Reviewed,
UniProtKB/Swiss-Prot P07464 (THGA_ECOLI)
Last modified
June 16, 2009.
Version 89.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Galactoside O-acetyltransferase Short name=GAT EC=2.3.1.18 Alternative name(s): Thiogalactoside acetyltransferase | ||||
| Gene names |
| ||||
| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 203 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | May assist cellular detoxification by acetylating non-metabolizable pyranosides, thereby preventing their reentry into the cell. |
| Catalytic activity | Acetyl-CoA + a beta-D-galactoside = CoA + a 6-acetyl-beta-D-galactoside. |
| Subunit structure | Homodimer. |
| Subcellular location | |
| Post-translational modification | The N-terminus of this protein is heterogeneous because the initiator methionine is only partially cleaved. |
| Sequence similarities | Belongs to the transferase hexapeptide repeat family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lactose biosynthesis |
| Cellular component | Cytoplasm |
| Domain | Repeat |
| Molecular function | Acyltransferase Transferase |
| Technical term | 3D-structure Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | lactose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | galactoside O-acetyltransferase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 203 | 203 | Galactoside O-acetyltransferase | PRO_0000068696 | ||||||||||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||||||||||
| Helix | 5 – 11 | 7 | ||||||||||||||||||||||||||||||||||||
| Helix | 22 – 37 | 16 | ||||||||||||||||||||||||||||||||||||
| Helix | 44 – 54 | 11 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 55 – 57 | 3 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 68 – 71 | 4 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 76 – 78 | 3 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 88 – 91 | 4 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 96 – 98 | 3 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 109 – 114 | 6 | ||||||||||||||||||||||||||||||||||||
| Turn | 119 – 121 | 3 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 127 – 129 | 3 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 132 – 134 | 3 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 172 – 175 | 4 | ||||||||||||||||||||||||||||||||||||
| Turn | 176 – 179 | 4 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 180 – 184 | 5 | ||||||||||||||||||||||||||||||||||||
| Helix | 187 – 189 | 3 | ||||||||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The amino acid sequence of thiogalactoside transacetylase of Escherichia coli." Fowler A.V., Hediger M.A., Musso R.E., Zabin I. Biochimie 67:101-108(1985) [PubMed: 3922433] [Abstract] Cited for: PROTEIN SEQUENCE. |
| [2] | "DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region." Hediger M.A., Johnson D.F., Nierlich D.P., Zabin I. Proc. Natl. Acad. Sci. U.S.A. 82:6414-6418(1985) [PubMed: 3901000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [5] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [6] | "Sequence of the lactose permease gene." Buechel D.E., Gronenborn B., Mueller-Hill B. Nature 283:541-545(1980) [PubMed: 6444453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26. |
| [7] | "Structure of the lac operon galactoside acetyltransferase." Wang X.G., Olsen L.R., Roderick S.L. Structure 10:581-588(2002) [PubMed: 11937062] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS). |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| J01636 Genomic DNA. Translation: AAA24055.1. X51872 Genomic DNA. Translation: CAA36162.1. U73857 Genomic DNA. Translation: AAB18066.1. U00096 Genomic DNA. Translation: AAC73445.1. AP009048 Genomic DNA. Translation: BAE76124.1. V00295 Genomic DNA. Translation: CAA23572.1. | |||||||||||||||||||||||||||||||
| PIR | XXECTG. A94061. | ||||||||||||||||||||||||||||||
| RefSeq | AP_000994.1. NP_414876.1. | ||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||
| DIP | DIP:10078N. | ||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||
| GeneID | 945674. | ||||||||||||||||||||||||||||||
| GenomeReviews | Gene locus JW0333 in contig AP009048_GR. Gene locus b0342 in contig U00096_GR. | ||||||||||||||||||||||||||||||
| KEGG | ecj:JW0333. eco:b0342. | ||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||
| EchoBASE | EB0519. | ||||||||||||||||||||||||||||||
| EcoGene | EG10524. lacA. | ||||||||||||||||||||||||||||||
| CMR | Search... | ||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||
| HOGENOM | P07464. | ||||||||||||||||||||||||||||||
| OMA | P07464. ADVHIEA. | ||||||||||||||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||||||||||||||
| BioCyc | EcoCyc:GALACTOACETYLTRAN-MON. MetaCyc:GALACTOACETYLTRAN-MON. | ||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||
| InterPro | IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. [Graphical view] | ||||||||||||||||||||||||||||||
| Pfam | PF00132. Hexapep. 3 hits. [Graphical view] | ||||||||||||||||||||||||||||||
| PROSITE | PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||
Entry information
| Entry name | THGA_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P07464 Secondary accession number(s): P77862, Q2MC82 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


