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Protein

Galactoside O-acetyltransferase

Gene

lacA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May assist cellular detoxification by acetylating non-metabolizable pyranosides, thereby preventing their reentry into the cell.

Catalytic activityi

Acetyl-CoA + a beta-D-galactoside = CoA + a 6-acetyl-beta-D-galactoside.

GO - Molecular functioni

  • galactoside O-acetyltransferase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLactose biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:GALACTOACETYLTRAN-MONOMER
MetaCyc:GALACTOACETYLTRAN-MONOMER
BRENDAi2.3.1.18 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Galactoside O-acetyltransferase (EC:2.3.1.18)
Short name:
GAT
Alternative name(s):
Thiogalactoside acetyltransferase
Gene namesi
Name:lacA
Ordered Locus Names:b0342, JW0333
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10524 lacA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB02632 4-nitrophenyl-beta-D-galactoside
DB01992 Coenzyme A
DB01862 Isopropyl beta-D-thiogalactopyranoside

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000686961 – 203Galactoside O-acetyltransferaseAdd BLAST203

Post-translational modificationi

The N-terminus of this protein is heterogeneous because the initiator methionine is only partially cleaved.

Proteomic databases

PaxDbiP07464
PRIDEiP07464

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4263184, 12 interactors
DIPiDIP-10078N
IntActiP07464, 4 interactors
STRINGi316385.ECDH10B_1355

Structurei

Secondary structure

1203
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 11Combined sources7
Helixi22 – 37Combined sources16
Helixi44 – 54Combined sources11
Beta strandi55 – 57Combined sources3
Beta strandi68 – 71Combined sources4
Beta strandi76 – 78Combined sources3
Beta strandi88 – 91Combined sources4
Beta strandi96 – 98Combined sources3
Beta strandi109 – 114Combined sources6
Turni119 – 121Combined sources3
Beta strandi127 – 129Combined sources3
Beta strandi132 – 134Combined sources3
Beta strandi172 – 175Combined sources4
Turni176 – 179Combined sources4
Beta strandi180 – 184Combined sources5
Helixi187 – 189Combined sources3
Beta strandi192 – 194Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KQAX-ray3.20A/B/C1-203[»]
1KRRX-ray2.50A/B/C1-203[»]
1KRUX-ray2.80A/B/C1-203[»]
1KRVX-ray2.80A/B/C1-203[»]
ProteinModelPortaliP07464
SMRiP07464
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07464

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108UW5 Bacteria
COG0110 LUCA
HOGENOMiHOG000049435
InParanoidiP07464
KOiK00633
OMAiPLRANWG
PhylomeDBiP07464

Family and domain databases

InterProiView protein in InterPro
IPR001451 Hexapep
IPR018357 Hexapep_transf_CS
IPR024688 Mac_dom
IPR011004 Trimer_LpxA-like_sf
PfamiView protein in Pfam
PF00132 Hexapep, 1 hit
PF14602 Hexapep_2, 1 hit
PF12464 Mac, 1 hit
SMARTiView protein in SMART
SM01266 Mac, 1 hit
SUPFAMiSSF51161 SSF51161, 1 hit
PROSITEiView protein in PROSITE
PS00101 HEXAPEP_TRANSFERASES, 1 hit

Sequencei

Sequence statusi: Complete.

P07464-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMPMTERIR AGKLFTDMCE GLPEKRLRGK TLMYEFNHSH PSEVEKRESL
60 70 80 90 100
IKEMFATVGE NAWVEPPVYF SYGSNIHIGR NFYANFNLTI VDDYTVTIGD
110 120 130 140 150
NVLIAPNVTL SVTGHPVHHE LRKNGEMYSF PITIGNNVWI GSHVVINPGV
160 170 180 190 200
TIGDNSVIGA GSIVTKDIPP NVVAAGVPCR VIREINDRDK HYYFKDYKVE

SSV
Length:203
Mass (Da):22,799
Last modified:April 1, 1988 - v1
Checksum:i31C7FEA0B0150D70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01636 Genomic DNA Translation: AAA24055.1
X51872 Genomic DNA Translation: CAA36162.1
U73857 Genomic DNA Translation: AAB18066.1
U00096 Genomic DNA Translation: AAC73445.1
AP009048 Genomic DNA Translation: BAE76124.1
V00295 Genomic DNA Translation: CAA23572.1
PIRiA94061 XXECTG
RefSeqiNP_414876.1, NC_000913.3
WP_001335915.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73445; AAC73445; b0342
BAE76124; BAE76124; BAE76124
GeneIDi945674
KEGGiecj:JW0333
eco:b0342
PATRICifig|511145.12.peg.350

Similar proteinsi

Entry informationi

Entry nameiTHGA_ECOLI
AccessioniPrimary (citable) accession number: P07464
Secondary accession number(s): P77862, Q2MC82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: March 28, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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