P07464 (THGA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactoside O-acetyltransferase
Short name=GAT
EC=2.3.1.18

Alternative name(s):
Thiogalactoside acetyltransferase

Gene names

Name:	lacA
Ordered Locus Names:	b0342, JW0333

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May assist cellular detoxification by acetylating non-metabolizable pyranosides, thereby preventing their reentry into the cell.
Catalytic activity	Acetyl-CoA + a beta-D-galactoside = CoA + a 6-acetyl-beta-D-galactoside.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Post-translational modification	The N-terminus of this protein is heterogeneous because the initiator methionine is only partially cleaved.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Lactose biosynthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	lactose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	galactoside O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 203

203

Galactoside O-acetyltransferase

PRO_0000068696

Secondary structure

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203

Helix Strand Turn

Details...

Helix

5 – 11

7

Helix

22 – 37

16

Helix

44 – 54

11

Beta strand

55 – 57

3

Beta strand

68 – 71

4

Beta strand

76 – 78

3

Beta strand

88 – 91

4

Beta strand

96 – 98

3

Beta strand

109 – 114

6

Turn

119 – 121

3

Beta strand

127 – 129

3

Beta strand

132 – 134

3

Beta strand

172 – 175

4

Turn

176 – 179

4

Beta strand

180 – 184

5

Helix

187 – 189

3

Beta strand

192 – 194

3

Sequences

	Sequence		Length	Mass (Da)
	P07464 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 31C7FEA0B0150D70	FASTA	203	22,799
	10 20 30 40 50 60 MNMPMTERIR AGKLFTDMCE GLPEKRLRGK TLMYEFNHSH PSEVEKRESL IKEMFATVGE 70 80 90 100 110 120 NAWVEPPVYF SYGSNIHIGR NFYANFNLTI VDDYTVTIGD NVLIAPNVTL SVTGHPVHHE 130 140 150 160 170 180 LRKNGEMYSF PITIGNNVWI GSHVVINPGV TIGDNSVIGA GSIVTKDIPP NVVAAGVPCR 190 200 VIREINDRDK HYYFKDYKVE SSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The amino acid sequence of thiogalactoside transacetylase of Escherichia coli." Fowler A.V., Hediger M.A., Musso R.E., Zabin I. Biochimie 67:101-108(1985) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region." Hediger M.A., Johnson D.F., Nierlich D.P., Zabin I. Proc. Natl. Acad. Sci. U.S.A. 82:6414-6418(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Sequence of the lactose permease gene." Buechel D.E., Gronenborn B., Mueller-Hill B. Nature 283:541-545(1980) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26.
[7]	"Structure of the lac operon galactoside acetyltransferase." Wang X.G., Olsen L.R., Roderick S.L. Structure 10:581-588(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01636 Genomic DNA. Translation: AAA24055.1.
X51872 Genomic DNA. Translation: CAA36162.1.
U73857 Genomic DNA. Translation: AAB18066.1.
U00096 Genomic DNA. Translation: AAC73445.1.
AP009048 Genomic DNA. Translation: BAE76124.1.
V00295 Genomic DNA. Translation: CAA23572.1.

PIR

XXECTG. A94061.

RefSeq

NP_414876.1. NC_000913.2.
YP_488636.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KQA	X-ray	3.20	A/B/C	1-203	[»]
1KRR	X-ray	2.50	A/B/C	1-203	[»]
1KRU	X-ray	2.80	A/B/C	1-203	[»]
1KRV	X-ray	2.80	A/B/C	1-203	[»]

ProteinModelPortal

P07464.

SMR

P07464. Positions 2-202.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10078N.

IntAct

P07464. 4 interactions.

STRING

511145.b0342.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73445; AAC73445; b0342.
BAE76124; BAE76124; BAE76124.

GeneID

12934190.
945674.

KEGG

ecj:Y75_p0331.
eco:b0342.

PATRIC

32115817. VBIEscCol129921_0350.

Organism-specific databases

EchoBASE

EB0519.

EcoGene

EG10524. lacA.

Phylogenomic databases

eggNOG

COG0110.

HOGENOM

HOG000049435.

KO

K00633.

OMA

CYIEPPL.

ProtClustDB

PRK09527.

Enzyme and pathway databases

BioCyc

EcoCyc:GALACTOACETYLTRAN-MONOMER.
ECOL316407:JW0333-MONOMER.
MetaCyc:GALACTOACETYLTRAN-MONOMER.

Gene expression databases

Genevestigator

P07464.

Family and domain databases

InterPro

IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR024688. Maltose/galactoside_AcTrfase.
IPR011004. Trimer_LpxA-like.
[Graphical view]

Pfam

PF00132. Hexapep. 1 hit.
PF12464. Mac. 1 hit.
[Graphical view]

SUPFAM

SSF51161. Trimer_LpxA_like. 1 hit.

PROSITE

PS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P07464.

Entry information

Entry name

THGA_ECOLI

Accession

Primary (citable) accession number: P07464
Secondary accession number(s): P77862, Q2MC82

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 1, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program