fowler A.V., Hediger M.A., Musso R.E., Zabin I. Biochimie 67:101-108(1985) [PubMed: 3922433] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region." Hediger M.A., Johnson D.F., Nierlich D.P., Zabin I. Proc. Natl. Acad. Sci. U.S.A. 82:6414-6418(1985) [PubMed: 3901000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Sequence of the lactose permease gene." Buechel D.E., Gronenborn B., Mueller-Hill B. Nature 283:541-545(1980) [PubMed: 6444453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26.
[7]	"Structure of the lac operon galactoside acetyltransferase." Wang X.G., Olsen L.R., Roderick S.L. Structure 10:581-588(2002) [PubMed: 11937062] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS).

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Cross-references

Sequence databases

J01636 Genomic DNA. Translation: AAA24055.1.
X51872 Genomic DNA. Translation: CAA36162.1.
U73857 Genomic DNA. Translation: AAB18066.1.
U00096 Genomic DNA. Translation: AAC73445.1.
AP009048 Genomic DNA. Translation: BAE76124.1.
V00295 Genomic DNA. Translation: CAA23572.1.

PIR

XXECTG. A94061.

RefSeq

AP_000994.1.
NP_414876.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KQA	X-ray	3.20	A/B/C	1-203	[»]
1KRR	X-ray	2.50	A/B/C	1-203	[»]
1KRU	X-ray	2.80	A/B/C	1-203	[»]
1KRV	X-ray	2.80	A/B/C	1-203	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:10078N.

Genome annotation databases

GeneID

945674.

GenomeReviews

Gene locus JW0333 in contig AP009048_GR.
Gene locus b0342 in contig U00096_GR.

KEGG

ecj:JW0333.
eco:b0342.

Organism-specific databases

EchoBASE

EB0519.

EcoGene

EG10524. lacA.

CMR

Search...

Phylogenomic databases

HOGENOM

P07464.

OMA

P07464. ADVHIEA.

Enzyme and pathway databases

BioCyc

EcoCyc:GALACTOACETYLTRAN-MON.
MetaCyc:GALACTOACETYLTRAN-MON.

Family and domain databases

InterPro

IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
[Graphical view]

Pfam

PF00132. Hexapep. 3 hits.
[Graphical view]

PROSITE

PS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

THGA_ECOLI

Accession

Primary (citable) accession number: P07464
Secondary accession number(s): P77862, Q2MC82

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	June 16, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families