Galactoside O-acetyltransferase - Escherichia coli (strain K12)

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P07464 (THGA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Galactoside O-acetyltransferase
Short name=GAT
EC=2.3.1.18

Alternative name(s):
Thiogalactoside acetyltransferase

Gene names

Name:	lacA
Ordered Locus Names:	b0342, JW0333

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May assist cellular detoxification by acetylating non-metabolizable pyranosides, thereby preventing their reentry into the cell.
Catalytic activity	Acetyl-CoA + a beta-D-galactoside = CoA + a 6-acetyl-beta-D-galactoside.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Post-translational modification	The N-terminus of this protein is heterogeneous because the initiator methionine is only partially cleaved.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Lactose biosynthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	lactose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galactoside O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 203

203

Galactoside O-acetyltransferase

PRO_0000068696

Secondary structure

203

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P07464 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 31C7FEA0B0150D70
Show »

FASTA

203

22,799

        10         20         30         40         50         60 
MNMPMTERIR AGKLFTDMCE GLPEKRLRGK TLMYEFNHSH PSEVEKRESL IKEMFATVGE 

        70         80         90        100        110        120 
NAWVEPPVYF SYGSNIHIGR NFYANFNLTI VDDYTVTIGD NVLIAPNVTL SVTGHPVHHE 

       130        140        150        160        170        180 
LRKNGEMYSF PITIGNNVWI GSHVVINPGV TIGDNSVIGA GSIVTKDIPP NVVAAGVPCR 

       190        200 
VIREINDRDK HYYFKDYKVE SSV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The amino acid sequence of thiogalactoside transacetylase of Escherichia coli." Fowler A.V., Hediger M.A., Musso R.E., Zabin I. Biochimie 67:101-108(1985) [PubMed: 3922433] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region." Hediger M.A., Johnson D.F., Nierlich D.P., Zabin I. Proc. Natl. Acad. Sci. U.S.A. 82:6414-6418(1985) [PubMed: 3901000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Sequence of the lactose permease gene." Buechel D.E., Gronenborn B., Mueller-Hill B. Nature 283:541-545(1980) [PubMed: 6444453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26.
[7]	"Structure of the lac operon galactoside acetyltransferase." Wang X.G., Olsen L.R., Roderick S.L. Structure 10:581-588(2002) [PubMed: 11937062] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01636 Genomic DNA. Translation: AAA24055.1.
X51872 Genomic DNA. Translation: CAA36162.1.
U73857 Genomic DNA. Translation: AAB18066.1.
U00096 Genomic DNA. Translation: AAC73445.1.
AP009048 Genomic DNA. Translation: BAE76124.1.
V00295 Genomic DNA. Translation: CAA23572.1.

PIR

XXECTG. A94061.

RefSeq

NP_414876.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KQA	X-ray	3.20	A/B/C	1-203	[»]
1KRR	X-ray	2.50	A/B/C	1-203	[»]
1KRU	X-ray	2.80	A/B/C	1-203	[»]
1KRV	X-ray	2.80	A/B/C	1-203	[»]

ProteinModelPortal

P07464.

SMR

P07464. Positions 2-202.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10078N.

IntAct

P07464. 4 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004023; EBESCP00000004023; EBESCG00000003288.
EBESCT00000004024; EBESCP00000004024; EBESCG00000003288.
EBESCT00000004025; EBESCP00000004025; EBESCG00000003288.
EBESCT00000004026; EBESCP00000004026; EBESCG00000003288.
EBESCT00000017191; EBESCP00000016482; EBESCG00000016250.

GeneID

945674.

GenomeReviews

Gene locus JW0333 in contig AP009048_GR.
Gene locus b0342 in contig U00096_GR.

KEGG

ecj:JW0333.
eco:b0342.

PATRIC

32115817. VBIEscCol129921_0350.

Organism-specific databases

EchoBASE

EB0519.

EcoGene

EG10524. lacA.

Phylogenomic databases

eggNOG

COG0110.

GeneTree

EBGT00050000009072.

HOGENOM

HBG699746.

OMA

PMTERIK.

PhylomeDB

P07464.

ProtClustDB

PRK09527.

Enzyme and pathway databases

BioCyc

EcoCyc:GALACTOACETYLTRAN-MONOMER.
MetaCyc:GALACTOACETYLTRAN-MONOMER.

Gene expression databases

Genevestigator

P07464.

Family and domain databases

InterPro

IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR024688. Maltose/galactoside_AcTrfase.
IPR011004. Trimer_LpxA-like.
[Graphical view]

K00633.

Pfam

PF00132. Hexapep. 1 hit.
PF12464. Mac. 1 hit.
[Graphical view]

SUPFAM

SSF51161. Trimer_LpxA_like. 1 hit.

PROSITE

PS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

THGA_ECOLI

Accession

Primary (citable) accession number: P07464
Secondary accession number(s): P77862, Q2MC82

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	January 25, 2012
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents