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P07456 (IGF2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin-like growth factor II

Short name=IGF-II
Alternative name(s):
Erythrotropin

Cleaved into the following 2 chains:

  1. Insulin-like growth factor II
  2. Preptin
Gene names
Name:IGF2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length179 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.

Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3 By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the insulin family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
Osteogenesis
   Cellular componentSecreted
   DomainSignal
   Molecular functionGrowth factor
Hormone
Mitogen
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processexocrine pancreas development

Inferred from electronic annotation. Source: Ensembl

glucose metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

insulin receptor signaling pathway via phosphatidylinositol 3-kinase

Inferred from electronic annotation. Source: Ensembl

organ morphogenesis

Inferred from electronic annotation. Source: Ensembl

osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of MAPK cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of activated T cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell division

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of glycogen (starch) synthase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of glycogen biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of insulin receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of mitosis

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein kinase B signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

striated muscle cell differentiation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionprotein serine/threonine kinase activator activity

Inferred from electronic annotation. Source: Ensembl

receptor activator activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.3 Ref.5
Chain25 – 9167Insulin-like growth factor II
PRO_0000015711
Propeptide92 – 17988E peptide
PRO_0000015712
Peptide93 – 12634Preptin
PRO_0000370373

Regions

Region25 – 5228B
Region53 – 6412C
Region65 – 8521A
Region86 – 916D

Amino acid modifications

Disulfide bond33 ↔ 71 By similarity
Disulfide bond45 ↔ 84 By similarity
Disulfide bond70 ↔ 75 By similarity

Experimental info

Sequence conflict46 – 472GD → DG AA sequence Ref.5
Sequence conflict581R → L in ABD34310. Ref.1
Sequence conflict591I → S AA sequence Ref.3
Sequence conflict1681T → I in CAA37620. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P07456 [UniParc].

Last modified May 30, 2006. Version 4.
Checksum: DEA16ED2D792EA53

FASTA17919,682
        10         20         30         40         50         60 
MGITAGKSVL VLLAFLAFAS CCYAAYRPSE TLCGGELVDT LQFVCGDRGF YFSRPSSRIN 

        70         80         90        100        110        120 
RRSRGIVEEC CFRSCDLALL ETYCATPAKS ERDVSASTTV LPDDVTAYPV GKFFQYDIWK 

       130        140        150        160        170 
QSTQRLRRGL PAFLRARRGR TLAKELEALR EAKSHRPLIA LPTQDPATHG GASSKASSD 

« Hide

References

« Hide 'large scale' references
[1]"Promoter-specific expression of the imprinted IGF2 gene in cattle (Bos taurus)."
Curchoe C., Zhang S., Bin Y., Zhang X., Yang L., Feng D., O'Neill M., Tian X.C.
Biol. Reprod. 73:1275-1281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Ascending colon.
[3]"Insulin-like growth factors I and II in fetal and adult bovine serum. Purification, primary structures, and immunological cross-reactivities."
Honegger A., Humbel R.E.
J. Biol. Chem. 261:569-575(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-91.
[4]"Insulin-like growth factors 1 and 2 in bovine colostrum. Sequences and biological activities compared with those of a potent truncated form."
Francis G.L., Upton F.M., Ballard F.J., McNeil K.A., Wallace J.C.
Biochem. J. 251:95-103(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[5]"A heparin-binding erythroid cell stimulating factor from fetal bovine serum has the N-terminal sequence of insulin-like growth factor II."
Li Q., Blacher R., Esch F., Congote L.F.
Biochem. Biophys. Res. Commun. 166:557-561(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-55.
[6]"The nucleotide and deduced amino acid sequences of insulin-like growth factor II cDNAs from adult bovine and fetal sheep liver."
Brown W.M., Dziegielewska K.M., Foreman R.C., Saunders N.R.
Nucleic Acids Res. 18:4614-4614(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-179.
Tissue: Liver.
[7]"Nucleotide sequence of the central coding region of bovine erythrotropin/insulin-like growth factor II cDNA from fetal intestine and northern analysis of the major IGF II transcripts at the time of hepatic erythropoiesis."
Congote L.F., Mazza L., Palfree R.G.E.
Comp. Biochem. Physiol. 103B:127-131(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-86.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY957981 mRNA. Translation: ABD34310.1.
BC116039 mRNA. Translation: AAI16040.1.
X53553 mRNA. Translation: CAA37620.1.
X53867 mRNA. Translation: CAA37861.1.
PIRIGBO2. S10983.
RefSeqNP_776512.2. NM_174087.3.
XP_005227330.1. XM_005227273.1.
XP_005227331.1. XM_005227274.1.
XP_005227332.1. XM_005227275.1.
XP_005227333.1. XM_005227276.1.
XP_005227334.1. XM_005227277.1.
XP_005227335.1. XM_005227278.1.
UniGeneBt.106739.

3D structure databases

ProteinModelPortalP07456.
SMRP07456. Positions 25-91.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP07456.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000017372; ENSBTAP00000017372; ENSBTAG00000013066.
ENSBTAT00000044139; ENSBTAP00000041652; ENSBTAG00000013066.
GeneID281240.
KEGGbta:281240.

Organism-specific databases

CTD3481.

Phylogenomic databases

eggNOGNOG44017.
GeneTreeENSGT00530000063856.
HOGENOMHOG000233362.
HOVERGENHBG006137.
InParanoidP07456.
KOK13769.
OMARYRWQAE.
OrthoDBEOG7TF7CG.
TreeFamTF332820.

Family and domain databases

Gene3D1.10.100.10. 1 hit.
InterProIPR022334. IGF2.
IPR013576. IGF2_C.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamPF08365. IGF2_C. 1 hit.
PF00049. Insulin. 2 hits.
[Graphical view]
PRINTSPR02002. INSLNLIKEGF.
PR02006. INSLNLIKEGF2.
PR00276. INSULINFAMLY.
ProDomPD005188. IGF2_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMSSF56994. SSF56994. 1 hit.
PROSITEPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805285.

Entry information

Entry nameIGF2_BOVIN
AccessionPrimary (citable) accession number: P07456
Secondary accession number(s): A0A5F2, Q2HJN0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: May 30, 2006
Last modified: May 14, 2014
This is version 114 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families