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Protein

Galactoside O-acetyltransferase

Gene

lacA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May assist cellular detoxification by acetylating non-metabolizable pyranosides, thereby preventing their reentry into the cell.

Catalytic activityi

Acetyl-CoA + a beta-D-galactoside = CoA + a 6-acetyl-beta-D-galactoside.

GO - Molecular functioni

  • galactoside O-acetyltransferase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lactose biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:GALACTOACETYLTRAN-MONOMER.
ECOL316407:JW0333-MONOMER.
MetaCyc:GALACTOACETYLTRAN-MONOMER.
BRENDAi2.3.1.18. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactoside O-acetyltransferase (EC:2.3.1.18)
Short name:
GAT
Alternative name(s):
Thiogalactoside acetyltransferase
Gene namesi
Name:lacA
Ordered Locus Names:b0342, JW0333
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10524. lacA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000686961 – 203Galactoside O-acetyltransferaseAdd BLAST203

Post-translational modificationi

The N-terminus of this protein is heterogeneous because the initiator methionine is only partially cleaved.

Proteomic databases

PaxDbiP07464.
PRIDEiP07464.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4263184. 12 interactors.
DIPiDIP-10078N.
IntActiP07464. 4 interactors.
STRINGi511145.b0342.

Structurei

Secondary structure

1203
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 11Combined sources7
Helixi22 – 37Combined sources16
Helixi44 – 54Combined sources11
Beta strandi55 – 57Combined sources3
Beta strandi68 – 71Combined sources4
Beta strandi76 – 78Combined sources3
Beta strandi88 – 91Combined sources4
Beta strandi96 – 98Combined sources3
Beta strandi109 – 114Combined sources6
Turni119 – 121Combined sources3
Beta strandi127 – 129Combined sources3
Beta strandi132 – 134Combined sources3
Beta strandi172 – 175Combined sources4
Turni176 – 179Combined sources4
Beta strandi180 – 184Combined sources5
Helixi187 – 189Combined sources3
Beta strandi192 – 194Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KQAX-ray3.20A/B/C1-203[»]
1KRRX-ray2.50A/B/C1-203[»]
1KRUX-ray2.80A/B/C1-203[»]
1KRVX-ray2.80A/B/C1-203[»]
ProteinModelPortaliP07464.
SMRiP07464.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07464.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108UW5. Bacteria.
COG0110. LUCA.
HOGENOMiHOG000049435.
InParanoidiP07464.
KOiK00633.
OMAiHYYYKDY.
PhylomeDBiP07464.

Family and domain databases

InterProiIPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR024688. Mac_dom.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
PF14602. Hexapep_2. 1 hit.
PF12464. Mac. 1 hit.
[Graphical view]
SMARTiSM01266. Mac. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07464-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMPMTERIR AGKLFTDMCE GLPEKRLRGK TLMYEFNHSH PSEVEKRESL
60 70 80 90 100
IKEMFATVGE NAWVEPPVYF SYGSNIHIGR NFYANFNLTI VDDYTVTIGD
110 120 130 140 150
NVLIAPNVTL SVTGHPVHHE LRKNGEMYSF PITIGNNVWI GSHVVINPGV
160 170 180 190 200
TIGDNSVIGA GSIVTKDIPP NVVAAGVPCR VIREINDRDK HYYFKDYKVE

SSV
Length:203
Mass (Da):22,799
Last modified:April 1, 1988 - v1
Checksum:i31C7FEA0B0150D70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01636 Genomic DNA. Translation: AAA24055.1.
X51872 Genomic DNA. Translation: CAA36162.1.
U73857 Genomic DNA. Translation: AAB18066.1.
U00096 Genomic DNA. Translation: AAC73445.1.
AP009048 Genomic DNA. Translation: BAE76124.1.
V00295 Genomic DNA. Translation: CAA23572.1.
PIRiA94061. XXECTG.
RefSeqiNP_414876.1. NC_000913.3.
WP_001335915.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73445; AAC73445; b0342.
BAE76124; BAE76124; BAE76124.
GeneIDi945674.
KEGGiecj:JW0333.
eco:b0342.
PATRICi32115817. VBIEscCol129921_0350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01636 Genomic DNA. Translation: AAA24055.1.
X51872 Genomic DNA. Translation: CAA36162.1.
U73857 Genomic DNA. Translation: AAB18066.1.
U00096 Genomic DNA. Translation: AAC73445.1.
AP009048 Genomic DNA. Translation: BAE76124.1.
V00295 Genomic DNA. Translation: CAA23572.1.
PIRiA94061. XXECTG.
RefSeqiNP_414876.1. NC_000913.3.
WP_001335915.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KQAX-ray3.20A/B/C1-203[»]
1KRRX-ray2.50A/B/C1-203[»]
1KRUX-ray2.80A/B/C1-203[»]
1KRVX-ray2.80A/B/C1-203[»]
ProteinModelPortaliP07464.
SMRiP07464.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263184. 12 interactors.
DIPiDIP-10078N.
IntActiP07464. 4 interactors.
STRINGi511145.b0342.

Proteomic databases

PaxDbiP07464.
PRIDEiP07464.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73445; AAC73445; b0342.
BAE76124; BAE76124; BAE76124.
GeneIDi945674.
KEGGiecj:JW0333.
eco:b0342.
PATRICi32115817. VBIEscCol129921_0350.

Organism-specific databases

EchoBASEiEB0519.
EcoGeneiEG10524. lacA.

Phylogenomic databases

eggNOGiENOG4108UW5. Bacteria.
COG0110. LUCA.
HOGENOMiHOG000049435.
InParanoidiP07464.
KOiK00633.
OMAiHYYYKDY.
PhylomeDBiP07464.

Enzyme and pathway databases

BioCyciEcoCyc:GALACTOACETYLTRAN-MONOMER.
ECOL316407:JW0333-MONOMER.
MetaCyc:GALACTOACETYLTRAN-MONOMER.
BRENDAi2.3.1.18. 2026.

Miscellaneous databases

EvolutionaryTraceiP07464.
PROiP07464.

Family and domain databases

InterProiIPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR024688. Mac_dom.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
PF14602. Hexapep_2. 1 hit.
PF12464. Mac. 1 hit.
[Graphical view]
SMARTiSM01266. Mac. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHGA_ECOLI
AccessioniPrimary (citable) accession number: P07464
Secondary accession number(s): P77862, Q2MC82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.