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Protein

Insulin-like growth factor II

Gene

IGF2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3 (By similarity).By similarity

GO - Molecular functioni

  1. protein serine/threonine kinase activator activity Source: Ensembl
  2. receptor activator activity Source: Ensembl

GO - Biological processi

  1. exocrine pancreas development Source: Ensembl
  2. glucose metabolic process Source: UniProtKB-KW
  3. insulin receptor signaling pathway via phosphatidylinositol 3-kinase Source: Ensembl
  4. organ morphogenesis Source: Ensembl
  5. osteoblast differentiation Source: Ensembl
  6. positive regulation of activated T cell proliferation Source: Ensembl
  7. positive regulation of glycogen (starch) synthase activity Source: Ensembl
  8. positive regulation of glycogen biosynthetic process Source: Ensembl
  9. positive regulation of insulin receptor signaling pathway Source: Ensembl
  10. positive regulation of MAPK cascade Source: Ensembl
  11. positive regulation of mitosis Source: Ensembl
  12. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  13. positive regulation of protein kinase B signaling Source: Ensembl
  14. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  15. striated muscle cell differentiation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Hormone, Mitogen

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism, Osteogenesis

Enzyme and pathway databases

ReactomeiREACT_201972. SHC-related events triggered by IGF1R.
REACT_205176. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
REACT_206584. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
REACT_225366. IRS-related events triggered by IGF1R.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor II
Short name:
IGF-II
Alternative name(s):
Erythrotropin
Cleaved into the following 2 chains:
Gene namesi
Name:IGF2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 29

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: Ensembl
  2. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24242 PublicationsAdd
BLAST
Chaini25 – 9167Insulin-like growth factor IIPRO_0000015711Add
BLAST
Propeptidei92 – 17988E peptidePRO_0000015712Add
BLAST
Peptidei93 – 12634PreptinPRO_0000370373Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 71By similarity
Disulfide bondi45 ↔ 84By similarity
Disulfide bondi70 ↔ 75By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PRIDEiP07456.

Expressioni

Gene expression databases

ExpressionAtlasiP07456. baseline and differential.

Structurei

3D structure databases

ProteinModelPortaliP07456.
SMRiP07456. Positions 25-91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 5228BAdd
BLAST
Regioni53 – 6412CAdd
BLAST
Regioni65 – 8521AAdd
BLAST
Regioni86 – 916D

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG44017.
GeneTreeiENSGT00530000063856.
HOGENOMiHOG000233362.
HOVERGENiHBG006137.
InParanoidiP07456.
KOiK13769.
OMAiFFQYDTW.
OrthoDBiEOG7TF7CG.
TreeFamiTF332820.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR022334. IGF2.
IPR013576. IGF2_C.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF08365. IGF2_C. 1 hit.
PF00049. Insulin. 2 hits.
[Graphical view]
PRINTSiPR02002. INSLNLIKEGF.
PR02006. INSLNLIKEGF2.
PR00276. INSULINFAMLY.
ProDomiPD005188. IGF2_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07456-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGITAGKSVL VLLAFLAFAS CCYAAYRPSE TLCGGELVDT LQFVCGDRGF
60 70 80 90 100
YFSRPSSRIN RRSRGIVEEC CFRSCDLALL ETYCATPAKS ERDVSASTTV
110 120 130 140 150
LPDDVTAYPV GKFFQYDIWK QSTQRLRRGL PAFLRARRGR TLAKELEALR
160 170
EAKSHRPLIA LPTQDPATHG GASSKASSD
Length:179
Mass (Da):19,682
Last modified:May 30, 2006 - v4
Checksum:iDEA16ED2D792EA53
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 472GD → DG AA sequence (PubMed:2302223)Curated
Sequence conflicti58 – 581R → L in ABD34310. (PubMed:16120826)Curated
Sequence conflicti59 – 591I → S AA sequence (PubMed:3941093)Curated
Sequence conflicti168 – 1681T → I in CAA37620. (PubMed:2388846)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY957981 mRNA. Translation: ABD34310.1.
BC116039 mRNA. Translation: AAI16040.1.
X53553 mRNA. Translation: CAA37620.1.
X53867 mRNA. Translation: CAA37861.1.
PIRiS10983. IGBO2.
RefSeqiNP_776512.2. NM_174087.3.
XP_005227330.1. XM_005227273.1.
XP_005227331.1. XM_005227274.1.
XP_005227332.1. XM_005227275.1.
XP_005227333.1. XM_005227276.1.
XP_005227334.1. XM_005227277.1.
XP_005227335.1. XM_005227278.1.
UniGeneiBt.106739.

Genome annotation databases

EnsembliENSBTAT00000017372; ENSBTAP00000017372; ENSBTAG00000013066.
ENSBTAT00000044139; ENSBTAP00000041652; ENSBTAG00000013066.
GeneIDi281240.
KEGGibta:281240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY957981 mRNA. Translation: ABD34310.1.
BC116039 mRNA. Translation: AAI16040.1.
X53553 mRNA. Translation: CAA37620.1.
X53867 mRNA. Translation: CAA37861.1.
PIRiS10983. IGBO2.
RefSeqiNP_776512.2. NM_174087.3.
XP_005227330.1. XM_005227273.1.
XP_005227331.1. XM_005227274.1.
XP_005227332.1. XM_005227275.1.
XP_005227333.1. XM_005227276.1.
XP_005227334.1. XM_005227277.1.
XP_005227335.1. XM_005227278.1.
UniGeneiBt.106739.

3D structure databases

ProteinModelPortaliP07456.
SMRiP07456. Positions 25-91.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP07456.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000017372; ENSBTAP00000017372; ENSBTAG00000013066.
ENSBTAT00000044139; ENSBTAP00000041652; ENSBTAG00000013066.
GeneIDi281240.
KEGGibta:281240.

Organism-specific databases

CTDi3481.

Phylogenomic databases

eggNOGiNOG44017.
GeneTreeiENSGT00530000063856.
HOGENOMiHOG000233362.
HOVERGENiHBG006137.
InParanoidiP07456.
KOiK13769.
OMAiFFQYDTW.
OrthoDBiEOG7TF7CG.
TreeFamiTF332820.

Enzyme and pathway databases

ReactomeiREACT_201972. SHC-related events triggered by IGF1R.
REACT_205176. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
REACT_206584. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
REACT_225366. IRS-related events triggered by IGF1R.

Miscellaneous databases

NextBioi20805285.

Gene expression databases

ExpressionAtlasiP07456. baseline and differential.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR022334. IGF2.
IPR013576. IGF2_C.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF08365. IGF2_C. 1 hit.
PF00049. Insulin. 2 hits.
[Graphical view]
PRINTSiPR02002. INSLNLIKEGF.
PR02006. INSLNLIKEGF2.
PR00276. INSULINFAMLY.
ProDomiPD005188. IGF2_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Promoter-specific expression of the imprinted IGF2 gene in cattle (Bos taurus)."
    Curchoe C., Zhang S., Bin Y., Zhang X., Yang L., Feng D., O'Neill M., Tian X.C.
    Biol. Reprod. 73:1275-1281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.
  3. "Insulin-like growth factors I and II in fetal and adult bovine serum. Purification, primary structures, and immunological cross-reactivities."
    Honegger A., Humbel R.E.
    J. Biol. Chem. 261:569-575(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-91.
  4. "Insulin-like growth factors 1 and 2 in bovine colostrum. Sequences and biological activities compared with those of a potent truncated form."
    Francis G.L., Upton F.M., Ballard F.J., McNeil K.A., Wallace J.C.
    Biochem. J. 251:95-103(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  5. "A heparin-binding erythroid cell stimulating factor from fetal bovine serum has the N-terminal sequence of insulin-like growth factor II."
    Li Q., Blacher R., Esch F., Congote L.F.
    Biochem. Biophys. Res. Commun. 166:557-561(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-55.
  6. "The nucleotide and deduced amino acid sequences of insulin-like growth factor II cDNAs from adult bovine and fetal sheep liver."
    Brown W.M., Dziegielewska K.M., Foreman R.C., Saunders N.R.
    Nucleic Acids Res. 18:4614-4614(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-179.
    Tissue: Liver.
  7. "Nucleotide sequence of the central coding region of bovine erythrotropin/insulin-like growth factor II cDNA from fetal intestine and northern analysis of the major IGF II transcripts at the time of hepatic erythropoiesis."
    Congote L.F., Mazza L., Palfree R.G.E.
    Comp. Biochem. Physiol. 103B:127-131(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-86.

Entry informationi

Entry nameiIGF2_BOVIN
AccessioniPrimary (citable) accession number: P07456
Secondary accession number(s): A0A5F2, Q2HJN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: May 30, 2006
Last modified: January 7, 2015
This is version 118 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.